Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie...
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![Page 1: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/1.jpg)
Laboratory Risk ManagementProfessor Michael Gillings
Department of Biological Sciences
Macquarie University, NSW 2109
![Page 2: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/2.jpg)
My personal safety priorities1. People
2. Samples
3. Equipment and infrastructure
Safety is about understanding the risks, not about blindly following safety instructions
![Page 3: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/3.jpg)
Risks to People
• Chemical
• Radiation
• Infectious
• Physical
![Page 4: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/4.jpg)
Chemical SafetyChemical handling and disposal conforms with the most
up to date regulations
![Page 5: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/5.jpg)
Chemical risk management
• Knowledge Laboratory induction, supervision
MSDS documentation
Chemical labeling
• Exposure Protective clothing
Fume hoods
Safety showers / eyewash
• Disposal Segregated waste streams
Area of concern Response
Treat every compound as if it were poisonous
![Page 6: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/6.jpg)
Long Term: Reduce and Replace
Large volume phenol/chloroform DNA extractions
Replace with small volume non-solvent kits
DNA staining with ethidium bromide
Replace with non-carcinogenic stains
![Page 7: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/7.jpg)
Radiation SafetyRadiation hazards are dealt with by induction, radiation
safety officers and specialized containment
![Page 8: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/8.jpg)
Radiation risk managementArea of concern Response• Ultraviolet light Dedicated space / safety visors
• Microwaves Microwave testing
• Radioisotopes Dedicated radiation suite
Licensing / Radiation safety officer
Radiation training / exposure badges
Protective shields and clothing
![Page 9: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/9.jpg)
Long Term: Reduce and Replace
• Significant decline in the use of radioactive materials.
• Replacement with non-isotopic labeling systems and detection methods.
![Page 10: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/10.jpg)
Microbiological SafetyStaff and students are trained in microbiological procedures,
and governed by regulations on biosafety and quarantine
![Page 11: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/11.jpg)
Risks with infectious agentsArea of concern Response
Pathogenic organisms Biosafety approval
/ blood & tissues Good microbiological practice
Protective clothing
Class II cabinets
Zoonotic diseases Biosafety approval
Animal ethics approval
SOPs for animal work
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Physical containmentArea of concern Response
Quarantinable specimens Dedicated PC2 / quarantine lab
Genetically modified organisms Biosafety induction & permits
OGTR inspection & certification
Physical containment laboratories
PC2 operating rules / disposal
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Physical risk managementLaboratories conform to strict building codes (fire ratings, ventilation, emergency stops etc)
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Physical risk managementArea of concern Response
Trips and falls Non-slip flooring, good housekeeping
Needle stick injury Dedicated sharps and glass disposal
Electrical Residual current devices, Tagg testing
Unanticipated risks Rapid response and risk abatement
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Sample managementSamples are extremely valuable, reagents are expensive and
have strict storage requirements
![Page 16: Laboratory Risk Management Professor Michael Gillings Department of Biological Sciences Macquarie University, NSW 2109 michael.gillings@mq.edu.au.](https://reader034.fdocuments.us/reader034/viewer/2022042814/55144e045503462d4e8b4f95/html5/thumbnails/16.jpg)
Why are samples so valuable?• They are difficult and expensive to collect
• They are often irreplaceable
• They are easily destroyed
• They are labor intensive to prepare
Majority of valuable samples are stored in dedicated freezers with back to base alarm systems installed
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Managing equipment and infrastructure
Laboratory space and equipment is expensive. Equipment can also be hazardous
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Managing equipment risksEquipment risk is managed by adherence to specific
Safe Work Procedures (SWPs)
Autoclaves High pressures and temperatures
Centrifuges High rotational velocities
Electrophoresis gear High voltages
-80oC freezer Contact freezer burns
Microwaves Incorrect use
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For your lab inspection:We have stopped research and teaching activity for your tour of the PC2 and teaching laboratories.
Even though you are just visiting, you will still need to conform with safety regulations:
• Closed in footwear
• No food or drink allowed (including chewing gum)
• Don’t touch anything