Lec02.Safety

8/19/2019 Lec02.Safety

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AFETY IN THE

LABORATORY


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The most common seriousinjuries: cuts due to carelessuse of razor blades

Example: Carpal Tunnel Syndrome from writing lab reports

RE EARCH EMPLOYEEINJURIE , 1993 - 1997*


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LABORATORY SAFETY

CHEMICAL SAFETYPERSONEL PROTECTION

SAFE OPERATION OF EQUIPMENTFIRE AND ELECTRICAL SAFETY

http://www.ehs.ufl.e u

http://www.ehs.ufl.edu/http://www.ehs.ufl.edu/


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MATERIAL AFETY DATA HEET

! P"ep#"e $% &ts '#(uf#)tu"e"* #( MSDS p"+,& es&(f+"'#t&+( t+ help %+u u( e"st#( the &(t"&(s&) h#-#" s+f the )he'&)#l &()lu &( :

!Ph%s&)#l #( )he'&)#l p"+pe"t&es!St#$&l&t% #( "e#)t&,&t% &(f+"'#t&+(!He#lth h#-#" &(f+"'#t&+(! A)ute #( )h"+(&) effe)ts +f e p+su"e!Pe"'&ss&$le e p+su"e l&'&ts

MATERIAL SAFETY DATA SHEETS

SHOULD 0E STORED IN THE LA0

1HERE YOU ARE 1OR2IN3

YOU SHOULD TA2E THE TIME TO 0ECOME

FAMILIAR 1ITH THE HA4ARDS OF ANY

CHEMICAL YOU ARE 1OR2IN3 1ITH

http://www.fishersci.com/wps/portal/CMSTATIC?pagename=msds


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CHEMALERT

FISHER LABEL

http://www.ilpi.com/msds/ref/nfpa.html


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TOXICITY

ACUTE 5 se,e"e #( +f sh+"t u"#t&+(CHRONIC 5 )+( &t&+( th#t l#sts +,e" # l+(pe"&+

DERMAL E6POSURERe'e'$e" )he'&)#ls su)h #s 'eth#(+l #"e

t+ &) #( )#( $e #$s+"$e th"+u h the s7&(.INHALATION

M#(% s+l,e(ts use &( )h"+'#t+ "#ph% #"ee t"e'el% ,+l#t&le #( t+ &). Use the fu'e h++ 8


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FLAMMABILTY

N+te: D+(9t t"% th&s #t h+'e8O" &( the l#$;.

MANY CHEMICALS SUCH AS METHANOL*

ACETONITRILE* < TETRAHYDROFURAN

THAT ARE USED IN CHROMATO3RAPHY

ARE E6TREMELY FLAMMA0LE8


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LABORATORY SAFETY PRACTICES

N+ E#t&( +" D"&(7&(

C+(t#)t Le(sesSh+ul N+t 0e 1+"(&( the L#$N+ Ope(5T+e Sh+es+" S#( #ls #"e

All+we


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CHEMICAL SAFETY

CHEMICAL STORA3ESPILL CONTROL1ASTE DISPOSAL


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OF PARTICULAR CONCERN ARE

ETHER AND TETRAHYDROFURAN

1HICH CAN FORM E6PLOSI=E

PERO6IDES O=ER TIME.

CHEMICAL STORAGE


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!IF THERE IS A NEED TO REFRI3ERATE A FLAMMA0LE SU0STANCE

IT MUST 0E STORED IN A U.L. LISTED OR F.M APPRO=ED

E6PLOSION PROOF5REFRI3ERATOR

!SUCH REFRI3ERATORS OR FREE4ERS ARE DESI3NED SO

THAT FLAMMA0LE =APORS DO NOT CONTACT SPAR2S

FREEZER EXPLOSION

Pet"+leu' ethe" w#s st+"e &( #( +" &(#"% +'est&) f"ee-e"O,e" t&'e the ethe" e,#p+"#teThe )+()e(t"#t&+( e )ee e the l+w e pl+s&,e l&'&t* #$+ut>.?@

A sp#"7 f"+' #( &(te"(#l )+'p+(e(t )#use the e pl+s&+(


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Absorbant Materials

Chemical Neutralizing AgentsPlastic Waste

Disposal Bags

SPILL CONTROL


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MA2E SURE YOU 2NO1 HO1 TO

SAFELY DISPOSE OF CHEMICALSUSED DURIN3 LA0 E6ERCISES

CHEMICAL WASTE DISPOSAL


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F&"st A& 2&ts Sh+ul $e "e# &l% #))ess&$le

&( #ll L#$+"#t+"&es.

Is &t F&"st A&

+" F&"st A& e

FIRST AID


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PRE URIZED GA E

USE 3AS CYLINDERS IN 1ELL

=ENTILATED AREAS ONLY

SECURE ALL CYLINDERS0Y A CHAIN OR STRAP


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PRESSURIZED GASSES

REPORT/REPLACE ALL CYLINDERS

IN QUESTIONA0LE CONDITION

MA2E SURE HYDROSTATIC

TESTS ARE CURRENT


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PRESSURIZED GASSES

LEA2 TEST FITTIN3S* PIPIN3* AND

CONNECTIONS 0EFORE 1OR2 0E3INS

RELEASE PRESSURE AND CLOSE =AL=E

AT THE END OF THE DAY

NOT HEREHERE


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PRESSURIZED GASSES

REPLACE CAPS 1HEN

CYLINDERS ARE NOT IN USE

STORE IN COOL* 1ELL =ENTILATED

AND PROTECTED AREA


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PRESSURIZED GASSES

MO=E CYLINDERSONLY ON A HANDTRUC2 OR OTHERCART DESI3NEDFOR SUCHPURPOSEMA2E SURE THECAP IS ON AND THETAN2 IS STRAPPEDTO THE CART


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E=ERY DAY AN ESTIMATED

>*??? EYE INBURIES

OCCUR IN AMERICAN 1OR2PLACES

EYE PROTECTION


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POTENTIAL EYE AND FACEHAZARDS

Chemical SplashesDustSmoke and umes

!elding "perations#asers$"ptical %adiation&ioaerosols

'rojectilesULTRA=IOLET LI3HT FROMSPECTROPHOTOMETERS ARE APOTENTIAL HA4ARD. MA2ESURE YOU ARE 1EARIN3 SAFETY 3LASSES

1ITH U= PROTECTION 1HEN CHAN3IN3 0UL0S.


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DISPOSA0LE APRONS 1ILL 0E

A=AILA0LE FOR YOU TO USE

DURIN3 LA0 E6PERIMENTS

LAB COATS


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USE 3LO=ES THAT ARE

SUITA0LE FOR THE BO0.

GLO ES


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SAFE OPERATION OFE!UIPMENT

#(& T""#S)*E +""DS

()T"C#(,ESCE-T%. )/ESC+%"*(T"/%('+S


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SAFE HANDLING OF LAB TOOLS

0"+7e( l#ss &s sep#"#te f"+' "e ul#"

w#ste st"e#' #( p"+pe"l% l#$ele .

Nee les #( s'#ll sh#"ps #"e &sp+se

+f &( "e pl#st&) sh#"ps9 $+ .


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MA2E SURE IT9S

TURNED ON8

LA0EL ALL CHEMICALS

STORED IN THE HOOD

SAFE USE OF FUME HOODS


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1OR2 AT THE CORRECT

SASH HEI3HT

1OR2 AT LEAST INCHES

FROM THE FRONT OF THE HOOD



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DO NOT PLACE ELECTRICAL APPARATUS

OR OTHER I3NITION SOURCES INSIDE

THE FUME HOOD IF FLAMMA0LE LIQUIDS

OR 3ASSES ARE PRESENT.



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1HAT HAPPENS IF THE PO1ER 3OES OUTSAFE USE OF FUME HOODS


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SAFE USE OF AUTOCLA ES

AUTOCLA=E E6PLOSION

THE CAUSE 1AS NOT

DETERMINED

E pl+ &( Aut+)l#,es* NOT # ++ th&(


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DON9T PLACE FLAMMA0LE ITEMS

SUCH AS PAPER TO1ELS

NEAR THE AUTOCLA=E8

DON9T AUTOCLA=E

FLAMMA0LE OR

=OLATILE LIQUIDS


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USE METAL TU0S TO HOLD SAMPLES

NAL3ENE TU0S MAY 3ET

LESS STURDY AS THEY A3E AND MAY SOFTEN

1HEN E6POSED TO

HEAT. THE TU0S MAY

0UC2LE* SPILLIN3 THEIRHOT CONTENTS.


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0EST SAFETY IDEA:

CLOSE THE DOOR AND RUN8


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The )#use +f the #))& e(t &s $el&e,e t+ $e the use

+f # '+ el +f "+t+" th#t

w#s (+t #pp"+,e $%

0e)7'#( f+" use &( #'+ el LG5 0 ult"#)e(t"&fu e.

CENTRIFU3E

E6PLOSION8

SAFE USE OF CENTRIFUGES


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The h#lf5&()h th&)7 sl& &( steel++" +( t+p +f the u(&t $u)7le

#ll+w&( f"# 'e(ts* &()lu &( thesteel "+t+" t+p t+ es)#pe.F"# 'e(ts "u&(e # (e#"$%"ef"& e"#t+" #( #( ult"#5)+lf"ee-e"* '# e h+les &( the w#lls#( )e&l&( * #'# e )#$&(ets #(shel,&( th#t )+(t#&(e +,e" #hu( "e )+(t#&(e"s +f )he'&)#ls*

#( sh#tte"e #ll f+u" w&( +ws &(the "++'. F+"tu(#tel% the "++'w#s (+t +))up&e #t the t&'e #(the"e we"e (+ pe"s+(#l &( u"&es.



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! %E(D T+E S( ET0 *(-)E#

! .## ")T )SE #"/

! D"-1T #E(,E T+ECE-T%. )/E )-T.# )##"'E%(T.-/ S'EED .S"&T(.-ED

! . ,.&%(T."- "CC)%S2 ST"'T+E %)- .**ED.(TE#0


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USE ONLY ROTORS DESI3NED

FOR THE INSTRUMENT

NE=ER E6CEED THE MA6IMUM

RECOMMENDED SPEED OF THE ROTOR


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NE=ER OPERATE A CENTRIFU3E

1ITHOUT THE LID OR CO=ER

LOC2ED IN PLACE


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.

.

ALL SAMPLESSHOULD 0ECAPPEDUSE TU0ESDESI3NED FORTHE PARTICULARROTORCLEAN UP SPILLSPLASTIC TU0ESSHOULD NOT 0EREUSED AS THEIRFAILURE RATEINCREASES


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.

MOSTIMPORTANTLY:

MA2E SURE THETU0ES ARE

SYMMETRICALLYLOADED ANDCAREFULLY0ALANCED

"#' #t > 3 &s"+u hl% e u&,#le(t t+G ? 2 J ??*???3Ks


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THIS CAPILLARY ELECTROPHORESIS

SYSTEM OPERATES AT O=ER G?*??? =OLTS.

ELECTRICAL SAFETY


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RE=IE1 THE PROCEDURES

FOR THE USE OF A

FIRE E6TIN3UISHER

FIRE SAFETY


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DON9T MA2E A MESS8

CLEAN UP AFTER YOURSELF8

Lec02.Safety

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