Leafy Vegetables - projects.cgn.wur.nl Aarau WP2.pdf · Leafy Vegetables Characterization 797 112...
Transcript of Leafy Vegetables - projects.cgn.wur.nl Aarau WP2.pdf · Leafy Vegetables Characterization 797 112...
Leafy Vegetables
WP2: Characterisation and Regeneration
Ulrike Lohwasser, IPK Gatersleben, Germany
Characterization
Descriptors
Regeneration
WP2 regeneration & characterization
Year 4
Year 3
Year 2
Year 1
WP activities
Leafy Vegetables
128314646182112797Characterization
1254334020149931Regeneration
TotalRucolaValeria-nella
ChicorySpinachLettuce
Fourth Year: Milestones & Deliverables
WP2: Characterisation and Regeneration
36
Regeneration and characterisation for all crops started
12
D07- D11
D06
Deliverable #
Start made developing minimum descriptors for the minor leafy vegetables
02
Material for regeneration and characterisation of the four crops selected
06
Minimum descriptors for the minor leafy vegetables concluded
06
Regeneration of some crops started pending the growing season
06
75% of all accessions characterised34
All accessions characterised45
M&D descriptionMonth
75% of all accessions regenerated
D12- D15 All accessions regenerated48
D1348
Characterization data entered in a fixed format to be sent to the database managers
D1145
D14
D12
D10
D09
D08
D07
Deliverable #
110 accessions of spinach characterized45
180 accessions of chicory characterized45
800 accessions of lettuce characterized45
190 accessions of minor crops characterized45
930 accessions of lettuce regenerated48
200 accessions of chicory regenerated48
M&D descriptionMonth
50 accessions of spinach regenerated
D15 70 accessions of minor crops regenerated48
Fourth Year: Milestones & Deliverables
WP2: Characterisation and Regeneration
Lettuce Spinach ChicoryValeria-
nella RucolaMonth 48 48 48 48 48 48 48 48 48 48
P0 CGN 70 60 29 15 - - - - 11 5P1 HRI 105 60 15 15 18 18 - - 4 4P2 GEVES 8 8 - - 81 60 6 8 - -P3 IPK 330 320 - - 101 100 16 15 28 24P4 Palacky - - - - - - - - - -P5 NordGen 6 6 12 7 - - 1 0 - -P6 INRA 300 300 - - - - - - - -P7 Arche Noah 61 60 12 12 16 16 8 8 - -P8 HDRA 17 17 - - - - - - - -P9 UNIBO - - - - - - - - - -P10 KIS 112 100 - - 7 7 7 9 - -P11 ProspecieRara - - - - - - - - - -
∑ 1009 931 68 49 223 201 38 40 43 33
Regeneration
Lettuce Spinach ChicoryValeria-
nella RucolaMonth 48 48 48 48 48 48 48 48 48 48
P0 CGN 765 60 - - - - - - 17 5P1 HRI 54 30 30 30 - - - - - -P2 GEVES - - - - 97 75 - - - -P3 IPK 514 500 - - - - 35 37 151 141P4 Palacky 30 30 - - - - - - - -
P5 NordGen - - 70 70 - -19 Atri - - -
P6 INRA - - - - - - - - - -P7 Arche Noah 61 60 12 12 - - - - - -P8 HDRA 17 17 - - - - - - - -P9 UNIBO - - - - - - - - - -P10 KIS 112 100 - - 8 7 7 9 - -P11 ProspecieRara - - - - 109 100 - - - -
∑ 1553 797 112 112 214 182 42 46 168 146
Characterisation
Characterisation and Regeneration (total numbers)
108%
195%
139%
100% 111%118%
95% 91%
130%115%
0%20%40%60%80%
100%120%140%160%180%200%
Lettu
ceR
egen
erat
ion
Lettu
ceC
hara
cter
isat
ion
Spi
nach
Reg
ener
atio
n
Spi
nach
Cha
ract
eris
atio
n
Chi
cory
Reg
ener
atio
n
Chi
cory
Cha
ract
eris
atio
n
Val
eria
nella
Reg
ener
atio
n
Val
eria
nella
Cha
ract
eris
atio
n
Ruc
ola
Reg
ener
atio
n
Ruc
ola
Cha
ract
eris
atio
n
Characterisation
Descriptors available for:- Cultivated Lettuce- Endive- Eruca- Leaf Chicory- Root Chicory- Spinach- Valerianella- Wild Lactuca
Partner Characterisation Data AvailabilityP0 CGN for lettuce, rucola yesP1 HRI for lettuce, spinach yesP2 GEVES for chicory yesP3 IPK for lettuce, Valerianella, rucola yesP4 Palacky for lettuce yesP5 NordGen for spinach yesP6 INRA not necessary -P7 Arche Noah for lettuce, spinach yesP8 HDRA for lettuce yesP9 UNIBO not necessary -P10 KIS for lettuce, chicory, Valerianella yesP11 ProspecieRara for chicory yes
Availability of characterisation data
Quality of characterisation data
Partner Characterisation Data QualityP0 CGN for lettuce, rucola excellentP1 HRI for lettuce, spinach excellentP2 GEVES for chicory excellent
P3 IPKfor lettuce, Valerianella, rucola excellent
P4 Palacky for lettuce excellentP5 NordGen for spinach excellentP6 INRA not necessary -P7 Arche Noah for lettuce, spinach excellentP8 HDRA for lettuce excellentP9 UNIBO not necessary -P10 KIS for lettuce, chicory,
Valerianella not as requestedP11 ProspecieRara for chicory excellent
Publication in professional paperin press 2010
Ein europäisches Projekt beschäftigt sich mit Blattgemüsen –12 Institutionen aus 10 Ländern sind beteiligt
S. Thumm, M.-L. Graichen, A. Börner & U. Lohwasser
Gemüse – das Magazin für den professionellen Gemüsebau
Gap Analysis of Eruca and DiplotaxisGenpool Concept
Genpool IBiological Species:
cultivated and spontaneous
races
Source: Harlan & De Wet, 1971
GP II GP II
All species that canbe crossed with GP I
Gene transfer possiblebut may be difficult
GP III GP III
Hybrids with GP Ianomalous, lethal or sterile
Gene transfer not possible orrequiring radical techniques
Gene pool Species Author Database
Primary Eruca sativa Mill. 495
Primary Eruca vesicaria (L.) Cav. 171
Primary Eruca pinnatifida(Desf.) Pomel 13
Secondary Diplotaxis tenuifolia (L.) DC. 49
Secondary Brassica oleracea L.
Secondary Brassica repanda (Willd.) DC.
Tertiary Brassica oleracea L.
Tertiary Brassica rapa L.
Tertiary Brassica juncea L.
Total 728
Genepool of Eruca
Reg
ion
Eruc
asa
tiva
Eru
cave
sica
ria
Eruc
api
nnat
ifida
Dip
lota
xis
tenu
ifolia
Bra
ssic
aol
erac
ea
Bras
sica
repa
nda
Bra
ssic
aol
erac
ea
Bras
sica
rapa
Bras
sica
junc
ea
Eru
cast
rum
variu
m
Eruc
astru
mca
narie
nse
Eru
cast
rum
card
amin
oide
s
Europe 144 32East 3North 1Middle 17 5 2South 106 106 28 1 4Southeast 5 1 1West 13 1Asia 291 4Central 12East 6Near East 27 2 4South 234 5West 12 4Africa 29East 1North 28 17 8 31SouthAmerica 6 5North 6 3 3South 1 2
unknown 25 26 2 7 2
Genepool of Eruca
Eruca
Secondary genepool (Diplotaxis tenuifolia, Brassica repanda, B. oleracea)
Primary Genepool (natural distribution, naturalized elsewhere, except tropics)
Tertiary genepool (B. oleracea Europe, B. junceaEast Asia, B. rapa widely distributed)
B. junceaB. oleracea
Eruca
Primary genepoolSecondary genepool
Diplotaxistenuifolia
Gene pool Species Authority Database
Primary Diplotaxis tenuifolia (L.) DC. 49
Secondary Brassica rapa L.
Secondary Brassica juncea (L.) Czern.
Secondary Brassica nigra (L.) W. D. J. Koch
Tertiary Erucastrum virgatum C. Presl 38
Tertiary Brassica oleracea L.
Tertiary Brassica elongata Ehrh.
Total 87
Genepool of Diplotaxis tenuifolia
Genepool of Diplotaxis muralis
Diplotaxismuralis
Gene pool Species Author Database
Primary Diplotaxis muralis (L.) DC. 49
Secondary Brassica juncea L.
Secondary Brassica napus L.
Secondary Brassica rapa L.
Tertiary Diplotaxis harra (Forssk.) Boiss. 31
Tertiary Erucastrum gallicum(Willd.) O. E. Schulz
Total 80