Nordic

Newsletter 1. 2014

Kem-en-tec NordicCompeteNt BioSCieNCe SiNCe 1987

New! Sense microplate Reader – No training needed

improved SDS-pAGe – the Unbreakable gel!

pCR Clever solutions – Free testpackage!

tired of plate handling? Automate with Cybio

Upgrade your eLiSA

Save money on mirus transfection reagents

Flow Cytometry Antibodies – Single, Dual and triple Color

Clever solutions for your research:

Kem-en-tec Nordic A/s Kuldyssen 10DK-2630 taastrupPhone +45 3927 1777info@kem-en-tec-nordic.com www.kem-en-tec-nordic.com

All prices are valid until 1. sept 2014. local currency exclusive VAt and ex works.

improve your NGS - recommended by illumina

KAPA Library Preparation Kits provide all of the enzymes and reaction buffers required for constructing Illumina libraries from fragmented dsDNA via the following steps:

1. End Repair

2. A-Tailing 3. Adaptor Ligation

4. Library Amplification

Reaction buffers are supplied in convenient, concentrated master mix formats comprising all of the required reaction components except oligonucleotide adaptors or PCR primers. Similarly, a single enzyme mixture is provided for each step of the library construction process, reducing the number of pipetting steps.

The library amplication module within each KAPA Library Preparation Kit contains the KAPA HiFi HotStart DNA Polymerase, a novel enzyme engineered for high fidelity and increased affinity for DNA. The high processivity of the polymerase enables efficient amplification of targets across a broad range of GC content, minimizing bias and improving the uniformity of sequence coverage. More uniform coverage enables efficient sequencing where the highest overall coverage can be achieved from the least amount of total sequence.

KAPA Library Preparation Kits are supplied with either a standard PCR library amplification module or a real-time PCR amplification module. Real-time monitoring of library amplification provides additional information required to minimize bias due to over-amplification.

Illumina Sequencing Workflow

SAMPLE PREPARATION

QUANTIFICATION

CLUSTER AMPLIFICATION

SEQUENCING

END REPAIR

A–TAILING

ADAPTOR LIGATION

LIBRARY PREPARATION

OREND-POINTAMPLIFICATION

REAL-TIMEAMPLIFICATION

SIZE SELECTION

KAPA DNA LibraryPreparation Kits

Automated library QC Fragment AnalyzerAfter dsDNA is fragmented in NGs library preparation labs typically qualify and quantify the DNA prior to sequencing runs. Conventional fragment sizing with manual gels or lab-on-chip methods, however, often creates bottle-necks for NGs labs. Not anymore.

improve Quantity/Quality Assessment Separation and quantification of NGS fragment libra-ries using the Fragment Analyzer system. Detection limits as low as 5pg/µl per fragment peak. the Fragment Analyzer is suitable for all NGs systems.

Learn more about library quant

experience the simplicity

Recommended by illumina for library and sample quality control

2 Kem-en-tec Nordic · Finland +358 9594822 · sweden/Norway +46 18 149000 · Denmark +45 3927 1777

Recommended by illumina for library quantification

Streamline work�low by rapidly quantifying and qualifying fragments for NGS runs.

NGS ANALYSIS

ADVANCED ANALYTICAL > WWW.AATI-US.COM Fragment Analyzer™ AUTOMATED CE SYSTEM

Fragment Analyzer™ Automated CE System

After dsDNA is fragmented in NGS library preparation, labs typically qualify and quantify the DNA prior to sequencing runs. Conventional fragment sizing with manual gels or lab-on-chip methods, however, often creates bottlenecks for NGS labs. Not anymore. The automated Fragment Analyzer™ allows NGS labs to accelerate throughput to 1,500 samples per day and can measure fragments as large as 15,000 base pairs, well beyond conventional methods.

Fragment Analyzer™ system. Smear analysis was performed in the 200 bp to 1000 bp range. Concentration, % of total, molarity, average size, and

Well Sample ID Range ng/ul % total nmole/L Avg. Size %CV

C1 A1 (Blue) 200 bp to 1000 bp 2.47 92.6 10.67 381 39.8

C2 A2 (Red) 200 bp to 1000 bp 3.289 93.7 16.0054 338 10.8

C3 A3 (Orange) 200 bp to 1000 bp 4.613 96.1 24.2029 314 8.1

C4 A4 (Green) 200 bp to 1000 bp 4.531 97.1 23.2067 321 15

C5 A5 (Brown) 200 bp to 1000 bp 3.033 97.8 15.7404 317 24.4

Well Sample ID Range ng/ul % total nmole/L Avg. Size %CV

A1 1 (Blue) 1000 bp to 10,000 bp 25.249 96.7 11.0346 3767 39.1

A2 2 (Red) 1000 bp to 10,000 bp 9.592 98 4.1748 3782 39.5

A3 3 (Orange) 1000 bp to 10,000 bp 16.477 93.4 6.7782 4002 44.4

A4 4 (Green) 1000 bp to 10,000 bp 9.122 98.2 3.5506 4229 41.1

A5 5 (Brown) 1000 bp to 10,000 bp 6..84 96.9 2.8506 3950 40.4

the Fragment Analyzer™ system. Smear analysis was performed in the 1000 bp to 10,000 bp range. Concentration, % of total, molarity, average

sizing range.

19000

18000

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35 100

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13000

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75 250

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Simplify your library prep

optimal library quantificationKapa Library quantification kits. High performance qPCr solution for next-generation sequencing library quantification.

Do you want to improve your NGS results?

Replace your truSeq kit today KApA Library preparation Kits provide all of the enzymes and reac tion buffers required for constructing

illumina and

ion torrent

fragment libraries.

KApA Library Quantification Kits· reduce variability in cluster density· Pool index libraries for multiplex sequencing

KApA2G Fast DNA polymeraseKAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme derived through a process of molecular evolu-tion. the Polymerase was engineered for higher proces-sivity and speed, offering significantly faster extension rates than wild-type taq poly-merase. In addition to speed, KAPA2G FAst provides higher yields and sensitivity than competitor enzymes across a broad range of targets.

KAPA2G Fast DNA Polymerase offers:

· extension times as low as 1 sec/kb

· Broad coverage of both At- and GC-rich targets

· reduction in PCr reaction time up to 70%

· High speed without compromising performance

· Availability in Hotstart and readyMix formulations

At the same price as regular taq

The first DNA polymerase evolved for extreme

speed and performanceQuicken your pCR

Receive our FRee “Clever pCR solution kit”

midori Green Advance DNA Stain1ml for 709 kr

Agarose tabletsready-to-use200 pcs for 1000 kr

DNA marker100bp100 reactions for 475 kr

Gel Cutter

50 pcs for 390 kr

the safe alter-nativ to etBR

easy as never before

improved ladder

Get your bands

Get rid of etBr!As sensitive as EtBr but non-toxic.

Just take one tablet and add 50 ml gel running buffer, the result is a 1% gel.

Now you can excise your DNA bands easily.

Kem-en-tec Nordic · info@kem-en-tec.dk · www.kem-en-tec-nordic.com 3

Containing Midori Green Advanced, ready-to-use Agarose tablets, our 100bp marker, gel cutters and Kapa2G Fast polymerase.

Latest!

New supplier

ArcticZymes!

- unique thermolabile enzymes

eg ”A’SAp” for pCR-Clean Up in

only 15 minutes!

4 Kem-en-tec Nordic · Finland +358 9594822 · sweden/Norway +46 18 149000 · Denmark +45 3927 1777

• Simple and intuitive handling with touch screen• Easy changing of tips• Serial dilution possible with special 8 channel magazine

• Quick start: no programming or plate definitions required -remember pipetting heights• Two working positions for fast liquid transfer

CyBio AG20 yeARS oF GeRmAN QUALity

AppLiCAtioNS:• Sample Preparation for PCR clean-up

• DNA extraction and purification

• Next Generation Sequencing (NGS)

• ADME and cytotoxicity assays

• 1, 8, 12, 16 and 24 channel pipetting etc

SpeCiFiCAtioNS: • 12 deck positions on two levels

• Small footprint of 650 x 450 mm

• Volume range of 500 nl - 1 ml

• Full format flexibility

Flexible compact pipetting platform for fully automated single to multichannel liquid handling

Automation- of echo, Cell Assays, Hts,

Virus screening and much more

mail/Call for Application Notes ! includes tips & tricks for setups

Call for Application

Notes!

tired of moving plates?

- or pipetting by hand?

Cybio Videos

A semi-automated 96 or 384 channel pipettor

CyBi®-SELMA

CyBi®-FeliX

From europa Bioproducts:• Liquid stable substrates, used in elIsA assays

• Streptavidin, for conjugation and coating applications

• Streptavidin conjugates, used in luminex and Affymetrix applications

• Substrates, used in bioluminescence substrates, used in clinical chemistry reagents

• Substrates, used to detect Alkaline phosphatise and peroxidase for immunoblotting applications

• trinder reagents, used in clinical chemistry reagents

We are your supplier of diagnostic raw material

Kem-en-tec Nordic · info@kem-en-tec.dk · www.kem-en-tec-nordic.com 5

Our tMB HrP enzyme substrate is easy and safe to use:• Free of harmful organic solvents• Ready-to-use solution• More signal (compared to OPD)• Up to 4 years of shelf life

Crocodile is an ideal helper in the lab for running up to 3 plates per day. And assist in other jobs in the lab involving precision dispensing, washing, OD reading and incubation of 96 well microtiter plates. Also available in version without reader at lower price! - contact us for more information.

CAtALoG # 4380A DKK SeK NoK €

1000 Ml tMB ONe 1.715,- 2.045,- 1.910,- 235,-

tAKe ADVANtAGe oF oUR SpRiNG CAmpAiGN pRiCe

155.000,- DKK 182.500,- seK 172.500,- NOK 20.600,- €

tmB - the safe choice

easy eLiSA

eLiSA upgrades

Crocodile Video

Free tmB Sample - contact peter Andersen

Free eLiSA Guide

product overview

From iQ products:AntibodiesA wide range of high quality single, dual and triple color antibody reagents in line with diagnostic guidelines in

Flow cytometry analysis.

Upgrade 2 - Automate your eLiSA A Crocodile will do the tedious eLiSA steps for you

Upgrade 1 - Use eco friendly tmB substrate

tRANSFeCtioN

NeWS

• exceptional DNA Delivery - In insect cell types including sf9, High Five™ and s2• High Baculovirus production - Ideal for baculovirus expression in insect cells • Serum compatibility - Non-liposomal, animal-origin free formulation that eliminates media change • Better Value - low reagent amounts required per transfection Free sample

of any mirus reagent

transIt®-Insect transfection reagent

High yield transient transfection and baculovirus titers in insect cells

transfectopedia™with nearly two decades of nucleic acid delivery experience, the transfection experts at Mirus Bio bring you a com-prehensive transfectopedia™ that ad-dresses various aspects of transfection.

electroporation Cuvetteswe have cuvettes for all electroporators on the market, to a competitive price

NeW!reagent Agent® Cell transfection Data-base is now available as a Universal App

Download App

No. ELEcTroporATioN Cuvettes QUANtity DKK SeK NoK All individually wrapped and sterile

EP-101 1mm Universal Fit Cuvette 50 1.299,- 1.556,- 1.407,-

EP-201 1mm Universal Fit Cuvette with Long Electrode 50 1.299,- 1.556,- 1.407,-

EP-102 2mm Universal Fit Cuvette 50 1.299,- 1.556,- 1.407,-

EP-202 2mm Universal Fit Cuvette with Long Electrode 50 1.299,- 1.556,- 1.407,-

EP-104 4mm Universal Fit Cuvette 50 1.299,- 1.556,- 1.407,-

PP-101 Disposable sterile Individually wrapped Plastic Pipettes 50 175,- 210,- 190,-

6 Kem-en-tec Nordic · Finland +358 9594822 · sweden/Norway +46 18 149000 · Denmark +45 3927 1777

Kem-en-tec Nordic · info@kem-en-tec.dk · www.kem-en-tec-nordic.com 7

order FRee RunBlue test packagetest Package: 2 gels of your choice + buffers and the 15 minute InstantBlue gel stain.

BULK oRDeR DiSCoUNt DKK SeK NoK €

1 box of gels (10 gels) 975,- 1.165,- 1.085,- 130,-

2 boxes of gels (per box) 927,- 1.107,- 1.031,- 124,-

3 boxes of gels (per box) 878,- 1.049,- 977,- 117,-

6 boxes of gels (per box) 829,- 991,- 923,- 111,-

New improved SDS-pAGe Gels

Crackfree gels

superior resolution – see bands not possible to see before

runBlue Benefits:

• colored wells for easy loading

• Neutral pH – 1 year shelf life

• Strong gel that doesn’t crack

• Fast run times (40 min)

• Gels for both invitrogen and BioRad electrophoresis cells

Download protein tool Catalog

with PC touchscreen operated software and the compact application ready microplate reader, we turn your applications into results, simply at your fingertip.

with the right tools we guarantee you can effectively focus on your work.the HiDeX Sense microplate reader is loaded with several unique innovative features to provide full flexibility at top performance.

• Five detection technologies - sensitive and fast!

• Best known technologies for each detection technology

• Absorbance spectrum read with spectrograph detector

• Luminescence with photon counting detection

• Fluorescence top and bottom with analog detection

• Fluorescence Polarization with analog detection

• Time Resolved Fluorescence with photon counting detection

• Automatic measurement height adjustment

extremely user friendly microplate reader - Hidex Sense

“We chose the Hidex Sense due to the clever integration of many applications in one reader with the very intuitive touch

screen interface.”

Assistant professor Jonas Thue Treebak from Copenhagen University

8 Kem-en-tec Nordic · info@kem-en-tec.dk · www.kem-en-tec-nordic.com

ReQUeStA Demo

Demo Video

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