Leica DM4000 B Leica DM5000 B - …photos.labwrench.com/equipmentManuals/6463-2193.pdf · 2...
Transcript of Leica DM4000 B Leica DM5000 B - …photos.labwrench.com/equipmentManuals/6463-2193.pdf · 2...
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IntelligenceComfort
BrillianceIntegration
This is why you entered science.It's what you work for every day.
Now let the new Leica DM DigitalMicroscope help you make
that brilliant discovery!
Leica Microsystems AG – Winner of the World’s First Innovation Award:German Business Innovation Award 2002
Photo courtesy of M. Okabe, Osaka University (J)
The Exterior: New Technology in a New Design
The first thing you will notice about our new DigitalMicroscopes
is their new design: clear, attractive contours.
Looking Through the Microscope: Fascinating Insights
Once you have seen a sample through one of these new micro-
scopes, you will never want to use any other. No microscope in
this class can offer better image brilliance, field depth and con-
trast.
Just Rely On Your Intuition
Our new Leica DM DigitalMicroscope series provides the
answers to many of our customers problems. One of the most fre-
quently voiced requests was to lighten the workload. So we found
ways of doing a lot of the work for you. You can operate the micro-
scope intuitively and easily automate complex routines to suit
your specific needs.
Experts Call it Ergonomics. We Call it User-Friendly
Ergonomics is a word often used. On our new microscopes you
can actually feel it. Cooperating closely with the Fraunhofer Insti-
tute*, our designers have not only outperformed the latest tech-
nological standards but also all the ergonomic specifications.
Our Product Range Caters To All Your Needs
Whatever you need for your examinations or research, we can
supply it: the microscopes, the cameras and even the software for
analyzing and archiving your images.
New All-Embracing Software
Parallel to the new Leica DM DigitalMicroscope series we are
offering our own totally new software concept. All our hardware
and software components are now controlled from one single
interface.
*The Fraunhofer Institute IAO (Stuttgart, Germany) investigates ergonomic aspects ofvarious products. In cooperation with their industrial partners they develop industrialdesigns to suit highest ergonomic demands.
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Perfection At Work - Simply Microscopy!
Leica DM4000 B with the basic BT25+ tube in a configurationfor fluorescence and the new fully motorizedphase contrast condenser
Your Benefits
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Intelligence Automatic Diaphragm AdjustmentOur new DigitalMicroscopes automatically recognize the con-trasting technique and objective that is currently in use. There isno need to adjust any more diaphragms – either in transmitted orin incident light.
Automatic Light AdjustmentThe light intensity is automatically set to the light-gathering powerof the objectives. This means that the brightness of the specimenimage remains constant when you switch to a different objective –and there is no danger of glare. And because every task has itsown specific requirements, you can adjust the light intensity indi-vidually.
New: Transmitted Light Axis With Constant Color Intensity Control (CCIC)Too dark for viewing – too bright for your digital camera: in thepast it was necessary to use a white balance every time the lampvoltage was changed. The new transmitted light axis works witha color-neutral brightness control which automatically maintainsa constant color temperature. So you no longer need to use neu-tral density filters to compensate for changes in light intensity.
New Condensers for a New Level of AutomationOur fully automated condensers will meet the most demandingrequirements. The motorized tops, which automatically swing inand out of the light path, are applicable from 1.25x to 100x. Themotorized condenser turret accommodates up to four interfer-ence prisms and up to four phase rings as well as the brightfieldand darkfield positions. We also offer an integrated, motorizedpolarizer for fully automatic interference contrast.
Clarity Wherever You LookAll the settings of the Leica DM4000 B can be seen at a glance inthe clearly laid out display. And the Leica DM5000 B offers younever before realized control possibilities with the completelynew touch-sensitive screen. You can control all the automatedfeatures of the microscope via the touch screen – there is noneed to know anything about programming.
An impression of clarity wherever you look: Customer-specificillumination and diaphragm adjustments can be made on the frontleft side of the microscope.
The new LeicaScreen on the DM5000 B withtouch-sensitive LCD display. The clearly designed menu structureuses internationally familiar pictograms instead of language forintuitive operation.
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Comfort
Brilliance
Our DigitalMicroscopes Adapt to You in Every WayThe new adaptable tube can be perfectly matched to your bodysize and posture. You can reach the focus knobs with your handsresting on the table. The new stage allows simultaneous focusand movement control. So whatever you are examining, you arecompletely relaxed – even if you sit at the microscope for hours ata time.
New Stages and Specimen Holders For Your ConvenienceWe have designed the new stages to satisfy the most demandingapplications: scratch-resistant thanks to the ceramic coating andfeaturing telescopic stage drives with individually adjustabletorque. To eliminate the risk of injury, we decided not to implementa rack for the y movement. The rotatable stages are suitable forone to two specimens and are available in a version for left-handedoperation on request.
Variable Function KeysYou can assign any function you like to the six new functionkeys. Due to their convenient position behind the focus wheels,frequently used functions are always within easy reach. We hadyour comfort in mind.
Five New Viewing Tubes for Pin-Sharp ImagesTo match our new DigitalMicroscopes, we have devised a view-ing tube series that will meet the highest requirements. Our newdocumentation tubes (which can be motorized on request*) havethree switching positions and are fitted with either one or twocamera ports. The new adaptable tube can be optimally adjustedto your needs. And of course, you will also find an ergonomytube with documentation port in our product range.
New 1.25x Scanning ObjectiveThe new 1.25x panoramic objective is especially intended for bio-logical sciences. In combination with our new transmitted lightaxis, it gives you excellent field depth and optimum illuminationhomogeneity for low magnification imaging.
* The motorized switchable tube MBDT25+ will be available from October 2003.
Together with the new condenser, our new transmitted light axispermits Koehler illumination from 1.25x - 100x. And the new 1.25xpanorama objective offers excellent depth of field and perfectlyhomogeneous illumination.
The viewing angle and height of the new AET22 adaptable tube canbe individually adjusted to your sitting posture and body size.Plus you can vary the length of the eyepieces to suit the positionof your arms.
Your Applications – Our Solutions
Transmitted Light
BrightfieldIt has never been so easy to use a microscope: just put a speci-men on the stage and focus! The Leica DigitalMicroscope recog-nizes the objective you have chosen, automatically sets the con-denser top, accurately opens and closes the aperture and fielddiaphragms and adapts the light intensity. The automated color-neutral brightness control also filters red and orange componentsout of the light, giving you a constant impression of your speci-men.
Phase ContrastThere is no further need to search and select phase contrastrings. The DM knows the right ring for the objective you haveselected. Each objective has its own ring, saving you the nuisanceof re-centering.
Interference ContrastThe Leica DM5000 B is the first microscope to offer totally auto-mated interference contrast. All the modules are fully motorized:analyzer, polarizer, condenser prisms and objective prisms. TheLeica DM5000 B also knows the right condenser and objectiveprisms for the objective you have chosen and moves them and thepolarizer and analyzer automatically into the light path. Themicroscope stores the fine objective prism settings for eachobjective so that they can be reproduced whenever needed.
Darkfield and PolarizationOf course, all the other contrasting techniques implementedin the Leica DM4000 B and Leica DM5000 B, such as darkfieldand polarization, are automated, too. Here again, the micro-scope knows all the necessary components.
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Cross section through tilia wood. brightfield
BPAE cells with mouse anti-α-tubulin, BODIPYL®FL goat anti-mouse IgG,Texas Red® X phalloidin, DAPI. Molecular Probes.
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Fluorescence
New: FIM Technique (Fluorescence Intensity Management)This facility for reducing the fluorescence illumination in fivesteps minimizes the problem of fading samples. It also lets youstore a separate FIM step for each fluorescence filter to obtain aconstantly bright image of multiple-stained specimens.
More Light With a Simple Hand MovementIf you need more light, simply switch on the new booster lens,which immediately directs more light to your sample if required.
5- and 8-position Filter Cube ChangerThe stands of the new Leica DigitalMicroscopes are fitted with aturret for up to five fluorescence filter cubes. The DM5000 B canalso be supplied with an 8-position filter turret on request. Bothfilters take the same type of filter cube, allowing you to switchconveniently between different versions. You have the choicebetween continuous switching of all cubes or direct selection ofindividual filter cubes.
Integrated BG38 FilterManual insertion of a BG38 filter for certain fluorescence applica-tions is no longer necessary. The function of this filter has beenintegrated on the fluorescence filter cubes.
Single Keystroke Contrast Switching -Easier Than Ever BeforeThe method of changing contrasting techniques is unique.One press of the innovative function keys and the micro-scope switches between brightfield, phase contrast,polarization, darkfield or fluorescence automatically. Inthe case of the Leica DM5000 B we have even assignedall the interference contrast settings to one single key.
Cross section through the axis of a helianthusshoot, openly collateral bundles, phase contrast
Transgenic C. elegans displaying GFP expression.Courtesy of Dr. M. Morcos,Heidelberg, Germany
Leica Design by Christophe Apothéloz
Your System Solutions
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The Choice Is Yours. Now and in the Future.To go with the new DigitalMicroscopes we offer you a totally newsoftware concept which allows you to upgrade your system atany time. All future software and hardware components of Leicawill be controlled from the same interface.
Individual Microscope Configuration and ControlThe user interface is extremely easy to use. Function keys, con-trasting techniques and other microscope parameters are easilyconfigured on the computer in accordance with your preferencesor the needs of your working environment. Operator errors arepractically impossible, which is particularly important in a routineenvironment. You can also use the software to remote-controlyour microscope.
Cameras For Every RequirementThe Leica DC range of digital cameras offers the right camera forevery requirement. From general to specific, our cameras are ideal:color or gray-level pictures of fluorescence specimens or industrialmaterials. There are cameras with a live image or real time videomode. The camera chips feature up to 12 megapixel image resolu-tion. And with exposure times between a few µs and several min-utes, every customer will find the camera for his or her application.
Metaphase-spread FISH – stained chromosomesPhotos: Dr. Yumiko Suto, Tokyo UniversityCourtesy of Dr Yumiko Suto, Biological Sciences, University of Tokyo, Japan.
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Perfect Image Archiving and AnalysisWith the Leica IM1000 archiving program you can create picturegalleries, annotate images and store microscope parameters. TheLeica FW4000 fluorescence system offers a comprehensive solu-tion for recording, processing, publishing and archiving images.In cooperation with other laboratories, a user-friendly interfacehas been designed which can be precisely tailored to specificcustomer requirements. The Leica CW4000 cytogenics programoffers solutions for karyotyping, FISH (Fluorescence In-SituHybridization), MFISH (Multi-Colored FISH) and CGH (Compara-tive Genome Hybridization), etc.
Integration
0.5x
11888080Basis stand DM4000 B/DM4000 Mincl. TL axis, incl. LED display
11505080Lamp housing12 V 100 WHalogen lamp
11501233 x/y stage ERGO incl. stage bracket and condenser holder, fixed, 1 specimen
11501234 x/y stage BIO II, oil immersion, 2 specimen
11501232 x/y stage BIO I, incl. stage bracket and condenser holder, rotatable, 2 specimen
11505163Object holder ERGO,single-hand-operation,1 specimen
11505156Object holder BIO I,single-hand-operation,1 specimen
11505157Object holder BIO II,oil immersion, 2 specimen
11505141Condenser BFfor mot. condenser top
11505142Condenser PH (usable for BF, PH, DF, ICT),for mot. Condenser top, incl. 7-foldcondenser turret, mot.
11505143Condenser DIC (usable for BF, PH, DF, ICT)for mot. Condenser top, incl. 7-foldcondenser turret, mot.,incl. mot polarizer
11888081Basic stand DM5000 Bincl. TL axis, incl. Leica-Screen
11888084mech. Focus drive
11505080Lamp housing12V 100WHalogen lamp
11505141Condenser BFfor mot. condenser top
11505142Condenser PH (usable for BF, PH, DF, ICT),for mot. Condenser top, incl. 7-foldcondenser turret, mot.
11505143Condenser DIC (usable for BF, PH, DF, ICT)for mot. Condenser top, incl. 7-foldcondenser turret, mot.,incl. mot polarizer
11888082Basicstand DM4000 Mwithout TL axis, incl. LED display
11888084mech. Focus drive
11888105Coverobjective revolvingnosepiece
11888101 Cover objective revolving nosepiece, slot for sliders
11555071 Cover objective revolving nosepiece incl. DIC prism turret 4 pos., mot.
11888105Coverobjective revolvingnosepiece
11888640Stand top DM4000 Mincl. Reflected light axis,incl. 4-fold Reflector turret, mot.,inkl. Objective revolving nosepiece6-fold M32, coded
11888088Stand top DM4000 Bincl. fluorescence axis,incl. 5-fold filter turret, mot.,incl. 6 objective revolving nosepiece6-fold M25, coded
11888092Stand top DM5000 Bwithout fluorescence axis,incl. objective revolving nosepiece7-fold M25, coded
11888086Stand top DM5000 Bincl. fluorescence axis,incl. 5-fold filter turret, mot.,incl. objective revolving nosepiece7-fold M25, coded
*11888090Stand top DM5000 Bincl. fluorescence axis,incl. 8-fold filter turret, mot.,incl. objective revolving nosepiece7-fold M25, coded
11888089Stand top DM4000 Bwithout fluoreescence axis,incl. objective revolving nosepiece6-folded M25, coded
*11888642 Top cover incl. Magnification changer for industry (1x, 1.5x, 2x), coded
11888095 Top cover
*11888096 incl. Magnification changer for bio/med (1x, 1.25x, 1.6x), coded
11505148AET22advanced ergo tubeincl. tilting and extendableeyepieces
11505149EDT22 F 50/50Ergo documentaion tubeincl. tilting eyepieces 11505147
BT25 + Basic tube
11505146BDT25 + V 100/50/0Basic documentation tubewith variable beam splitting
*11505145MBDT25 + V 100/50/0Basic documentation tubewith variable beam splitting, mot.
Image analysis/image storage
11505161Tube topincl. 1 camera port
11505162 Tube top incl. 2 camera ports
11505154incident light slider
11888124Booster-Lensfor fluorescence axisD= 25 mm
FilterD= 25 mm
various excitation manager
11561090x/y stage IND,incl. stage bracket andcondenser holder,rotatable, 76 x 50 mm
11888098Ground plate withoutfilter magazine
11888100Ground plate incl.Filter magazinefor 2 filters (D= 32 mm)
11888098Ground plate withoutfilter magazine
11888098Ground plate withoutfilter magazine
11888100Ground plate incl.Filter magazinefor 2 filters (D= 32 mm)
11888084mech. Focus drive
11888108Supply unitCTR5000
graticules(for material numbers see modular system brochure)
Eyepiece adapter HC Photo 8x, 10x, 12,5x
Eyepieces HCPhoto8x, 10x, 12,5x
Control panel MPS30
MPS30 MPS60
Shutter unitwith sensor
Control panel MPS60
Keyboard fordataback
Focusing magnifier for MPS60
11505070focusing telescope
c-mount adapters HC, (for material numbers see mod. system brochure)
TV camera without opticsMonitor
11555045Analysor ICT/P
11561075x/y stage4" x 4"
11561010Incident light stagewith reverse stagebracket for largespecimen
11562294Tilting object guide
11563546Incident light objectguidefor large specimen
ICT objektive prisms(for material numbers see modular system brochure)
Fluorescence filter systems(for material numbers see modular system brochure)
ICT condenser prisms (K1-K15)(for material numbers see modularsystem brochure)
Compensators andIC prisms in sliders
115045000Illumination telescope
11555034Polarizer ICT/P
11555066Analysor 180°rotatable
11555005Polarizer R/P
11565001Polarizer rotatable
11555051Polarizer L/ICR
11555034Polarizer ICT/P
11505075Condenser DFbottom part
11505075Condenser DFbottom part
11505150Condenser top 0.90 S1
11551004Condenser top P 1.40 Oil S1
11501037Condenser top 0.50 S15
11505152DF condenser topD= 0.80 – 0.95
11505176Set light ringsDF, PH 1/1/2/3
11561089Object holdermetall
11555069Reflector P. Smith
Micro hardness tester MHT10
Interference attachmentsMichelson/Mirau5x-50x
11505059Specimen marker
Immersion cap
11505139Reflector BF
11505140Reflector DF
11563035Hand press
115630156 metall specimen slides
Camera systemsLeica SCR camera systemLeica MPS30/60see brochure
11505061 Ergomodule
11505092 tracing device
Camera objective0,32x
Motor adapter(24 x 36) withoutoptics
Film magazine24 x 36
11505070focusing telescope
TV Systems
Tube program L
Leica DC150
Digital cameras, see brochure
Leica DC300 (F)
Leica DC350 (F)
Leica DC480 (F)
Leica DC500
ENG(
B) m
ount
HC
c-m
ount
HC
0,5-
2,4x
0,33
-1,6
x
0,35
x
0,5x
0,63
x
1x
11500277Power unitHg 50 W
11500325Power unitHg 100 W
11504063Lamp housing LH106zHg 100 W6-lens collector
11504066Lamp housing LH 106zHg 50 W4-lens collector
11504069Lamp housing LH 106zHg 100 W4-lens collector
11504053Mirror housingswitchable for 2lamp housings
11505080Lamp housing LH 107/212 V 100 W Halogen lamp
11504058Lamp housing LH 10612 V 100 W Halogen lamp0,55 m cable
11504059Lamp housing LH 10612 V 100 W Halogen lamp2 mcable
11504070Lamp housing LH 106z12 V 100 W Halogen lamp4-lens collector0,55 m cable
11504071Lamp housing LH 106z12 V 100 W Halogen lamp4-lens collector2 mcable
11504030Intermediate piecewith filter holder for2-4 filters (D= 50 mm)
*available October 2003
Objective series BF
Objective series DF
11513860Immersion oil 20 ml
11513861Immersion oil 250 ml
11513859Immersion oil,low fluorescence, 10 ml
11541043DM MFK
11513901Reflector ICR
Fluorescence filter systems(for material numbers see modular system brochure)
11513900Analyser
11513898Filter system POL/IGS
11501233 x/y stage ERGO incl. stage bracket and condenser holder, fixed, 1 specimen
11501234 x/y stage BIO II, oil immersion, 2 specimen
11501232 x/y stage BIO I, incl. stage bracket and condenser holder, rotatable, 2 specimen
11505163Object holder ERGO,single-hand-operation,1 specimen
11505156Object holder BIO I,single-hand-operation,1 specimen
11505157Object holder BIO II,oil immersion, 2 specimen
discussion units for 3, 5, 10 viewers (for material numbers see modular system brochure)
System OverviewLeica DM4000 B/DM4000 M/DM5000 B
11 541 540F-mount
1/2 •
11 541 541F-mount
2/3 •
11 543 706C-mount
1/2 •
11 543 702B-mount
1/2 •Sony
11 541 539B-mount
2/3 •Sony
1x1x 1x1.25x 1.25x
MFK2
11581081 mot. comparison bridge
11505073 discussion unit for 2 viewer
10 11
DM4000 B DM5000 B
Stand Power supply – Integrated in stand – In CTR5000 electronics box
Display – Information display (3.7x7.7 cm) – Touch Screen (7.3x7.3 cm) with information and operation fields
Transmitted light axis Illumination – 12V 100W halogen lamp – 12V 100W halogen lamp
Automation – Contrast and Light Manager (adjustment of light intensity, field and aperture diaphragm)– Constant Color Intensity Control (CCIC)
Contrast techniques – BF (brightfield) – BF (brightfield)– PH (phase contrast) – PH (phase contrast)– DF (darkfield) – DF (darkfield)– POL (polarisation) – POL (polarisation)
– ICT (interference contrast fortransmitted light)
Fluorescence axis Illumination – 100W Hg lamp; 50W Hg lamp – 100W Hg lamp; 50W Hg lamp
Automation – Contrast and Light Manager (adjustment of light intensity and field diaphragms)– FIM (fluorescence intensity management) technique for reducing the light intensity in 5 steps– Booster lens for increasing the light intensity (optional)– Circular and rectangular field diaphragms for eyepiece or camera viewing
Mot. filter turret – 5-position – 5-position– 8-position
Condensers Automation – Mot. condenser top – Mot. condenser top– Mot. condenser turret (7 pos.) optional – Mot. condenser turret (7 pos.) optional
– Mot. polariser optional
Objective nosepiece – 6-position M25, coded – 7-position M25, coded
Stages – Ceramic-coated – Ceramic-coated– Without rack on y drive – Without rack on y drive– Adjustable torque – Adjustable torque– Telescopic stage drive – Telescopic stage drive– With and without 110° rotation – With and without 110° rotation– Left-hand version on request – Left-hand version on request
Technical Data
In my biochemistry course about 25 years ago I used to study moleculesby the diagram of biochemical paths. Today, I can watch some of theseold acquaintances at work and see them wandering from one living cell tothe next and influencing cellular functions. Labelling molecules is now nolonger a problem – using a research or confocal microscope of the LeicaFluorescence Microscopy range. Leica also offers me top-precisionimage analysis systems for their subsequent quantification.
Prof. Dr. H.J. Tanke, Leiden University Medical Centre
“Labelling molecules was as hard aswriting on grains of dust with a pencil –until recently.”
Leica Microsystems –the brand for outstanding products
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Leica Microsystems’ mission is to be the world’s first-choice provider of innovativesolutions to our customers’ needs for vision, measurement, lithography and analysisof microstructures.
Leica, the leading brand for microscopes and scientific instruments, developed fromfive brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and CambridgeInstruments. Yet Leica symbolizes innovation as well as tradition.
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