Leukocytes Leukocytes, or white blood cells, are found within the bone marrow (BM),the peripheral...
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Transcript of Leukocytes Leukocytes, or white blood cells, are found within the bone marrow (BM),the peripheral...
Leukocytes
Leukocytes, or white blood cells, are found within the bone marrow (BM),the peripheral blood, and the tissues. Leukocytes are among the essential elements of the hematopoietic-lymphoreticular-immune system, which functions to protect the human body from nonself cells (infection) and altered-self cells (cancer).
Neutrophilia
Mechanisms: (1) the rate of inflow of cells from the BM (2) the proportion of neutrophils in the marginal granulocyte pool
(MGP) and the circulating granulocyte pool (CGP) of the blood (3) the rate of outflow of neutrophils from the blood.
Determinants:1) Certain host factors 2) Microorganisms factors3) Drugs
Neutropenia
Neutropenia is a reduction in the absolute neutrophil count (ANC) below ˜1.5–2 × 10*9/L for white adults and below ˜1.2–1.3 × 10*9/L for black adults.
Mechanisms: (1) the rate of inflow of cells from the BM (2) the proportion of neutrophils in the marginal
granulocyte pool (MGP) and the circulating granulocyte pool (CGP) of the blood
(3) the rate of outflow of neutrophils from the blood.
Causes of Neutropenia
Intrinsic defects1. myeloid hypoplasia or a proliferation defect Fanconi’s anemia (FA) Kostmann’s syndrome Schwachman-Diamond syndrome cyclic neutropenia
2. maturation defects myelokathexis Chédiak-Higashi syndrome.
Kostmann’s syndrome
• The peripheral smear confirmed the leukopenia and almost complete absence of neuutrophils.
• A touch preparation done on the marrow biopsy shows a marked increase in myeloid precursors without evidence of maturation
PMN
Causes of Neutropenia
Immune-mediated: RA
Hematologic: megaloblastic anemia, myelodysplasia, marrow failure, marrow replacement
Infectious: any overwhelming infection
Others: starvation, hypersplenism
Pseudoneutropenia
• Pseudoneutropenia may be caused by increased margination of neutrophils in some individuals, without a decrease in total granulocyte count.
• Small doses of endotoxin will cause a shift of neutrophils into the MGP from the CGP, giving an apparent neutropenia, before causing leukocytosis.
Functional Disorders of Neutrophils
alterations in both morphology and function: Chédiak-Higashi syndrome specific granule deficiency (SGD)
functional disorders with essentially normal morphology on Wright’s- and Giemsastained films:
Chronic granulomatous disease (CGD) Myeloperoxidase deficiency(MYD) Leukocyte adhesion deficiency (LAD)
Myeloperoxidase Deficiency(MYD)
• Myeloperoxidase (MPO) is present in neutrophils (in azurophil/primary granules) and monocytes (in lysosomes) but is not present in promonocytes and tissue macrophages.
• (H2O2 + Cl- → H2O + ClO-)
Leukocyte Adhesion Deficiency (LAD)
• LAD-I: a mutation in the ß2-subunit (of CD18)
• LAD-II: mutation affecting fucose transport, leading to the impaired expression
of selectin ligands on leukocytes (CD15a or sialyl Lewis X)
• LAD-III: defect in integrin activation (via endothelial chemokines) caused by a
mutation in leukocyte-expressed G protein–coupled receptors (GPCRs)
Specific Granule Deficiency (SGD)
• present with multiple bacterial infections, atypical bilobed nuclei within neutrophils, and lack of secondary/specific cytoplasmic granules within neutrophils on Wright’sstained peripheral blood films.
• SGD also affects eosinophils. • granule deficiencies , impaired bactericidal activity,
neutrophils are defective in chemotaxis, receptor upregulation, and disaggregation.
• A mutation in a myeloid transcription factor gene (C/EBPe) appears to play a role in this disease.