Lienhard Pagel 4.6.2007 Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of...
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Transcript of Lienhard Pagel 4.6.2007 Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of...
Lienhard Pagel4.6.2007
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Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of computer since and electronicsInstitute of Electronic Appliances and Circuits, University of Rostock, Rostock, Germany
Lienhard Pagel4.6.2007
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Micro fluidics in PCB technology
Electronic area IC's, resistor, capacitor, plug and socket connection, push-button, display, ...
Fluidic area:
pumps, valves, channels, filter, ... cavities, optical sensors, conductivity measurement, …
Electronic area
Fluidic area
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University of RostockPCB-Fluidics
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Fluidic PCB-Technology
normal PCB
production
drilling milling
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Fluidic PCB-Technology
normal PCB
production
etching
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Fluidic PCB-Technology
second PCB stacked
copper lines build channels
gluing
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Fluidic PCB-Technology
tube connection
channel view
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Fluidic PCB-Technology
cross section of channel
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Cross section of channel wall
epoxy
copper (2)
copper (1)channel
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9µC controlled programmable micro pump
Electrical connector- Power 4 –7 V DC- I2C - Bus - programming
500µl/min Flow 150 mBar max Backpressure
over USB-I2C IF fully programmable
Lienhard Pagel4.6.2007
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Flow-Injection-Analysis
quantitative measurement of components automated procedure short response times / high sample
throughput usage in quality control / automated
assays developed by J. Růžička and E.H. Hansen
in the year 1975
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Flow-Injection-Analysis
Sample
Reaction coil
Injection valve Detector
Reagent
Pump
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Reaction
Detection of Fe3+
Thiocyanate as reagent forms red colored Iron-Thiocyanate detection by absorption at 480 nm
SCNFe 33
3)(SCNFe
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Why a FIA?
All basic fluidic handling necessary- transportation of fluids- dosing of fluids- mixing- detecting- adoption to detection needed- electronics needed
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FIA in PCB-Technologie Principle
reagentpump
sample pump
reaction coil
injection detector
• 2 separate controllable pumps • no injection valve• optical detection
photometrical 480 nm
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MIXINGMIXINGFlow pattern in 180° curves
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Simulation results of the used reaction channel Simulation results of the used reaction channel
Parameters: 2 pumps with constant flow of 0.2ml/min
Fluidsimulation:FLOWORKS789388 cells
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… and the reality
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Simulation results of the used reaction channel Simulation results of the used reaction channel
parameters:thermopneumatically actuated pumps
with pulsing flow of 0.2ml/min,
789388 cells
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Simulation results of the reaction channelSimulation results of the reaction channel
vector plot of the fluid velocity at the cross section of the channel after the second curve
parameters: thermopneumatically actuated pumps with pulsing flow of 0.2ml/min,
789388 cells
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OPTICAL SENSOROPTICAL SENSOR in FIA
Pho to d io d e
O p tic a l p a th
1m m
Sim ula tio n Flo w 5 m l/m in!
LED
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Optical Cell with air bubble (vector of the force)Optical Cell with air bubble (vector of the force)
Sim ula tio n Flo w 5 m l/m in!
F
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Realization
5 cm
5 cm
• stack of four PCBs • one movable layer of polyimid• electronics on the back of the fourth PCB
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FIA Realization
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FIA in PCB with integrated electronics
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Signals in the photo detector in dependence on the concentration of Fe(III)ions
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PCB FIA facts
Detection Fe3+ Ions0,16 mMol – 10 mMolreproducibility peak height 5%, peak area 10%
Monolithic system consisting fluidics and electronics in one stack of PCBs.
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Thank you for your attention!Thank you for your attention!