ORGANOCHLORINE PESTICIDE REPRODUCTIVE EFFECTS IN FATHEAD MINNOWS
LABORATORY REPORT Aquatic Testing Laboratories · PDF file07.01.2014 · FATHEAD...
Transcript of LABORATORY REPORT Aquatic Testing Laboratories · PDF file07.01.2014 · FATHEAD...
LABORATORY REPORT
Date: January 7, 2014
Client: Yolo County District Attorney301 Second StreetWoodland, CA 95695Attn: Heidi D'Agostino
AquaticTesting
Laboratories
Dedicated to providing quality aquatic toxicity testing"
4350 Transport Street, Unit 107Ventura, CA 93003
(805) 650-0546 FAX (805) 650-0756
CA DOES ELAP Cert. No.: 1775
Laboratory No.:Client Sample No.
A-13123001-021/03141A-51A
Sample Control: The samples were received by ATL in new and unopened packaging, with the chainof custody record attached.
Date Received: 12/30/13Date Tested: 01/03/14 to 01/07/14
Sample Analysis: The following analyses were performed on your sample:
CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay (Polisini & Miller 1988).
Attached are the test data generated from the analysis of your sample. All testing wasconducted under the direct supervision of Joseph A. LeMay.
Result Summary:Sample ID. Results
41A- A&H 2X Liq Lavender & Linen FAIL (LC50 < 400 mg/1)42A- A&H Liq Det 2X Clean Burst FAIL (LC50 < 400 mg/1)43A- Gain 2X Original Fresh Liq Deterg FAIL (LC50 < 400 mg/1)44A- Soft Scrub w/Lemon FAIL (LC50 < 400 mg/1)45A- Ivory Liquid Hand Soap Original FAIL (LC50 < 400 mg/1)46A- Palmolive Ultra Pure and Clear FAIL (LC50 < 400 mg/1)47A- Gain Liquid Dish Original Scent FAIL (LC50 < 400 mg/1)48A- SSL Liquid Dishwash Lemon FAIL (LC50 < 400 mg/1)49A- Sun 2X LQ Tropical Breeze FAIL (LC50 < 400 mg/1)50A- Sun Burst Clean & Fresh 2X FAIL (LC50 < 400 mg/1)51 A- Sun Lemon Liquid Dish Soap FAIL (LC50 < 400 mg/1)
Quality Control: Reviewed and approved by:
Joseph A.Laboratory
This report pertains only to the samples investigated and does not necessarily apply to other apparently identical or similar materials. This report is submitted for the exclusive use of theclient to whom it is addressed. Any reproduction of this report or use of the Laboratory's name for advertising or publicity purpose without authorization is prohibited.
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.: Af3
Client/ID:7.X,. Ufa
' TEST SUMMARYSpecies: Pimephales promelas.Fish weight (gm): av: 0.23; min: 0.21; max: 0.25.Reference Toxicant: SDS conducted monthly.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
11 AquaticTestingLaboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
INITIAL
/->-/«/ iiv*
DO PH
24 Hr
DO pH #D
48 Hr
DO PH # D
72 Hr
DO PH #D
96 Hr
DO pH # D
Control A 117 o (17 77 0 ? 0Control B t.o ft* a* 7.7 0 ^77 /ts- O
400 mg/1 A to
400 mg/1 B 'U f. 1° (d
750 mg/1 A t. ft? 9-1 I*
750 mg/1 B
pH Control 71 O £?
Comments: Extraction method: Mechanical shakingNone (aqueous solution)
Dissolved Oxygen (DO) readings in mg/1 O2. Test Aerated: Yes / No pH Control adjusted with HCl/NaOH to pH similar to sample tanks
Initial
Final
CONTROL
Alkalinity
^ ^ mg/1 CaCOj
"J 2- mg/1 CaCO,
Hardness
Y ^ mgA CaC03
y 7 mg/1 CaCO,
HIGH CONCENTRATION
Alkalinity
) 1 mg/lCaCO,
mg/1 CaCO,
Hardness
yjf mg/lCaCO,
— mg/1 CaCOj
Total Number Dead
Control
400 mg/1
750 mg/1
O /20
£o /20
to /20
RESULTS(the checked result applies based on fish survival rates )
^A
/A^
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
^40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.: Ittoot -
Client/ID:
TEST SUMMARYSpecies: Pimephales promelas.Fish weight (gm): av: 0.23; min: 0.21; max: 0.25.Reference Toxicant: SDS conducted monthly.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
11 AquaticTesting
Laboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
pH Control
INITIAL
/"" 5~^V /f¥&
s^o c >
b,0
ttf**l*>((4j1
ft 6f i t
DO
*•>
fctH
f'i
*,l„
*-*
pH
*1f-o
it
**
f.rv
*,/
24 Hr
f'V~/<S /A*"
/^°C
ill
nlm(t9
Wd*f*7
DO
^.>
2 y7-\
?>7>^T
pH
?rlf
7^5'f
«,?
M
g-?
^•y
# D
^O
«7
t<?
^0
Comments: Extraction method: Mechanical shakiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1 O2. Test Aerated: '
Initial
Final
48 Hr
/-T-/V //ysr
^&-°c
'T-7't*"
—
——
A?
DO
?.o1iO
f~
-
-
*••
n1R t^.
PH
777,7
—-^
. -9><1
# D
c?o
^
^
-
w
O
72 Hr
l~t>-<<4 ni0
V^-
°C
•&&
\tf
- —
^~
14. k
DO
£f
^^
-
—£.>
PH
?'?
?7
—
—7f
# D
(J
Cs
——c/
96 Hr
I't-ry t.*<^^
°C
ff'6
/r^
A.
"
/ fy
DO
k*
f<\
~
^
^'?
PH
-,(
7, i^/
—'?«r
# D
Oo^
*-
-V
O*P» ««6»M /- As: .zr/5^ ^^x ^^^
¥es / No pH Control adjusted with HCI/NaOH to pH similar to sample tanks
CONTROL HIGH CONCENTRATION
Alkalinity
3 I mg/1 CaCO3
^X mg/1 CaCO3
Hardness Alkalinity
^ & mg/lCaCOj i/o mg/lCaCOj
7 ^ mg/lCaCOj **v mg/lCaCO,
Hardness
U*( mg/1 CaCO3
— mg/1 CaCO,
Total Number Dead
Control
400 mg/1
750 mg/1
0 /2°2o /20
%C> /20
rt/A\J
RESULTS(the checked result applies based on fish survival rates )
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
^40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.: >7 l~> 1 1 "? tot? A Ol 3
Client/ID: \lo Co^i-y 37
TEST SUMMARY
; max:Species: Pimephales promelas.Fish weight (gm): av: ^?-2>Reference Toxicant: SDS conducted per batch.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
11 AquaticTesting
Laboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number of fish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
INITIAL
i't-W l/V<
7
°C
*,«?
*1
ft .7
(f.7
(\1
ft?
DO
A 7
t.i
*(
*i
14f.i,
PH
•* ff
/u^O
7*?«
M*.*
24 Hr
f-^/V //*5-
^°C
(f '7
r^(^(t/f t?it?
DO
?'7
7-V^^9v»
?/7
So
pH
?-
7^t^D
g-0
&vt<6
# D
oO
to
{*(0
(0
Comments: Extraction method: Mechanical shakiiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1
Initial
Final
CONTROL
Alkalinity
2 f mg/1 CaCO,
3J- mg/1 CaCO,
Hardness
y ^ mg/1 CaCO,
Y 7 mg/1 CaCO,
48 Hr
/-r-/«/ /^^r
^°c
ff-7<1.«-
—s-
—
DO
X o70-
.X
^^
tig f^.tion) ^ — .
PH
?'l
27--
—
—
# D
O
c>
,
-
-
72 Hr
I - & — / " IfJ^fJ/\^-
°C
•&.O
If^
—_^
—
DO
£**7
^—
—
——
PH
2?
?^~-
——
^•>A ^eut v
# D
^
^-
—-
96 Hr
/-7-/<^ ^«^
^°C
^°'f^
^
^
.
S
DO'
^,6
5>r--—-
pH
?-(
1'^
—
-*».
-
# D
C?
£>
—-
—^
-Tte /*w./ 02. Test Aerated: Yes / No
HIGH CONCENTRATION
Alkalinity
) / mg/1 CaCO,
* mg/1 CaCO3
Hardness
Y/ mg/1 CaCO,
•* mg/1 CaCO,
Total Number Dead
Control
400 mg/1
750 mg/1
C> /2°^o /20
<^o /20
x^/^,LX
RESULTS(the checked result applies based on fish survival rates )
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
^40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.:
AquaticTestingLaboratories
Client/ID:
TEST SUMMARY
f ; max:jg.AT_.Species: Pitnephales promelas.Fish weight (gm): av: /?.2>; min:Reference Toxicant: SDS conducted per batch.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
INITIAL
I'J-W l/Vf
?
°c
&,<;
*1«.*
(*4fy*ft'
DO
£7t.L
M9.1
to
t'
PH
7?
fo
*•(
*./
M
*•*
24 Hr
t'</-f</ tfV
^2-
°C
(f7diIt'?r?7illit£
D(f
?')
7-Y?->^e;
«•>
f.?
pH
7-^Ufiu
t*>g^jr1<7
# D
6o(JO
fv
1 0
W
Comments: Extraction method: Mechanical shakiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1
Initial
Final
CONTROL
Alkalinity
*) f mg/1 CaCO,
3 X mSfl CaCO,
Hardness
y ^ mg/1 CaCO,
y 7 mg/1 CaCO,
48 Hr
f-S"vy //vs'
^°c
^•7/t^--^
—
DO
7.0
7.0
~
-
x
^
nfi f^.tion) <^ .
PH
7.777
——-
—
# D
^0--
-
-
72 Hr
/-fc-N ;/?^/ 1— '
°c
l^<?/w—__
—
—
DO
b*I
^L~
—. —
—
PH
"T"*)?-?
——
——
-P^c/^^
#D
£>
^)
——*-
-^
96 Hr
/-?-/y A,*r
^°C
ffO
^—-
^
~
DO
£«>
f 'f
—
/-.
X
pH
7,^>-/^^
^^__^
# D
<9
^
-X"
—
-
'•«. A;«,,A^02. Test Aerated: Yes / No
HIGH CONCENTRATION
Alkalinity
~}\1 CaCO,
* mg/1 CaCO,
Hardness
y / mg/1 CaCO,
* mg/1 CaCO,
Total Number Dead
Control
400 mg/1
750 mg/1
0 /20
2o /20
^ /20
>f
yv/
RESULTS(the checked result applies based on fish survival rates )
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
^40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.: >?/^/3t ~>t9t?t~O
Client/ID: Yo/gIX1~-+\T SUMMARY
Species: Pimephales promelas.Fish weight (gm): av: 0.3.}', min: Q. t / ; max: jReference Toxicant: SDS conducted per batch.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
T ( AquaticTestingLaboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
INITIAL
t'J'W l/Vf
?
°C
fc*
*1
(?•'
(itH 7
n.i
DO
*7It
t>t
*•!*.\. i
pH
7?fro
fc?
*Y
J-Y».)
24 Hr
/-*-/v //yr
^°C
0-7it'(v?
ft*<l*(T,*
DO
?J
7,ifft>eo9.1<iO
PH
7.y7$'•r*.**,«^^
#D
O
o(v(0
(0
U
Comments: Extraction method: Mechanical shakiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1
Initial
Final
48 Hr
/-*-•/*' //ys-
^°C
/r7,?j
^^^
DO
?•<*
7-0_
^
-w.
^
"8 ^"-tion) < *1" .
pH
?77.7--
——
#D
^?O^--
—
72 Hr
J-fr-Yf f/?^
/^v
°C
?f
-~
. —
—— .
DO
fc^*^r
fr>^
——
—
pH
??
7-7
——
——
-/•** ,/f.X .
#D
&
~
—-^-
96 Hr
/ - /vy ^>?r
^°c
fTid
rf ^---
• >s
DO
/^
-Cf
"S
<
r
•
pH
^Y
^ D
^^
--
#D
<9
c>
.
-
-
^
aV*,u A.y^.
02. Test Aerated: Yes / No
CONTROL HIGH CONCENTRATION
Alkalinity
7 f mg/1 CaCO,
3 JL mg/1 CaCO,
Hardness Alkalinity
V mg" CaCO, ^ ^ mg/1 CaCO,
y 7 mg/1 CaCO, — mg/1 CaCO,
Hardness
<ja mg/1 CaCO,
— mg/1 CaCO,
Total Number Dead
Control
400 mg/1
750 mg/1
O /20
" 0 /20
^o /20
RESULTS(the checked result applies based on fish survival rates )
,4
A^XI/
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
^40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.: I">11
Client/ID: Ya/g I$oootf6/t>6
TEST SUMMARY
; max:Species: Pimephales promelas.Fish weight (gm): av: ^ .Jt>; min: Q.Reference Toxicant: SDS conducted per batch.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
T AquaticTestingLaboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
INITIAL
I-}- ti<«
DO PH
24 Hr
rxf pH # D
48 Hr
DO PH # D
72 Hr
DO pH
96 Hr
DO pH #D
Control A I* (f-7 c?Control B 7-7 7-7 O
400 mg/1 A
400 mg/1 B (14 tv
750 mg/1 A it)
750 mg/1 B \U *7 L *•(
Comments: Extraction method: Mechanical shakingNone (aqueous solution) s*~.
Dissolved Oxygen (DO) readings in mg/102. Test Aerated: Yes / No
Initial
Final
CONTROL
Alkalinity
~) f mg/lCaCO,
3J^ mg^CaCO,
Hardness
y £ mg/1 CaCOj
V > mg/1 CaCOj
HIGH CONCENTRATION
Alkalinity
£0 mg/lCaCO,
— mg/1 CaCO,
Hardness
t/ ^ mg/1 CaCOj
~~ mg/1 CaCO3
Total Number Dead
Control
400 mg/1
750 mg/1
0 /2°^o /20
^> /20
RESULTS(the checked result applies based on fish survival rates )
A
rf.<X
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
^40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.: >?
Client/ID:
TEST SUMMARYSpecies: Pimephales promelas.Fish weight (gm): av: a-H"); min: Q.3L ( ; max:Reference Toxicant: SDS conducted per batch.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
T [ AquaticTestingLaboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
INITIAL
l-l'tlf l/Vf
?
°C
*,0
*1
ft*
Mq.1r f <
DO
A 7I.Lt,r
s*<uf, *
PH
?ef
$i0
M*f«,/<.l
24 Hr
/-y-/v tits-
°C
it<7ill1*7hi(14fp
<^DO
7»*Mjtu
21
7^
PH
7,7«f,0^^«0<^u
# D
o0
If?(0
\P
Comments: Extraction method: Mechanical shakiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1
48 Hr
1-S--/Y KY*
°c
f?7/f-—-~-
DO
?.<?
7.0
^
^
-
-
n« t^.
pH
7-777-
———
#D
c?0
V
--
^>
72 Hr
l-*b~H \\lf>rj^\c
?00lj* /
—
——
DO
''I
lf*f^^
~
—
—
—
pH
7.7
7.7~-
— -
—
L. — •
#D
O0-
——-
96 Hr
l*7-lf r~VT
--°c
n$(ti—^--
DfT
/^^T-
—--
pH
7.^
?-/
,_
. —
—^
# D
^?
O
—-
-
-
-/="'>* WVt/ -'A. M/M./' ^v/-tion) /"^.
02. Test Aerated: Yes / No
Initial
Final
CONTROL
Alkalinity
^ f mg/1 CaCO,
3JL mg/1 CaCO,
Hardness
y^ mg/ICaCOj
*/ 7 mg/1 CaCOj
HIGH CONCENTRATION
Alkalinity
^ / mg/1 CaCOj
*~ mg/1 CaCO,
Hardness
</ ^ ""g/1 CaCO,
"" mg/1 CaCO3
Total Number Dead
Control
400 mg/1
750 mg/1
0 /20
" 0 /20
2,0 /20
RESULTS(the checked result applies based on fish survival rates )
-4^v/
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
^40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.:
Client/ID: Va/g
TEST SUMMARY
; max:Species: Pimephales promelas.Fish weight (gm): av: <7-2>; min:Reference Toxicant: SDS conducted per batch.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
V ( AquaticTestingLaboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
INITIAL
I'J'W t/Vf
7°c
*,*
*1<?,{(ft(\(<r*
DO
A 7t.l
6tl
f.f
**
*. ?
PH
?f
uf-l,,(M$•(
24 Hr
/-v-/v tlvr
^°c
ft -7rt/(t7<U
(^m
DO
?r^7.Y
?-»777y75-
pH
tf
11
f~0
90
9*
f.O
# D
oo(.0
(0
(t;\
Comments: Extraction method: Mechanical shakiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1
Initial
Final
CONTROL
Alkalinity
~) f mg/1 CaCO,
5 JL mg/1 CaCOj
Hardness
V 6 ms" CaC°3
y > mg/l CaCO,
48 Hr
f-f'tf IKS'
<^°C
T-7( ty^'-^
DO
7i0?.o-,
^_-
ng (^.tion) / *" .
PH
7-7If-
—
—"
# D
d>
0
-•w
l.
-
72 Hr
/-£ -/V H3^
^°c
-zip(tf
——
—
DO
^f
^
—^
— -
pH
7.7
7-7
——
—-FH J*A .
#D
^
^
—
—-
96 Hr
/-7vy /^yr
^°C
^f.°
t*
-
-*
^
DO
^6>ct
—^
^
pH
^/?,^
—•
—^
# D
O^>^^
^ ,
—,-AU ,v*-^<*02. Test Aerated: Yes / No
HIGH CONCENTRATION
Alkalinity
_} mg/1 CaCOj
"~ mg/1 CaCOj
Hardness
(j «- mg/1 CaCO,
— mg/1 CaCO,
Total Number Dead
Control
400 mg/1
750 mg/1
6 /20
^0 /20
2^> /20
RESULTS(the checked result applies based on fish survival rates )
S<LX
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
£40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.: >? /> /3 t
Client/ID: % (a t z if 3 7o »f I
TEST SUMMARY
; max:Species: Pimephales promelas.Fish weight (gm): av: a-H); min: tf>.Reference Toxicant: SDS conducted per batch.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
*T ( AquaticTestingLaboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
INITIAL
('}•!*/ l/Vf
7
°C
T*,0
*1
(U
(H
rt,£lf.7
DO
£7
1.1,
1.1f>6
%>*l.l,
PH
7?U
h*itr,
?.2
24 Hr
f-f'SY ffYS
^"C
07ftflt*lt-7^7/l(
DO
7->W7+
to»<(&t>
pH
7-f7.^.
$7
>r*-5
# D
ooI*
(«>
(^f o
Comments: Extraction method: Mechanical shakiiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1 <
Initial
Final
48 Hr
^rv*/ //ys^
^°c
ff '7I t - J-
-
-^
DO*
7'°X«
». .--s
IS t^.tion) /-"' .
PH
?.;
7-7._
——
# D
(?
0
^1
--
-
72 Hr
/~£-/H r/5^^-v
°C
Z^
(^—— .
——
DO
^-f
^>
—' —
—
pH
?^
7^—
———
-F--51 A«t.
#D
£>»
^
——
—-
96 Hr
f -? - /y /^y5-
^°C
Orfl
f 4 ^
•H
*~~
-
^
DO
^e>
$;?---
^
pH
?-^
7 ^_ _,--
# D
^>
^
•N
-
-
-
-•/W- l«!t,\, tr
02. Test Aerated: Yes / No
CONTROL HIGH CONCENTRATION
Alkalinity
~> \1 CaCO,
^ **- mg/1 CaCO,
Hardness Alkalinity
y^ mg/1 CaCO, Yj mg/1 CaCO,
«/ 7 mg/1 CaCO, — mg/1 CaCO,
Hardness
y / mg/l CaCO,
— mg/1 CaCO,
Total Number Dead
Control
400 mg/1
750 mg/1
C /20
^0 /20
• o /20
RESULTS(the checked result applies based on fish survival rates )
rf
A ,IX
PASSED
^ FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/l cone.)
^40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIO ASSAY
Lab No.: A 3o»/~O
Client/ID:
TEST SUMMARYSpecies: Pimephales promelas.Fish weight (gm): av: 0.23; min: 0.21; max: 0.25.Reference Toxicant: SDS conducted monthly.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
AT AquaticTesting
Laboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number offish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
pH Control
INITIAL
'-3-fV INS
^°C
*.o
ItfI1.\
f?.'Ill(T.{
DO
*•?
9,6
*,7
*• >
9 7*•**-y
pH
£79.o
7$
0
<$r,?r./
24 Hr
l~*fv //vs-
^°C
'T-7
'Vf f j tttimft?
DO
7r*
?.y9'7
^c */1 ^r
^/zt
PH
7«7.^Z]
f-o
^7?.?
*>Y
# D
C>^<7
(17
^19
O
Comments: Extraction method: Mechanical shakiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1 O2. Test Aerated: '
Initial
Final
48 Hr
f- r-/y //y»~
^°c
(77f t ?-"-^
f t ?
DO
7-*r«_-"-^/
ng iX.tion) -^ .V'es / No ]
pH
7'77-7
—-
^
^
#Y
# D
f?
c?x
s
»-
^
6
72 Hr
/-£~Af U";tf^\C
7^
(1-1
— .
—— .
—(^
DO
£-f
*^^^
^
^
—
pH
^?
7-7^
—— .—
7^
# D
£>
£
-
—
—
—&
96 Hr
^7-/y ^y^T
^°C
f^-C
^
-
-
-
/ ty
DO
&0
y-T—---
5-
pH
7.*?(>
—^^-
2C
# D
e?o-
—
—^^
-h ;+.•*! />H 7- ^sH Control adjusted with HCl/NaOH to pH similar to sample tanks
CONTROL
Alkalinity
/ ( mg/1 CaCO,
3 J~ mg/1 CaCO,
Hardness
«/^ mg/1 CaCO,
V7 m^CaCO,
HIGH CONCENTRATION
Alkalinity
U 1 mg/1 CaCO,
— - mg/1 CaCO,
Hardness
Uf mg/1 CaCO,
— mg/1 CaCO,
Total Number Dead
Control
400 mg/1
750 mg/1
O /20
20 /20
^o /20
RESULTS(the checked result applies based on fish survival rates )
/4
/•*,\sPASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
£40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
FATHEAD MINNOW HAZARDOUS WASTESCREEN BIOASSAY
Lab No.:
Client/ID: Vg/g Ca*«+»
TEST SUMMARY
/ ; max:Species: Pimephales promelas,Fish weight (gm): av: <y.3>; min:Reference Toxicant: SDS conducted per batch.Test chamber volume: 10 liters.Temperature: 20 +/- 2°C.Aeration: none, unless D.O. drops below 5.0 mg/1.Number of replicates: 2.Dilution water: Soft reconstituted water (40-48 mg/1 CaCO3).
*T [ AquaticTestingLaboratories
Source: Thomas Fish.Regulations: CCR Title 22.Test Protocol: California F&G/DHS 1988.Endpoints: Survival at 96 hrs.Test type: Static.Feeding: None.Number of fish per chamber: 10.Photoperiod: 16/8 hrs light/dark.
TEST DATA
Date/Time:
Analyst:
Control A
Control B
400 mg/1 A
400 mg/1 B
750 mg/1 A
750 mg/1 B
INITIAL
('}•«/ l/Vf
7°c
l>,0
*1Mf t *min
DO
A 7£1
fcjr
*.'t,7
&°l
PH
?t
fro
9>>
t.7
*;*?
24 Hr
/-Y-/V ''f5-
<&°c
«T7ffY/K(17
'17tit
DO
?r>7-V^?9'?^.T
^,T
pH
?•*
7*
s.?«•?fc.yV^
# D
0
^1°\. 0
1.0
Comments: Extraction method: Mechanical shakiiNone (aqueous solu
Dissolved Oxygen (DO) readings in mg/1
Initial
Final
CONTROL
Alkalinity
"} f mg/lCaCO,
3^L mg/1 CaCO,
Hardness
y£ mg^CaCO,
*/ 7 "'S'1 CaC°3
48 Hr
I ' f ' f t f /rrs~
^°c
11?nt
—*
*>-
DO
?.07o
^
-
-
-
n* ^.tion) ^" .
pH
>.77.7-
*»
v
s
#D
i?
0
^
-^
»»
•c
72 Hr
/.6>-rv ^3^'> — .
°c
2?^
(^
-
———
DO
^
^—
—— -
—
PH
2?
?7
—
—
—~
- f-'<> A c/^U
#D
^^<2?
——
—
—
96 Hr
I-?-/Y toys'
^"C
(1?n*-^
—
—
DO
t>°
W
-
^
s
pH
?./
7/N
-
-
—
# D
^>
^>
^
-
-•*•
u/.' /*»•'••« 'it/Mi t Aou/'
02. Test Aerated: Yes / No
HIGH CONCENTRATION
Alkalinity
) / mg/1 CaCO,
mg/1 CaCOj
Hardness
Y/ mg/lCaCO,
~~ mg/1 CaCO3
Total Number Dead
Control
400 mg/1
750 mg/1
0 /20
2^ /20
Q*> /20
M/^iX
RESULTS(the checked result applies based on fish survival rates )
PASSED
FAILED
FAILED
LC50 > 750 mg/1 (<40% dead in 750 mg/1 cone.)
£40% dead in 750 mg/1 (close to passing - definitive test recommended)
LC50 < 400 mg/1 (>60% dead in 400 mg/1 cone.)
/^A%
Testing Facility
Facility name: Aquatic Testing Laboratories
Facility address: 4350 Transport Street, Unit 107, Ventura, CA93003
LlSample Number
41A
42A
43A
44A
45A
4SA
47A
48A
49A
50A
S1A
52A
53A
S4A
55A
56A
S7A
58A
59A
60A
Description
A&H 2X LIQ LAVENDER & LINEN
A&H LIQ DET 2X CLEAN BURST
GAIN 2X ORIGINAL FRESH LIQ DETERG
SOFT SCRUB W/ LEMON
IVORY LIQUID HAND SOAP ORIGINAL
PALMOLIVE ULTRA PURE AND CLEAR
GAIN LIQUID DISH ORIGINAL SCENT
SSL LIQUID DISHWASH LEMON
SUN 2X LQ TROPICAL BREEZE
SUN BURST CLEAN & FRESH 2X
SUN LEMON LIQUID DISH SOAP
SUN SATIONS AUTO DISH GEL
TIDE LQ PODS OCEAN MIST
LYSOL TOILET BOWL CLEANER
PALMOLIVE ORIGINAL
CLOROX TOILET BOWL CLEANER BLCH G
CLOROX TOILET BOWL GREEN WORKS
DRANO PROFESSIONAL STRENGTH
SNUGGLE BLUE SPARKEL
ALL OXI-ACTIVE STAINLIFTERS WATER
OFFICE OF THE DISTRICT ATTORNEYSAN JOAQUIN COUNTY
222 E Weber Avenue, Suite 678, Stockton, CA 95202P.O. Box 990, Stockton, California 95201
Telephone: (209) 468-2400 Fax: (209) 468-03 1 4
CHAIN OF CUSTODY RECORD
Manufacturer UPC
3320009332
3320009470
3700012784
2340000865
3700040312
3500046106
3700086176
71754411703
7261370896
7261370809
7261316008
7261324025
3700050962
1920002522
3500046112
4460030619
4460000451
1980040109
7261345171
7261345497
Quantity
75 oz
150 oi
5002
24 oz
48 oz
25 oz
24 oz
40 oz188 oz,
125 loads
45.4 oz
20 oz
75 oz27 oz,31 ct
24 oz
25 oz
24 oz
2402
80 oz64 oz,
26 loads
150 oz
Main Ingredient / Or Usage / NOTES
,
S
o
Please send results to:
Name: nvestigator Heidi D'Agostino
Email: [email protected]
Fax: 530-666-8423
Time sampled
Sample containerdescription
Method of sampling:Composite (C) Grab (G)
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
Size of sample container(include units)
200g (min)
200( (min)
200g (min)
200s (min)
200< [mini
200« (mini
200( (min)
200s (min)
200s (mini
2008 (min)
200g (min)
200B (min)
200g (min)
200« (min)
2008 (min)
2008 (min)
2008 (mini
2008 (min)
2008 Imin)
2008 Imin)
ample containerPlastic (P) Metal
r) VOA(V)
•sssill£ a S
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
Evidence tape: Yes(Y)No(N)
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
Transported In Iced cooler:Yes(Y) No(N)
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
Requiredanalysis
96-hour fishbloassay
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
NOTE: All Samples were taken at 222 E. Weber Ave.. Room 678. Stockton. CA 95202
NarjA / Title
Relinquished by:
Received by: