Lab-2 biore
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Transcript of Lab-2 biore
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Datostiempo [min] Biomasa (abs) Glucosa (abs) Glucosa (g/L) Biomasa (g/L) LN (X)
0 0.340 1.551 6.517 0.9770 -0.02315 0.280 1.689 7.097 0.8029 -0.22030 0.214 1.743 7.324 0.6113 -0.49245 0.240 1.654 6.950 0.6868 -0.37660 0.336 1.533 6.441 0.9654 -0.03575 0.230 1.730 7.269 0.6577 -0.419
110 0.2 1.721 7.231 0.5707 -0.561125 0.216 1.679 7.055 0.6171 -0.483140 0.244 1.433 6.021 0.6984 -0.359155 0.22 1.525 6.408 0.6287 -0.464170 0.239 1.567 6.584 0.6839 -0.380185 0.263 1.7 7.143 0.7535 -0.283200 0.268 1.561 6.559 0.7680 -0.264215 0.292 1.627 6.836 0.8377 -0.177230 0.314 1.634 6.866 0.9016 -0.104245 0.316 1.69 7.101 0.9074 -0.097260 0.325 1.366 5.739 0.9335 -0.069
Yx/s 0.1193mu 0.0038
0 50 100 150 200 250 300
-0.600
-0.500
-0.400
-0.300
-0.200
-0.100
0.000
f(x) = 0.0037844426x - 0.995849406R = 0.9489239523
Evol. BiomasaCrecimiento exponencialLinear (Crecimiento exponencial)
Tiempo (min)
Ln(X)
0 50 100 150 200 250 3005.8006.0006.2006.4006.6006.8007.0007.2007.400
Evol. Glucosa
Evol. Glucosa
tiempo (min)
Conc Glucosa (g/L)
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Estndar Glucosa (abs) i P
0.238
1.366 1 0.02941.433 2 0.08821.525 3 0.14711.533 4 0.20591.551 5 0.26471.561 6 0.32351.567 7 0.38241.627 8 0.44121.634 9 0.50001.654 10 0.55881.679 11 0.61761.689 12 0.67651.69 13 0.73531.7 14 0.7941
1.721 15 0.85291.730 16 0.91181.743 17 0.9706
0 0.002 0.004 0.0060
0.20.40.60.8
11.21.41.6
f(x) = 344.5080536519x + 0.0033944302R = 0.9995185898
Linear ()
Peso Seco [g/mL]
Absorbancia a 600 nm
1.3 1.35 1.4 1.45 1.5 1.55 1.6 1.65 1.7 1.75 1.80.00
0.20
0.40
0.60
0.80
1.00
1.20
GPN glucosa
0 50 100 150 200 250 300
-0.600
-0.500
-0.400
-0.300
-0.200
-0.100
0.000
f(x) = 0.0037844426x - 0.995849406R = 0.9489239523
Evol. BiomasaCrecimiento exponencialLinear (Crecimiento exponencial)
Tiempo (min)
Ln(X)
0 50 100 150 200 250 3005.8006.0006.2006.4006.6006.8007.0007.2007.400
Evol. Glucosa
Evol. Glucosa
tiempo (min)
Conc Glucosa (g/L)
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0 0.002 0.004 0.0060
0.20.40.60.8
11.21.41.6
f(x) = 344.5080536519x + 0.0033944302R = 0.9995185898
Linear ()
Peso Seco [g/mL]
Absorbancia a 600 nm
1.3 1.35 1.4 1.45 1.5 1.55 1.6 1.65 1.7 1.75 1.80.00
0.20
0.40
0.60
0.80
1.00
1.20
GPN glucosa
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t[min] Biomasa Glucosa Estndar Glucosa (g/L)0.0 0.6020 1.7100
0.2680
6.380620.0 0.5230 2.0520 7.656740.0 0.5130 1.8710 6.981360.0 0.5210 1.9330 7.212780.0 0.5270 1.3600 5.0746
100.0 0.5760 1.7830 6.6530120.0 0.5950 1.8080 6.7463140.0 0.6110 1.7670 6.5933170.0 0.7160 1.7810 6.6455190.0 0.7510 1.7330 6.4664210.0 0.7870 1.7430 6.5037230.0 0.8310 1.7140 6.3955250.0 0.9100 1.7910 6.6828270.0 1.0360 1.7300 6.4552290.0 1.0540 1.6980 6.3358310.0 1.2360 1.6630 6.2052330.0 1.2720 - #VALUE!
Yx/s 10.494mu 0.0036
0.0 100.0 200.0 300.0 400.00.00000.20000.40000.60000.80001.00001.20001.4000
f(x) = 0.003574562x + 0.1019027615R = 0.9785743813
Chart Title
Evolucin de biomasaCrecimientoLinear (Crecimiento)
Axis Title
Axis Title
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Biomasa (g/L) Ln (X) Glucosa i1.7375 0.5525 1.3600 1.00001.5082 0.4109 1.6630 2.00001.4792 0.3915 1.6980 3.00001.5024 0.4071 1.7100 4.00001.5198 0.4186 1.7140 5.00001.6621 0.5081 1.7300 6.00001.7172 0.5407 1.7330 7.00001.7637 0.5674 1.7430 8.00002.0684 0.7268 1.7670 9.00002.1700 0.7747 1.7810 10.00002.2745 0.8218 1.7830 11.00002.4023 0.8764 1.7910 12.00002.6316 0.9676 1.8080 13.00002.9973 1.0977 1.8710 14.00003.0495 1.1150 1.9330 15.00003.5778 1.2748 2.0520 16.00003.6823 1.3035
0.0 50.0 100.0 150.0 200.0 250.0 300.0 350.05.906.006.106.206.306.406.506.606.706.80
Consumo Glucosa
0.0 100.0 200.0 300.0 400.00.00000.20000.40000.60000.80001.00001.20001.4000
f(x) = 0.003574562x + 0.1019027615R = 0.9785743813
Chart Title
Evolucin de biomasaCrecimientoLinear (Crecimiento)
Axis Title
Axis Title
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P0.03130.09380.15630.21880.28130.34380.40630.46880.53130.59380.65630.71880.78130.84380.90630.9688
1.3000 1.4000 1.5000 1.6000 1.7000 1.8000 1.9000 2.0000 2.10000.0000
0.2000
0.4000
0.6000
0.8000
1.0000
1.2000
Chart Title
0.0 50.0 100.0 150.0 200.0 250.0 300.0 350.05.906.006.106.206.306.406.506.606.706.80
Consumo Glucosa
-
1.3000 1.4000 1.5000 1.6000 1.7000 1.8000 1.9000 2.0000 2.10000.0000
0.2000
0.4000
0.6000
0.8000
1.0000
1.2000
Chart Title
-
t abs biomasa (600 nm) abs glucosat (505 nm) estndar Glucosa (g/L)0.00 0.5550 0.7250
0.2720
2.665420.00 0.5570 0.7430 2.731640.00 0.5570 0.8080 2.970660.00 0.6500 0.7660 2.816280.00 0.5970 0.7040 2.5882
100.00 0.6880 0.6760 2.4853120.00 0.7190 0.6660 2.4485140.00 0.7690 0.5690 2.0919160.00 0.9250 1.2340
0.2900
4.5368180.00 0.9830 1.6610 6.1066200.00 1.0390 1.7920 6.5882220.00 1.0980 1.5920 5.8529240.00 1.1830 1.2980 4.7721260.00 1.2280 1.1420 4.1985280.00 1.2550 0.9850 3.6213400.00 1.6540 0.2640 0.9706420.00 1.6220 0.2240 0.8235440.00 1.5940 0.1390 0.5110460.00 1.6770 0.0660 0.2426480.00 1.5840 0.0000 0.0000500.00 1.8000 0.0000 0.0000520.00 1.7860 0.0000 0.0000540.00 1.8080 0.0000 0.0000560.00 1.7720 0.0000 0.0000
Yx/s 1.1206mu 0.0032
0.0 100.0 200.0 300.0 400.0 500.0 600.00.0000.2000.4000.6000.8001.0001.2001.4001.6001.800
f(x) = 0.0032259318x + 0.3881914192R = 0.944964649
Chart Title
Evol BiomasaLinealLinear (Lineal)
Axis Title
Axis Title
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Biomasa (g/L) LN(X) abs glucosat (505 nm)i1.6011 0.4707 0.0000 1.00001.6069 0.4743 0.0000 2.00001.6069 0.4743 0.0000 3.00001.8769 0.6296 0.0000 4.00001.7230 0.5441 0.0000 5.00001.9872 0.6867 0.0660 6.00002.0772 0.7310 0.1390 7.00002.2223 0.7985 0.2240 8.00002.6751 0.9840 0.2640 9.00002.8435 1.0450 0.5690 10.00003.0060 1.1006 0.6660 11.00003.1773 1.1560 0.6760 12.00003.4240 1.2308 0.7040 13.00003.5546 1.2682 0.7250 14.00003.6330 1.2901 0.7430 15.00004.7912 1.5668 0.7660 16.00004.6983 1.5472 0.8080 17.00004.6170 1.5297 0.9850 18.00004.8579 1.5806 1.1420 19.00004.5880 1.5234 1.2340 20.00005.2149 1.6515 1.2980 21.00005.1743 1.6437 1.5920 22.00005.2382 1.6560 1.6610 23.00005.1337 1.6358 1.7920 24.0000
0.0 100.0 200.0 300.0 400.0 500.0 600.00.0000.2000.4000.6000.8001.0001.2001.4001.6001.800
f(x) = 0.0032259318x + 0.3881914192R = 0.944964649
Chart Title
Evol BiomasaLinealLinear (Lineal)
Axis Title
Axis Title
-
P0.02080.06250.10420.14580.18750.22920.27080.31250.35420.39580.43750.47920.52080.56250.60420.64580.68750.72920.77080.81250.85420.89580.93750.9792
0.0000 0.2000 0.4000 0.6000 0.8000 1.0000 1.2000 1.4000 1.6000 1.8000 2.00000.0000
0.2000
0.4000
0.6000
0.8000
1.0000
1.2000
GPN
-
0.0000 0.2000 0.4000 0.6000 0.8000 1.0000 1.2000 1.4000 1.6000 1.8000 2.00000.0000
0.2000
0.4000
0.6000
0.8000
1.0000
1.2000
GPN
-
t [min] Biomasa Glucosa Estndar Estndar Glucosa (g/L) Biomasa (g/L) LN(X)0.00 0.675 0.443
0.246 -
1.801 1.9494 0.667520.00 0.615 0.44 1.789 1.7753 0.574040.00 0.647 0.42 1.707 1.8682 0.625060.00 0.624 0.39 1.585 1.8014 0.588680.00 0.654 0.387 1.573 1.8885 0.6358
100.00 0.707 0.38 1.545 2.0423 0.7141120.00 0.776 0.357 1.451 2.2426 0.8076140.00 0.838 0.266 1.081 2.4226 0.8848160.00 1.056 0.461
0.389 -
1.874 3.0554 1.1169180.00 1.114 0.334 1.358 3.2237 1.1705200.00 1.21 0.391 1.589 3.5024 1.2534220.00 1.308 0.296 1.203 3.7868 1.3315240.00 1.366 0.282 1.146 3.9552 1.3750260.00 1.366 0.273 1.110 3.9552 1.3750280.00 1.479 0.167 0.679 4.2832 1.4547300.00 1.512 0.4
0.392 0.362
1.626 4.3790 1.4768320.00 1.577 0.198 0.805 4.5676 1.5190340.00 1.578 0 0.000 4.5705 1.5196360.00 1.58 0 0.000 4.5764 1.5209380.00 1.515 0.013 0.053 4.3877 1.4788400.00 1.53 0.029 0.118 4.4312 1.4887420.00 1.38 0.565 2.297 3.9958 1.3852440.00 1.482 0.79 3.211 4.2919 1.4567460.00 1.634 0.782 3.179 4.7331 1.5546480.00 1.629 0.803 3.264 4.7186 1.5515
0.377
Yx/s 2.629mu 0.0041
0.00 100.00 200.00 300.00 400.000.0000
0.2000
0.4000
0.6000
0.8000
1.0000
1.2000
1.4000
1.6000f(x) = 0.0040659327x + 0.3592744422R = 0.9565033596
Evol. BiomasaCrecimientoLinear (Crecimiento)
Axis Title
Axis Title
-
Glucosa i P0.000 1.000 0.0200.000 2.000 0.0600.013 3.000 0.1000.029 4.000 0.1400.167 5.000 0.1800.198 6.000 0.2200.266 7.000 0.2600.273 8.000 0.3000.282 9.000 0.3400.296 10.000 0.3800.334 11.000 0.4200.357 12.000 0.4600.380 13.000 0.5000.387 14.000 0.5400.390 15.000 0.5800.391 16.000 0.6200.400 17.000 0.6600.420 18.000 0.7000.440 19.000 0.7400.443 20.000 0.7800.461 21.000 0.8200.565 22.000 0.8600.782 23.000 0.9000.790 24.000 0.9400.803 25.000 0.980
0.000 0.100 0.200 0.300 0.400 0.500 0.600 0.700 0.800 0.9000.000
0.200
0.400
0.600
0.800
1.000
1.200
GPN
0.00 100.00 200.00 300.00 400.000.0000
0.2000
0.4000
0.6000
0.8000
1.0000
1.2000
1.4000
1.6000f(x) = 0.0040659327x + 0.3592744422R = 0.9565033596
Evol. BiomasaCrecimientoLinear (Crecimiento)
Axis Title
Axis Title
-
0.000 0.100 0.200 0.300 0.400 0.500 0.600 0.700 0.800 0.9000.000
0.200
0.400
0.600
0.800
1.000
1.200
GPN
-
S inicial mu Yx/s 1/mu 1/s s/mu mu/S6.517 0.00380 0.1193 263.158 0.153 1714.949 0.0005836.381 0.00360 10.4936 277.778 0.157 1772.388 0.0005642.665 0.00320 1.1206 312.500 0.375 832.9504 0.0012011.801 0.00410 1.4396 243.902 0.555 439.2227 0.002277
0.10 0.20 0.30 0.40 0.50 0.600
50100150200250300350
f(x) = - 33.4082317396x + 284.696522659R = 0.0498023569
Metodo Lineweaver-Burk
Metodo Lineweaver-BurkLinear (Metodo Lineweaver-Burk)
Axis Title
Axis Title
1.00 2.00 3.00 4.00 5.00 6.00 7.000
500
1000
1500
2000
f(x) = 266.3523409435x + 33.664828721R = 0.9862324342
Langmuir
LangmuirLinear (Langmuir)
Axis Title
Axis Title
0 0.001 0.002 0.0030.000000.000500.001000.001500.002000.002500.003000.003500.004000.00450
f(x) = 0.220982466x + 0.0034195101R = 0.2212042811
Eadie-Hofstee
Eadie-HofsteeLinear (Eadie-Hofstee)
Axis Title
Axis Title
-
1.00 2.00 3.00 4.00 5.00 6.00 7.000
500
1000
1500
2000
f(x) = 266.3523409435x + 33.664828721R = 0.9862324342
Langmuir
LangmuirLinear (Langmuir)
Axis Title
Axis Title
0 0.001 0.002 0.0030.000000.000500.001000.001500.002000.002500.003000.003500.004000.00450
f(x) = 0.220982466x + 0.0034195101R = 0.2212042811
Eadie-Hofstee
Eadie-HofsteeLinear (Eadie-Hofstee)
Axis Title
Axis Title
-
S inicial mu Yx/s 1/mu 1/s s/mu mu/S10.000 0.00380 0.0560 263.158 0.100 2631.579 0.00038
7.500 0.00360 10.4936 277.778 0.133 2083.333 0.000485.000 0.00320 1.3359 312.500 0.200 1562.5 0.000642.500 0.00410 1.4396 243.902 0.400 609.7561 0.00164
0.0000.1000.2000.3000.4000.5000.000
50.000100.000150.000200.000250.000300.000350.000
f(x) = - 95.9686975412x + 294.3280065392R = 0.1980062734
Lineweaver-burk
Lineweaver-burkLinear (Lineweaver-burk)
Axis Title
Axis Title
0 0.0005 0.001 0.0015 0.0020.000000.000500.001000.001500.002000.002500.003000.003500.004000.00450
f(x) = 0.4008522446x + 0.003360331R = 0.379285925
eadie
eadieLinear (eadie)
Axis Title
Axis Title
-
0.0000.1000.2000.3000.4000.5000.000
50.000100.000150.000200.000250.000300.000350.000
f(x) = - 95.9686975412x + 294.3280065392R = 0.1980062734
Lineweaver-burk
Lineweaver-burkLinear (Lineweaver-burk)
Axis Title
Axis Title
2.000 4.000 6.000 8.000 10.000 12.0000
500
1000
1500
2000
2500
3000
f(x) = 263.4520753102x + 75.2166238768R = 0.9768271142
Langmuir
LangmuirLinear (Langmuir)
Axis Title
Axis Title
0 0.0005 0.001 0.0015 0.0020.000000.000500.001000.001500.002000.002500.003000.003500.004000.00450
f(x) = 0.4008522446x + 0.003360331R = 0.379285925
eadie
eadieLinear (eadie)
Axis Title
Axis Title
-
2.000 4.000 6.000 8.000 10.000 12.0000
500
1000
1500
2000
2500
3000
f(x) = 263.4520753102x + 75.2166238768R = 0.9768271142
Langmuir
LangmuirLinear (Langmuir)
Axis Title
Axis Title
10gL7,5gL5gL2.5 gLajustes expajustes teor
