Laboratory Notebook and Documentation

GLP

Identifying your notebook

Notebook reference numbers

Footnotes

Laboratory Notebook and Documentation

• Reflection of our work; • quality of work = quality of results

• Quality over quantity

• Ability to be repeated

• Mandatory for GLP

Why is notebook documentation important?

Good Laboratory Practice

USDA, EPA, FDA define practices and documents. These agencies regulate labs.

“Your data may have to be explained, defended, reconstructed or repeated without your assistance, so others must be able to understand what you did” *

*http://users.stlcc.edu/departments/fvbio/Lab_Practices_GLP_STLCC.htm

What is GLP?

Identify your notebook. Who does this notebook belong to?

GLP identification scheme is different for all establishments. Some require it be identified as a project code or after the scientists initials. A general identification scheme will be First and Last initial with consecutive numbers. Ex) Marleen Cook’s first notebook = MC01 Marleen Cook’s second notebook = MC02

In the event a new member arrives with the same initials, the notebook ID will be supplemented with a middle initial in the identification. Ex) Mike Romeo Charlie’s first notebook = MRC01

What will your notebook identification be?

First steps to a successful notebook

Identify your notebook and make it clear and noticeable on the front cover and spine.

Fill out all the information in the inside cover. Anything that does not apply should have “N/A” and anything that will apply at a later date should remain blank.

Table of contents should be filled out as new pages are filled in with the title assigned to the page.

Ex) Cell passage & Media prep Page 52

Where do I start?

Numerical reference to any activity done in the lab.

Nomenclature: Notebook ID-Page-LineEx) Marleen Cook’s first lab book. Entry on Page 2 on line 4 = MC01-02-04

Must be noted in a timely manner, clear without disruption to original entry i.e. whiteout, erase, write over, discard, or scratched out.

All measuring units should be clearly stated, times and dates should also be clearly stated with a differentiation of AM and PM or military time should be used.

If any information is change/altered or added you must make a footnote.

When attaching a document an attachment line must be present with initials and date.

Notebook references

Footnotes are notation to what you have changed/altered added in a notebook entry with a notation of why, who and when the action was performed.

Every footnote should be different from the last unless the same action occurred

Ex) entry error that has occurred multiple times on the same day bythe same person.

MC01-04-02 Media Prep for ABC123 cells

50ml 450 ml of RPMI along with 45ml * of FBS were added to an autoclaved 500ml botteel.* Stored in -20ºC.^

bottle 4ºCPrep by: MDC 19July2010

* ee MDC 19July2010^ Change in temperature

suggested by PI Dr. Smith. M19July2010

MC01-04-02 Media Prep for ABC123 cells

50ml 450 ml of RPMI along with 45ml * of FBS were added to an autoclaved 500ml botteel.* Stored in -20ºC.^

bottle 4ºCPrep by: MDC 19July2010

* ee MDC 19July2010^ Change in temperature

suggested by PI Dr. Smith. M19July2010

Footnotes

Reagent Prep IMDM growth media

450 of IMDM plus 50ml of FBS was added to an autoclaved 500ml boj bottle. Stored at 4ºC.

What did this person do wrong? HINT: There are 4 mistakes. What are they?

Test yourself!

????-??-??????-??-?? Reagent Prep IMDM growth media

450 of IMDM plus 50ml of FBS was added to an autoclaved 500ml boj bottle. Stored at 4ºC.

1. Notebook reference?2. Units?3. Reason for correction?4. Who made the entry?

Answers