7/24/2019 Jurnal Pcr Kelompok

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Judul Jurnal: DETECTION OF ACTIVE FEMALE GENITAL TUBERCULOSIS BY

MOLECULARMETHOD

Jenis PCR  : Single tube nested e!ese t"ns#i$ti%n $%l&'e"se #("in e"#ti%n )STN RT*+CR,-

Diagnosis : Untu. di"gn%sis $en&".it tube.ul%sis $"d" .el"'in $ee'$u"n

Komponen/master mix : Untu. RT +CR  

• /0 HL #%nt"ining 1-2 HM "ntisense %ute $i'e 

• /11 HM dNT+ )e"#(,3

• /HL 415 Bu66e3

• 4-/ HL MgCl/ )/0 'M74-0 'L,3

• RN"se in(ibit% )4 U,3 /-0 U %6 MMLV e!ese t"ns#i$t"se3

• 1-0 U %6 H8*UNG )"ll +e.in*El'e,

• 9-0 HL %6 is%l"ted t%t"l RNA-Untu. 'e'ul"i sintesis #DNA unt"i $et"'" d"n .e'udi"n .e 9 %C sel"'" /

'enit untu. 'en%n".ti6."n H8* UNG-

Sel"in itu 91 HL e".si +CR 'eng"ndung :

• 0 HL 415 Bu66e3

• ; HL MgCl/3 0 U  TTH  DNA $%li'e"se )se'u" +e.in*El'e,3

• 132 HM "s" lu" $i'e d"n +CR .el"s "i )Sig'" C%- USA,

digun"."n untu. 'el".u."n %ute +CR-

Cara membaca hasil : +e'b"#""n ("sil 'enggun"."n "g"%se gel ele.t%6%esis untu. $e'b"#""n

("sil "n"lisis +CR-Fig. 1 A

  M NC1 PC S1 NC2 S2 S3 S4 S5 S6 S7 S8 S9

Fig.1 B

 M NC1 PC S10 NC2 S11 S12 S13 S14 S15 S16 S17 

G 8el%'$%. < /B

• Anis" Nu Ins"ni

• 8("is'" Auli" Ul6"

• S%n&" Nil" M"&""

• ="nd" Retn% =i "ti

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