JUAN PABLO DE RIVERO VACCARI, Ph.D. University of Miami Miller School of Medicine

Curriculum Vitae 1. DATE: January 5, 2017 I. PERSONAL 2. Name: Juan Pablo de Rivero Vaccari, Ph.D. 3. Home Phone: Private 4. Office Phone: (305) 243-7113 5. Home Address: Private 6. Current Academic Rank: Research Assistant Professor 7. Primary Department: The Miami Project to Cure Paralysis

Neurological Surgery 8. Secondary Appointments: N/A 9. Citizenship: United States 10. Visa Type (if non-citizen): N/A II. HIGHER EDUCATION 11. Institutional:

2007 Ph.D. Physiology and Biophysics University of Miami Academic Excellence Miami, FL. 2004 B.S. Biology Florida International University Summa Cum Laude Miami, FL. 2001 A.S. Vision Care Technology/Opticianry Miami Dade College Highest Honors Miami, FL. 1999 A.S. General Science E.P. Nunez Community College Presidential Honors Chalmette, LA.

12. Non-Institutional:

April 2016 Understanding Biomarker Science: From Molecules to Images The Harvard Clinical and Translational Science Center – Harvard Catalyst. Boston, MA.

August 2009 Summer School on Quantitative and Computation Immunology. Duke University Laboratory of Computational Immunology and The Santa Fe Institute Santa Fe, NM.

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July 2008 Molecular Mechanisms of Human Disease: Injury, Inflammation, and Tissue Repair. ASIP 2008 Summer Academy.

American Society for Investigative Pathology Bethesda, MD. 13. Certification, licensure:

2012 – Present Working with Swine in Research Settings Collaborative Institutional Training Initiative University of Miami 2012 – Present Working with Mice in Research Collaborative Institutional Training Initiative University of Miami 2010 – Present Aseptic Surgery Collaborative Institutional Training Initiative University of Miami 2006 – Present CITI Course in the Protection of Human Research Subjects Collaborative Institutional Training Initiative University of Miami 2005 – Present Occupational Health and Safety for Lab Animal Users University of Miami 2005 – Present Post-Procedure Care of Mice and Rats in Research:

Reducing Pain and Distress University of Miami 2005 – Present Working with the Institutional Animal Care and Use

Committees (IACUC) University of Miami 2005 – Present Working with the Laboratory Rat University of Miami 2000 – Present American Board of Opticianry Certified (ABOC) American Board of Opticianry 2000 – Present National Contact Lens Examiner Certified (NCLEC) National Contact Lens Examiners 2010 – 2012 National Contact Lens Examiners Certified. Aproved Speaker to provide Continuing Education.

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2005 – 2010 Animal Welfare Policies and Procedures University of Miami

III. EXPERIENCE 14. Academic:

11/10 - Present Research Assistant Professor

Department of Neurological Surgery The Miami Project to Cure Paralysis University of Miami Miller School of Medicine Miami, FL. 2009 – 2010 Senior Research Associate I Department of Neurological Surgery The Miami Project to Cure Paralysis University of Miami Miller School of Medicine Miami, FL. 2007 – 2009 Postdoctoral Associate W. Dalton Dietrich, III, Ph.D., Laboratory The Miami Project to Cure Paralysis University of Miami Miller School of Medicine Miami, FL. 08/04 – 06/07 Graduate Student Robert W. Keane, Ph.D., Laboratory Department of Physiology and Biophysics University of Miami Miller School of Medicine Miami, FL. 2001 – 2002 Adjunct Faculty Vision Care Technology/Opticianry Miami Dade College Miami, FL. 15. Hospital Appointments: N/A 16. Non-Academic 2009 - Present InflamaCORE, LLC. Co-founder and Managing Member Trademark: MARK: InflamaCORE

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SERIAL NUMBER: 85384184 Registration number: 4,440,663 Registration date: November 26, 2013

For International Class 005: Current identification: Diagnostic kits comprised of reagents, antibodies, buffers, and physiological solutions, sold as a unit

Intellectual Property:

1. Modulating Inflammasome Activity and Inflammation in the Central Nervous System. US Patent Application: 15/337,265 (Provisional) CON of 14/219,857

2. Innate Immune Proteins as Biomarkers for Central Nervous System Injury. • US Patent Application: 15/214,868 (Provisional) • International Patent Application: PCT/US2013/024941 (Pending) • European Patent Application:

EP13746979.7 (Pending)

3. Modulating Inflammasome Activity to treat Inflammatory Conditions. US Patent: 9/512,209 (Granted)

US Patent Application: 14/219,857 (Other version) CON of 12/182,886

4. Modulating Inflammasome Activity and Inflammation in

Central Nervous System Injury. US Patent: 8/685,400 (Granted) US Patent Application: 12/182,886 (Other version)

Funding: 5R42NS086274-03 (08/01/2016 – 07/31/2017)

NIH (NINDS) R42 $ 248,609 Therapeutic Neutralization of the Inflammasome After Spinal Cord Injury

4R42NS086274-012 (08/15/2015 – 07/31/2016)

NIH (NINDS) R42 $1,054,411 Therapeutic Neutralization of the Inflammasome After Spinal

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Cord Injury 1R42NS086274-01 (04/01/2014 – 03/31/2015)

NIH (NINDS) R42 $191,553 Therapeutic Neutralization of the Inflammasome After Spinal Cord Injury

Coulter Foundation Funds $265,000 This corresponds to funding used in the development of a biologic to target the inflammasome. UM SAC 2014-6 (10/01/2013 - 09/30/2014) SAC Bridge Award $25,000 (total costs) Therapeutic Neutralization of the Inflammasome after Spinal Cord Injury

2001 – 2002 Ophthalmic Assistant Orlando A. Galindez, M.D. Ophthalmologist Miami, FL. 2000 – 2001 Student-Worker Miami Dade College Miami, FL. 17. Military: N/A IV. PUBLICATIONS 18. Books:

1. Book chapter: de Rivero Vaccari JP and de Rivero Vaccari JC. Biomarkers in

Disease: Methods, Discoveries and Applications. General Methods and their Applications, edited by Victor Preedy and Vinood B. Patel. Inflammasome Proteins as Biomarkers of Injury and Disease. Springer 2015. 1207-1228.

2. de Rivero Vaccari Juan Carlos, Ph.D., de Rivero Vaccari, Juan Pablo, Ph.D., A.

Alan Sobel. O.D. For the Contact Lens Clinician. © 2011. ISBN 978-0-615-40884-2. 3. de Rivero Vaccari Juan Carlos, Ph.D., de Rivero Vaccari, Juan Pablo, Ph.D., A.

Alan Sobel. O.D. Contact Lens Review: 1,000 Questions and Answers. © 2010. ISBN 978-0-615-43173-4.

19. Scientific articles in refereed journals (H-index: 21)

First author publications: 12 articles (includes 2 first co-authorships) Corresponding author publications: 7 articles Review articles: 3 articles

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Average citations/year: 176 citations/year (since 2006) 389 citations/year (2014-2016)

1. Hummler JK, Dapaah-siakwan F, Vaidya R, Zambrano R, Luo S, Kerr N, de Rivero Vaccari JP, Keane RW, Dietrich WD, Bancalari E, Young KC, Wu S. Inhibition of Rac1 Signaling Down-regulates Inflammasome Activation and Attenuates Lung Injury in Neonatal Rats Exposed to Hyperoxia. Neonatology. 111 (3), 280-288.

2. Elmasry S, Asfour S, de Rivero Vaccari JP, Travascio F. A Computational Model for Investigating the Effects of Changes in Bioavailability of Insulin-Like Growth Factor-1 on the Homeostasis of the Intervertebral Disc. Computers in Biology and Medicine. 2016 Sep 22;78:126-137.

3. Brand FJ, Forouzandeh M, Kaur H, Travascio F, de Rivero Vaccari JP. Acidification

Changes Affect the Inflammasome in Human Nucleus Pulposus Cells. J Inflammation. 2016; 13(1): 29.

4. Lavesque SA, Pare A, Mailhot B, Bellver-Landete HK, Lecuyer MA, Alvarez JI, Prat A, de Rivero Vaccari JP, Keane RW, Lacroix S. Myeloid cell transmigration across the CNS vasculature triggers Il-1β-driven neuroinflammation during autoimmune encephalomyelitis in mice. J Exp Med. 2016 May 2. JOURNAL COVER

5. de Rivero Vaccari JP, Patel HH, Brand FJ III, Perez-Pinzon MA, Bramlett HM and Raval AP. Estrogen receptor beta signaling alters cellular inflammasomes activity after global cerebral ischemia in reproductively senescence female rats. J Neurochem. 2015 Oct 22.

6. Brand FJ III, de Rivero Vaccari JC, Mejias, NH, Alonso OF and de Rivero Vaccari JP. RIG-I Contributes to the Innate Immune Response After Cerebral Ischemia. J Inflammation. 2015, 12:52.

7. Asfour S, Elmasry S, de Rivero Vaccari JP, Travascio F. Effects of Tobacco

Smoking on the Degeneration of the Intervertebral Disc: a Finite Element Study. PLoS ONE. Aug 24; 10(8):e0136137.

8. de Rivero Vaccari JP, Dietrich DW, Keane RW. Therapeutics Targeting the

Inflammasome After Central Nervous System Injury. Translational Research. 2015 May 12. pii: S1931-5244(15)00145-0.

9. de Rivero Vaccari JP, Brand F III, Adamczak SE, Lee SW, Barcena, JP, Wang MY,

Bullock MR, Dietrich DW, Keane RW. Exosome-Mediated Inflammasome Signaling After Central Nervous System Injury. J Neurochem. 2015 Jan 27.

10. de Rivero Vaccari JC, Brand F III, Berti AF, Alonso OF, Bullock MR, de Rivero Vaccari JP. Mincle Signaling in the Innate Immune Response after Traumatic Brain Injury. J Neurotrauma 2015 Feb 15;32(4):228-36

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11. Asfour S, Travascio F, Elmasry S, de Rivero Vaccari JP. A Computational Analysis

on the Implications of Age-Related Changes in the Expression of Cellular Signals on the Role of IGF-1 in Intervertebral Disc Homeostasis. J Biomech. 2015 Jan 21;48(2):332-9

12. Ibrahim E, Castle SM, Aballa TC, Keane RW, de Rivero Vaccari JP, Lynne CM,

Brackett NL. Neutralization of Apoptosis Associated-Speck Like Protein Containing a CARD (ASC) Improves Sperm Motility in Men With Spinal Cord Injury. Hum Reprod. 2014 Nov;29(11):2368-73.

13. Kigerl KA, de Rivero Vaccari JP, Dietrich DW, Popovich PG, Keane RW. Pattern

Recognition Receptors and Central Nervous System Repair. Experimental Neurology. 2014 Aug;258:5-16.

14. Dumas A, Amiable N, de Rivero Vaccari JP, Chae JJ, Keane RW, Lacroix S,

Vallieres A. The Inflammasome Pyrin Contributes to Pertussis Toxin-Induced IL-1β Synthesis, Neutrophil Intravascular Crawling and Autoimmune Encephalomyelitis. PLoS Pathog. 2014 May 29;10(5):e1004150.

15. de Rivero Vaccari JP, Brand FJ, Sedaghat C, W. Dalton Dietrich, Robert W. Keane.

RIG-1 Receptor Expression in the Pathology of Alzheimer’s Disease. J. Neuroinflammation. 2014 Apr 2;11(1):67.

16. de Rivero Vaccari JP, W. Dalton Dietrich, Robert W. Keane. Activation and Regulation of Cellular Inflammasomes: Gaps in our Knowledge for Central Nervous System Injury. J Cereb Blood Flow and Metab. 2014 Mar;34(3):369-75. JOURNAL COVER

17. Adamczak SE, de Rivero Vaccari JP, Dale GA, Brand F III, Nonner D, Bullock R, Dahl G, W. Dalton Dietrich, Robert W. Keane Pyroptotic Neuronal Cell Death Mediated by the AIM2 Inflammasome. J Cereb Blood Flow and Metab. 2014 Apr;34(4):621-9.

18. Stojadinovic O, Minkiewicz J, Sawaya A, Bourne JW, Torzilli P, de Rivero Vaccari

JP, Dietrich WD, Keane RW, Tomic-Canic M. Deep Tissue Injury in Development of Pressure Ulcers: A Decrease of Inflammasome Activation and Changes in Human Skin Morphology in Response to Aging and Mechanical Load. PLoS One. 2013 Aug 14;8(8):e69223.

19. Minkiewicz J, de Rivero Vaccari JP, Keane RW. Human Astrocytes Express a Novel NLRP2 Inflammasome Activated by ATP. Glia. 2013 Jul;61(7):1113-21.

20. Adamczak SE, Dale GA, de Rivero Vaccari JP, Bullock R, Dietrich DW, Keane RW.

Inflammasome Proteins in Cerebrospinal Fluid of Brain Injured Patients are Potential Biomarkers of Functional Outcome. J Neurosurg. 2012 Dec;117(6):1119-25.

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21. Ibrahim E, Zhang X, Aballa TC, de Rivero Vaccari JP, Keane RW, Dietrich WD,

Lynne CM, Brackett NL. Involvement of the Inflammasome in Abnormal Semen Quality of Men With Spinal Cord Injury. Fertil Steril. 2012 Oct 3. pii: S0015-0282(12)02182-6.

22. Tomura S, de Rivero Vaccari JP, Keane RW, Bramlett HM, Dietrich DW. Effects of therapeutic hypothermia on inflammasome signaling after traumatic brain injury. J Cereb Blood Flow Metab. 2012 Oct;32(10):1939-47.

23. de Rivero Vaccari JP, Sawaya ME, Brand FJ, Nusbaum BP, Bramlett HM, Dietrich

DW, Keane RW. Inflammasome Regulation in Androgenetic Alopecia. J Dermatologic Surgery. 2012 Jul;38(7 Pt 1):1033-9.

24. de Rivero Vaccari JP,* Bastien D,* Yurcisin GH, Pineau I, Dietrich WD, DeKonic Y,

Keane RW, Lacroix S. P2X4 Receptor Regulates Inflammasome Activation Following Spinal Cord Injury. J. Neurosci 2012 Feb 29;32(9):3058-66. * First coauthors.

25. de Rivero Vaccari JP, Minkiewicz J, Wang X, de Rivero Vaccari, JC, German R,

Marcillo AE, Dietrich WD, Keane RW. Astrogliosis Involves Activation of Rig-Like Signaling in the Innate Immune Response After Spinal Cord Injury. Glia. 2012 Mar;60(3):414-21.

26. Mawhinney LJ, de Rivero Vaccari JP, Alonso O, Jimenez CA, Furones C, Moreno

WJ, Lewis MC, Dietrich WD, Bramlett HM. Isoflurane/nitrous oxide anesthesia induces increases in NMDA receptor subunit NR2B protein expression in the aged rat brain. Isoflurane anesthesia induces increases in NMDA receptor subunit NR2B protein expression in the aged rat brain. Brain Research. 2012 Jan 11;1431:23-34.

27. Daftarian P, Chowdhury R, Wei C, Ames P, King AD, de Rivero Vaccari JP,

Price J, Zuchner P, Reiser J, Lemmon V, Keane RW. In vivo electroporation and non-protein based screening assays to identify antibodies against native protein conformations. Hybridoma. October 2011, 30(5): 409-418.

28. Nadeau S, Filali M, Zhang J, Kerr BJ, Rivest S, Soulet D, Iwakura Y, de Rivero Vaccari JP, Keane RW, Lacroix S. Functional Recovery after Peripheral Nerve Injury is Dependent on the Pro-Inflammatory Cytokines IL-1{beta} and TNF: Implications for Neuropathic Pain. J Neurosci. 2011 Aug 31;31(35):12533-12542.

29. Wang X, de Rivero Vaccari JP, Wang H, Diaz P, German R, Marcillo A, Keane RW.

Activation of the Nuclear Factor E2-Related Factor 2/Antioxidant Response Element Pathway is Neuroprotective Following Spinal Cord Injury. J Neurotrauma. 2012 Mar 20;29(5):936-45. JOURNAL COVER

30. Lotocki G, de Rivero Vaccari JP, Alonso O, Sanchez Molano J, Nixon R, Safavi P,

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Dietrich WD, Bramlett HM. Oligodendrocyte vulnerability following traumatic brain injury in rats. Neurosci Lett. 2011 Jul 25;499(3):143-8.

31. Lotocki G, de Rivero Vaccari JP, Alonso O, Sanchez Molano J, Nixon R, Dietrich WD, Bramlett HM. Oligodendrocyte Vulnerability Following Traumatic Brain Injury in Rats: Effect of Moderate Hypothermia. Therapeutic Hypothermia and Temperature Management. March 2011, 1(1): 43-51.

32. de Rivero Vaccari JP, Marcillo A, Nonner D, Dietrich WD, Keane RW. Neuroprotective effects of bone morphogenetic protein 7 (BMP7) treatment after spinal cord injury. Neurosci Lett. 2009 Nov 20;465(3):226-9.

33. Lotocki G, de Rivero Vaccari JP, Perez ER, Sanchez-Molano J, Furones-Alonso O,

Bramlett HM, Dietrich WD. Alterations in Blood-Brain Barrier Permeability to Large and Small Molecules and Leukocyte Accumulation after Traumatic Brain Injury: Effects of Post-Traumatic Hypothermia. J Neurotrauma. 2009 Jul;26(7):1123-34.

34. Silverman WR, de Rivero Vaccari JP, Locovei S, Qiu F, Carlsson SK, Scemes E, Keane RW, Dahl G. The pannexin 1 channel activates the inflammasome in neurons and astrocytes. J Biol Chem. 2009 Jul 3; 284(27):18143-51.

35. de Rivero Vaccari JP, Lotocki G, Alonso OF, Bramlett HM, Dietrich WD, Keane RW. Therapeutic neutralization of the NLRP1 inflammasome reduces the innate immune response and improves histopathology after traumatic brain injury. J Cereb Blood Flow Metab. 2009 Jul;29(7):1251-61.

36. Abulafia DP, de Rivero Vaccari JP, Lozano JD, Lotocki G, Keane RW, Dietrich WD. Inhibition of the inflammasome complex reduces the inflammatory response after thromboembolic stroke in mice. J Cereb Blood Flow Metab. 2009 Mar;29(3):534-44.

37. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. A molecular platform in neurons regulates inflammation after spinal cord injury. J Neurosci. 2008 Mar 26;28(13):3404-14.

38. Lotocki G, de Rivero Vaccari JP, Perez ER, Alonso OF, Curbelo K, Keane RW, Dietrich WD. Therapeutic hypothermia modulates TNFR1 signaling in the traumatized brain via early transient activation of the JNK pathway and suppression of XIAP cleavage. Eur J Neurosci. 2006 Oct;24(8):2283-90.

20. Abstracts

1. Elmasry S, Asfour S, de Rivero Vaccari JP, Travascio F. A Computational Analysis

on the Effects of Reduced Bioavailability of Insulin-Like Growth Factor-1 on the Homeostasis of the Intervertebral Disc. Orthopedic Research Society (ORS) 2017 Annual Meeting. San Diego, CA (In Press).

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2. de Rivero Vaccari JP. Therapeutic Targeting of the Inflammasome. International Congress on Neuroimmunology and Therapeutics. December 1-2, 2016. Atlanta, GA. Page 38.

3. de Rivero Vaccari JP. Inflammasome Regulation in the Aging Brain. International Congress on Neuroimmunology and Therapeutics. December 1-2, 2016. Atlanta, GA. Page 30.

4. Keane RW and de Rivero Vaccari JP. IC100: A Novel Therapeutic to Central Nervous System Injury and Disease. Life Sciences Summit, November 2-3, 2016, New York, NY.

5. Kerr NA, Kaur H, Zambrano R, de Rivero Vaccari JP, Liebl DJ, Wu S, Bancalari E,

Keane RW, Dietrich WD. The role of inflammasome signaling in Traumatic Brain Injury-Induced Acute Lung Injury. June 2016. Neurotrauma 2016. Lexington, KY.

6. Elmasry S, Asfour S, de Rivero Vaccari JP, Travascio F. Smoking and Smoking

Cessation: Implications on the degeneration of the intervertebral disc. Summer Biomechanics, Bioengineering and Biotransport Conference 2016. June 2016. National Harbour, MD.

7. Asfour S, de Rivero Vaccari JP, Travascio F. A theoretical examination of potential strategies for enhancing cellular anabolism in the extracellular matrix of the intervertebral disc. Summer Biomechanics, Bioengineering and Biotransport Conference 2016. June 2016. National Harbour, MD.

8. Dapaah-siakwan F, Zambrano R, Hummler J, Vaidya R, Ahmad N, de Rivero

Vaccari JP, Keane RW, Dietrich WD, Bancalari E, Young K, Wu S. Inhibition of Rac-1 Signaling Suppresses Hyperoxia-induced Inflammasome and Lung Injury in Neonatal Rats. Pediatric Academic Society (PAS) Annual Meeting. April 2016. Baltimore, MD.

9. Brand FJ, Travascio F, de Rivero Vaccari JP. Effects of pH changes on inflammasome activation in the intervertebral disc. Orthopedic Research Society (ORS) 2016 Annual Meeting. Orlando, FL. March 2016.

10. Kerr NA, Valencia M, Kaur H, Gervorac B, de Rivero Vaccari JP, Conner GE, Dietrich WD, Keane RW. Exosomes from Traumatic Brain Injury subjects activate the inflammasome in human bronchial epithelial cells. Inflammation: Causes, Prevention and Cures. January 2016. Miami, FL.

11. d'Adesky ND, de Rivero Vaccari JP, Bhattachary P, Bramlett HM, Perez-Pinzon

MA, Raval AP. Estrogen receptor beta regulates inflammasome activation in the hippocampus of female rats.Inflammation: Causes, Prevention and Cures. January 2016. Miami, FL.

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12. S. Lee S, Gajavelli S, de Rivero Vaccari JP, Spurlock M, Rivera K, Kaplan M, Kaur H, Shear D, Tortella F, Dietrich WD, Keane RW. The inflammasome is activated in the cortex of rats following penetrating ballistic brain injury. Inflammation: Causes, Prevention and Cures. January 2016. Miami, FL.

13. Yeguez A, Lucas J, Diaz A, Bhattacharya P, d'Adesky ND, de Rivero Vaccari JP,

Raval AP. Sexual dimorphisms in hippocampal inflammsome activation. Inflammation: Causes, Prevention and Cures. January 2016. Miami, FL.

14. de Rivero Vaccari JP. Targeting the innate immune response to improve outcomes after CNS injury. International Congress on Neuroimmunology and Therapeutics. July 20-22, 2015 San Francisco, CA. Page 56.

15. Raval AP, Patel HH, Brand III FJ, Bramlett HM, de Rivero Vaccari JP. Estrogen

Receptor Beta Regulates Inflammasome Activation in the Hippocampus of Female Rats. Brain 2015. June 2015. Vancouver, Canada.

16. Elmasry S, Asfour S, de Rivero Vaccari JP, Travascio F. A Quantitative Evaluation

of the Role of Cell Senescence in Intervertebral Disc Degeneration. Summer Biomechanics, Bioengineering and Biotransport Conference 2015. June 2015, Snowbird Resort, Utah.

17. Ibrahim E, Aballa T, de Rivero Vaccari, JP, Keane RW, Dietrich WD, Lynne C, Brackeett N. Oral Probenecid Improves Sperm Motility in Men with Spinal Cord Injury. American Urological Association (AUA) Annual Meeting. May 2015.

18. de Rivero Vaccari JP, Brand III FJ, Travascio F, de Rivero Vaccari JC. RIG-I

signaling in the innate immune response after stroke. American Association of Immunologists 2015. New Orleans, LA May 2015.

19. Frank J. Brand III, Travascio F, de Rivero Vaccari JP. Effects of Nicotine on Inflammasome Activation in Human Nucleus Pulposus and Annulus Fibrosus Cells. Orthopedic Research Society (ORS) 2015 Annual Meeting. Las Vegas, NV. March 2015.

20. Shestopalov VI, Dvoriantchikova G and de Rivero Vaccari JP. Ganglion cell loss in retinal ischemia is facilitated by the inflammasome. International Vision Research Congress. San Francisco 2014. July 2014.

21. de Rivero Vaccari JP. Inflammasome activation in Spinal Cord Injury. FASEB Summer Conference on Translational Neuroimmunology: From Mechanisms to Therapeutics July 13-18, 2014. Big Sky, Montana. July 2014.

22. Lee SW, Gajavelli S, de Rivero Vaccari JP, Brand III F, Spurlock M, Shear D, Tortella FC, Bullock MR, Keane RW. The Inflammasome is Activated in the Cortex of Rats Following Penetrating Ballistic Brain Injury. National Neurotrauma Symposium. San Francisco 2014. J Neurotrauma. June 2014.

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23. Keane RW, de Rivero Vaccari JP. Inflammasomes in the Central Nervous System,

International Neurotrauma Symposium 2014, Budapest, Hungary, March 22, 2014.

24. de Rivero Vaccari JP, Brand F, Berti AF, de Rivero Vaccari JC. Mincle signaling contributes to the inflammatory response after traumatic brain injury. American Association of Immunologists 2013. Honolulu, Hawaii 2013.

25. Ibrahim E, Zhang X, Aballa T, de Rivero Vaccari JP, Keane RW, Dietrich WD, Lynne C, Brackett N. Inflammasome inactivation improves sperm motility in men with spinal cord injury. American Urological Association annual meeting 2013.

26. Castle S, Ibrahim E, Zhang X, de Rivero Vaccari JP, Aballa T, Brackett N, Lynne C. Pannexin-1 Is Elevated In Semen Of Men With Spinal Cord Injury Compared To Healthy Controls. Southeastern Section of the American Urological Association (SESAUA) 2013. March 2013.

27. de Rivero Vaccari JP, Brand F, Sedaghat C, Mash D, Dietrich WD, Keane RW. Rig-1 signaling is involved in the pathogenesis of Alzheimer’s disease. Neuroscience November 2012. New Orleans, LA.

28. Bastien D, de Rivero Vaccari JP, Keane RW and Lacroix S. Involvement of the IL-1 system in neutrophil and proinflammatory monocyte recruitment and functional recovery after spinal cord injury. The Neutrophil in Immunity, 1st International Symposium, Québec, QC, Canada.

29. Lévesque SA, de Rivero Vaccari JP, Keane RW and Lacroix S. Deficiency in IL-1 signaling protects from experimental autoimmune encephalomyelitis (EAE): tracking down the inflammasome involved. 2012. Neuroinflammation Training Program Symposium, 7th annual meeting, Montreal, QC, Canada.

30. de Rivero Vaccari JP, Lotocki G, Adamczack S, Wang MY, Norenberg MD, Dietrich WD, Keane RW. NLRP1 Inflammasome Protein Expression is Incresed in the Cerebrospinal Fluid of Patients After Spinal Cord Injury. Neurotrauma 2012. J. Neurotrauma July 2012.

31. Minkiewicz J, de Rivero Vaccari JP, Keane RW. A Novel NLRP2 Inflammasome is Expressed in Human Astrocytes is Activated by Extracellular ATP. Neurotrauma 2012. J. Neurotrauma July 2012.

32. Tomura S, de Rivero Vaccari JP, Keane RW, Alonso O, Bramlett HM, Dietrich WD. Effects of therapeutic hypothermia on inflammasome signaling after traumatic brain injury in rats. Neurotrauma 2012. J. Neurotrauma July 2012.

33. Adamczak S, de Rivero Vaccari JP, Dale G, Nonner D, Dietrich WD, Keane RW. Pyroptosis is a novel neuronal cell death mechanism induced by host nucleic acids

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released after traumatic brain injury, National Neurotrauma Symposium, Phoenix, AZ, 2012.

34. Adamczak S, Dale G, de Rivero Vaccari JP, Bullock MR, Dietrich WD, Keane RW. Inflammasome proteins in cerebrospinal fluid of brain injured patients are potential biomarkers of functional outcome, National Neurotrauma Symposium, Phoenix, AZ, 2012.

35. Stojadinovic O, J Minkiewicz, A Sawaya, de Rivero Vaccari JP, Keane RW, Tomic-Canic M. Differential activation of the inflammasome in loaded skin of young and elderly individuals. Society for Investigative Dermatology Annual Meeting. May 2012. Raleigh, NC.

36. Minkiewicz J, de Rivero Vaccari JP, Keane RW. Human astrocytes express a novel NLRP2 inflammasome complex activated by extracellular ATP. American Association of Immunologists 2012, Boston, MA.

37. de Rivero Vaccari JP, Brand F, Sedaghat C, Mash D, Dietrich WD, Keane RW. Role of RIG-1 signaling in the Pathogenesis of Alzheimer’s Disease. American Association of Immunologists 2012. Boston, MA. May 2012.

38. Shestopalov VI, Dvoriantchikova G, de Rivero Vaccari JP, Ivanov DV, Slepak VZ. Inflammasome Activation Facilitates Interleukin-1β Release and Ganglion Cell Loss Following Retinal Ischemia-Reperfusion. ARVO, The Association for Research in Vision and Opthalmology 2012. Fort Lauderdale, FL. May 2012.

39. Ibrahim E, Zhang X, Aballa TC, de Rivero Vaccari JP, Keane RW, Dietrich WD,

Lynne CM, Brackett NL. Possible role of the inflammasome in abnormal semen quality of men with spinal cord injury American Urological Association Annual Meeting. Atlanta. May 2012.

40. Minkiewicz J, Sawaya A, de Rivero Vaccari JP, Keane RW, Tomic Canic M, Stojadinovic S. Biomechanics And Age In Pressure Ulcers: Differential Activation of The Inflammasome. Wound Repair and Regeneration Volume 20 Number 2 March-April 2012. p. A31 Wound Healing Society 2012. Atlanta, GA.

41. Minkiewicz J, Sawaya A, de Rivero Vaccari JP, Keane RW, Tomic Canic M, Stojadinovic S. Biomechanics And Age In Pressure Ulcers: Differential Activation of The Inflammasome.

42. Sedaghat C, de Rivero Vaccari JP. Rig-1 Signaling in the Pathogenesis of Alzheimer's Disease. 2012 Undergraduate Research, Creativity and Innovation Forum. University of Miami Whitten University Center. April 11th 2012. Page 121.

43. Keane RW and de Rivero Vaccari JP. NLRP1 inflammasome activation and CNS injury. American Society for Neurochemistry Annual Meeting. Baltimore, MD. March

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2012.

44. Minkiewicz J, de Rivero Vaccari JP, Keane RW. The NLRP2 Inflammasome: A Novel Inflammatory Platform Expressed in Human Glia. 14th Annual Biomedical and Comparative Immunology Symposium. Modesto Maidique Campus, Florida International University, March 1-2nd 2012.

45. Minkiewicz J, de Rivero Vaccari JP, Keane RW. Human Astrocytes Express a Novel NLRP2 Inflammasome Complex Activated by ATP. 38th Eastern-Atlantic Student Research Forum. University of Miami. Miami, FL. February 22-25, 2012.

46. de Rivero Vaccari JP, Minkiewicz J, Wang X, German R, Marcillo AE, Dietrich WD, Keane RW. RIG-Like Receptor Signaling Modulates Innate Immunity and Astrocyte Activation Following Spinal Cord Injury. Neurotrauma 2011. J. Neurotrauma June 2011.

47. Adamczak S, de Rivero Vaccari JP, Dietrich WD Keane RW. dsDNA Induces An Innate Immune Response In Cortical Neurons. Neurotrauma 2011. J. Neurotrauma June 2011.

48. Minkiewicz J de Rivero Vaccari JP, Keane RW. The NLRP2 Inflammasome in Astrocytes Contributes to Innate Immunity Following CNS Injury. Neurotrauma 2011. J. Neurotrauma June 2011.

49. Tomura S, de Rivero Vaccari JP, Keane RW, Alonso O, Bramlett HM, Dietrich WD. The Effect of Therapeutic Hypothermia on The NLRP1 Inflammasome Signaling After Traumatic Brain Injury. Neurotrauma 2011. J. Neurotrauma June 2011.

50. de Rivero Vaccari JP, Minkiewicz J, Wang X, German R, Marcillo AE, Dietrich WD, Keane RW. RIG-LIKE Receptor Signaling Modulates the Innate Immune Response and Astrocyte Activation After Injury to the Spinal Cord. J Immunology. May 2011.

51. Keane RW, Dietrich WD, de Rivero Vaccari JP (2011) Mechanisms underlying cell death after brain injury. International Neurotrauma Symposium, Shanghai, China, J. Neurotrauma 28: A-1-A-96, A-18, O53.

52. Wang, X, de Rivero Vaccari JP, Wang H, Keane RW (2011) Sulfurophane suppresses ATP-induced inflammation via the NRF2/ARE pathway in primary neurons. International Neurotrauma Symposium, Shanghai, China. J. Neurotrauma 28:A-l-A-96, A-5, O9

53. de Rivero Vaccari JP, Yurcisin GH, Dietrich WD, De Konic Y, Bastien D, Lacroix S, Keane RW. P2X4 Receptor Regulates Inflammasome Activation Following Injury to the Spinal Cord. Society for Neuroscience Annual Meeting. San Diego, CA 2010. November 2010.

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54. de Rivero Vaccari JP, Yurcisin GH, Dietrich WD, Bastien D, Lacroix S, Keane RW. P2X4 Receptor Regulates Inflammasome Activation Following Spinal Cord Injury. National Neurotrauma Symposium. Las Vegas, NV. J Neurotrauma. May 2010, A46.

55. Bacik SE, de Rivero Vaccari JP, Yurcisin GH, Furones C, Dietrich WD, Keane RW. The IPAF inflammasome is present and upregulated after traumatic brain injury. National Neurotrauma Symposium. Las Vegas, NV. J Neurotrauma. May 2010, A46.

56. Mawhinney, Lana Jones, de Rivero Vaccari, JP, Alonso, OF, Jimenez, CA, Lewis, MC, & Bramlett, HM. (February 2010) Isoflurane Increases Acute NR2B Expression Associated with Spatial Learning Deficits in Aged Rats. Eastern-Atlantic Student Research Forum 2010. Miami, FL.

57. de Rivero Vaccari JP, Lotocki G, Marcillo A, Alonso O, Bramlett HM, Dietrich WD, Keane RW. (February 2010). Targeting the inflammasome as a therapy to improve outcomes after injury to the central nervous system. Poster presented at the 13th International Conference on Lymphocyte Activation and Immune Regulation: Inflammasome and Inflammation. Newport Beach, CA.

58. Mawhinney, Lana Jones, de Rivero Vaccari, JP, Alonso, OF, Jimenez, CA, Lewis, MC, & Bramlett, HM. (November 2009) Isoflurane induced spatial learning deficits are linked to an elevation in NR2B subunit of the NMDA receptor in a subgroup of aged rats. Poster presented at 18th Annual Neuroscience Research Day University of Miami, Miami FL.

59. Mawhinney, Lana Jones, Lewis MC, Nevo I, de Rivero Vaccari JP, Bramlett HM. (October 2009). Isoflurane Effects in Aged Rats and the Mechanism of Elderly Postoperative Cognitive Dysfunction. Annual Meeting of the American Society of Anesthesiologists 2009.

60. Lotocki G, de Rivero Vaccari JP, Perez ER, Sanchez-Molano J, Furones-Alonso O, Bramlett HM, Dietrich WD. Alterations in Blood-Brain Barrier Permeability to Large and Small Molecules and Leukocyte Accumulation after Traumatic Brain Injury: Effects of Post-Traumatic Hypothermia. Journal of Neurotrauma. July 2009, 26(7): 1123-1134. 27th Annual National Neurotrauma Symposium.

61. Dahl G, Silverman WR, de Rivero Vaccari JP, Locovei S, Qiu F, Carlsson SK, Scemes E, Keane RW. (July 2009). The pannexin 1 channel activates the inflammasome in neurons and astrocytes. International Gap Junction conference 2009.

62. de Rivero Vaccari JP, Lotocki G, Dietrich WD, Keane RW. (May 2009). The Inflammasome as a Therapeutic Target to Improve Outcomes After Injury to the Central Nervous System. Immunology 2009. Annual American Association of Immunologists Meeting.

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63. Jones, LC, de Rivero Vaccari JP, Alonso, OF, Lewis, MC, Bramlett H (2009) The acute effects on of Isoflurane treatment on Aged Rats. Eastern Atlantic Student Research Forum.

64. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Alonso O, Bramlett H, Dietrich WD, Keane RW. (November 2008). Neuronal NLRP1 Inflammasome Contributes to Regional Immunity after Central Nervous System Injury. Neuroscience 2008. Annual Society for Neuroscience Meeting.

65. Jones L, de Rivero Vaccari JP, Alonso OF, Lewis MX, Bramlett HM. Aging and general anesthesia: cognitive deficits and changes in MAPK proteins as a result of isoflurane exposure in aged rats. Program N0.592.8, 2008 Neuroscience Meeting Planner, Washington DC. Society for Neuroscience 2008.

66. Lewis, MC, Jones, LC, de Rivero Vaccari JP, Alonso, OF, Nevo, I, Bramlett H. (2008) POCD and MAPK: Cognitive Deficits related to changes in MAPK proteins as a result of Isoflurane Exposure. Poster presentation at the 21st International Congress of the Israel Society of Anesthesiologists.

67. Jones, LC, de Rivero Vaccari JP, Alonso, OF, Lewis, MC, Bramlett H (2009) The acute effects on of Isoflurane treatment on Aged Rats. Eastern Atlantic Student Research Forum, University of Miami Miller School of Medicine, Oral presentation

68. Jones L, Lewis MC, Nevo I, de Rivero Vaccari JP, Bramlett HM. (October 2008). Isoflurane Effects in Aged Rats and the Mechanism of Elderly Postoperative Cognitive Dysfunction. Annual Meeting of the American Society of Anesthesiologists 2008. Orlando, FL.

69. Silverman WR, de Rivero Vaccari JP, Locovei S, Qiu F, Carlsson SK, Scemes, E, Keane RW, Dahl G (2008) The pannexin 1 channel activates the inflammasome in neurons and astrocytes. 48th Annual American Society of Cell Biology, San Francisco, CA, Dec. 2008.

70. de Rivero Vaccari JP, Lotocki G, Alonso O, Bramlett H, Dietrich WD, Keane RW. (July 2008). The NALP1 Inflammasome in Cortical Neurons Regulates Inflammation Following Traumatic Brain Injury. 26th Annual National Neurotrauma Symposium. (Page 891).

71. Lotocki G, de Rivero Vaccari JP, Perez ER, Sanchez-Molano J, Alonso OF, Dietrich WD (2008) Alterations in Blood-Brain Barrier permeability to large and small molecules and leukocyte accumulation after traumatic brain injury: effects of therapeutic hypothermia. 26d Annual Neurotrauma Society Symposium, Orlando, FL, J. Neurotrauma.

72. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (June 2008). Spinal Cord Injury Activates the NALP1 Inflammasome In Neurons and ASC

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Neutralization Improves Histopathological and Functional Outcomes. 14th Annual Kentucky Spinal Cord and Head Injury Research Symposium.

73. Jones, LC, de Rivero Vaccari, JP, Lewis, MC, Nevo, I, Bramlett, H (2008) Biochemical and Behavioral Variations in Aged Rats Related to the Inhalation-Anesthetic Isoflurane. Presentions include: The Eastern Atlantic Student Research Forum (March 2008), Neuroscience Research Day 2008; both held at the University of Miami Miller School of Medicine, Miami, FL, Also presented at the Research and Creativity Forum, University of Miami, Miami FL (April 2008)

74. Abulafia DP, de Rivero Vaccari JP, Lozano JD, Ragan TJ, Keane RW, Dietrich WD (May 2007) Thromboembolic stroke activates the formation of the inflammasome complex. Immunology 2007, 94th Meeting of the American Association of Immunologists. Miami Beach, FL.

75. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (November 2007). Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and ASC Neutralization Improves Histopathological and Functional Outcomes. Neuroscience 2007. Annual Society for Neuroscience Meeting.

76. de Rivero Vaccari JP, Dietrich WD, Marcillo AE, Keane RW. (February 2006). Characterization of Inflammatory Caspase-Activating Platforms Following Spinal Cord Injury. 32ndAnnual Eastern-Atlantic Student Research Forum. Translational Medicine: From Bench to Bedside. Page 28.

77. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (July 2007). Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and ASC Neutralization Improves Histopathological and Behavioral Outcomes. 25th Annual National Neurotrauma Symposium. Page 1286.

78. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (April 2007). Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and ASC Neutralization Improves Histopathological and Behavioral Outcomes. 48th Annual National Student Research Forum. Page 93.

79. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (April 2007). Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and ASC Neutralization Improves Histopathological and Behavioral Outcomes. 2007 Citizens Board Research & Creativity Forum. Page 18.

80. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (February 2007). Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and ASC Neutralization Improves Histopathological Outcome. 33rdAnnual Eastern-Atlantic Student Research Forum. Page 40.

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81. de Rivero Vaccari JP, Dietrich WD, Marcillo AE, Keane RW. (April 2006). Characterization Of Inflammatory Caspase-Activating Platforms Following Spinal Cord Injury. 47th Annual National Student Research Forum. Page 55.

82. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. (April 2006). Spinal Cord Injury Activates The NALP1 Inflammasome Complex. 2006 Citizens Board Research & Creativity Forum. Page 137.

21. Other works accepted for publication

1. Myeloid cell transmigration across the CNS vasculature triggers Il-1β-driven neuroinflammation during autoimmune encephalomyelitis in mice. Lavesque SA, Pare A, Mailhot B, Bellver-Landete HK, Lecuyer MA, Alvarez JI, Prat A, de Rivero Vaccari JP, Keane RW, Lacroix S. J Exp Med. 2016 May 2. JOURNAL COVER

2. de Rivero Vaccari JP, W. Dalton Dietrich, Robert W. Keane. Mechanism of inflammasome activation. JOURNAL COVER. J Cereb Blood Flow and Metab. 2014 Mar 34(3).

3. de Rivero Vaccari JP, Dietrich DW, Keane RW. Focusing on Inflammasome and Brain Inflammation to Treat and Diagnose TBI. The Challenge! Brain Injury Association of America. Summer 2013. 7(3) Page 27.

4. Wang X, de Rivero Vaccari JP, Wang H, Diaz P, German R, Marcillo A, Keane RW. Nrf2 immunoreactivity increases after spinal cord injury (SCI) in motor neurons and astrocytes of the spinal cord. JOURNAL COVER. J Neurotrauma. 2012 Mar 20;29(5).

5. de Rivero Vaccari JP; de Rivero Vaccari JC. Keratoconus and Inflammation. EyeWitness. Quarterly Publication of the Contact Lens Society of America. Spring 2010, 21-25. ABO/NCLE Approved for 1 NCLE continuing education credit.

Doctoral Dissertation 2007 Regulation of the NALP1 Inflammasome in Neurons: A Therapeutic Target for

Spinal Cord Injury. Robert W. Keane, Ph.D., Thesis Advisor University of Miami V. PROFESSIONAL

22. Funded Research Performed Active 5R42NS086274-03 (PI: Keane) 08/01/2016 – 07/31/2017 NIH (NINDS) R42 $ 248,609 (total costs) 4.8 cal mos

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Therapeutic Neutralization of the Inflammasome After Spinal Cord Injury The goal of this study is to humanize a monoclonal antibody against the human ASC protein. Role: Co-investigator 2RO1 NS 042133-11 (PI: Dietrich) 07/01/2016 - 06/30/2021 1.2 cal mos NIH (NINDS) Importance of Temperature on the Inflammatory and Microvascular Consequences of Mild TBI The goal of this proposal is to clarify the cellular and molecular mechanisms underlying the harmful consequences of hyperthermic mild TBI and concussion. Role: Co-investigator 1R01NS094522-01 (PI: Brambilla) 09/01/2015 – 08/31/2020 0.6 cal mos NIH (NINDS) $1,678,905 (total costs) Molecular mechanisms of oligodendroglial TNFR2 protective function: a new therapeutic target in neuro-immune disease The goal of this proposal is to determine how TNFR2 in oligodendrocytes is protective in the central nervous system. Role: Co-investigator Inactive 4R42NS086274-02 (PI: Keane) 08/15/2015 – 07/31/2016 NIH (NINDS) $1,054,411 (total costs) 4.8 cal mos Therapeutic Neutralization of the Inflammasome After Spinal Cord Injury The goal of this study is to humanize a monoclonal antibody against the human ASC protein. Role: Co-investigator 12SDG11970010 (PI: de Rivero Vaccari) 07/01/2012 - 06/30/2016 American Heart Association $308,000 (total costs) Activation of Rig-like Receptor Signaling after Focal Cerebral Ischemia This study is designed to identify the role of RLR signaling in the inflammatory innate immune response after stroke. Role: Principal Investigator Coulter Foundation Funds $265,000.00 This corresponds to funding used in the development of the biologic to target the inflammasome. Role: Co-PI CB1-1101-1 (PI: de Rivero Vaccari) Christopher & Dana Reeve Foundation $25,000 (total costs) This corresponds to a gift from the foundation in support of spinal cord injury research in Dr. de Rivero Vaccari’s laboratory. Role: Principal Investigator

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1R42NS086274-01 (PI: Keane) 04/01/2014 – 08/31/2015 NIH (NINDS) $191,553 (total costs) Therapeutic Neutralization of the Inflammasome After Spinal Cord Injury The goal of this study is to humanize a monoclonal antibody against the human ASC protein. Role: Co-investigator UM SAC 2014-6 (PI: Keane) 10/01/2013 - 09/30/2014 SAC Bridge Award $25,000 (total costs) Therapeutic Neutralization of the Inflammasome after Spinal Cord Injury To humanize a monoclonal antibody against the human ASC protein. Role: Co-investigator 224598 (PI: Brackett) 07/01/2012 - 06/30/2014 Craig T. Neilsen Foundation $136,364 (total costs) Improving Reproductive Function in Men with Spinal Cord Injury (SCI) To test anti-inflammasome function in men with spinal cord injury. Role: Co-investigator 221346 (PI: Keane) 06/01/2012 - 06/30/2014 Craig T. Neilsen Foundation $136,364 (total costs) Inflammasome Regulation after Spinal Cord Injury To test anti-ASC as a therapy to improve outcomes after SCI in swine. Role: Co-investigator

23. Editorial Responsibilities 2017 - BMC Anesthesiology, ad hoc reviewer 2016 - CNS & Neurological Disorders-Drug Targets, ad hoc reviewer 2016 - International Journal of Molecular Sciences, ad hoc reviewer 2016 - Frontiers in Immunology, ad hoc reviewer 2016 - Oxidative Medicine and Cellular Longevity, ad hoc reviewer 2016 - BMC Musculoskeletal Disorders, ad hoc reviewer 2016 - Cells, ad hoc reviewer 2016 - BMC Neuroscience, ad hoc reviewer 2016 - Mediators of Inflammation, ad hoc reviewer 2015 - Frontiers in Molecular Neuroscience, Review Editor 2015 - Recent Patents on Anti-Infective Drug Discovery, ad hoc reviewer 2015 - Translational Stroke Research, ad hoc reviewer 2015 - Molecules, ad hoc reviewer 2015 - Patient Preference and Adherence, ad hoc reviewer 2014 - PLOS ONE, ad hoc reviewer 2014 - Journal of Inflammation Research, ad hoc reviewer 2014 - Journal of Parkinson’s and Alzheimer’s diseases, ad hoc reviewer 2014 - Revista Investigación Clínica (J of Clinical Investigation), ad hoc

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reviewer 2013 - Acta Neuropathologica, ad hoc reviewer 2013 - Neuroscience Letters, ad hoc reviewer 2013 - CND Neuroscience and Therapeutics, ad hoc reviewer 2013- Molecular and Cellular Biochemistry, ad hoc reviewer 2013 - Journal of Therapeutic Hypothermia and Temperature

Management, ad hoc reviewer 2012 - Journal of The European Academy of Dermatology and Venereology, ad hoc reviewer 2012 - Journal of Neuroscience, ad hoc reviewer 2011 - Journal of Visualized Experiments (JoVE), ad hoc reviewer 2010 - Journal of Neurotrauma, ad hoc reviewer 2015 Journal of Inflammation research, consulting editor 2015 Journal of Neurology and Psychology, editorial board member 2014 e-Book: Frontiers in Clinical Drug Research - Alzheimer Disorders.

Edited by prof. Atta-Ur-Rahman, FRS. Chapter reviewer. 24. Professional and Honorary Organizations

2011 – Present American Heart Association, Professional member 2000 – Present National Contact Lens Examiners, member 2000 – Present American Board of Opticianry, member 2015 – 2016 Orthopaedic Research Society, member 2009 – 2015 Contact Lens Society of America, member 2009 – 2015 American Association of Immunologists, member 2003 – 2014 Society for Neuroscience, member 2013 – 2014 International Society for Cerebral Blood Flow and Metabolism 2011 – 2013 Sigma Xi, The Scientific Research Society, member 2011 – 2012 International Brain Injury Association, member 2008 – 2009 American Society for Investigative Pathology, member 2007 – 2011 National Neurotrauma Society, member 2006 – 2007 New York Academy of Sciences, member 2005 – 2007 American Association for the Advancement of Science, member 2004 Phi Beta Kappa 2003 Phi Kappa Phi 2003 – 2005 American Society of Biochemistry and Molecular Biology, member 2001 – 2004 Fellow of the National Academy of Opticianry, member 2000 Phi Theta Kappa

25. Honors and Awards

Post-graduate 2016 American Heart Association/American Stroke Association Research

Leaders Academy 2016. San Antonio (Inaugural edition). 2012 American Association of Immunologists’ Minority Scientist Travel

22

Award funded by the FASEB MARC Program 2011 Sigma Xi, The Scientific Research Society 2009 Travel scholarship to the Summer School on Quantitative and

Computation Immunology. Duke University Laboratory of Computational Immunology and The Santa Fe Institute

2009 The American Association of Immunologists’ Trainee Abstract Award 2008 Travel award from the National on Neurological Disorders and Stroke

(NINDS) to the 14th Annual Kentucky Spinal Cord and Head Injury Research Symposium

Graduate 2007 Award of Academic Merit for Academic Excellence 2007 SfN Chapters Graduate Student Travel Award to the 37th annual

meeting of the Society for Neuroscience. Eli Lilly & Co. 2007 The Oslerian Award for Translational Research. 48th Annual National

Student Research Forum 2007 NSRF Overall Poster Award, 1st Place. 48th Annual National Student

Research Forum 2007 UTMB Neuroscience and Cell Biology Poster Award. 48th Annual

National Student Research Forum 2007 First Place in the category of Neuroscience at the Citizens Board

Research & Creativity Forum. University of Miami 2007 Travel Award from the Medical Faculty Association Margaret Whelan

Graduate Student Scholarship Fund 2007 Travel Award to the National Student Research Forum. American

Medical Association Foundation 2007 Best University of Miami Miller School of Medicine Presentation at the

33rd Annual Eastern - Atlantic Student Research Forum. American Medical Association Foundation

2006 Second Place in the category of Biomedical Sciences at the Citizens Board Research & Creativity Forum. University of Miami

2006 Travel Award to the National Student Research Forum. Medical Association Foundation

2006 Best Overall Basic Science Presentation at the 32ndAnnual Eastern-Atlantic Student Research Forum. American Medical Association Foundation

2004 – 2007 Medical School Graduate Tuition Scholarship. University of Miami Miller School of Medicine

2004 Graduate Fellowship. University of Miami Miller School of Medicine. Department of Physiology and Biophysics

Undergraduate 2004 Summa cum laude 2004 Outstanding Academic Achievement in Biological Sciences

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2004 PHI BETA KAPPA Honor Society 2003 Honorable mention for Spanish open essay: La Utopía Detrás De La

Ejecución De La Pena De Muerte. FIU Essay Contest 2003 The National Dean’s List. 26thAnnual Edition 2003 Dean’s List (spring & fall) 2003 The Honor Society of PHI KAPPA PHI 2002 Dean’s List (spring & fall) 2001 Dean’s List (fall) 2001 Beverly Myers Student Papers Award for Dry Eye – The Whole Picture 2001 Graduated with Highest Honors 2001 Board of Trustees Scholarship 2001 Outstanding Clinical Excellence 2001 Outstanding Academic Achievement 2001 Dean’s List (spring) 2000 Auxiliary to Health South Doctor’s Scholarship 2000 The National Dean’s List. 23rdAnnual Edition 2000 Graduated with Presidential Honors 2000 Honor’s Scholarship 2000 PHI THETA KAPPA International Honor Society 2000 Dean’s List (spring & fall) 1999 Dean’s List (fall) 1999 Recognition given for service to the Metropolitan New Orleans

community by participating in A.A.C.C Broadening Horizons Service Learning Project

1999 The National Dean’s List. 22ndAnnual Edition 1999 President’s List 1999 Chancellor’s List 1999 Who’s Who among Students in American Junior Colleges 1998 United States Achievement Academy’s All American Scholar 1998 The National Dean’s List. 21stAnnual Edition 1998 President’s List (spring & fall) 1997 Honor’s List

26. Post-doctoral Fellowship

2007 - 2009 Post-doctoral Associate W. Dalton Dietrich, III, Ph.D., Laboratory The Miami Project to Cure Paralysis Department of Neurological Surgery University of Miami Miller School of Medicine Miami, FL.

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27. Other Professional Activities Panel Memberships 2014 - 2016 American Heart Association, IRG grant reviewer. 2016 Brain & BrainPET 2017, abstract reviewer. 2016 National Science Centre (Narodowe Centrum Nauki (NCN)),

Poland, grant reviewer 2016 21st International Association of Gerontology and Geriatrics (IAGG)

World Congress of Gerontology and Geriatric, abstract reviewer. 2016 American Heart Association Scientific Sessions 2016, abstract

reviewer. 2015 Neurological Foundation of New Zealand, grant reviewer. 2014 Focus group with Hispanic/Latino researchers for new and early-

stage investigators organized by the NIH and the CSR to examine the peer review process, participant.

2012 NIH Clinical and Translational Science Institutional (CTSI), grant reviewer (University of Miami).

2012 University of Missouri Spinal Cord Injuries Research Program (UM SCIRP), grant reviewer (University of Missouri).

2012 New Neuroscience Faculty Conference (NIMH, NINDS). Cold Spring Harbor Laboratory, participant.

2011 – 2012 Eastern Atlantic Student Research Forum, judge 2008 – 2012 Research & Creativity Forum. University of Miami, judge 2009 16th Annual Florida-Georgia Louis Stokes Alliance for Minority

Participation (FGLSAMP) Expo, judge 2001 – Present Advisory Committee Vision Care Technology/Opticianry. Miami

Dade College, member. Trainees Supervised As Faculty 2016 Camilla Martinez. Student Volunteer 2016 Sam Abbassi, Volunteer 2015 Harmanpreet Kaur, M.D. Volunteer 2015 Michael W. Kaplan. M.D. Student Volunteer 2015 - 2016 Nancy H. Mejias. Student Volunteer 2014 Nadine S. Ahmed. Rotating student, Ph.D. program 2014 Stephanie W. Lee. Rotating student M.D., Ph.D. program 2013 - 2015 Mahtab Forouzandeh. Student volunteer. 2013 Britnee McDole. Student Volunteer. 2011 - 2012 Christina Sedaghat. Miami Project Summer Research Program,

Honor’s Thesis student. 2011 Hernando Santacoloma. Student volunteer. 2011 Marica Daniels. Students Training in Research (STIR) Program

2011 Aldo Berti, M.D. Medical School Research Rotation, Ponce

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School of Medicine, Puerto Rico. 2011 Satoshi Tomura, M.D., Ph.D. As Postdoctoral Fellow 2010 Blaise Berry

2010 Xiaoliang Wang, M.D., Ph.D. 2009 Stephanie Bacik, Ph.D. 2009 Joshua Vogt, M.D. 2009 Julia Minkiewicz, Ph.D. 2009 Gordon Dale 2008 Steven Carlsson 2008 Aldo F. Berti, M.D 2008 Andrew Ross, M.D. As Graduate Student 2007 David Molnar, Ph.D. 2007 Yang Liu, Ph.D. 2006 Yenisel Cruz-Almeida, Ph.D. 2006 Andrew Sawaya, M.S. 2005 Nidza M. Torres. Lectureships National and International Conferences 2016 Inflammasome Regulation in the Aging Brain. International Congress on

Neuroimmunology and Therapeutics. Atlanta, GA (12/01/2016) 2015 Targeting the innate immune response to improve outcomes after CNS

injury. International Congress on Neuroimmunology and Therapeutics. San Francisco, CA (07/19/2015)

2012 Rig1 is involved in the pathogenesis of Alzheimer’s Disease. Alzheimer’s

Disease: Neuroinflammation II. Theme C: Disorders of the Nervous System. Neuroscience 2012. Ernerst M Morial Convention Center. New Orleans, LA (10/15/2012)

2010 P2X4 Regulates Inflammasome Activation Following Injury to the Spinal

Cord. Neuroinflammation and CNS Injury Nanosymposium. Disorders of the Nervous System. Annual Meeting Neuroscience 2010 organized by the Society for Neuroscience. San Diego Convention Center. San Diego, CA (11/15/2010)

2009 The Inflammasome as a Therapeutic Target to Improve Outcomes After

Injury to the Central Nervous System. Session: NODs and the Inflammasome. Washington State Convention and Trade Center, Seattle,

26

Washington. Awarded with The American Association of Immunologists Trainee Abstract Award (05/12/2009)

2008 Neuronal NLRP1 Inflammasome Contributes to Regional Immunity after

Central Nervous System Injury. Session: Neurotoxicity and Neuroinflammation II at the Annual Meeting Neuroscience 2008. Society for Neuroscience. The Walter E. Washington Convention Center. Washington, DC. (11/19/2008)

2007 Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and

ASC Neutralization Improves Histopathological and Functional Outcomes Session: Multiple Approaches to Spinal Cord Injury. Annual Meeting Neuroscience 2007 organized by the Society for Neuroscience. San Diego Convention Center. San Diego, California. Awarded with SfN Chapters Graduate Student Travel Award supported by Eli Lilly & Co. (11/07/2007)

2007 Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and

ASC Neutralization Improves Histopathological and Functional Outcomes. Open Communications (SCI). 25th Annual National Neurotrauma Society Symposium. Westin Crown Center. Kansas City, MO. (07/30/2007)

2007 Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and

ASC Neutralization Improves Histopathological Outcome. 33rdAnnual Eastern-Atlantic Student Research Forum. American Medical Association Foundation. University of Miami Miller School of Medicine. Miami, FL. Awarded with Best University of Miami Miller School of Medicine Presentation Award (02/23/2007)

2006 Spinal Cord Injury Activates the NALP1 Inflammasome Complex.

47thAnnual National Student Research Forum. American Medical Association Foundation. University Of Texas Medical Branch. Galveston, TX. (05/28/2006)

2006 Characterization of the NALP1 Inflammasome Following Spinal Cord

Injury. 32ndAnnual Eastern-Atlantic Student Research Forum. American Medical Association Foundation. University of Miami Miller School of Medicine. Miami, FL. Awarded with: Best Overall Basic Science Presentation Award (02/23/2006)

University of Miami 2016 Mechanisms of Inflammasome Activation. Neurological Surgery Grand

Rounds. University of Miami Miller School of Medicine. Miami, FL. (03/03/2016)

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2015 Mechanisms of Inflammasome Activation. Dermatology Grand Rounds. University of Miami Miller School of Medicine. Miami, FL. (10/21/2015)

2012 Regulation of Pattern Recognition Receptors in CNS Injury and Disease.

The Miami Project to Cure Paralysis Seminar Series. University of Miami Miller School of Medicine. Miami, FL. (09/12/2012)

2012 Regulation of the Innate Immune Response by Pattern Recognition

Receptors after CNS Injury. Interdisciplinary Stem Cell Institute Grand Rounds. University of Miami Miller School of Medicine. Miami, FL. (03/12/2012)

2011 The Innate Immune Response in CNS Injury and Disease. Multiple

Sclerosis Center Seminar. University of Miami, Department of Neurology. Miami, FL. (10/14/2011)

2011 Pattern Recognition Receptors as Biomarkers of Alzheimer’s Disease.

McKnight Center for Age-Related Memory Loss lecture. University of Miami, Department of Neurology. Miami, FL. (09/21/2011)

2011 Regulation of the Innate Immune Response by Pattern Recognition

Receptors after Spinal Cord Injury. Department of Neurological Surgery Grand Rounds. University of Miami Miller School of Medicine. Miami, FL. (09/01/2011)

2011 Regulation of Pattern Recognition Receptors after Spinal Cord Injury.

Rheumatology Department Seminar. University of Miami Miller School of Medicine. Miami, FL. (02/04/2011)

2010 RIG-like Receptor Activation Following CNS Injury and Disease.

Department of Physiology and Biophysics Seminar. University of Miami Miller School of Medicine. Miami, FL. (10/11/2010)

2009 Inflammasome Activation After Central Nervous System Trauma.

Department of Physiology and Biophysics Seminar. University of Miami Miller School of Medicine. Miami, FL. (04/20/2009)

2008 Neuronal NLRP1 Inflammasome Contributes to Innate Immunity after

Central Nervous System Injury. Diabetes Research Institute Seminar Series. University of Miami Miller School of Medicine. Miami, FL. (12/01/2008).

2007 Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and

ASC Neutralization Improves Histopathological and Functional Outcomes. The Miami Project to Cure Paralysis Seminar Series. University of Miami Miller School of Medicine. Miami, FL. (09/12/2007)

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2007 Spinal Cord Injury Activates the NALP1 Inflammasome in Neurons and

ASC Neutralization Improves Histopathological and Behavioral Outcomes. Dissertation Defense. University of Miami Miller School of Medicine. Miami, FL. (06/08/2007)

2006 Characterization of Inflammatory Caspase-Activating Platforms Following

Spinal Cord Injury. Thesis Proposal Defense & Examination for Ph.D. Candidacy. University of Miami Miller School of Medicine. Miami, FL. (01/09/2006)

2005 Inflammatory Caspases in Spinal Cord Injury. Student Summer Seminar Series. University of Miami Miller School of Medicine. Miami, FL. (08/05/2005)

Entrepreneurship and Others 2016 Therapeutic Targeting of the Inflammasome. International Congress on

Neuroimmunology and Therapeutics. Atlanta, GA (12/02/2016)

2014 Translating the Inflammasome. FASEB Summer Conference on Translational Neuroimmunology: From Mechanisms to Therapeutics. Big Sky, Montana (07/17/2014)

2014 University of Miami - Career Symposium PANELIST. Research

Entrepreneurship: Moving Science from the Academic Lab to the World of Commerce. Student Activities Center. University of Miami. Miami, FL. (02/12/2014)

2012 Therapeutic and Diagnostic Interventions Targeting The Innate Immune

Response In Central Nervous System Injury and Disease. Enterprise Development Corporation Biotech Conference 2012. Florida International University. Miami, FL. (05/09/2012)

2010 Inflammation and the Brain. Brain Awareness Day 2010. Miami Museum

of Science. (03/20/2010) Patents and Copyrights

Patents

1. Methods of Modulating Inflammasome Activity and Inflammation in the Central

Nervous System Robert W. Keane, Ph.D., W. Dalton Dietrich, Ph.D., Juan Pablo de Rivero Vaccari, Ph.D., Helen M. Bramlet, Ph.D. US Patent Application: 15/337,265 (Provisional)

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CON of 14/219,857 2. Innate Immune Proteins as Biomarkers for Central Nervous System Injury.

Robert W. Keane, Ph.D., W. Dalton Dietrich, Ph.D., Juan Pablo de Rivero Vaccari, Ph.D., Stephanie Adamczack, Ross Bullock, M.D., Ph.D., Michael Y. Wang, M.D., Allan Levi, M.D., Ph.D. US Application Number: 15/214,868 (Provisional) International application number PCT/US2013/024941 (Pending)

European application number EP13746979.7 (Pending)

3. Modulating Inflammasome Activity to treat Inflammatory Conditions. Robert W. Keane, Ph.D., W. Dalton Dietrich, Ph.D., Juan Pablo de Rivero Vaccari, Ph.D., Helen M. Bramlet, Ph.D. US Patent: 9/512,209 (Granted)

US Patent Application: 14/219,857 (Other version) CON of 12/182,886

4. Modulating Inflammasome Activity and Inflammation in Central Nervous System Injury.

Robert W. Keane, Ph.D., W. Dalton Dietrich, Ph.D., Juan Pablo de Rivero Vaccari, Ph.D., Helen M. Bramlet, Ph.D. US Patent Application number: 12/182,886 (Other version) US Patent Application number: 8/685,400 (Granted)

5. Innate Immune Proteins as Biomarkers for Central Nervous System Injury. Robert W. Keane, Ph.D., W. Dalton Dietrich, Ph.D., Juan Pablo de Rivero Vaccari, Ph.D., Stephanie Adamczack, Ross Bullock, M.D., Ph.D., Michael Y. Wang, M.D., Allan Levi, M.D., Ph.D. US Application Number: 14/376,383 (Abandoned)

6. Compositions, methods and kits for detecting and treating Alzheimer’s disease. Robert W. Keane, Ph.D., W. Dalton Dietrich, Ph.D., Juan Pablo de Rivero Vaccari, Ph.D. Deborah Mash, Ph.D.

International application number PCT/US11/51570 (Abandoned)

7. Compositions, Kits and Methods for Determining Efficacy of a Therapeutic Agent And Treating Hair And Skin Diseases.

Robert W. Keane, Ph.D., W. Dalton Dietrich, Ph.D., Juan Pablo de Rivero Vaccari, Ph.D., Helen M. Bramlet, Ph.D.. Mary Sawaya, M.D., Ph.D. International application number PCT/US2011/026778 (Abandoned)

Copyrights 1. For the Contact Lens Clinician © Copyright: Juan Carlos de Rivero Vaccari,

Ph.D., Juan Pablo de Rivero Vaccari, Ph.D., Alan A. Sobel, O.D. 2011.

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2. CONTACT LENS REVIEW 1,000 Questions and Answers© Copyright: Juan Carlos de Rivero Vaccari, Ph.D., Juan Pablo de Rivero Vaccari, Ph.D., Alan A. Sobel, O.D. 2010. TXU001686271 (08-10-10).

3. Regulation of the NALP1 inflammasome in Neurons: A Therapeutic Target for Spinal Cord Injury©. Copyright: Juan Pablo de Rivero Vaccari. 2008. TX 6-654-366 - 01/30/2009.

4. Clinic Form 2003 ©. Copyright: Juan Carlos de Rivero Vaccari, Ph.D. and Juan Pablo de Rivero Vaccari, Ph.D. 2003. TXu1-146-297 – 07/11/2003.

VI. TEACHING 28. Teaching Awards Received: N/A 29. Teaching Specialization

2013 - Present Neurophysiology of the Visual System. Lecturer, 1 lecture/year Neuroscience and Behavioral Science Module - NBS 2013 – (M.D.

Students) University of Miami Miller School of Medicine, Miami, FL. 2013 – Present Neurophysiology of the Visual System Lecturer, 1 lecture/year Neuroscience and Behavioral Science Module - (M.D., M.P.H.

Students) University of Miami Miller School of Medicine, Miami, FL. 2013 – Present Naturally Occurring Neuronal Death. Lecturer, 1 lecture/year Developmental Neuroscience - NEU 663 – (Ph.D. Students) University of Miami Miller School of Medicine, Miami, FL. 2012 - 2014 Neuroinflammation Lecturer, 1 lecture/year Systems Neuroscience II – NEU 662 - (Ph.D. Students). University of Miami Miller School of Medicine, Miami, FL. 2012 Small groups Cardiovascular Physiology Module. (M.D. students) University of Miami Miller School of Medicine, Miami, FL. 2010 Small groups Cell Physiology and Membrane Biophysics. (M.D. students) University of Miami Miller School of Medicine, Miami, FL.

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2005 Small groups Cell Physiology and Membrane Biophysics. (M.D. students) University of Miami Miller School of Medicine, Miami, FL. 2001 Contact lenses fitting lab. (Opticianry students) 1 lecture/week in one semester Miami Dade College.

30. Thesis and Dissertation Advising/Post-doctoral student supervision:

2016 Scholarship Oversight Committee for fellow Fredrick Dapaah, MD. Department of Neonatology Miller School of Medicine, External Faculty member.

2011 - 2012 Christina Sedaghat (undergraduate): Honor’s Thesis: RIG-I

Signaling in the Pathogenesis of Alzheimer's Disease. University of Miami. Publication: de Rivero Vaccari JP, Brand FJ, Sedaghat C, W. Dalton Dietrich, Robert W. Keane. RIG-I Receptor Expression in the Pathology of Alzheimer’s Disease. J. Neuroinflammation. 2014 Apr 2;11(1):67.

VII. SERVICE 31. University Committee and Administrative Responsibilities

2016 – Present UMMSM Deans Diversity and Inclusion Council Committee, member

2011 – Present UMMSM Medical School Admissions Committee, member 2011 - 2016 Eastern Atlantic Student Research Forum, judge 2014 Eastern Atlantic Student Research Forum, paper judge 2013 Miami Project Summer Student Poster session, judge 2012, 2014 Miami Project Summer Student Fellows Admissions Committee,

member 2008 – 2012 Research & Creativity Forum, judge 2011 Students Training in Research (STIR) Program (High School

Student Marica Daniels), mentor 2009 16th Annual Florida-Georgia Louis Stokes Alliance for Minority

Participation (FGLSAMP) Expo, judge 2005 – 2007 Graduate Student Association, senator

32. Community Activities 2012 - Present Miami Art Museum/Perez Art Museum Miami, Charter Member. 2011 – Present History Miami Museum. Member.

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2007 – Present Institute of Budo Studies – Miami, Member. Ryushin Shouchi Ryu Kobudo (Yondan) Seitei Iaido (Shodan) Muso Jikiden Eishin Ryu Iaido (Shodan)

2000 – Present Florida Grand Opera, Patron. 2007 – 2015 Supernumerary for Florida Grand Opera

2015 Il Barbiere di Siviglia (Rossini) Directed by Dennis Garnhum 2015 Cosi Fan Tutte (Mozart) Directed by Bernard Uzan 2014 Tosca (Puccini) Directed by José María Condemi 2014 Nabucco (Verdi) Directed by Leigh Holman 2013 La Sonnambula (Bellini) Directed by Renata Scotto 2012 La Rondine (Puccini) Directed by Nicola Bowie 2011 Don Giovanni (Mozart) Directed by John Pascoe

2011 The Tales of Hoffman (Offenbach) Directed by Renaud Doucet

2010 Turandot (Puccini) Directed by Bliss Hebert 2010 Carmen (Bizet) Directed by Renaud Doucet 2010 Renaud Doucet 2009 Le Nozze di Figaro (Mozart). Directed by Stephen Lawless 2009 La Cenerentola (Rossini). Directed by A. Scott Parry 2008 La Traviata (Verdi). Directed by Bliss Hebert 2008 Giulio Cesare in Egitto (Handel). Directed by Mark Lamos 2008 Tosca (Puccini). Directed by Catherine Malfitano 2007 Cosi Fan Tutte (Mozart). Directed by David Gately

2011 Seitei Iaido demonstration at Japan Earthquake relief charity event. Japan Culture Day Program. Florida International University. April 30th, 2011.

2010 Jigen Ryu Iaido demonstration at the Japan Karate-Do International Tournament – Special 40th Anniversary Sensei Yasuyoshi Saito. Miami-Dade College Kendall Campus. March 12th, 2010.

2010 Brain Awareness Day 2010. Miami Museum of Science and University of Miami, volunteer.

2006 Crescendo Circle. Florida Grand Opera, member 2002 Vision screenings for the athletes of the Special Olympics through

the Special Olympics: Opening Eyes – Lions Club International Program. Tampa, FL

2001 Vision screenings at the First Annual Kids Wellness Day at Miami Dade College. Miami, FL

2001 Vision screenings at the Miami Book Fair International. Miami, FL.