Kirk Basal Salt Media
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Kirk Basal salt media: 0.22 g/l ammonium tartarate; 0.2 g/l KH2PO4; 0.05 g/l MgSO4á7H2O, 0.01 g/l CaCl2 and 1 mg/lthiamine; 10ml/l of 10% Tween solution; 1.5 mM veratrylalcohol, 10 ml/l trace elements. 5 g/l glucose 200 mg/ldyestuff. pH of themedia was adjusted to 4.5 by using 2,2 dimethyl succinicacid. Trace element solution had the following composition: 0.08 g/l CuSO4á7H2O, 0.05 g/l H2MoO4, 0.07 g/lMnSO4á4H2O, 0.043 g/l ZnSO4á7H2O 0.05 g/lFe2(SO4)3.
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Transcript of Kirk Basal Salt Media
Kirk Basal salt media:
0.22 g/l ammonium tartarate; 0.2 g/l KH2PO4;0.05 g/l MgSO4á7H2O, 0.01 g/l CaCl2 and 1 mg/lthiamine;10ml/l of 10% Tween solution; 1.5 mM veratrylalcohol,10 ml/l trace elements. 5 g/l glucose 200 mg/ldyestuff. pH of themedia was adjusted to 4.5 by using 2,2 dimethyl succinicacid.
Trace element solution had the following composition:
0.08 g/l CuSO4á7H2O, 0.05 g/l H2MoO4, 0.07 g/lMnSO4á4H2O, 0.043 g/l ZnSO4á7H2O 0.05 g/lFe2(SO4)3.
