KVRI KR Burbot Subcommittee Meeting October 7, 2008

Nathan R. JensenUniversity of Idaho\Fish and Wildlife Departmentnjensen@uidaho.edu208.885.5734

UI Burbot Aquaculture Progress 2008

KVRI 2000

Introduction• Where we left off 2007 • 2008 Goal and

Objectives

2008 Summaries: • Observational studies• Production

Presentation outline

1 mm

Where we left off 2007

Spawning:• Expect volitional spawning to occur w/wo hormone.

Incubation:• Increase number of Imhoff cones.• Treat eggs with Iodine during water hardening.

Larval feeding:• Expand live feed production.• Feed live prey >50 days before transition.• Hand feed commercial diets and explore other diets.

Artificial pond culture:• Graduate student (MS) project.

Cryopreservation:• Continue establishing germ plasm repositories at UI.

Goal:• Produce 5,000 commercial diet

transitioned burbot.

Observations:• Observe Ovaplant affect on

spawning behavior.

• Observe sensitivity of water hardening eggs to Ovadine.

• Compare Otohime and INVE larval weaning diets.

2008 Goal, Observational studies

1. Further observe Ovaplant affect on spawning behavior

• 20 females observed:

a. 10 females given Ovaplant injections.

b. 10 females not Injected.

Objective 1 – Spawning observation

2. Observe water hardening egg sensitivity to Ovadine

• 11 spawns included in study

a. Treated eggs with 0, 25 or 50ppm Povidone Iodine

b. Determined percent live eggs at 48 hours; 3 samples per incubator

Objective 2 - Ovadine observation

3. Compare Otohime and INVE larval diets

• 4600 larvae stocked into each of four tanks after 10 weeks of live diet feeding

a. Larval weaning diets fed six weeks

b. Survival, growth, length, cannibalism compared

Objective 3 - Larval feed trial

Spawning behavior summary :

Ovaplant injected:• 100 % spawned• 0 % rest year

No Oviplant:• 80 % spawned• 20 % rest year

Spawning observation results

Spawning behavior summary:

Ovaplant injected:• 70 % volitionally spawned• 30 % spawned manually

No Oviplant:• 30 % volitionally spawned• 50 % spawned manually

Spawning observation results

Ovadine observation results

Note: No statistical analysis because not all treatments applied to all individual spawns.

Ovadine Treatment-Moyieeggs-all 11 spawns

0 ppm

25 ppm

50 ppm0

25

50

75

100

Ovidine concentration

Perc

en

t fe

rtil

izati

on

Larval feed trial results - survival

NOTE: No significant difference 31 d or 46 d post dry diet introduction; p = 0.0634 and 0.0657 respectively (n=2).

Larval feed trial results – growth

Relative growth rates:

Per day: • INVE = 0.15 mm. • Otohime = 0.13 mm.

Per month: • INVE = 4.6 mm. • Otohime = 4.0 mm.

Larval feed trial results - length

Mean (n=20) TL of larvae at theend of feed trial

INVE Otohime0

10

20

30

A

B

Diet

mm

NOTE: Larval lengths were found significantly different; p = 0.0051.

Larval feed trial - Cannibalism

Cannibals per tank:

• INVE = 23 and 24.• Otohime = 10 and 14.

Relative percent at end of trial in each tank:

• INVE = 2% and 7%.• Otohime = 1% and 2%. Cannibals were removed when observed.

Production Overview 2008

Outline:

1. Spawning

2. Incubation

3. Live feeds

4. Larval / Juvenile survival

5. Cryopreservation

Spawning 2008

Spawning results:

• 16 spawning events occurred.

• 12 events were volitional.

• 5 females had rest year.

• 21 of 25 males produced milt.

Spawning 2008

Egg collection results:

• 7.6 M eggs were collected.

• Fertilization averaged 84%;o range 38 - 99%.

• 6.7 M eggs became fertilized.

Incubation 2008

Incubation results:

• Water temperature 3-5˚ C.

• Hatches began after ~34 d;o range 28 - 41 d.

• Hatching lasted ~13 d;o range 4 - 21 d.

• 55,000 cripples were culled.

Live feeds production 2008

Larvae / Juveniles - September 2008

NOTE: September 1st ~30% were cannibals.

Cryopreservation 2008

• Milt samples were cryopreserved from all 2007 captures.o Four sets of samples were cryopreserved.

NOTE: All Moyie males captured to date are represented in cryo-storage (c/oSteve Patton; UI Biological Science Department .

NOTE: For current inventory contact UI BioSci Department .

2008 burbot semen sampling:

Research support overview 2008

UI Research: • 109,000 eggs used for egg fungus control experiment. • 30,000 feeding larvae to extensive rearing experiment. • 600 feed trained fingerlings to disease susceptibility experiments.

IDFG Research:• 56,000 feeding larvae used for extensive rearing observations.

Future research:• Submitted 3 early life developmental sets to CSU Larval fish laboratory. c/o Darrel Snyder, curator.

Implications and plans for 2009

Spawning and gamete production:1. Collect gametes from wild rather than new adults.

2. Continue use of hormone implants to promote repeat spawning.

Live feeds production:1. Rotifers: target production = 100 M per day.

2. Artemia: target production = 50 M per day.

3. Incorporate automated live feeds injection systems.

Implications and plans for 2009

Experiment with larval diets:1. INVE (Lansy / EPAC diets)2. Otohime (β and C diets)3. Skretting (Gemma micro diets)

Continue supporting UI graduate and IDFG research:1. Extensive rearing experimentation.2. Temperature related growth, survival and condition

experimentation.

There are no plans to cryopreserve milt in 2009.

Funding and Support

Support:Kootenai Tribal Fish Hatchery

BC Ministry of Environment

Idaho Department of Fish and Game

University of Idaho

KVRI

Funding :Kootenai Tribe of Idaho and The Bonneville Power Administration Project:198806400; Contracts:20490, 25349

Contact Information:Nathan R. Jensennjensen@uidaho.edu208.885.5734