LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP...

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lacZ lacY lacA O A i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operato r activator Regulatory gene P lacZ lacY lacA O A i NO transcription lacZ lacY lacA O A i NO transcriptio n lacZ lacY lacA O A i Transcription occurs lacZ lacY lacA O A i Lac operon

Transcript of LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP...

Page 1: LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operator.

lacZ lacY lacAOAi

Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

complex CAP complex

B-galactosidase

lactose permease

transcetylase

operatoractivatorRegulatory gene

P

lacZ lacY lacAOAi

NO transcription

lacZ lacY lacAOAi

NO transcription

lacZ lacY lacAOAi

Transcription occurs

lacZ lacY lacAOAi

Lac operon

Page 2: LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operator.

Not taken up

Lots of glucose

Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

complex CAP complex

IIC

glycolysis

Glucose

PEP

Pyruvate

Kreb’s

IIB

HPr

IIA

EI ATP

glucose

glucose

lactose

Low cAMP

Page 3: LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operator.

Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

complex CAP complex

IIC

glycolysis

Glucose

PEP

Pyruvate

Kreb’s

HPr

IIA

EI ATP

IIB

lactose

Adenylate cyclase

Not more glucose

This process slows down

Levels of cAMP increase

Page 4: LacZ lacY lacA OA i Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP complex CAP complex B-galactosidase lactose permease transcetylase operator.

Repressor lactose lactose/repressor RNA pol CRP cAMP CRP/cAMP

complex CAP complex

IIC

glycolysis

Glucose

PEP

Pyruvate

Kreb’s

HPr

IIA

EI ATP

IIB

lactose

Adenylate cyclase

Not more glucose

This process slows down

lacOp becomes active


Lactose Intolerance Quick Test CLINICAL TRIAL · amount of lactose consumed; most lactose-intolerant people can tolerate a certain level of lactose in their diet without ill-effect

Lactose Intolerance Quick Test CLINICAL TRIAL · amount of lactose consumed; most lactose-intolerant people can tolerate a certain level of lactose in their diet without ill-effect

Beginning of the chapter Lactose intolerance and genetics (Lactose Sensor) 47.

Beginning of the chapter Lactose intolerance and genetics (Lactose Sensor) 47.

206 crp lowering or crp guided trial

206 crp lowering or crp guided trial

Milk Allergy & Lactose Intolerance · Lactose intolerance •A lactose intolerance is the inability to digest the milk sugar (lactose) due a lactase deficiency. •Symptoms include:--

Milk Allergy & Lactose Intolerance · Lactose intolerance •A lactose intolerance is the inability to digest the milk sugar (lactose) due a lactase deficiency. •Symptoms include:--

Chap. 7 Problem 1 In glucose media without lactose, the lac repressor is bound to the lac operator, and the CAP protein is not bound to its control site.

Chap. 7 Problem 1 In glucose media without lactose, the lac repressor is bound to the lac operator, and the CAP protein is not bound to its control site.

Case No: CRP 196/2015 - Gauhati High Courtghconline.gov.in/Judgment/CRP1962015.pdf · CRP 196/2015 CRP 263/2015 CRP 264/2015 CRP 265/2015 CRP 266/2015 . 10,75,049.82 along with interest

Case No: CRP 196/2015 - Gauhati High Courtghconline.gov.in/Judgment/CRP1962015.pdf · CRP 196/2015 CRP 263/2015 CRP 264/2015 CRP 265/2015 CRP 266/2015 . 10,75,049.82 along with interest

Thermal analysis of amorphous lactose and -lactose monohydrate

Thermal analysis of amorphous lactose and -lactose monohydrate

Prokaryotic Gene RegulationTrp Operon •Tryptophan Absent •Repressor Inactive •Operon ON. Trp Operon •Tryptophan Present •Repressor Active •Tryptophan = Corepressor •Operon

Prokaryotic Gene RegulationTrp Operon •Tryptophan Absent •Repressor Inactive •Operon ON. Trp Operon •Tryptophan Present •Repressor Active •Tryptophan = Corepressor •Operon

Plasmodium transcription repressor AP2-O3

Plasmodium transcription repressor AP2-O3

Redox -responsive repressor Rex modulates alcohol ...

Redox -responsive repressor Rex modulates alcohol ...

1. LIQUID FEEDS: REDUCED LACTOSE VS. REGULAR LACTOSE 1.1 DURATION

1. LIQUID FEEDS: REDUCED LACTOSE VS. REGULAR LACTOSE 1.1 DURATION

Gel at pH repressor-DNA

Gel at pH repressor-DNA

Wilson Farm - s3.amazonaws.coms3.amazonaws.com/loa.data/inv/3900992/wilson_5.3.17.pdfWilson Farm. H. unting | R. ecReational | i. ncome. P. Roducing. ... 3 . 1 2: CRP CRP CRP CRP CRP:

Wilson Farm - s3.amazonaws.coms3.amazonaws.com/loa.data/inv/3900992/wilson_5.3.17.pdfWilson Farm. H. unting | R. ecReational | i. ncome. P. Roducing. ... 3 . 1 2: CRP CRP CRP CRP CRP:

Chapter 18 - Biolympiads...Enzymes •Digest Lactose. •When enough Lactose is digested, the Repressor can bind to the operator and switch the Operon "off”. Positive Gene Regulation

Chapter 18 - Biolympiads...Enzymes •Digest Lactose. •When enough Lactose is digested, the Repressor can bind to the operator and switch the Operon "off”. Positive Gene Regulation

SNPing Lactose

SNPing Lactose

DNA lacZ lacI O lacY lacA C E P rep T CAP repressor CAP site pol-binding site operator structural genes terminator lac operon lactose cAMP 8.

DNA lacZ lacI O lacY lacA C E P rep T CAP repressor CAP site pol-binding site operator structural genes terminator lac operon lactose cAMP 8.

Identification Tetracycline Resistance Promoter Repressor ...

Identification Tetracycline Resistance Promoter Repressor ...

1321355349.pdfactinowcete isolation agar ... casein digest cellobiose cetrimide agar base ... lactos lactose lactose lactose lactose broth broth

1321355349.pdfactinowcete isolation agar ... casein digest cellobiose cetrimide agar base ... lactos lactose lactose lactose lactose broth broth

What is lactose intolerance?. Inability to digest lactose!

What is lactose intolerance?. Inability to digest lactose!

Q1. What is the role of lactose?...Lactase, an enzyme, splits lactose into glucose and galactose. Human milk contains 7.2% of lactose (only 4.7% of lactose in cow’s milk), which

Q1. What is the role of lactose?...Lactase, an enzyme, splits lactose into glucose and galactose. Human milk contains 7.2% of lactose (only 4.7% of lactose in cow’s milk), which