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Transcript of LINKAGE AND GENETIC MAPPING IN EUKARYOTES. In eukaryotic species, each linear chromosome contains a...
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LINKAGE AND GENETIC MAPPING IN EUKARYOTES
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In eukaryotic species, each linear chromosome contains a long piece of DNA A typical chromosome contains many hundred
or even a few thousand different genes The term linkage has two related
meanings 1. Two or more genes can be located on the
same chromosome 2. Genes that are close together tend to be
transmitted as a unit
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LINKAGE AND CROSSING OVER
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Chromosomes are called linkage groups They contain a group of genes that are linked together
The number of linkage groups is the number of types of chromosomes of the species For example, in humans
22 autosomal linkage groups An X chromosome linkage group A Y chromosome linkage group
Genes that are far apart on the same chromosome may independently assort from each other This is due to crossing-over
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Crossing Over May Produce Recombinant Phenotypes
In diploid eukaryotic species, linkage can be altered during meiosis as a result of crossing over
Crossing over Occurs during prophase I of meiosis at the
bivalent stage Non-sister chromatids of homologous
chromosomes exchange DNA segments
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Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Diploid cell afterchromosome replication
Meiosis
Possible haploid cells(a) Without crossing over, linked alleles segregate together.
ba
ba
ba
BA
ba
BA
Diploid cell afterchromosome replication
Meiosis
Possible haploid cells(b) Crossing over can reassort linked alleles.
bA
BA
ba
Ba
BA
BA
BA
ba
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5-7Figure 5.1
These haploid cells contain a combination of alleles NOT
found in the original chromosomes
These are termed parental or non-recombinant cells
This new combination of alleles is a result of
genetic recombination
These are termed nonparental or recombinant
cells
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Bateson and Punnett Discovered Two Traits That Did Not Assort Independently
In 1905, William Bateson and Reginald Punnett conducted a cross in sweet pea involving two different traits Flower color and pollen shape
This is a dihybrid cross that is expected to yield a 9:3:3:1 phenotypic ratio in the F2 generation However, Bateson and Punnett obtained surprising
results
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Figure 5.2
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A much greater proportion of the two types found in the parental generation
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Morgan Provided Evidence for the Linkage of Several X-linked Genes
The first direct evidence of linkage came from studies of Thomas Hunt Morgan
Morgan investigated several traits that followed an X-linked pattern of inheritance
Figure 5.3 illustrates an experiment involving three traits Body color Eye color Wing length
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yy ww mm
y+y w+w m+m
F1 generation
x
y w m Y
x
y+ w+ m+ Y
F1 generation contains wild-typefemales and yellow-bodied,white-eyed, miniature-wingedmales.
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Morgan observed a much higher proportion of the combinations of traits found in the parental generation
P Males
P Females
Morgan’s explanation: All three genes are located on the X chromosome Therefore, they tend to be transmitted together as a unit
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1. Why did the F2 generation have a significant number of nonparental combinations?
2. Why was there a quantitative difference between the various nonparental combinations?
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Morgan Provided Evidence for the Linkage of Several X-linked Genes
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Gray body, red eyes 1,159
Yellow body, white eyes 1,017
Gray body, white eyes 17
Yellow body, red eyes 12
Total 2,205
Let’s reorganize Morgan’s data by considering the pairs of genes separately
Red eyes, normal wings 770
White eyes, miniature wings 716
Red eyes, miniature wings 401
White eyes, normal wings 318
Total 2,205
It was fairly common to get this nonparental combination
But this nonparental combination was rare
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Morgan made three important hypotheses to explain his results 1. The genes for body color, eye color and
wing length are all located on the X-chromosome
They tend to be inherited together 2. Due to crossing over, the homologous X
chromosomes (in the female) can exchange pieces of chromosomes
This created new combination of alleles 3. The likelihood of crossing over depends on
the distance between the two genes Crossing over is more likely to occur between two
genes that are far apart from each other5-17
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Figure 5-5 Copyright © 2006 Pearson Prentice Hall, Inc.
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Figure 5.4
These parental phenotypes are the most common offspring
because the genes are far apart
These recombinant offspring are not uncommon
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Figure 5.4
because the genes are very close together
These recombinant offspring are fairly uncommon
These recombinant offspring are very unlikely1 out of 2,205
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This method is frequently used to determine if the outcome of a dihybrid cross is consistent with linkage or independent assortment
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Chi Square Analysis
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Parentalchromosomes
Nonparentalchromosomes
C Wx
c wx
c Wx
C wx
Crossing over
(b) Crossing over between normal and abnormal chromosome 9
Normalchromosome 9
Abnormalchromosome 9
Knob
(a) Normal and abnormal chromosome 9
Interchangedpiece fromchromosome 8
Creighton and McClintock ExperimentThey demonstrated physical evidence of cross-overs.
C = Coloredc = colorlessWx = Starchy endospermwx = waxy endosperm
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5-30Figure 5.6
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Interpreting the Data
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Parent A Parent B
C wx (nonrecombinant)
c Wx (nonrecombinant)
C Wx (recombinant)
c wx (recombinant)
c Wx
c wx
By combining these gametes into a Punnett square, the following types of offspring can be produced
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The colored, waxy phenotype (Cc wxwx) can occur only if Recombination did not occur in parent A
AND Parent A passed the knobbed, translocated
chromosome to its offspring This was the case, as shown in the data table
below
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The colorless, waxy phenotype (cc wxwx) can occur only if Recombination did occur in parent A
AND Parent A passed a chromosome 9 that had a
translocation but was knobless This was the case, as shown in the data table
below
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The Data
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These observations were consistent with the idea that a cross over occurred between the C and wx genes
As stated by Creighton and McClintock: “Pairing chromosomes, heteromorphic in two
regions, have been shown to exchange parts at the same time they exchange genes assigned to these regions.”
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Genetic mapping is also known as gene mapping or chromosome mapping
Its purpose is to determine the linear order of linked genes along the same chromosome
Figure 5.8 illustrates a simplified genetic linkage map of Drosophila melanogaster
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GENETIC MAPPING IN PLANTS AND ANIMALS
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Figure 5.8
Each gene has its own unique locus at a particular site
within a chromosome
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Physical Maps
Use nucleotide sequences to map genes
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Experimentally, the percentage of recombinant offspring is correlated with the distance between the two genes If the genes are far apart many recombinant offspring If the genes are close very few recombinant offspring
Map distance =
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Number of recombinant offspring
Total number of offspringX 100
The units of distance are called map units (mu) They are also referred to as centiMorgans (cM)
One map unit is equivalent to 1% recombination frequency
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5-47Figure 5.9
Chromosomes are the product of a crossover during
meiosis in the heterozygous parent
Recombinant offspring are fewer
in number than nonrecombinant
offspring
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The data at the bottom of Figure 5.9 can be used to estimate the distance between the two genes
Map distance =
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Number of recombinant offspring
Total number of offspringX 100
76 + 75
542 + 537 + 76 + 75X 100=
= 12.3 map units
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The first genetic map was constructed in 1911 by Alfred Sturtevant He was an undergraduate who spent time in the
laboratory of Thomas Hunt Morgan Sturtevant wrote:
“In conversation with Morgan … I suddenly realized that the variations in the length of linkage, already attributed by Morgan to differences in the spatial orientation of the genes, offered the possibility of determining sequences [of different genes] in the linear dimension of the chromosome. I went home and spent most of the night (to the neglect of my undergraduate homework) in producing the first chromosome map, which included the sex-linked genes, y, w, v, m, and r, in the order and approximately the relative spacing that they still appear on the standard maps.”
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Alfred Sturtevant’s Experiment
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5-52Figure 5.10
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The Data
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Alleles Concerned
Number Recombinant/ Total Number
Percent Recombinant Offspring
y and w/w-e 214/21,736 1.0
y and v 1,464/4,551 32.2
y and r 115/324 35.5
y and m 260/693 37.5
w/w-e and v 471/1,584 29.7
w/w-e and r 2,062/6,116 33.7
w/w-e and m 406/898 45.2
v and r 17/573 3.0
v and m 109/405 26.9
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Interpreting the Data
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In some dihybrid crosses, the percentage of nonparental (recombinant) offspring was rather low For example, there’s only 1% recombinant offspring in
the crosses involving the y and w or w-e alleles This suggests that these two genes are very close
together Other dihybrid crosses showed a higher
percentage of nonparental offspring For example, crosses between the v and m alleles
produced 26.9% recombinant offspring This suggests that these two genes are farther apart
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Sturtevant assumed that the map distances would be more accurate among genes that are closely linked. Therefore, his map is based on the following distances
y – w (1.0), w – v (29.7), v – r (3.0) and v – m (26.9) Sturtevant also considered map distances amongst
gene pairs to deduce the order of genes Percentage of crossovers between w and r was 33.7 Percentage of crossovers between w and v was 29.7 Percentage of crossovers between v and r was 3.0 Therefore, the gene order is w – v – r
Where v is closer to r than it is to w
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Sturtevant collectively considered all these data and proposed the following genetic map
Sturtevant began at the y gene and mapped the genes from left to right
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A close look at Sturtevant’s data reveals two points that do not agree very well with his genetic map The y and m dihybrid cross yielded 37.5% recombinants
But the map distance is 57.6 The w and m dihybrid cross yielded 45.2% recombinants
But the map distance is 56.6
So what’s up? As the percentage of recombinant offspring
approaches a value of 50 % This value becomes a progressively more
inaccurate measure of map distance Refer to Figure 5.11
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When the distance between two genes is large The likelihood of multiple crossovers increases This causes the observed number of recombinant offspring
to underestimate the distance between the two genes
Figure 5.11
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Figure 5-12a Copyright © 2006 Pearson Prentice Hall, Inc.
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Trihybrid Crosses
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Data from trihybrid crosses can also yield information about map distance and gene order
The following experiment outlines a common strategy for using trihybrid crosses to map genes In this example, we will consider fruit flies that differ in
body color, eye color and wing shape
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Trihybrid Crosses
b = black body color b+ = gray body color
pr = purple eye color pr+ = red eye color
vg = vestigial wings vg+ = normal wings
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Step 1: Cross two true-breeding strains that differ with regard to three alleles.
Female is mutant for all three traits
Male is homozygous wildtype for all three
traits
The goal in this step is to obtain aF1 individuals that are heterozygous for all three genes
Order of genes not important here.
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Step 2: Perform a testcross by mating F1 female heterozygotes to male flies that are homozygous recessive for all three alleles
During gametogenesis in the heterozygous female F1 flies, crossovers may produce new combinations of the 3 alleles
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Step 3: Collect data for the F2 generation
Phenotype Number of Observed Offspring
Gray body, red eyes, normal wings
+ + +
411 parental
Gray body, red eyes, vestigial wings
+ + vg
61 pr/vg
Gray body, purple eyes, normal wings
+ pr +
2 b/pr and pr/vg
Gray body, purple eyes, vestigial wings
+ pr vg
30 b/pr
Black body, red eyes, normal wings
b + +
28 b/pr
Black body, red eyes, vestigial wings
B + vg 1 b/pr and pr/vg
Black body, purple eyes, normal wings
B pr +
60 pr/vg
Black body, purple eyes, vestigial wings
B pr vg
412 parental
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The three genes exist as two alleles each Therefore, there are 23 = 8 possible combinations
of F2 offspring If the genes assorted independently, all eight
combinations would occur in equal proportions It is obvious that they are far from equal
In the offspring of crosses involving linked genes, Parental phenotypes occur most frequently Double crossover phenotypes occur least frequently Single crossover phenotypes occur with
“intermediate” frequency
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The combination of traits in the double crossover tells us which gene is in the middle A double crossover separates the gene in the middle from
the other two genes at either end
In the double crossover categories, the recessive purple eye color is separated from the other two recessive alleles Thus, the gene for eye color lies between the genes for
body color and wing shape
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Which are the double cross-overs?
The ones with the least amount.
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Step 4: Calculate the map distance between pairs of genesNumber of recombs between pr and vg: 61 + 60+ 2 + 1 = 124Number of recombs between b and pr: 30 + 28 + 2 + 1 = 61Number of recombs between b and vg, all but double cross-overs: 61 + 60 + 30 + 28 = 178
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Map Distance
pr/vg = 124/1005 x 100 = 12.3 b/pr = 61/1005 x 100 = 6 b and vg = 179/1005 x 100 = 17.8
_____6____________12.3____________ b pr vg
The distance between b and vg was found to be 17.8. The actual distance is 18.3 mu.
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Interference The slightly smaller lower value was a
small underestimate because we did not consider the double crossovers in the calculation between b and vg.
The lower than expected value is due to a common genetic phenomenon, termed positive interference. The first crossover decreases the probability that a second crossover will occur nearby.
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Much of our earliest understanding of genetic recombination came from the genetic analyses of fungi
Fungi may be unicellular or multicellular organisms They are typically haploid (1n) They reproduce asexually and, in many cases,
sexually
The sac fungi (ascomycetes) have been particularly useful to geneticists because of their unique style of sexual reproduction
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GENETIC MAPPING IN HAPLOID EUKARYOTES
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Meiosis produces four haploid cells,
termed spores
Figure 5.12
These are enclosed in a sac termed an ascus
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The cells of a tetrad or octad are contained within a sac
In other words, the products of a single meiotic division are contained within one sac
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The arrangement of spores within an ascus varies from species to species Unordered tetrads or octads
Ascus provides enough space for the spores to randomly mix together
Ordered tetrads or octads Ascus is very tight, thereby preventing spores from
randomly moving around
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Types of Tetrads or Octads
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5-82Figure 5.13
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YeastUnicellular alga
Mold
Ascus provides space for spores to
randomly mix together
Tight ascus prevents mixing
of spores
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Ordered tetrads or octads have the following key feature The position and order of spores within the ascus is
determined by the divisions of meiosis and mitosis In crosses of tan and black Neurospora cultures, the
spores appear tan or black in a certain order. All black spores or all tan spores indicate no
hybridization.
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Ordered Tetrad Analysis
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5-84Figure 5.13
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Pairs of daughter cells are located
next to each other
All eight cells are arranged in a linear, ordered fashion
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020
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Non-crossovers
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Cross-overs
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Non cross-overs
Cross-overs
Non cross-overs
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To calculate this distance, the experimenter must count the number of cross-over asci, as well as the total number of asci In cross-over asci, only half of the spores are actually the
product of a crossover Therefore
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(1/2) (Number of SDS asci)
Total number of asciX 100Map distance =
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Genetics of Corn
http://media.hhmi.org/biointeractive/films/PoppedSecret.html