Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes...
Transcript of Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes...
![Page 1: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/1.jpg)
Kefir administration reduced Kefir administration reduced progression of renal injury in STZprogression of renal injury in STZ--
diabetic ratsdiabetic rats
Cristina Stewart Bogsan
Pharmaceutical-Biochemical Technology Department
![Page 2: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/2.jpg)
OutlineOutline
• Diabetic Mellitus;
• Kefir;
• Oxidative stress;
• Aim;• Aim;
• Protocol;
• Results;
• Conclusion.
![Page 3: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/3.jpg)
Diabetes MellitusDiabetes MellitusDiabetes Mellitus (DM) is a
group of metabolic disorders thathave in common the hyperglycemia.
DM has become a seriouspublic health problem that affectspublic health problem that affectsmillions of individuals worldwide(Shaw, 2010).
30% of patients developnephropathy, the main cause ofmorbidity and mortality in diabeticpatients (Molitch et al., 2004).
Source: Shaw, 2010
![Page 4: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/4.jpg)
![Page 5: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/5.jpg)
HYPERGLYCEMIA
Increased ROS production
Impaired NO bioavailability
NF-kB Activation
Oxidative Stress in DMOxidative Stress in DM
bioavailability
Lipid peroxidation (LPO)
Endothelial dysfunction
Malandialdehyde (MPA)
Thiobarbituric acid substances (TBARS)
NF-kB Activation
Pro-inflamatorybiomarkers
Increase C-reactive protein (CRP)
![Page 6: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/6.jpg)
KefirKefir• Kefir is a fermented milk that contains a complex
symbiotic mixture of Lactic Acid Bacteria (LAB) and
Molds.
• The main microorganisms are:• The main microorganisms are:o Lactobacillus,
o Lactococcus,
o Leuconostoc,
o Streptococcus,
o Kluyveromyces,
o Saccharomyces,
o Torula.
![Page 7: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/7.jpg)
Kefir propertiesKefir properties
Kefir is known for providing benefits to human
health through its anti-inflammatory, immune-
stimulatory and antioxidant properties.
![Page 8: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/8.jpg)
AIMAIM
This study aimed at assessing the effects of Kefir on
oxidative stress in diabetic animals.
![Page 9: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/9.jpg)
STZ - diabetic induction45 mg/kg iv
Skimmed Milk
Fermentation until pH 4.6
20mg/ 100mL Kefir DA Inoculation
Kefir preparation
Stiring(1min)
Distribution in 50 mL cups
Cooling in ice bath
Storing (4 °C)
Kefir fermented milk
![Page 10: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/10.jpg)
ProtocolProtocolSTZ Kefir
5 days 24 hs
Sacrificed
24 hs 8 weeks
Kidney histology
CTLCTL CTLKCTLK DMDM DMKDMK
Kefir groups intake 1.8 mL/day by gavageWater to CTL and DM groups
Water and Chow intakeDiuresis, WeightFasting Blood toleranceCreatinine,Proteinuria, Tbars, NO
CTLCTL CTLKCTLK DMDM DMKDMK
Water and Chow intakeDiuresis, WeightFasting Blood toleranceCreatinine,Proteinuria, Tbars, NO
![Page 11: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/11.jpg)
Metabolic profile, renal function, and Metabolic profile, renal function, and oxidative stressoxidative stress
![Page 12: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/12.jpg)
Metabolic profile, renal function, and Metabolic profile, renal function, and oxidative stressoxidative stress
![Page 13: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/13.jpg)
GlycemiaGlycemia levelslevels
![Page 14: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/14.jpg)
Oral Glucose Tolerance TestOral Glucose Tolerance Test
![Page 15: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/15.jpg)
Renal functionRenal function
![Page 16: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/16.jpg)
Renal functionRenal function
![Page 17: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/17.jpg)
Oxidative StressOxidative Stress
![Page 18: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/18.jpg)
Oxidative StressOxidative Stress
![Page 19: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/19.jpg)
Inflammatory biomarkerInflammatory biomarker
![Page 20: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/20.jpg)
Kidney histologyKidney histology
![Page 21: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/21.jpg)
ConclusionConclusion
The results obtained in this study show that Kefir
treatment significantly reduced the progression of STZ-
induced hyperglycemia and oxidative stress in rats.induced hyperglycemia and oxidative stress in rats.
Kefir may play a role in slowing the metabolicchanges that contribute to DM as a non-pharmacological adjuvant .
![Page 22: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/22.jpg)
AcknowledgmentsAcknowledgments
UnifespElisa M.S. Higaa,
Giovana R. Punaroa,
Fabiane R. Maciela,
Adelson M. Rodriguesa,
Silvia S.M. Iharab,
This work was supported by:
Coordenação de
Aperfeicoamento de Pessoal
de Nivel Superior (CAPES),
Silvia S.M. Iharab,
Sergio R.R. Araujob
USPMarcelo M. Rogeroc,
Marice N. Oliveirad,
Talita R.C. Sanchese,
Lucia C. Andradee,,
Fundação de Apoio a
Universidade Federal de São
Paulo (FAP-Unifesp),
Fundação de Amparo a
Pesquisa do Estado de São
Paulo (FAPESP).
![Page 23: Kefir administration reduced progression of renal injury in STZ-- … · 2014-10-07 · Diabetes Mellitus Diabetes Mellitus (DM) is a group of metabolic disorders that haveincommonthehyperglycemia.](https://reader034.fdocuments.us/reader034/viewer/2022042909/5f3a6c05696fab5cf00c1a66/html5/thumbnails/23.jpg)
Thank youThank [email protected]