Laboratory Equipment

The figure was adopted from http://vip.busytrade.com/huaou (Dec 2006)

volumetric cylinders

watch glasses

beakers

The figures were adopted from z http://vip.busytrade.com/huaou (Dec 2006)

volumetric flasks

The figures were adopted from http://vip.busytrade.com/huaou and http://www.silcom.com/~adbrown/tutor/images/Vol-flask.gif (Dec 2006)

measuring of a precise volume

„meniscus“= a concave

upper surface that forms on a

liquid in a tube as a result of

surface tension

filling the volumetric flask

glass pipettes

filling the pipette using the pipette pump

pipette pump

insertion of the pipette into the pipette pump: carefully, but tightly

meassuring of a precise volume of 5

mL

4,49 mL 4,85

mL

5,0 mL

The figures are from http://www.uwplatt.edu/chemep/chem/chemscape/labdocs/catofp/measurea/volume/pipet/page.htm (Dec 2006)

measuring out a precise volume by the doser

dosertest tubes holder

doser

storage bottle

dosing plunger

wheel for volume

adjustment

titrimetric flask

burette

„titration“

burette

burette filler

graduated tube

storage bottle

dosing stopcock

filling the burette

using the burette

automatic pipette

plastic tip pipetting button

emptying filling

The figure was adopted from the CD-ROM: Encyklopedie laboratorní medicíny pro klinickou praxi 5. Kolektiv autorů, ed. A. Jabor,M. Zámečník, SEKK s r.o., Pardubice, Katedra klinické biochemie IPVZ Praha, 2005, hypertext.

ISBN 80-238-9775-6

the pipette can be used solely with a plastic tip

automatic pipette for the volume range: 10 – 100 l

yellow tip is destined for „yellow pipette“

automatic pipette for the volume range: 10 – 100 l

yellow tip is destined for „yellow pipette“

automatic pipette for the volume range: 100 – 1000 l

blue tip is destined for „blue pipette“

automatic pipette for the volume range: 100 – 1000 l

blue tip is destined for „blue pipette“

volume adjustment of the automatic pipette

Never adjust volume out ofthe allowed range!

turn slowly

stendby 1st position 2nd position

holding the pipette

filling emptying

Pipetting step by step

1st stendby 1st 2nd

The figure is from: Encyklopedie laboratorní medicíny pro klinickou praxi 5. Kolektiv autorů, ed. A. Jabor, M. Zámečník, SEKK s r.o., Pardubice, Katedra klinické biochemie IPVZ Praha, 2005, hypertext. ISBN 80-238-9775-6

Removing of solution drops from the tip surface

the drops can be removed only in a direction from up to down

stendby position 1st position

(= filling the pipette)

emptying the pipette (2nd position)

tip ejector button

spectrophotometer

pH meter

with an electrode immersed into buffer solution

chromatographic chamber

with a thin layer inside (TLC chromatography)

fume cupboar

d

chromatographic chambers

water bath (thermostat)

equipment for gel electrophoresis

power suply (direct current)

electrophoresis chamber

applicator

containers for staining and destaining gel