Data on file.For Research Use Only. Not for use in diagnostic procedures.

Product Summary

KAPA Mouse Genotyping KitsFrom tail to type in 1 hour!

KAPA Mouse Genotyping Kits are designed for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols. The kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed.

Benefits include:

• 15-minute extraction eliminates organic solvents, pH neutralization and centrifugation

• 45-minute amplification with high processivity and extreme speed

• Convenient master mix format with loading dye in both hot start and non-hot start formulations

Compared to the Quanta Mouse Genotyping Kit, KAPA Mouse Genotyping Kits exhibit better sensitivity, yield, and specificity in multiplex PCR. Reactions were performed with annealing at either 50°C or 60°C to demonstrate the effect of using sub-optimal annealing temperatures in genotyping assays.

Outperforms crude extraction methods and commercial kits• Increased turnaround times and specificity

• Decreased cost

Annealing at 60ºC Annealing at 50ºC

KA

PA

M 10 ng 1 ng 1:20 1:200 1:2000 1:20000 NTC 10 ng 1 ng 1:20 1:200 1:2000 1:20000 NTC

Annealing at 60ºC Annealing at 50ºC M 10 ng 1 ng 1:20 1:200 1:2000 1:20000 NTC 10 ng 1 ng 1:20 1:200 1:2000 1:20000 NTC

Qua

ntaCo

st p

er r

eact

ion

($)

KAPA MouseGenotyping Kit

Phire™ AnimalTissue Direct PCR Kit(Thermo Scientific)

REDExtract-N-Amp™Tissue PCR Kit

(Sigma)

2.50

2.00

1.50

1.00

0.50

$1.45

$2.18

$1.05

Tim

e (h

ours

)

KAPA MouseGenotyping Kit

Proteinase K +Wild-type Taq

Alkaline lysis +Wild-type Taq

ExtractionAmplificationTotal

6.0

5.0

4.0

3.0

2.0

1.0

0.0

For Research Use Only. Not for use in diagnostic procedures.KAPA is a trademark of Roche. All other product names and trademarks are the property of their respective owners.© 2020 Roche Sequencing and Life Science. All rights reserved. MC-US-06995 SS303001 A520 6/20

Published by:

Roche Sequencing and Life Science 9115 Hague Road Indianapolis, IN 46256

sequencing.roche.com

Ordering informationRoche cat. no. KAPA code Description Kit size07961766001 KK7302 KAPA Mouse Genotyping Kit 500 rxn

07961804001 KK7352 KAPA Mouse Genotyping Kit (HotStart) 500 rxn

07960921001 KK5121 KAPA2G Fast Genotyping PCR Mix 500 rxn

07961316001 KK5621 KAPA2G Fast Genotyping PCR Mix (HotStart) 500 rxn

Increased throughput, turnaround time, and reliability• PCR-ready DNA generated in as little as 15 minutes

• Minimal handling, thereby reducing the risk of sample loss or contamination

• Sufficient template for multiple assays; easily scaled to handle samples in a 96-well format

• Fast and reliable amplification of DNA fragments across a wide range of amplicon lengths and GC contents

(A) The total time required for DNA extraction and amplification was compared using the KAPA Mouse Genotyping Kit, Proteinase K and wild-type Taq, and alkaline lysis and wild-type Taq.

(B) Results for five amplicons (312 – 915 bp) generated with the KAPA Mouse Genotyping Kit (1) were compared to those obtained with two commonly used methods (2 and 3). With the KAPA Mouse Genotyping Kit, DNA lysates were prepared from mouse tails with the rapid (15 min), single-tube KAPA Express Extract system. Amplification with the KAPA2G Fast HotStart ReadyMix with dye was completed in 45 min. In contrast, DNA lysates were prepared with a ~3.5-hr Proteinase K protocol (2) or a rapid (~2.5 hr) alkaline lysis method (3). In both cases, amplification was performed with wild-type Taq (1.5 hr cycling protocol). Results obtained with the KAPA Mouse Genotyping Kit were equal or better (more specific) than those obtained with other methods, which (depending on the exact DNA extraction protocol used), may take at least four times as long, or up to 1 day to complete.

Mnxl,312 bp

IL-22,327 bp

Rosa2,360 bp

RosaI,578 bp

Aphlb,915 bp

M 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3

Cos

t per

rea

ctio

n ($

)

KAPA MouseGenotyping Kit

Phire™ AnimalTissue Direct PCR Kit(Thermo Scientific)

REDExtract-N-Amp™Tissue PCR Kit

(Sigma)

2.50

2.00

1.50

1.00

0.50

$1.45

$2.18

$1.05

Tim

e (h

ours

)

KAPA MouseGenotyping Kit

Proteinase K +Wild-type Taq

Alkaline lysis +Wild-type Taq

ExtractionAmplificationTotal

6.0

5.0

4.0

3.0

2.0

1.0

0.0

A

B