Lab Meeting August 8, 2013
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Transcript of Lab Meeting August 8, 2013
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Lab Meeting August 8, 2013
By: Charis Fernandez
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Techniques Learned
• Gel Electrophoresis• Cell culture• Nanoparticle preparation• Lyophilization• Cytotoxicity
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1: Empty rHDL2: Myristoyl valrubicin3: Apo A1 valrubicin4: Thyroglobulin5: Plasma serum6: Bovine serum albumin7: Human HDL
Native PAGE Bis-Tris Gel with Tricine Buffer
Thyroglobulin MW: 660,000 DaBSA MW: 66,500 DaHuman HDL MW: 70,000-360,000 DaEstimated rHDL MW: ~170,000 Da
24 hour run
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1: Empty rHDL2: Myristoyl valrubicin3: Apo A1 valrubicin4: Thyroglobulin5: Plasma Serum6: Bovine Serum Albumin7: Human HDL
Native PAGE Bis-Tris Gel with Tricine Buffer8 hour run
Thyroglobulin MW: 660,000 DaBSA MW: 66,500 DaHuman HDL MW: 70,000-360,000 DaEstimated rHDL MW: ~170,000 Da
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Myr-5A-Cisx Nanoparticle Size
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Myr-cisxEmpty rHDL
Concentration (μM)
% C
ell S
urvi
val
MTT Cytotoxicity
Myr-5A-Cisx and Empty rHDL on SJ-N-KP cell line
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Size and NMR to determine percent drug incorporated
Empty Nanoparticle Samples
• 0.5 mg Myr-5A peptide• 1.0 mg Myr-5A peptide• 2.0 mg Myr-5A peptide
Encapsulated 50 µl Cisplatin Derivative Samples
• 0.5 mg Myr-5A peptide• 1.0 mg Myr-5A peptide• 2.0 mg Myr-5A peptide
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Future Studies
• Western blot of Neuroblastoma cell lines• Cytotoxicity of Cisplatin derivative on different
Neuroblastoma cell lines• Effect of encapsulated Cisplatin derivative on normal
healthy cells