LI-6800 Bryophyte Chamber Installation Guide
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Installing the Bryophyte Chamber The Bryophyte Chamber (6800-24) is used to measure CO 2 and H 2 O gas exchange from mosses, hornworts, liverworts, and lichens. The chamber holds sample material on wire mesh or in a shallow well during the measurement. It fea- tures a clear Propafilm™ top for measurements under ambi- ent lighting conditions. It is compatible with the Large Light Source for measurements under controlled lighting with any combination of red, green, blue, and white light. This doc- ument provides installation instructions for the chamber. Description Part Number Mesh Screen (1) 197-09025 Short Risers (4) 170-04629 Short Riser Screws; M3-8 (4) 150-13642 Tall Risers (4) 170-16503 Tall Riser Screws; M3-25 (4) 150-15454 Chamber Bottom Plate (1) 9868-550 Chamber Bottom Screws; M4-12 (4) 150-14476 Flat Washers; #4 5/16 (8) 167-00155 Detach the chamber from the head Six screws will be removed to detach a chamber — two on the connector cover, two on the latch mechanism, and two that hold the chamber to the head. This requires Phillips PH1 and PH2 screwdrivers, which are in the spares kit. Suit- able screwdrivers are available from most department stores. Caution: Power off the instrument before following these procedures. 1 Using the PH1 Phillips screwdriver, remove the two cover screws and the connector cover from the bot- tom of the head. 2 Unplug the cables from the cable bulkhead (i.e., LI-190R, leaf temperature thermocouple, and light source). 3 Position the chamber latch in the parked position and remove the two latch screws with the PH2 Phillips screwdriver. 4 Using a PH2 Phillips screwdriver, loosen both of the captive chamber screws until the chamber is free of the head. 5 Separate the chamber from the head. Warning: Do not attempt to operate the mixing fan without a chamber in place. 1 Installation Guide
Transcript of LI-6800 Bryophyte Chamber Installation Guide
Installing the Bryophyte Chamber
The Bryophyte Chamber (6800-24) is used to measure CO2and H2O gas exchange from mosses, hornworts, liverworts,and lichens. The chamber holds sample material on wiremesh or in a shallow well during the measurement. It fea-tures a clear Propafilm™ top for measurements under ambi-ent lighting conditions. It is compatible with the Large LightSource for measurements under controlled lighting with anycombination of red, green, blue, and white light. This doc-ument provides installation instructions for the chamber.
Description Part Number
Mesh Screen (1) 197-09025Short Risers (4) 170-04629Short Riser Screws; M3-8 (4) 150-13642Tall Risers (4) 170-16503Tall Riser Screws; M3-25 (4) 150-15454Chamber Bottom Plate (1) 9868-550Chamber Bottom Screws; M4-12 (4) 150-14476Flat Washers; #4 5/16 (8) 167-00155
Detach the chamber from the headSix screws will be removed to detach a chamber — two onthe connector cover, two on the latch mechanism, and twothat hold the chamber to the head. This requires PhillipsPH1 and PH2 screwdrivers, which are in the spares kit. Suit-able screwdrivers are available from most department stores.
Caution: Power off the instrument before following theseprocedures.
1 Using the PH1 Phillips screwdriver, remove the twocover screws and the connector cover from the bot-tom of the head.
2 Unplug the cables from the cable bulkhead (i.e.,LI-190R, leaf temperature thermocouple, and lightsource).
3 Position the chamber latch in the parked position andremove the two latch screws with the PH2 Phillipsscrewdriver.
4 Using a PH2 Phillips screwdriver, loosen both of thecaptive chamber screws until the chamber is free ofthe head.
5 Separate the chamber from the head.
Warning: Do not attempt to operate the mixing fanwithout a chamber in place.
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Installation Guide
While the chamber is removed from the head, inspect the AirFilters and Dust Trap. Clear any dust or debris before attachinga chamber.
Installing the Bryophyte Chamber
1 Install the chamber adapter.
2 Tighten the screws snugly—until they are tight andthen just a little bit more. Reinstall the connectorcover.
3 Remove the chamber top. It lifts off of the chamber.
4 Prepare the chamber bottom for a sample.
There are three options for loading the sample: A) on thescreen with short risers, B) on the screen with tall risers,or C) in a well. The well is on one side of the chamberbottom; the screens mount to the other side. Install thechamber bottom in the orientation that you prefer.
Figure 1. Chamber bottom configurations. A shows theshort risers; B shows the tall risers; and C shows thewell.
5 Power on the LI-6800, if it is not already on, andverify that the instrument recognizes the 6800-24chamber under Start Up > Configuration > Chamber Setup.
6 Choose whether to compute Area-based fluxes or Mass-based fluxes.
7 Press the chamber top into the opening and run theleak test (Start Up > System Tests > Chamber Leak).
Loading a sampleRemove a sample from the substrate using a knife or cookiecutter. You can place a sample on the screen or in the well. Ifyou're computing fluxes on the basis of mass, you'll need toweigh each sample and make a record of the mass. For area-based fluxes, you'll need to determine the sample area. Afterloading the sample, press the chamber top onto the chamber.
Making a measurementBelow we offer some basic settings that will work for a typ-ical measurement.
1 Set the Environment controls.
Flow: Set Flow: On; Pump speed: Auto; Flow rate: 700µmol s-1; Press Valve: 0.0 kPa
H2O: Set H2O: On; RH_air: 50%
CO2: Set CO2 injector: On; Soda Lime: Scrub Auto; TapCO2_s and enter a setpoint of 400 µmol mol-1
Fan: Set Mixing fan: On; Fan Speed: 10,000 rpm
Temperatures: Check the temperatures to see theircurrent values
2 Set the Constants.
Under Constants > Gas Exchange, enter the Area or Mass.3 Configure Logging options.
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Configure the Match Options and Logging Options as desired.Check Prompt on (manual) log, which will open a promptwhen you press the Log button, where you can enter theweight or area.
4 Open a log file.
5 Tap the Log button to record a measurement.
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