Leica AM6000Simply Microscopy!

The New Standardfor Micromanipulation

IntelligenceBrillianceConvenienceIntegration

Leica Microsystems and Eppendorf present the second genera-tion of their successful micromanipulator series. As the suc-cessor to the Leica AS TP Transgenic Platform, the AM6000Advanced Micromanipulation system is based on Leica’s latestdevelopment – the Leica DMI 6000 B inverted microscope. Usersof the AM6000 will benefit from all of the advantages of this fullyautomated research microscope and its integration with Eppen-dorf’s electrical micromanipulators.

Intelligent system operation The intelligence of the Leica AM6000 is impressive. The manipu-lation system lets you observe and manipulate specimens simul-taneously. Using the contrast or illumination manager, the AM6000provides razor-sharp, brilliant images. And the Eppendorf manipu-lators feature many useful functions to facilitate your work.

Experience and innovation–the art of creating brilliant imagesThe transmitted light axis supports all transmitted light methods –and more. Let the integrated Leica interpupillary interface and itsunparalleled possibilities surprise you. The new fluorescenceaxis offers all of the brilliance, depth of field, and resolution thatyou expect from a light microscope.

Experts call it ergonomics. We call it convenience.A lot has been written about ergonomics. Discover the conveni-ence of ergonomics with the Leica AM6000. Leica and Eppendorfdevelopers designed an easy-to-use system that not onlyexceeds the latest technical standards, but more than fulfills allergonomic requirements.

A team is more powerful than the sum of its playersLeica products are team players that are carefully in tune withone another and build on each other’s strengths. The AM6000 wasdeveloped in cooperation with Eppendorf engineers. The consist-ent integration of microscope and micromanipulators in the newmanipulation system provides a wide range of new options thatyou will soon consider essential.

The New Standardfor Micromanipulation

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“We asked many users worldwide for suggestions on how to optimize

their typical microscope-micromanipulation workflow. The main mess-

age was: ‘I would like to leave my hands on a single control unit to

operate both the microscope functions and micromanipulators’. I am

very pleased to announce that we have succeeded in converting

these suggestions into reality. We now have a unique and really inte-

grated system – Leica AM6000. See for yourself – you´ll be glad you did.”

Dr. Werner Wittke,Product Manager, Micromanipulation and Microscopy, Leica Microsystems

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The intelligent base – Leica DMI 6000 BThe inverted DMI 6000 B digital microscope leaves nothing to bedesired. The entire instrument is fully automated so that the usercan concentrate on the experiment, and not on the microscopefunctions. The contrast and illumination manager take over all ofthe work when changing contrast – at the touch of a button – intransmitted light as well as in fluorescence. For detailed informa-tion, please request the Leica DMI 4000–6000 B Series productbrochure.

The ideal partner – Eppendorf micromanipulatorsEppendorf’s electrical micromanipulators are integrated to thecomplete system. In addition to proven functions such as “Home”,“Clean”, “Y-off”, and “Limit”, other functions are available thatare unique to the AM6000. The joystick, for example, can controlthe manipulators and the motorized microscope stage. Both com-ponents feature coarse and fine modes for fast travel and work-ing with micron precision.

The key to enhanced efficiency – the multifunction panelsThe entire system is controlled from multifunction panels thatmanage the most important microscope functions in addition to thefull range of micromanipulation functions. This integrated systemis your key to accelerated work processes and higher efficiency.In addition to all NK2-generation Eppendorf manipulator func-tions, the control panels feature focus hand wheels and functionbuttons to control brightness, the objective turret, and the magni-fication changer.

Customize the AM6000 to suit your work with three freely pro-grammable function buttons. The objective, subsequent magnifi-cation, and contrast method can be stored and recalled with thetouch of a button. You can switch instantly between contrasts andmagnifications – ideal for repetitive processes and perfectlyreproducible experimental conditions!

Intelligent Imaging –Simply Press a Button

I n t e l l i g e n c e

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Clearly designed controlsAll microscope settings at a glance: The current con-trast method, selected magnification, illuminationparameters, and camera ports, as well as informationabout the focal plane, are all available. The mostimportant fluorescence functions at a glance: Eachfilter cube can be selected directly. The shutter opensor closes at the touch of a button. Immediate feed-back is displayed.

Focus on the actionThe microscope stage, objective turret, condenser,and manipulators form the heart of the Leica AM6000.They are perfectly attuned to one another for optimalsoftware-supported interaction.

Leica AM6000• Combination contrast – the simultaneous use of DIC and

fluorescence – is activated at the touch of a button andensures rapid monitoring of fluorescence probes.

• The Parfocality Manager ensures a sharp image at alltimes – even after changing the magnification.

• The micromanipulation stage with its slim design allowseasy adaptation of manipulators.

• The cooling insert provides an optimal injectionenvironment for the cells.

• The “Y-off” function of the electrical micromanipulatorsmoves the instruments only in the X direction, whichfacilitates injection.

• The “Limit” function of the manipulators sets a lower Z limit to effectively prevent broken needles.

• The multifunction panel acts as the control unit for the entire system, reducing the number of required remotecontrol units.

Eppendorf FemtoJet®

• Precisely calculated quantities of genetic material can be injected with femtoliter precision with theFemtoJet®.

Anti-vibration stage• Anti-vibration stages offer freedom from mechanical

interference. The stage is precisely tailored to thedimensions of the Leica AM6000.

Sample Application: Transgenics

Transgenics places high demands on the complete system, andthe Leica AM6000 is ideal for the application. The AM6000 inte-grated solution consists of a Leica AM6000, equipped with anEppendorf FemtoJet®, a cooling insert, and an anti-vibration stage.

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B r i l l i a n c e

Experience and Innovation –The Art of Creating Brilliant Images

Always the right contrastThe basis of the Leica AM6000 is a research microscope, so youcan use any contrast method, as detailed below.

Contrast and resolution for every specimen – the new DICIt’s a familiar phenomenon when using DIC: Improved contrastresults in lower resolution and vice versa. This effect increaseswhen observing particularly thick or thin specimens. Leica offersspecial prism combinations for these cases: prism C for regularspecimen thicknesses, C1 for especially thick, and C2 for es-pecially thin specimens.

Take the optics into your own hands – the new IMCLeica’s optics experts have created an intermediate interpupillaryinterface. Now users have an effective, yet affordable modulationcontrast solution with brightfield objectives.

Seeing with different eyes – the new fluorescence axisAlso, Leica has completely overhauled the fluorescence axis,optimizing it with regard to transmission, image flattening, andlight flux qualities. The result is a fluorescence axis of peerlessoptical quality.

The unique functions of the fully automated AM6000 fluorescenceaxis will facilitate your workflow. Imaging specimens with multiplefluorescence probes becomes easy with the ultra-fast IFW filterwheel and motorized excitation manager.

For detailed information, please see theLeica DMI 4000–6000 B Series product brochure.

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A disk like no otherThis universal genius is located in the heart of thefluorescence axis. Leica engineers have installed theFIM (fluorescence intensity manager), two fast shut-ters, a motorized excitation manager, and the IFW(internal fast filter wheel) on a 49.5 mm diameter disk.

Hard, contrast-pronounced modulation contrast

Soft modulation contrast

Photos: C. Mehnert, Center for In-Vitro Fertilization,Giessen, Germany

Leica AM6000• IMC – integrated modulation contrast – provides a

relief-like image and works perfectly with plasticPetri dishes – an absolute must in human reproductivemedicine.

• A polarizer allows a brightfield share to be mixedinto the IMC image to vary the impression.

• The Parfocality Manager ensures a sharp image atall times – even after changing the magnification.

• The micromanipulation stage with its slim designallows easy adaptation of manipulators.

• Heating inserts guarantee optimal experimentalconditions for the cells.

• The Y-off function of the electrical micromanipulatorsmoves the instruments only in the X direction,which facilitates injection.

• The Limit function of the manipulators sets a lower Z Limit to effectively prevent broken needles.

• The multifunction panel acts as the control unit for theentire system, reducing the number of required remotecontrol units.

Leica DFC320• The high-resolution DFC320 Digital Camera features a

FireWire interface for rapid image transfers for a fastlive image.

Accurate Sperm Viewing• A continuous zoom applied to the camera allows sperm

to be magnified to over 10,000x. This opens new possi-bilities for judging the quality of sperm.

Sample Application: ICSI

An ICSI system combines ease of use with the option of project-ing images onto a monitor for educational purposes. The systemshown below meets these requirements.

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Photos: Pr. Stéphane Viville, Service de Biologie de la Reproduction, Schiltigheim, France

C o m f o r t

Convenient insightsThe ergonomic tube is a standard feature of the AM6000. It isincluded in the total system price, and costs no more than a fixedtube. The viewing angle of the tube can be adjusted continuouslyto ensure the best possible user posture.

Convenient overviewsThere is no substitute for an unobstructed clear view of the spe-cimen. That’s why Leica invented the “viewing channel” – a notchbetween the eyepieces that provides a clear view of the speci-men regardless of the position of the ergonomic tube.

Convenient operationThe complete system can be controlled from the multifunctionpanel, designed by Leica in cooperation with Eppendorf engin-eers. It not only controls the manipulators, but also the micro-scope. The entire system can be controlled remotely – with a singlehand.

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The Experts Call It Ergonomics -We Call It Convenience

Integrated ergonomic tubeEvery Leica DMI 6000 B is equipped with an ergonomictube. The continuous adjustment of the eyepiecesensures optimal viewing angles for all users. The“viewing channel” provides an unobstructed view ofthe specimen at all times.

Unobstructed view of the specimenThe “viewing channel” integrated in the tube provides a clear view of your speci-men, and lets you switch from microscopic to visual observation of your specimensat any time without changing the tube setting.

Multifunction panelThe multifunction panel controls manipulator andmicroscope functions such as focus, objective selec-tion, and light intensity. As another special conveni-ence, the magnification and contrast can be simul-taneously stored and recalled on three freelyprogrammable buttons.

“I have always been fascinated by the unknown and

the best way to satisfy my curiosity is research.”

Pr. Stéphane Viville, Institut de Génétique et de Biologie,Moléculaire et Cellulaire, Illkirch, France andService de Biologie de la Reproduction SIHCUS-CMCO,Unité fonctionnelle de Diagnostic Preimplantatoire, Schiltigheim, France

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SpecificationsPower supply for manipulatorSupply voltage:

Input: 120–240 V ACOutput: 15–18 V DC

Frequency:Input: 50–60 Hz

Power consumption:Input: < 50 WOutput: 25 W

Control panelDimensions: 270 x 205 x 160 mmSupply voltage: 15–18V DCPower consumption: 25 WAmbient temperature: max. 40 °CRelative humidity: max. 80 % up to 30 °COvervoltage category: IIDegree of contamination: 2

Manipulator moduleDimensions of Y/Z module: 120 x 100 x 68 mmDimensions of X module: 120 x 60 x 50 mmMaximum travel per axis: > 22 mmMicrostep resolution: approx. 40 nmMaximum speed of tool: 7,500 µm/s

WeightsMicroscope: 53 kgMotorized cross-stage: 2.8 kgControl panel with power supply: 2.7 kgModule unit, including guide: 1.4 kgComplete system: 62 kg

The system bears the mark.Technical specifications subject to change.

623 mm

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265

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126 mm

268 mm 151 mm

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Stand

Focus

Objective turret

Controls

Stages

Transmitted-light axes

Condensers

Fluorescence axis

Automation

Power supply

Display

Interfaces

General

Stand

SmartMove (remote control)

Mechanical stages

Motorized stages

Illumination arm

General

S1–28

S70

Filter disk

Illumination

Focus

Illumination Manager, transmitted light

CCIC

Contrast Manager

Illumination Manager, fluorescence

Leica DMI 6000 B• in CTR6000 electronics box

• information display 77 x 49 cm (W/H)

• RS232• ready for USB

• motorized• 5 electronic transmissions• switching between coarse and fine mode

• 6x M25• motorized, absolutely coded

• 7 freely programmable buttons• buttons with fixed functions for

– illumination manager, focus functions, camera ports,subsequent magnification, fluorescence functions

• control element for focus (z) and stage movement (x, y)• 4 freely programmable buttons

• fixed stages– ceramic-coated– over 20 different inserts available– can be equipped with heating and cooling inserts

• 3-plate cross-stages (also slim form for micromanipulator)

• 12 V/100 W halogen lamp• motorized field diaphragm (mechanical optional)• filter magazine for 2 filters, motorized (mechanical optional)• motorized shutter (mechanical optional)

• condenser disk for optical elements with– 4 large openings for prisms, DF stop, BF, PH rings– 3 small openings for BF, PH rings

• integrated motorized aperture diaphragm (mechanical optional)• suitable for magnifications from 1.25x to 100x• separate polarizer (optional; mechanical or motorized)

• motorized condenser disk (coded optional)• motorized condenser head (mechanical optional)

• motorized condenser disk (coded optional)• fixed condenser head • mechanical lens for low magnifications

• motorized• for up to 6 filter cubes

• 100 W hg lamp

• including parfocality function• positioning memory slots for fast traversal to 2 z-positions

• adjustment of brightness, aperture and field diaphragmsto the objective and contrast technique currently in use

• Constant Color Intensity Control – maintains color temperature of 3200 K

• adaptation of optical elements such as prisms or light rings to the objective and contrast technique currently in use

• FIM (Fluorescence Intensity Manager) – adaptationof brightness in 5 fixed levels

• adjustment of field diaphragm to the eyepieces or camera chips (round or rectangular diaphragms)

• IFW (Integrated Fast Filter Wheel) – very fast switching of excitation withchangeover times of less than 0.05 second

• Leica Excitation Manager – red-green attenuation in 8 levels

F l e x i b i l i t y

The best stageThe Leica AM6000 supports a large selection of specimen stages,and you can choose among fixed and 3-plate cross-stages.Motorized stages may also be used. A special new developmentfor the AM6000: an extra-slim motorized 3-plate stage for micro-manipulation.

Contrast for every applicationA broad selection of condensers is available for the AM6000. From1 to 70 mm, any working distance is possible. Leica’s S1 to S28condensers (1–28 mm working distance) are universal conden-sers for magnifications from 1.25x to 100x.

From refrigerator to saunaStage inserts for any type of container and temperature controlunits provide the correct temperature for your experiments. Frombelow freezing to +60°C, any temperature is possible – the choiceis yours.

Well-Adjusted –and Yet Individual

The new condenser generationA new generation of manual/coded and motorizedcondensers is available for the DMI Series. Thisrange of condensers is the first ever to support mag-nifications of 1.25x to 100x in inverted microscopes.Another new feature is the plug connection betweenthe condenser and illumination arm, which eliminatescables and makes condenser replacement easy. Andthanks to the Koehler locking lever, users can storetheir optimal Koehler settings.

The aperture diaphragm, integrated in all conden-sers, is available in manual and motorized versionsfor optimal support of the Illumination Manager. Allcondensers feature septuple condenser disks, mak-ing them suitable for all contrast methods.

Leica EL6000New external light source for fluorescence excitation.The adjustment-free metal-halogenide lamp with itslong bulb life saves time, money and energy.

The narrow stage for micromanipulationThis stage, which was developed in cooperation with a leading manufacturer ofmicroscope stages, is designed to leave as much room on either side of the micro-scope as possible – space that is available to micromanipulation systems, andwhich eliminates the risk of collisions with the motor.

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Leica Microsystems –the brand for outstanding products

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.Leica Microsystems’ mission is to be the world’s first-choice provider of innovativesolutions to our customers’ needs for vision, measurement, lithography and analysisof microstructures.

Leica, the leading brand for microscopes and scientific instruments, developed fromfive brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and CambridgeInstruments. Yet Leica symbolizes innovation as well as tradition.

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and representatives of Leica Microsystemsin more than 100 countries.

The companies of the Leica MicrosystemsGroup operate internationally in four businesssegments, where we rank with the marketleaders.

• Microscopy SystemsOur expertise in microscopy is the basis for allour solutions for visualization, measurementand analysis of microstructures in life sciencesand industry. With confocal laser technologyand image analysis systems, we provide three-dimensional viewing facilities and offer newsolutions for cytogenetics, pathology and mate-rials sciences.

• Specimen PreparationWe provide comprehensive systems and ser-vices for clinical histo- and cytopathologyapplications, biomedical research and indus-trial quality assurance. Our product rangeincludes instruments, systems and consum-ables for tissue infiltration and embedding,microtomes and cryostats as well as automat-ed stainers and coverslippers.

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