Kromasil EternityXTUHPLC/HPLC columns with extended lifetime

Fit for survival‒ even under tough conditionsKromasil EternityXT UHPLC and HPLC columns are our next generation Eternity columns designed to improve efficiency and increase flexibility in your laboratory. This new family of columns is based on our state-of-the-art grafting technology, to be used for reversed-phase separations and purifications that could demand harsh conditions, fast turnaround, easy method transfer and seamless scale-up from R&D to production.

Work faster across the board With the EternityXT family of columns, you can now easily develop and validate UHPLC methods for synthetic and natural products, even under tough pH conditions; followed by seamless method transfer to HPLC for characterization and quality control; and, if required, direct scale-up for your isolation and purification needs. With our extensive assortment of slurry-packed columns, combined with the wide range of particle sizes from 1.8 µm to 10 µm, we help you improve productivity by using one stationary phase type from the same vendor across your entire company.

The EternityXT columns platformKromasil EternityXT columns are the next generation Eternity columns. These new columns are based on further development of the patent-pending Eternity state-of-the-art grafting technology, with superior column-to-column, batch-to-batch reproducibility.The high chemical stability of the EternityXT columns allows for free choice of buffers and/or the possibility of running at high temperatures.

Kromasil EternityXT stationary phase platform with C18 derivatization.

Availability of Kromasil EternityXT columns• chemistries: C18 and PhenylHexyl• particle sizes: 1.8, 2.5, 5 and 10 µm• column diameters: from 2.1 to 50 mm• column length: from 50 to 300 mm

For actual available offerings, visit our website: www.kromasil.com.

Laboratory efficiency with small particlesWhen you need to get results faster without compromises, EternityXT columns are your alternative to achieve the desired laboratory efficiency.

With EternityXT columns you can maintain separation power across all dimensions and particle sizes. Here is an illustration of faster result turnaround with maintained resolution when using shorter columns with smaller particles.

ConditionsStationary phase: Kromasil EternityXT, C18, particle sizes as in figures Flow rate: 1 ml/min, 2 ml/min, 2.8 ml/min (respectively)Column size: 4.6 x 150 mm, 4.6 x 75 mm, 4.6 x 50 mm (respectively) Temperature: 25°C Mobile phase: acetonitrile/water/formic acid [25/75/0.1] Detection: UV @ 254 nmSubstances: 1: uracil, 2: sulfathiazole, 3: sulfamerazin, 4: sulfamethoxazole

[min]

1

2

3

4

1.8 µm

0 0.6 1.2[min]

1

2

3

4

2.5 µm

0 1 2[min]

1

2

3

4

5 µm

0 2 4 6 8

Reduce costs using long-lasting columnsThe EternityXT family of columns is based on our latest advancement in stationary phase manufacturing where the columns can be exposed to extended pH conditions. Even with this wide range of operation, these columns have shown consistent and reproducible results.

Evolution of column efficiency (N) and peak asymmetry factor (As) with the number of sample injections at high and low pH.

ConditionsColumn: Kromasil EternityXT, 5 µm, C18, 4.6 x 150 mm Flow rate: 1 ml/minMobile phase, low pH: acetonitrile/20 mM potassium phosphate pH 2.5 [50/50] Temperature: ambientMobile phase, high pH: acetonitrile/10 mM ammonium carbonate pH 10.5 [70/30] Detection, low pH: UV @ 220 nmSubstances: acetophenone and toluene Detection, high pH: UV @ 210 nm

N

0

30

60

90

120

150×103

As

1.0

1.1

1.2

1.3

1.4

1.5

injections0 250 500 750 1000

N, low pHAs, low pHN, high pHAs, high pH

Choose your selectivityControlling selectivity with pH gives you extended flexibility to develop your next analytical method. EternityXT columns are the choice even for cases where harsh pH conditions can make the unique difference in your separation.

ConditionsColumn: Kromasil EternityXT, 1.8 µm, C18, 2.1 x 50 mm Substances: AI: angiotensin I, AII: angiotensin II, AIII: angiotensin IIIMobile phase, low pH: acetonitrile/water + 0.1 % TFA Mobile phase, high pH: acetonitrile/water with 0.1 % ammonium hydroxideGradient, low pH: 0 min: 14%, 10 min: 41% acetonitrile Gradient, high pH: 0 min: 5%, 10 min: 40% acetonitrileFlow rate: 0.7 ml/min Temperature: 30°C Detection, low pH: UV @ 220 nm Detection, high pH: UV @ 225 nm

Separation of angiotensinsat low and high pH[min]

AI

AII + AIIIlow pH

0 2 4 6 8[min]

AIAII

AIII

high pH

0 2 4 6 8

www.kromasil.com

The moment you adopt our Kromasil High Performance Concept, you join thousands of chromatographers who share a common goal: to achieve better separations when analyzing or isolating pharmaceuticals or other substances.

Not only will you benefit from our patented silica technology, but you gain a strong partner with a reliable track record in the field of silica products. For the past 70 years, we have pioneered new types of silica. Our long experience in the field of silica chemistry is the secret behind the development of Kromasil, and the success of our Separation Products group. Kromasil is available in bulk and in high-pressure slurry-packed columns. The development, production and marketing of Kromasil are ISO 9001 certified.

Kromasil is a brand of AkzoNobel, the largest global paint and coatings company and a major producer of specialty chemicals with headquarters in Amsterdam, the Netherlands. With 50 000 people in more than 80 countries around the world, we are committed to sustainability and delivering leading products and technologies to meet the growing demands of our fast-changing world.

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Kromasil® is a registered trademark of AkzoNobel in a number of territories in the world.

Consistent results between columns and batchesSince AkzoNobel controls the entire manufacturing process of EternityXT columns, from the initial production steps of the stationary phase to the finished packed columns, we can assure column-to-column reproducibility as well as between batches.

Comparisions of three columns showing column-to-column (A) and batch-to-batch (B) reproducibility.

ConditionsColumns: Kromasil EternityXT, 2.5 µm, C18, A: 4.6 x 100 mm, B: 2.1 x 100 mm Flow rates: A: 1.7 ml/min, B: 0.65 ml/minMobile phases: acetonitrile/water A: [70/30], B: (65/35) Temperatures: A: 25°C , B: 35°CSubstances: 1: dimethyl phthalate, 2: toluene, 3: biphenyl, 4: phenanthrene Detection: UV @ 254 nm

[min]

1

23

4B

0 1 2 3 4[min]

1

23 4

A

0 1 2 3 4 5

Alternatives for your separationsEternityXT C18 columns are commonly used when alternatives are needed to the traditional reversed-phase C18 as EternityXT can resist an extended pH range. EternityXT PhenylHexyl phase provides you with an additional alternative, especially when the analytes of interest contain an aromatic ring.

Separation of xanthines on Kromasil EternityXT PhenylHexyl

ConditionsColumn: Kromasil EternityXT, 5 µm, PhenylHexyl, 4.6 x 250 mm Flow rate: 1 ml/minMobile phase: acetonitrile/water/formic acid [40/60/0.1] Temperature: 30°C Substances: 1: theobromine, 2: 1,7-dimethylxanthine, 3: theophylline, 4: caffeine Detection: UV @ 254 nm

[min]

21

34

0 2 4 6 8 10