JOURNAL OF BACTERIOLOGYVOLUME 145 * NUMBER 3 0 MARCH 1981

EDITORIAL BOARD

Stanley C. Holt, Editor (1982)Universitv of Massachusetts, Amherst

Samuel Kaplan, Editor (1983)University of Illinois, Urbana

June J. Lascelles, Editor (1984)University of California, Los Angeles

Mark Achtman (1982)James Akagi (1982)David Apirion (1982)Arthur 1. Aronson (1982)Gad Avigad (1983)Stephen D. Barbour (1982)Manfred E. Bayer (1982)Claire M. Berg (1983)Robert W. Bernlohr (1982)Terry J. Beveridge (1982)Dale C. Birdsell (1981)Edwin Boatman (1983)Winfried Boos (1982)H. D. Braymer (1982)Jean Brenchley (1983)Patrick J. Brennan (1981)Robert P. Burchard (1982)1. D. J. Burdett (1982)Richard 0. Burns (1983)Ercole Canale-Parola (1982)A. M. Chakrabarty (1983)Murray S. Cohn (1982)John W. Costerton (1983)Anthony R. Crofts (1982)John E. Cronan, Jr. (1983)Lolita Daneo-Moore (1981)Daniel V. Der Vartanian (1982)Walter J. Dobrogosz (1982)W. D. Donachie (1982)W. Ford Doolittle (1982)J. Allan Downie (1982)Gerhart Drews (1982)Charles Earhart (1981)A. Eisenstark (1982)David P. Fan (1981)Walton L. Fangman (1981)John D. Foulds (1982)

Simon Silver, Editor-in-Chief (1982)Washington Universitv, St. Louis, Mo.

Elizabeth McFall, Editor (1985)New York University, New York, N. Y.

Donald P. Nierlich, Editor (1982)University of California, Los Angeles

Allen T. Phillips, Editor (1985)Pennsylvania State University,

University Park

Ann Ganesan (1982)J. F. Gardner (1981)Robert Gennis (1982)Bijan K. Ghosh (1981)David T. Gibson (1981)Harry E. Gilleland, Jr. (1982)Patricia L. Grilione (1982)Walter R. Guild (1981)Tadayo Hashimoto (1982)Gerald L. Hazelbauer (1981)Charles E. Helmstetter (1982)Ulf Henning (1982)Peter Hirsch (1982)Bruce Holloway (1982)Philip Hylemon (1982)Karin Iher (1981)Joseph Inselburg (1981)Robert J. Kadner (1982)Eva R. Kashket (1981)Donald L. Keister (1983)Edward Kellenberger (1982)David E. Kennell (1983)Jordan Konisky (1981)K. W. Knox (1982)Tbomas G. Lessie (1982)Donald R. Lueking (1981)Ben J. J. Lugtenberg (1983)Carolyn MacGregor (1981)Manley Mandel (1981)Barry Marrs (1981)Ann Matthysse (1982)Neil H. Mendelson (1981)Sally Ann Meyer (1981)Robert V. Miller (1981)David Mount (1982)R. G. E. Murray (1983)Walter G. Niehaus, Jr. (1981)Hiroshi Nikaido (1981)

Robert Rownd, Editor (1985)Northwestern Medical School

Chicago, 111.Paul S. Sypherd, Editor (1984)University of California, Irvine

John H. Nordin (1982)Sunil Palkhaudhuri (1982)Leo Parks (1982)Martin Pato (1981)Olga Pierucci (1981)Patrick J. Piggot (1981)William S. Reznikoff (1982)Palmer Rogers (1981)Burton Rosan (1981)Barry P. Rosen (1983)Harry Rosenberg (1982)Antoinette Ryter (1982)Abigail Salyers (1981)Gene A. Scarborough (1982)June Scott (1981)Jane K. Setlow (1981)Peter Setlow (1981)J. A. Shapiro (1982)Lucille Shapiro (1983)Douglas W. Smith (1983)Issar Smith (1981)Paul F. Smith (1982)Dieter G. Soil (1982)A. L. Sonenshein (1983)Brian G. Spratt (1982)Eric J. Stanbridge (1981)Roger Storck (1981)Stanley Streicher (1983)1. W. Sutherland (1982)John L. Swanson (1981)Robert L. Switzer (1982)F. Robert Tabita (1981)Kenneth N. Tinuis (1981)Alexander Tomasz (1983)James L. Van Etten (1982)James A. Wechsler (1981)Henry C. Wu (1981)Howard Zalkin (1982)

Walter (G. Peter Ill, Managing EditorGisella Pollock. As.sistant Managing Editor Linda M. Illig, Production Editor

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EX OFFICIOAlbert Balows, President (1980-1981) Frederick C. Neidhardi, President-Elect ( 1980-1981 )

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Author IndexAdler, Julius, 1196Agabian, Nina, 1463Alver, Stein, 1413Armstrong, G. D., 1167Atkins, Daniel E., 1374

Baddiley, James, 1222Ball, Peter, 1417Bascomb, Newell F., 1266Benedict, Charles D., 1273Bhattacharya, Sudha, 1442Bird, Robert E., 1305Bittan, Raya, 1432Boye, Erik, 1413Bramucci, Michael G., 1281Brandriss, Marjorie C., 1359Bremer, Hans, 1232, 1239Buchanan, Christine E., 1293

Cain, Brian D., 1154Chan, R., 1386Chan, T. T. K., 1150Chapman, Astrid G., 1374Chiaverotti, Teresa A., 1463Chopra, Ian, 1417Choung, Kyung-Koo, 1239Churchward, Gordon, 1232Cooper, Ronald A., 1425Costerton, J. W., 1386, 1397Cox, John C., 1317

Deal, Carolyn D., 1154DeMoss, John A., 1317Docherty, Andrew, 1417Donnelly, Mark I., 1425Drews, Gerhart, 1121

Eccles, Sarah, 1417Edlind, Thomas D., 1436Edwards, Ellen S., 1317Emanuel, Janet R., 1342Estiva, Evangeline, 1232, 1239

Finck, Annette, 1144Fitzgerald, John W., 1428Fraley, Robert T., 1154Friedrich, Barbel, 1144Friedrich, Cornelius G., 1144

Frost, L. S., 1167

Gallant, Jonathan, 1463Garcia, Augusto F., 1121George, James R., 1428Glembotaki, Christoper C., 1374Gomez, Reinaldo F., 1456Grasmuk, Hans, 1273

Hall, Barry G., 1459Hancock, Ian C., 1222Heine, Elke, 1144Henry, Susan, 1404Hollinger, M. Flynn, 1448Horn, Virginia, 1334

Ihler, Garret M., 1436Imai, Ko, 1459Inouye, Masayori, 1257Inouye, Sachiye, 1137Irvin, R. T., 1386, 1397

Jacobson, Eric, 1452Jinks-Robertson, Sue, 1445

Kado, C. I., 1365Kaplan, Samuel, 1154Kihara, May, 1209Kinney, Dennis M., 1281Kooistra, Jan, 1189Kwon-Chung, Kyung J., 1410

Langer, Pamela J., 1310Lazzaroni, Jean-Claude, 1351Levitz, Ruth, 1432Linker, Carey, 1299Liu, S.-T., 1365

MacAlister, T. J., 1386, 1397Macnab, Robert M., 1209Magasanik, Boris, 1359Magee, P. T., 1342Mansour, James D., 1404McArthur, Hamish A. I., 1222McLean-Bowen, Colleen A., 1325Medoff, Gerald, 1452Medoff, Judith, 1452Mulfinger, Lorraine M., 1286

Nakazawa, Atsushi, 1137Nakazawa, Teruko, 1137Newman, E. B., 1150Nomura, Masayasu, 1445Noteborn, Matthieu, 1189Nuti, Marco P., 1129

Paranchych, W., 1167Parker, Gary, 1463Parks, Leo W., 1325Pellon, Jose R., 1456Phillips, Allen T., 1286Popkin, Terry J., 1410Portalier, Raymond C., 1351Prakash, Rameswaram K., 1129

Ray, Paul H., 1273Reidl, Horst H., 1121Repaske, David R., 1196Rhodes, Judith C., 1410Rottem, Shlomo, 1299

Sarkar, Nilima, 1442Schilperoort, Rob A., 1129Schmidt, Robert R., 1266Shales, Stuart, 1417Shanabruch, William G., 1310Shapiro, Lucille, 1404Skarstad, Kirsten, 1413

Tindol, Michael B., 1448Toh-e, Akio, 1421Torriani, Annamaria, 1249Turner, Katherine J., 1266

van Randen, Jan, 1177Venema, Gerard, 1177, 1189Vogel, H. J., 1167

Walker, Graham C., 1310Wickner, Reed B., 1421Wilson, T. Hastings, 1299Wood, David O., 1448

Yagil, Ezra, 1432Yanofsky, Charles, 1334Yee, Thomas, 1257Yeung, Anthony T., 1266

Zuckier, Gerald, 1249

AUTHOR INDEX

VOLUME 145

Achtman, Mark, 840Adachi, Osao, 814Adler, Julius, 1196Agabian, Nina, 1463Ahrne, S., 74Akhmedov, A. T., 21Albritton, William L., 1099Alver, Stein, 1413Amelunxen, Remi E., 503Ameyama, Minoru, 814Andrews, Robert E., Jr., 1052Arai, Mamoru, 657Arai, Takao, 803Archibald, Frederick S., 442Armstrong, G. D., 1167Aronson, Arthur I., 541Asada, Makoto, 559Ash, Greg, 200Atkins, Daniel E., 1374

Baddiley, James, 1222Ball, Peter, 1417Barbour, Stephen D., 914Baron, L. S., 1010Bascomb, Newell F., 1266Bicalupo, M., 1079Beachey, Edwin H., 620Becker, Michael, 200Beckwith, Jon, 288, 293Begueret, Joel, 82Bekker, M. L., 21Belaich, Jean-Pierre, 191Bendler, John W., 1099Benedict, Charles D., 1273Bentley, Ronald, 328Berg, Claire M., 984Beutin, Lothar, 840Bhattacharya, Sudha, 1442Bieth, Gilda, 1102Bird, Robert E., 1305Biswas, Gour D., 638Bittan, Raya, 1432Blumenberg, Miroslav, 664Boehmer, Sieghild, 306Botta, Giuseppe, 632Botta, Giuseppe A., 333Bougueleret, Lydie, 1102Bowles, L. K., 668Boye, Erik, 1091, 1413Bradley, Doug, 704Bramucci, Michael G., 1281Brandriss, Marjorie C., 1359Braun, Volkmar, 156Bremer, Hans, 1232, 1239Brill, Winston J., 248, 348, 743,

1116Brown, Moira G. M., 780

Buchanan, Christine E., 1293Bulawa, Christine E., 113Bulla, Lee A., Jr., 1052Burris, Robert H., 824Byng, Graham S., 752

Cain, Brian D., 1154Callister, Heather, 1042Carey, Alice M., 628Carrasco, Alicia, 164Carreon, Roberto R., 272Carty, C. E., 472Chan, R., 1386Chan, T. T. K., 1150Chang, Shing, 422Chapman, Astrid G., 1374Charnetzky, W. T., 452Chen, Chai-Pao, 926Chen, Min, 466Chiaverotti, Teresa A., 1463Chopra, Ian, 1417Choung, Kyung-Koo, 1239Churchward, Gordon, 1232Clark, Alvin J., 521Clewell, Don B., 494Cocito, C., 775Collins, Hugh H., 1010Cooper, Ronald A., 1425Costerton, J. W., 1386, 1397Costerton, J. William, 628Cox, Graeme B., 200Cox, John C., 1317Crabb, John W., 503Csonka, esz16 N., 299Curtis, Susan J., 398

Davidson, Loren I., 1052Davies, John K., 788Davis, Rowland H., 14Deal, Carolyn D., 1154Dean, Donald H., 980de Boer, Wim R., 50De Jong, M. Henk, 27Dellers, Elizabeth A., 321DeMoss, John A., 1317Dennis, Patrick, 641Deshpande, Kathryn L., 768DesRosier, John P., 613DeVoe, I. W., 280Docherty, A., 129Docherty, Andrew, 1417Don, R. H., 681Donnelly, Mark I., 1425Dougan, Gordon, 920Dow, J. Maxwell, 272Downie, J. Allan, 200Doyle, Ronald J., 878

Drews, Gerhart, 1121Dubnau, D., 129Duncan, Bruce K., 306, 687Dunn-Coleman, Nigel S., 884Dyess, Barbara T., 429

Eccles, Sarah, 1417Edlind, Thomas D., 1436Edwards, Ellen S., 1317Eisenstein, Barry I., 620Emanuel, Janet R., 1342Engstrom, Peter, 35, 43Estiva, Evangeline, 1232, 1239

Falkow, Stanley, 920Federici, G., 1079Fehr, Susanne, 68Feige, U., 138Finck, Annette, 1144Fitzgerald, John W., 1428Fleckenstein, Jim, 429Flores, Enrique, 175Foley, Diana, 641Foster, John W., 1002Fouts, Ken E., 914Fraley, Robert T., 1154Franke, Arthur E., 494Freese, Ernst, 760Fridovich, Irwin, 442Friedrich, Barbel, 1144Friedrich, Cornelius G., 1144Frost, L. S., 1167Fujita, Yasutaro, 760Fukuda, Akio, 559Fukunaga, Yukiko, 808

Gallant, Jonathan, 641, 1463Gallori, E., 1079Garcia, Augusto F., 1121Garrett, Charles, 687Garrett, Reginald H., 884Georgalis, Yannis, 513George, James R., 1428Gherna, Robert L., 752Ghuysen, J. M., 775Gibson, Frank, 200Gillen, Jane R., 521Gilpin, Richard W., 651Glembotski, Christoper C., 1374Goebel, W., 369, 375Goebel, Werner, 233Gomez, Reinaldo F., 1456Gotz, F., 74Grandi, G., 129Grandi, R., 129Grasmuk, Hans, 1273

ii AUTHOR INDEX

Gray, Oanh, 422Gromkova, Rosa, 1075Gryczan, T. J., 129Guerrero, Miguel G., 175Guinand, Micheline, 675Gunge, Norio, 382Gurlitz, Robin H. G., 583Guzman-Verduzco, Luz Maria,

722

Hadden, Charles T., 434Hagblom, Per, 788Hageman, James H., 489Hall, Barry G., 1459Hamilton, James W., 503Hancock, Ian C., 1222Hancock, Robert E. W., 628Harada, Tokuya, 358, 796Harayama, Shigeaki, 43Harkness, Robin E., 845Harris, Charles L., 1031Hatchikian, Claude E., 191Hayashi, Shigeru, 657Hays, John B., 306Hazelbauer, Gerald L., 35, 43Heasley, Franklin A., 624Heckels, John E., 736Heine, Elke, 1144Henry, Susan, 1404Herman, Gail E., 644Herrero, Antonia, 175Hill, John E., 696Hillier, Sharon, 452Hirota, Yukinori, 657Hollinger, M. Flynn, 1448Holloway, B. W., 145Holmes, Kathryn V., 583Horn, Virginia, 1334Horodniceanu, Thea, 1102Horwitz, Arnold H., 873Hotani, Hirokazu, 1082Howe, Martha M., 299Hulett, F. Marion, 926, 934Humphreys, G. O., 780Hutten, Theo J., 27

Ihler, Garret M., 1436Imai, Ko, 1459Ingraham, John L., 299Ingram, L. O., 472Inoue, Masakazu, 729Inouye, Masayori, 417, 654, 1257Inouye, S., 221Inouye, Sachiye, 1137Inukai, Masatoshi, 657Inukai, Masatoski, 654Irikura, Maki, 953Irvin, R. T., 1386, 1397Irvin, Randall T., 628Ishiguro, Edward E., 845Ishihara, Akira, 1082Itoh, Yoshifumi, 122

Jacobson, Eric, 1452

Jaffor Ullah, Abul H., 958Janczura, E., 775Jensen, Roy A., 752Jinks-Robertson, Sue, 1445Johnson, E. M., 1010Jones, David T., 1

Kaboev, 0. K., 21Kado, C. I., 1365Kaguni, Jon M., 974Kaguni, Laurie Simon, 974Kahn, Marc E., 1075Kamio, Yoshiyuki, 122Kane, James F., 768Kaplan, Samuel, 1154Karska-Wysocki, Barbara, 573Katze, Jon R., 768Kelley, Jenny T., 1018Kelln, Rod A., 1095Kihara, May, 1209Kinney, Dennis M., 1281Klevickis, Stephen, 248Knight, Ralph A., 651Komeda, Yoshibumi, 1036Konisky, Jordan, 341, 647, 668Kooistra, Jan, 1189Kopecko, Dennis J., 1010Koyasu, Shigeo, 559Kramer, Karl J., 1052Kruyssen, Fred J., 50Kupersztoch-Portnoy, Yankel

M., 722Kurelec, Branko, 548Kurogi, Yumiko, 953Kusano, Tomonobu, 122Kwon-Chung, Kyung J., 1410

Lai, Jiunu-Shyong, 657Langer, Pamela J., 1310Langman, Lyndall, 200Lara, J. Cano, 613Laszlo, Daniel J., 990Lazzaroni, Jean-Claude, 1351Le Gall, Jean, 191Leonard, John E., 1106Levitz, Ruth, 1432Leyh-Bouille, M., 775Lindahl, Lasse, 459Lindberg, M., 74Linker, Carey, 1299Liu, Chi-Li, 966Liu, S.-T., 1365Loeb, Marilyn R., 596Lohr, John R., 980Lombardo, Linda Rivoire, 321Luchkina, L. A., 21Lucu, Cedomil, 548Lysko, Paul G., 946

Maas, Renata, 97Maas, Werner K., 97MacAlister, T. J., 1386, 1397Macnab, Robert M., 1209

J. BACTERIOL.

MacNeil, Douglas, 348Macrina, Francis L., 867Magasanik, Boris, 257, 266, 664,

1359Magee, P. T., 1342Magee, Paul T., 896Maier, Robert J., 533Maloy, Stanley R., 1110Mamet-Bratley, Margaret D.,

573Manning, Paul A., 840Mansour, James D., 1404Martin, Phyllis A. W., 980Martin, Ronald R., 713Martinez-Cadena, MariaGuadalupe, 722

Mata-Gilsinger, Mireille, 181Matsumoto, Akira, 605Matsumoto, H., 145Matsushita, Kazunobu, 814Matthysse, Ann G., 583Mayer, H., 138Mazaitis, Anthony J., 97McArthur, Hamish A. I., 1222McCammon, Mark T., 106McLean-Bowen, Colleen A., 1325McLinden, James H., 503Medoff, Gerald, 1452Medoff, Judith, 1452Meganathan, R., 328Mernoff, David, 417Michaelis, Susan, 288, 293Michel, Georges, 675Mirelman, David, 845Mizushima, Shoji 1085Modrich, Paul, 644Morioka, Toshio, 729Morse, Stephen A., 946Mulfinger, Lorraine M., 1286Muller, Isabel, 548Muller, Werner E. G., 548Murdock, Archie L., 503Murooka, Yoshikatsu, 358, 796Mutoh, Norihiro, 1085

Nagasawa, Tadako, 1085Nakamura, Kenzo, 417, 654Nakayama, Tatsuo, 953Nakazawa, Atsushi, 1137Nakazawa, Teruko, 1137Neidhardt, Frederick C., 513Neuhard, Jan, 687Newman, E. B., 1150Nishijima, Masahiro, 113Noegel, Angelika, 233Nohara, Chieko, 808Nomura, Masayasu, 1445Nordin, Philip, 1052Norlander, Lena, 788Normark, Staffan, 788Noteborn, Matthieu, 1189Nunn, William D., 1110Nuti, Marco P., 1129

VOL. 145, 1981

O'Day, Kathy, 904Ogrydziak, David M., 404Okada, Yoshimi, 559Oliveira, Maria B. M., 171Ordal, George W., 958Oshima, Y., 221Ozawa, Fumiko, 382

Pao, Chia C., 429Paranchych, W., 1167Paris, C. Gregory, 257, 266Park, James T., 333, 632Park, James V., 704Parker, Charlotte D., 1018Parker, Gary, 1463Parks, L. W., 106Parks, Leo W., 1325Pattee, Peter A., 479Patterson, Suzanne K., 651Paulus, Thomas J., 14Peck, H. D., Jr., 966Peeters, Ben P. H., 27Pellon, Jose R., 1456Pemberton, J. M., 681Pepino, R., 1079Perkins, H. R., 780Perrot, Michel, 82Peters, Wendy, 904Pfeifer, F., 369, 375Philbrick, William M., 657Phillips, Allen T., 1286Phillips, Teresa A., 513Pienkos, Philip T., 248, 743Pierson, Leland S., III,299Pilacinski, W. P., 1025Pincheira, Guido, 164Polsineii, M., 1079Popkin, Terry J., 1410Portalier, Raymond, 211Portalier, Raymond C., 1351Prakash, Rameswaram K., 1129Primakoff, Paul, 410Projan, Steven J., 861

Radziejewska-Lebrecht, Joanna,138

Raetz, Christian R. H., 113Raina, Jawahar L., 661Raugei G., 1079Ray, Dan S., 974Ray, Paul H., 1273Rdest, Ursula, 233Reidl, Horst H., 1121Repaske, David R., 1196Reusch, R. N., 889Rhodes, Judith C., 1410Richter, Dietmar, 68Ritzenthaler, Paul, 181Robert-Baudouy, Janine, 211Rottem, Shlomo, 1299Royle, P. L., 145

Sadoff, H. L., 889

AUTHOR INDEX m

Saier, Milton H., Jr., 391, 1106Sakaguchi, Kenji, 382Salyers, Abigail A., 8Sarkar, Nilima, 1442Sarthy, Aparna, 288,293Sato, Takashi, 88Saunders, J. R., 780Schaus, Nancy, 904Schilperoort, Rob A., 1129Schmidt, E. L., 1025, 1063Schmidt, Laura S., 632Schmidt, Mary R., 391Schmidt, Robert R., 1266Sekar, Vaithilingam, 489Shales, Stuart, 1417Shanabruch, William G., 1310Shapiro, Lucille, 1404Shaw, Karen J., 984Shinagawa, Emiko, 814Shipley, Patricia L., 920Shivakumar, A. G., 129Shizuya, Hiroaki, 1113Simms, Paula C., 404Skarstad, Kirsten, 1413Smith, David H., 596Smith, Martin W., 513Smith, Richard D., 8Snellings, Norma J., 1010Soll, Larry, 704Solomon, Solomon S., 620Sparling, P. Frederick, 638Spencer, Donald B., 926, 934Steen, Harald B., 1091Stelow, Jane K., 1099Stieglitz, Heather, 722Stoeber, Francois, 181, 211Streips, Uldis N., 878Strominger, Jack L., 398Subramaian, Alap R., 513Suzuki, Takahito, 1036Suzuki, Tsuneo, 503Sweet, William J., 824

Taber, H. W., 328Taber, Harry W., 321Takehara, Tadamichi, 729Takizawa, Noboru, 358Tamaru, Atsuko, 382Tanaka, Michiyasu, 808Taylor, Barry L., 990Taylor, Kent D., 1113Terawaki, Yoshiro, 122te Riele, Hein P. J., 832Thorlton, Cydney L., 713Timko, Jozef, 873Tindol, Michael B., 1448Toh-e, Akio, 221, 1421Torriani, Annamaria, 1249Traore, Alfred S., 191Tsien, H. C., 1063Turnbough, Charles L., Jr., 299Turner, Katherine J., 1266Tyrell, Dana J., 1052

Uhlenbruck, Gerd, 548Unger, Leon, 713

Ureta, Tito, 164

Vacheron, Marie-Jeanne, 675VanBogelen, Ruth A., 513van der Drift, Chris, 27van Randen, Jan, 1177van Sluis, Paul, 832Venema, Gerard, 832, 1177, 1189Villa, Vicente D., 272Vogel, H. J., 1167Vogels, Godfried D., 27

Wagegg, Wilhelm, 156Wake, R. G., 1042Walker, Graham C., 1310Wannamaker, Lewis W., 696Warner, Huber R., 687Wechsler, James A., 861Weckesser, J., 138Weidinger, G., 369, 375Welch, Rodney A., 867Weston, A., 780Whelan, William L., 896Whitaker, Robert J., 752White, Terry B., 878Wickner, Reed B., 1421Wilcox, Gary, 873Willetts, Neil, 840Willis, David K., 521Wilson, Selma P., 489Wilson, T. Hastings, 1299Wolf, Marcia K., 341Wolin, M. J., 466Wood, David O., 1448Woods, David R., 1Worsham, Patricia L., 647Wouters, Jan T. M., 50Wright, Andrew, 904Wr6blewski, Henri, 61Wu, Henry C., 657

Yagil, Ezra, 1432Yaginuma, Katsuyuki, 559Yamagishi, Saburo, 808Yamaguchi, Shigeru, 1082Yamamoto, Tatsuo, 850Yamamoto, Tomoko, 808Yanofsky, Charles, 1334Yasumura, Mitsue, 417Yee, Thomas, 1257Yeung, Anthony T., 1266Yokogawa, Kanae, 729Yokota, Takeshi, 850Yoshida, Hideya, 559Yu, E. K. C., 280Yura, Takashi, 88

Zachary, Arthur L., 596Zahn, Rudolf K., 548Zak, Victor L., 1095Zancan, Glaci T., 171Zelinka, Jan, 873Zengel, Janice M., 459Zhu, Jiabi 348, 1116Zuckier, Gerald, 1249Zwiebel, Laurence J., 654

SUBJECT INDEX

VOLUME 145

Adenosine triphosphate complex, Fo portion ofE. coli, 200uncB, 200uncE, 200uncF, 200

S-Adenosylhomocysteine analogsS. cerevisiae, 106sterol transmethylation, 106

Adenylate energy chargeE. coli CR341T28, 1374

Adenylate kinaseE. coli CR341T28, 1374

AdhesionH. parainfluenzae, 1075

Aerotaxiselectron transport, 990S. typhimurium, 990

Agrobacterium spp.plasmid detection and isolation procedure, 1365

Agrobacterium tumefacienscellulose fibrils, 583

D-Alanrne carboxypeptidaseE. coli, 845peptidoglycan cross-linkage, 845

Alcaligenes eutrophushydrogenase formation, 1144nickel requirement, 1144pesticide degradation, 681plasmids, 681

Alcaligenes paradoxuspesticide degradation, 681plasmids, 681

Alkaline extracellular proteaseS. lipolytica, 404XPR2, 404

Alkaline phosphataseB. licheniformis, 926, 934cobalt, 926lactoperoxidase-'2I localization, 934

Allostery of 3-deoxy-D-arabino-heptulosonate 7-phos-phate synthetase

pseudomonad relatedness, 752Amber mutations

E. coli, 459temperature-sensitive suppression, 459

Amber suppressionE. coli, 704tRNAG'n Su+2 mutants, 704

y-A_minobutyrateE. coli grown on, 1425succinic semialdehyde dehydrogenases, 1425

2-Amino-5-ethoxycarbonylpyrimidine-4(3H)-oneB. subtilis growth, 1079

AminotransferaseK. aerogenes, 266

Ammonium ionsnitrogenase activity, 824

R. rubrum, 824Anabaena 8p. strain 7119

nitrate reductase, 175Anacystis nidulans

nitrate reductase, 175A-protein

E. coli, 513D-Arabinose dehydrogenaseN. crassa, 164

Arginine metabolismregulation by compartmentation, 1359S. cerevisiae, 1359

Aromatic amino acid aminotransferaseK. aerogenes, 266

Aromatic amino acid regulationK. aerogenes, 257tryptophan metabolism, 257

Arsenatechemotaxis, 803E. coli, 803

ArylsulfatasePseudomonas sp. strain C12B, 1428S. typhimurium, 796tyramine oxidase regulation of derepressed synthe-

sis, 796Ascosporogenesis

ribosome synthesis, 1342S. cerevisiae, 1342

Azotobacter vinelandiiencystment, 889lipid metabolism, 889molybdate, 248molybdenum accumulation and storage, 743nitrogenase activation, 248

Bacillus brevisDNA replication inhibition, 1442

Bacillus cereuspili from spores, 613spore coat protein, 541

Bacillus coagulansglyceraldehyde-3-phosphate dehydrogenase, 503

Bacillus licheniformisalkaline phosphatase, 926, 934cobalt, 926cytoplasmic membrane, 934penG cloning, 422

Bacillus sphaericusLL-oligopeptidase, 675sporulation, 675

Bacillus stearothermophilusDNA polymerase, 21guanosine 3',5'-polyphosphate synthetases, 68

Bac*lus subtilis2-amino-5-ethoxycarbonylpyrimidine-4(3H)-one,

1079cell wall turnover, 50

iv

VOL. 145, 1981

chemotactic methylation, 958dihydroxynaphthoate synthase, 328division initiation mutants, 1042DNA fragment assimilation, 1177fdpAl, 760fructose-bisphosphatase mutant, 760glutamine, 768growth, 1079inosine, 489L-form growth parameters in batch culture, 651macrolide-lincosamide-streptogramin B resistance,

129menaquinone biosynthesis, 321men gene clustering, 321men mutants, 321, 328methylation, chemotactic, 958nitrogen metabolism, 768nucleoids, 1177nucleoside, 489penG cloned in, 422phage PMD12, 1281polypeptide P6, 953postreplication repair, 434pyrimidine analog, 1079recombination, 1177sporogen, 489sporulation, 489, 1281o-succinylbenzoyl-coenzyme A synthetase, 328teichoic acid biosynthesis, 1222tetracycline accumulation, 1417tmsl2, 1042transformation of L-form, 878transforming DNA, 434transforming DNA, inactivation of, 8324,5',8-trimethylpsoralen monoadducts, 832tsl, 1042tsl2, 1042UV irradiation, 434

Bacillus thuringiensisparasporal crystals, 1052plasmid pC194, 980protoplast transformation, 980transformation of protoplasts, 980

Bacteriophage A25group A streptococci, 696lysin, 696

Bacteriophage lambdacarrying structural gene for a major outer membrane

matrix, 1085recombination of uracil-containing phages, 306

Bacteriophage A-P22 hybridrequirements for growth in E. coli, 1113

Bacteriophage M13 oriCreplication in E. coli rep mutant, 974

Bacteriophage Muhost range expansion, 358

Bacteriophage PMB12B. subtilis sporulation, 1281

Bacteriophages 425 and 029B. subtilis L-form transformation, 878

Bacteriophage T7 (alkylated)E. coli, 573

Bacteroides fragilisliquid holding recovery, 1

SUBJECT INDEX V

plasmid pBF4, 867Bacteroides species

fermentations, 466growth, 466heme, 466vitamin B12, 466

Bacteroides thetaiotamicronleucine incorporation, 8phospholipid, 8

B56.5 proteinE. coli, 513

Cadaverinepeptidoglycan, 122S. ruminantium, 122

CalciumDNA binding, 780E. coli transformation, 780

Candida albicansheterozygosity, natural, 896

Carbamyl aspartate5-fluorouracil resistance, 1095S. typhimurium, 1095ubiquinone biosynthesis, 1095

CarbohydrateColB2 pin, 1167EDP208 pilin, 1167

Carbon sourceE. coli, 1150threonine, 1150

Catabolite repressionE. coli heat-stable enterotoxin activity, 722

Caulobacter crescentusphospholipid biosynthesis, 1404polar morphogenesis, 559ppGpp accumulation, 1463stalk elongation, 1404

Cell envelopeN. gonorrhoeae, 946oxygen uptake, 946steroid hormones, 946

Cellulose fibrilsA. tumefaciens, 583

Cell volume regulationM. gallisepticum, 1299proton motive force across membrane, 1299

Cell wall synthesisM. rouxii, mycelial, 272

Cell wall turnoverB. subtilis, 50

Chemoautotrophic growth with H2R. japonicum mutants, 533

Chemotactic methylationB. subtilis, 958

Chemotaxisarsenate, 803cytoplasmic pH, 1209E. coli, 803, 1196, 1209intracellular pH, 1196Salmonella, 1209

Chemotaxis protein III, methyl-acceptingtrg, 43

Chemotaxis proteins, methyl-acceptingE. coli, 35, 43

vi SUBJECT INDEX

III, 43multiple forms, 35

Chiamydia psittaciintracytoplasmic inclusions containing, 605

Chloramphenicolmembrane proteins, 417P. aeruginosa, 417

Chlorella sorokinianaammonium-inducible glutamate dehydrogenase,

1266Chromosome repairE. coli, 1456thermal damage, 1456

Chromosome replicationE. coli, 1232growth rate-dependent control of initiation, 1232

CircularityP. aeruginosa chromosome, 145

cir regulationE. coli, 647

Citrate-tris(hydroxymethyl)aminomethaneE. coli 08, 1386outer membrane release, 1386

Citrobacter freundiiVi antigen expression, 1010

CloningB. licheniformispenG gene, 422TOL genes xylB and xylE in E. coli, 1137

Cloning vectorP. aeruginosa, 1448

CobaltaLkaline phosphatase, 926B. licheniformis, 926

Coenzyme M derivativesmethane formation, 27

ColB2 pilncarbohydrate, 1167phosphate, 1167

Colicin Iacleavage, 668E. coli, 668

Colicin Ia receptorE. coli, 647transcriptional regulation, 647

Coliphage Mu dl (Apr lac)pyr.:lac fusions, 299S. typhimurium, 299

Complementation testsphoS andphoT mutants of E. coli, 1432

Corynebacteria, leprosy-derivedpeptidoglycan, 775

Cryptococcus laurentiiseptal complex in hyphae, 1410

Cyanobacterianitrate reductase, 175

Cyclic adenosine 3',5'-monophosphateE. coli, 620fimbriae, type 1, 620H. capsulatum dimorphism, 1452

Cyclic adenosine 3',5'-monophosphate levelP. aeruginosa, 1286P. putida, 1286

CysteineE. coli, 1031

J. BACTERIOL.

growth inhibition, 1031threonine deaminase, 1031

L-Cysteine oxidaseN. meningitidis membrane, 280

Cytidine 5'-triphosphate:cytidine 5'-monophosphate-3-deoxy-D-manno-octulosonate cytidylyltransfer-ase

E. coli, 1273Cytoplasmic membrane

alkaline phosphatase, 934B. licheniformis, 934

dam methylaseE. coli, 644

3-Deoxy-D-arabino-heptulosonate 7-phosphate syn-thetase

pseudomonad relatedness, 752Deoxyribonucleic acid

B. subtilis, 832calcium-induced binding, 780E. coli, 687E. coli tox genes, 850E. coli transfonnation, 780entry of double-stranded DNA during transforma-

tion, 638fragment assimilation by B. subtilis nucleoids, 1177inactivation of transforming DNA by 4,5',8-tri-

methylpsoralen monoadducts, 832intrastrand base pairing, 1436modifications in N. gonorrhoeae, 788N. gonorrhoeae, 638plasmid pCR1, 1436uracil containing, 687

Deoxyribonucleic acid entryEDTA, 1189H. influenzae, 1189

Deoxyribonucleic acid methylaseE. coli, 644

Deoxyribonucleic acid polymeraseB. stearothermophilus, 21

Deoxyribonucleic acid replicationB. brevis, 1442E. coli, permeable and viable cells, 1413E. coli mutant, 1239inhibition by RNA polymerase inhibitors, 1442

Desulfotomaculum spp.pyrophosphate, 966sulfate reduction, 966

Desulfovibrio spp.pyrophosphate, 966sulfate reduction, 966

Desulfovibrio vulgarisgrowth energetics, 191

Dihydroxynaphthoate synthaseB. subtilis men mutants, 328

DimorphismcAMP regulation, 1452H. capsulatum, 1452

Division initiation mutantsB. subtilis, 1042

dnaA(Am)E. coli, 904

dnaA suppressorsE. coli, 861

SUBJECT INDEX vii

EDP208carbohydrate, 1167phosphate, 1167

Electron transportaerotaxis, 990S. typhimurium, 990

Elongation factor GE. coli, 429ppGpp synthesis, 429

eltE. coli, 97plasmid pCG86, 97

EncystmentA. vinelandii, 889lipid metabolism, 889

Endo-a-1,3-glucanaseS. chartreusis, 729

EndonucleaseS. aureofaciens, 873

Enterotoxin activity, heat-stablecatabolite repression, 722E. coli, 722

Enterotoxin genesDNA sequences, repeated, 850E. coli, 850tox, 850

Enterotoxin productionE. coli, 97elt, 97estA, 97plasmid pCG86, 97

Enzymes IIE. coli, 391transphosphorylation, 391

Erwinia spp.plasmid detection and isolation procedure, 1365

Escherichia coliadenosine triphosphate complex, Fo portion, 200adenylate kinase from strain CR341T28, 1374D-alanine carboxypeptidase and peptidoglycan

cross-linkage, 845amber mutations, 459amber suppression, 704y-aminobutyrate, growth on, 1425A-protein, 513arsenate, 803B56.5 protein, 513cAMP, 620carbon source, 1150chemotaxis, 803, 1196, 1209chemotaxis proteins, methyl-accepting, 35, 43chromosome repair, 1456chromosome replication initiation, 1232cir regulation, 647citrate-Tris-mediated release of outer membrane

sections, 1386cloning of TOL genes xylB and xylE, 1137CMP-KDO synthetase, 1273colicin la cleavage, 668colicin Ia receptor, 647, 668complementation tests betweenphoS andphoTmu-

tants, 1432cysteine, 1031cytoplasmic pH, 1209

dam methylase, 644DNA, 687dnaA(Am), 904DNA binding, 780DNA methylase, 644DNA replication, 1239DNA replication in permeable and viable cells, 1413DNA sequences, repeated, 850dna suppressors, 861dual-parameter flow cytophotometry, 1091elongation factor G, 429elt, 97enterotoxin activity, heat-stable, 722enterotoxin genes, 850enterotoxin production, 97enzymes II, 391estA, 97exu region, 181exuR gene product, 211FecA protein, 156ferric citrate transport, 156fimbriae, type 1, 620flagellar structures, incomplete, 1036fla mutants, 1036formate dehydrogenases, selenium-containing, 1317furazlocillin, 632,B-galactosidase synthesis, 641globomycin, 657groE gene product, 513growth, 1091growth inhibition, 1031heat-labile enterotoxin genes, 850hexuronate system genes, 181, 211hydrophobic, photolabile probe binding, 341intracellular pH, 1196K88ac adherence antigen, 920kasugamycin resistance gene, 914ksgB, 914ksgD, 914lipid synthesis, 472Ipp deletion mutations, 654membrane, outer, 1386, 1397membrane potential, 341mercury resistance, 97methyl-accepting chemotaxis proteins, 35, 43mismatch correction, 644multicopy extragenic suppressors of dna mutations,

861murein lipoprotein, 657murein synthesis, 333nucleoid repair, 1456OmpC, 88OmpF, 88OmpF protein, 1085oriC-dependent duplex DNA replication, 974outer membrane permeability, 1397outer membrane protein FecA, 156outer membrane proteins, 88outer membrane release,-1386oxa gene transposition, 808penG cloned in, 422penicillin-binding protein 3, 333penicillin-binding protein 2, 398penicillin-binding proteins, 632, 1293

VOL. 145, 1981

viii SUBJECT INDEX

peptide chain elongation factor G, 429peptidoglycan cross-linkage, 845periplasmic-leaky mutants, 1351pgsA, 113pgsB, 113pH, intracellular, 1196phage lambda carrying the structural gene for amajor outer membrane matrix protein, 1085

phage M13 oriC replication, 974phage A-P22 hybrid, 1113phage T7 (alkylated) infection, 573phoA, 288, 293phoS24(Am), 1249phoS and phoT mutants, 1432phosphate-specific transport mutants, 1249phosphatidylglycerol synthesis, 113phoT35, 1249plasmid CoIEl, homology with p15A, 1305plasmid p15A, homology with CoIEl, 1305plasmid pCG86, 97plasmid pHlyl52, 233plasmid RP1, 713ppGpp synthesis, 429protein A, 513protein B56.5, 513pst-2, 1249RecE pathway of genetic recombination, 521rel defect, 641ribosomal protein synthesis in mutant, 1445rifampin resistance mutations, 1334RPl-Hfr strain formation, 713rpoB mutations, 1334septal murein synthesis, 333sfrA and sfrB products, 840streptomycin resistance, 97succinic semialdehyde dehydrogenases, 1425sulfonamide resistance, 97suppressor efficiency, 704temperature-sensitive suppression of amber muta-

tions, 459tetracycline accumulation, 1417tetracycline resistance, 97, 1110thermal damage to nucleoid, 1456threonine as carbon source, 1150threonine deaminase, 1031TOL genes xylB and xylE, 1137tox, 850transcriptional control factors, 840transcription termination, 1334transformation, 780transphosphorylation, 391trg, 43Tris modification of membrane permeability, 1397tRNAGI, Su 2 mutants, 704tryptophan operon attenuator, 1334uncB, 200uncE, 200uncF, 200uracil-containing DNA, 687uxu operon, 211uxuR gene product, 211xylB, 1137xylE, 1137

Escherichia spp.

plasrnid detection and isolation procedure, 1365estA

E. coli, 97plasmid pCG86, 97

Ethylenediaminetetraacetic acidDNA entry, 1189H. influenzae transformation, 1189recombination, 1189

exu genescloning, 181E. coli, 181

exuR gene productE. coli, 211uxu operon regulation, 211

fdpAIB. subtilis, 760fructose-bisphosphatase, 760

FecA proteinE. coli, 156ferric citrate transport, 156

Ferric citrate transportE. coli, 156outer membrane protein FecA, 156

Fimbriae, type 1cAMP, 620E. coli, 620

Flagellapassive rotation on paralyzed S. typhimurium (mot)

mutants, 1082Flagellar structures, incompleteE. coli mutants, 1036

fla mutantsE. coli, 1036

5-Fluorouracil resistancecarbamyl aspartate, 1095S. typhimurium, 1095ubiquinone biosynthesis, 1095

Formate dehydrogenasesE. coli, 1317resolution, 1317selenium containing, 1317

Fructose-bisphosphataseB. subtilis mutant, 760fdpAl, 760

D-Fructose dehydrogenaseG. industrius, 814

FurazlocillinE. coli, 632penicillin-binding protein, 632

,f-Galactosidase synthesisE. coli 641rel defect, 641

Genome sizeM. xanthus, 1257quantitation of individual restriction fragments,

1257renaturation analysis, 1257S. aurantiaca, 1257

gin-lb mutationN. crassa, 884nitrogen metabolite repression, 884

Globomycin

J. BACTERIOL.

VOL. 145, 1981

E. coli, 654, 657lpp deletion mutations, 654murein lipoprotein, 657S. typhimurium, 657

a-Glucan degradationglucose, 171P. circinatus, 171

Gluconobacter industriusD-fructose dehydrogenase, 814

Glucosea-glucan degradation, 171P. circinatus, 171

Glutamate dehydrogenase, ammonium-inducibleC. sorokiniana, 1266inactivation, 1266turnover during induction, 1266

GlutamineB. subtilis, 768nitrogen metabolism enzymes, 768

Glyceraldehyde-3-phosphate dehydrogenase, thermo-philic

B. coagulans, 503sequence homology in amino-terminal and active-

site regions, 503Glyoxylate bypass enzymes

Yersinia species, 452groE gene product

E. coli, 513Group A streptococcal bacteriophage lysin, 696Growth

2-amino-5-ethoxycarbonylpyrimidine-4(3H)-one,1079

B. subtilis, 1079cysteine, 1031dual-parameter flow cytophotometry, 1091E. coli, 1031, 1091threonine deaminase, 1031

Growth energeticsD. vulgaris, 191

Guanosine 5'-diphosphate 3'-diphosphateE. coli, 429elongation factor G, 429

Guanosine 3',5'-polyphosphate synthetasesB. stearothermophilus, 68

Guanosine tetraphosphate accumulationC. crescentus, 1463

Haemophilus influenzaeDNA entry, 1189EDTA, 1189membrane of encapsulated strain, 596recombination, 1189transformation, 1099, 1189

Haemophilus parainfluenzaeadhesion, 1075pili, 1075

Halichondria panicealectin, 548symbiosis with P. insolita, 548

Halobacteriagenetic variability, 375plasmids, 369

Heat-labile enterotoxin genesDNA sequences, repeated, 850

SUBJECT INDEX ix

E. coli, 850HemeBacteroides species, 466

Heptose regionlipopolysaccharide, 138R. tenue, 138

HeterozygosityC. albicans, 896

Hexuronate system genesE. coli, 181, 211exu region, 181exuR gene product, 211uxu operon, 211uxuR gene product, 211

Histidase levelP. aeruginosa, 1286P. putida, 1286

Histoplasma capsulatumdimorphism, regulation by cAMP, 1452

Homology between plasmids ColEl and p15A, 1305hut genes

K. aerogenes, 664S. typhimurium, 664

Hydrogenase formationA. eutrophus, 1144nickel requirement, 1144

Hydrophobic, photolabile probe bindingE. coli, 341membrane potential, 341

HyphaeC. laurentii, 1410septal complex, 1410

ilvGEDA operonS. typhimurium, 984

Inclusions containing Chiamydia psittaci, 605Inner membraneH. influenzae, encapsulated, 596

InosineB. subtilis sporulation, 489

Isocitrate lyaseYersinia species, 452

K88ac adherence antigenE. coli, 920identification and cloning, 920

Kasugamycin resistance geneE. coli, 914ksgD, 914

Killer characterK. lactis, 382plasmid associated, 382

Klebsiella aerogenesaromatic amino acid aminotransferase, 266aromatic amino acid regulation, 257hut genes, 664tryptophan metabolism, 257

Klebsiella pneumoniaemolybdenum accumulation and storage, 743nif-lac fusions, 348nif regulation, 1116nitrogenase synthesis, 1116nitrogen fixation, 348

Klebsiella sp. strain CT-1

x SUBJECT INDEX

lactose transport, 1459Kluyveromyces lactis

linear DNA plasmids, 382ksgB

E. coli, 914ksgD

E. coli, 914Kynurenine hydroxylase

S. cerevisiae mitochondria, 1325

lac operonE. coli, 410relAU gene, 410

fB-Lactamase gene (penG)cloning in E. coli and B. subtilis, 422

Lactobacillusplantarummanganese, 442oxygen toxicity, 442

Lactose transportKlebsiella sp. strain CT-1, 1459

Lectinsymbiotic relationship between bacteria and

sponges, 548Lectin binding

polysaccharide, 1063R. japonicum, 1063

Leucine incorporationB. thetaiotaomicron, 8phospholipid, 8

L-formB. subtilis, 651growth parameters in batch culture, 651

Linear deoxyribonucleic acid plasmidsK. lactis, 382

Lipid metabolismA. vinelandii encystment, 889

Lipid synthesisE. coli cell cycle, 472

Lipopolysaccharideheptose region, 138R. tenue, 138

Liquid holding recoveryB. fragilis, 1oxygen effect, 1

Ippdeletion mutations selected by globomycin, 654E. coli, 654

Lysinphage A25 virulent for group A streptococci, 696Streptococcus group C phage lysin, 661

Macrolide-lincosamide-streptogramin B resistanceB. licheniformis, 129

Malate synthaseYersinia species, 452

ManganeseL. plantarum, 442oxygen toxicity, 442

Mannitol enzyme IIS. typhimurium, 1106

M. barkeri, 27Membrane

E. coli, 1293penicillin-binding proteins, 1293

phospholipid transfer, 1154R. sphaeroides, 1154

Membrane, innerH. influenzae, encapsulated, 596

Membrane, outercitrate-Tris-mediated release, 1386E. coli 08, 1386, 1397H. influenzae, encapsulated, 596Tris modification of permeability, 1397

Membrane fluidityS. cerevisiae mitochondria, 1325

Membrane fractionsR. capsulata grown chemotrophically and photo-

trophically, 1121Membrane potential

E. coli, 341hydrophobic, photolabile probe binding, 341

Membrane proteinchloramphenicol, 417P. aeruginosa, 417puromycin, 417rifampin, 417S. citri, 61streptomycin, 417tetracycline, 417

Menaquinone biosynthesisB. subtilis, 321men gene clustering, 321men mutants, 321, 328

men gene clusteringB. subtilis, 321

men mutantsB. subtilis, 321, 328menaquinone biosynthesis, 321

2-Mercaptoethanesulfonic acid derivativesM. barkeri, 27methane formation, 27

Methanosarcina barkericoenzyme M derivatives, 27methane formation, 27

Methyl-accepting chemotaxis proteinsE. coli, 35, 43III, 43multiple forms, 35trg, 43

5-MethylanthranilateE. coli mutants, 1334

Methylation, chemotacticB. subtilis, 958

5-MethyltryptophanE. coli mutants, 1334

Micrococcus variansteichoic acid biosynthesis, 1222

Mismatch correctionE. coli, 644

Mitochondriakynurenine hydroxylase, 1325membrane fluidity, 1325S. cerevisiae, 1325sterol composition, 1325

MLS resistanceB. licheniformis, 129

MolybdateA. vinelandii, 248

J. BACTERIOL.

VOL. 145, 1981

nitrogenase activation, 248Molybdenum accumulation and storageA. vinelandii, 743K. pneumoniae, 743

mot mutantsS. typhimurium, 1082

Mucor rouxiicell wall synthesis, 272

Multicopy extragenic suppressors of dnaA mutationsE. coli, 861

Murein lipoproteinE. coli, 657globomycin, 657S. typhimurium, 657

Murein synthesisE. coli, 333penicillin-binding protein, 333

Mycoplasma gallisepticumcell volume regulation, 1299proton motive force across membrane, 1299

Myxococcus xanthusgenome size, 1257

Neisseria gonorrhoeaecell envelope, 946DNA modifications, 788double-stranded DNA entry during transformation,

638outer membrane proteins, 736oxygen uptake, 946restriction endonuclease production, 788steroid hormones, 946

Neisseria meningitidisL-cysteine oxidase, 280

Neurospora crassaD-arabinose dehydrogenase, 164gin-lb mutation, 884nitrogen metabolite repression, 884polyamine synthesis, 14putrescine pool, 14spermidine pool, 14

NIL+nitrogenase, 824R. rubrum, 824

Nickel requirementA. eutrophus, 1144hydrogenase formation, 1144

Nicotinamide adenine dinucleotide glycohydrolaseS. typhimurium, 1002

Nicotinamide adenine dinucleotide pyrophosphataseS. typhimurium, 1002

Nicotinamide mononucleotide glycohydrolaseS. typhimurium, 1002

nifK. pneunwniae, 1116

nif genesRhizobium plasmids, 1129

nif-lac fusionsK. pneumoniae, 348nitrogen fixation, 348

Nitrate reductaseAnabaena sp. strain 7119,175A. nidulans, 175Nostoc sp. strain 6719, 175

SUBJECT INDEX xl

Nitrogenase activationA. vinelandii, 248molybdate, 248

Nitrogenase activityNH4+, 824R. rubrum, 824

Nitrogenase synthesisK. pneumoniae, 1116temperature sensitivity of regulation, 1116

Nitrogen fixationK. pneumoniae, 348nif-lac fusions, 348

Nitrogen metabolismB. subtilis, 768glutamine, 768

Nitrogen metabolite repressiongin-lb, 884N. crassa, 884

Nostoc sp. strain 6719nitrate reductase, 175

NucleoidB. subtilis, 1177DNA fragment assimilation, 1177

Nucleoid repairE. coli, 1456thermal damage, 1456

NucleosideB. subtilis sporulation, 489

O-haptenS. montevideo, 624

LL-OligopeptidaseB. sphaericus, 675

OmpC, OmpFE. coli, 88regulatory mutations, 88

OmpF proteinE. coli, 1085phage lambda carrying gene for, 1085

oriC-dependent duplex deoxyribonucleic acid replica-tion, 974

O-Succinylbenzoyl-coenzyme A synthetaseB. subtilis men mutants, 328

Outer membranecitrate-Tris-mediated release, 1386E. coli 08, 1386, 1397H. influenzae, encapsulated, 596Tris modification of permeability, 1397

Outer membrane proteinE. coli, 88OmpC, 88OmpF, 88P. aeruginosa, 628peptidoglycan associated, 628regulatory mutations, 88V. cholerae, 1018

Outer membrane proteinsN. gonorrhoeae, 736

oxaE. coli, 808transposition, 808

Oxacillin-hydrolyzing penicillinase geneE. coli, 808oxa, 808

xrii SUBJECT INDEX

Oxygen effectB. fragilis, 1liquid holding recovery, 1

Oxygen toxicityL. plantarum, 442manganese, 442

Oxygen uptakeN. gonorrhoeae, 946steroid hormones, 946

Parasporal crystalsB. thuringiensis, 1052

penGcloning in E. coli and B. subtilis, 422

Penicillin-binding proteinE. coli, 333, 632furazlocillin, 632septal murein synthesis, 333

Penicillin-binding protein 2E. coli, 398

Peniciflin-binding proteinsE. coli membrane, 1293

Peptide chain elongation factor GE. coli, 429ppGpp synthesis, 429

Peptidoglycancadaverine linkage, 122corynebacteria, 775S. ruminantium, 122

Peptidoglycan associationouter membrane protein, 628P. aeruginosa, 628

Peptidoglycan cross-linkageD-alanine carboxypeptidase, 845E. coli, 845

Periplasmic-leaky mutantsE. coli, 1351

Pesticide degradationA. eutrophus, 681A. paradoxus, 681plasmids pJP3, pJP4, pJP5, pJP7, pJP2, and pJP9,

681pgsA

E. coli, 113phosphatidylglycerol synthesis, 113

pgsBE. coli, 113phosphatidylglycerol synthesis, 113

pH, cytoplasmicE. coli, 1209pH taxis, 1209Salmonella, 1209weak-acid repellent taxis, 1209

pH, intracellularchemotaxis, 1196E. coli, 1196

phoAE. coli, 288, 293

PH082-pho4phosphatase regulation, 221S. cerevisiae, 138

phoS24(Am)E. coli, 1249phosphate-specific transport, 1249

J. BACTERIOL.

PhosphateCoIB2 pilin, 1167EDP208 pilin, 1167

Phosphate regulationPH082-pho4, 221S. cerevisiae, 221

Phosphate-specific transportE. coli mutants, 1249

Phosphatidylglycerol synthesisE. coli, 113pgsA, 113pgsB, 113

PhospholipidB. thetaiotamicron, 8intermembrane transfer, 1154leucine incorporation, 8R. sphaeroides, 1154

Phospholipid biosynthesisC. crescentus stalk elongation, 1404

phoT35E. coli, 1249phosphate-specific transport, 1249

PiliB. cereus spores, 613H. parainfluenzae, 1075

Pilincarbohydrate, 1167ColB2, 1167EDP208, 1167phosphate, 1167

Plasmid ColElE. coli, 1305homology with pl5A, 1305

Plasmid detection and isolation, 1365Plasmid 2,u DNA

curing, 1421S. cerevisiae, 1421

Plasmid pl5AE. coli, 1305homology with ColEl, 1305

Plasmid pAB124tetracycline accumulation in B. subtilis and E. coli,

1417Plasmid pBF4

B. fragilis, 867Plasmid pC194

transformation of B. thuringiensis protoplasts, 980Plasmid pCG86

E. coli, 97elt, 97enterotoxin production, 97estA, 97mercury resistance, 97streptomycin resistance, 97sulfonamide resistance, 97tetracycline resistance, 97

Plasmid pCR1intrastrand base pairing in single-stranded DNA,

1436Plasmid pHlyl52

E. coli, 233Plasmid pIP646

streptococci groups C and G, 1102Plasmid pIP920

VOL. 145, 1981

streptococci groups C and G, 1102Plasmid pKM101

functional organization, 1310Plasmid pMO79

P. aeruginosa, 1448Plasmid RP1

E. coli RP1-Hfr strain formation, 713Plasmid RP4::Mu cts

expansion of phage Mu host range, 358PlasmidsAgrobacterium app., 1365Erwinia spp., 1365Escherichia spp., 1365halobacteria, 369nif genes, 1129Pseudomonas spp., 1365rhizobia, 1129Rhizobium spp., 1365Sabnonella spp., 1365Xanthomonas app., 1365

Plasmids pJP2-pJP5, pJP7, pJP9A. eutrophus, 681A. paradoxus, 681pesticide degradation, 681

Plasmid transferprotoplast fusion in staphylococci 74R. japonicum, 1025

Podospora spp.ribosomal protein genes, 82

Polar morphogenesisC. crescentus, 559

Polyamine synthesisN. crassa, 14putrescine pool, 14spermidine pool, 14

Polypeptide PsB. subtilis chromosomes, 953

Polyporus circinatusa-glucan degradation, 171glucose, 171

Polysaccharidelectin binding, 1063R. japonicum, 1063

Postreplication repairB. subtilis, 434UV-irradiated transforming DNA, 434

Proline metabolismregulation by compartmentation, 1359S. cerevisiae, 1359

ProteinS. citri membrane, 61

Protein AE. coli, 513

Protein B56.5E. coli, 513

Protein synthesis, ribosomalE. coli mutant missing ribosomal protein Li, 1445

Proton motive forcecell volume regulation, 1299M. gallisepticum membrane, 1299

Protoplast fusionplasmid transfer, 74recombination, 74S. aureus, 74

SUBJECT INDEX Xiii

Pseudomonad relatednessallostery of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase, 752

Pseudomonas aeruginosacAMP level, 1286chloramphenicol, 417chromosome, genetic circularity of, 145cloning vector, 1448histidase level, 1286membrane proteins, 417outer membrane protein, 628peptidoglycan, 628plasmid pMO79, 1448puromycin, 417rifampin, 417streptomycin, 417tetracycline, 417

Pseudomonas nsolitalectin, 548symbiosis with H. panicea, 548

Pseudomonas putidacAMP level, 1286histidase level, 1286

Pseudomonas spp.plasmid detection and isolation procedure, 1365

Pseudomonas sp. strain C12Barylsulfatase, 1428

pst-2E. coli, 1249phosphate-specific transport, 1249

Puromycinmembrane proteins, 417P. aeruginosa, 417

Putrescine poolN. crassa, 14polyamine synthesis, 14

Pyridine nucleotide cycleNAD glycohydrolase, 1002NAD pyrophosphatase, 1002nicotinamide mononucleotide glycohydrolase, 1002S. typhimurium, 1002

pyr.:lac fusionsS. typhimurium infected with Mu dl (Apr lac), 299

PyrophosphateDesulfotomaculum spp., 966Desulfovibrio spp., 966sulfate reduction, 966

RecE pathway of genetic recombinationE. coli, 521

RecombinationB. subtilis, 1177DNA fragment assimilation, 1177EDTA, 1189H. influenzae, 1189phage lambda, 306protoplast fusion in staphylococci, 74

relAE. coli, 410lac operon, 410

relA+ geneE. coli, 410lac operon regulation, 410

rel defect

Xiv SUBJECT INDEX

E. coli, 641f8-galactosidase synthesis, 641

Restriction endonucleaseS. aureofaciens, 873

Restriction endonuclease productionN. gonorrhoeae, 788

Rhizobiaplasmids, 1129

Rhizobiumjaponicumchemoautotrophic growth with H2, 533lectin binding, 1063plasmid transfer, 1025polysaccharide, 1063

Rhizobium spp.plasmid detection and isolation procedure, 1365

Rhodopseudomonas capsulatamembrane fractions from chemotrophically and

phototrophically grown cells, 1121Rhodopseudomonas sphaeroides

phospholipid transfer, 1154Rhodospirillum rubrumammonium ions, 824nitrogenase activity, 824

Rhodospirillum tenueheptose region, 138lipopolysaccharide, 138

Ribonucleic acid polymerase inhibitorsB. brevis DNA replication, 1442

Ribosomal protein genesPodospora spp., 82

Ribosomal protein synthesisE. coli mutant missing ribosomal protein Li, 1445

Ribosome synthesisS. cerevisiae ascosporogenesis, 1342

Rifampinmembrane proteins, 417P. aeruginosa, 417

Rifampin resistance mutationsE. coli, 1334transcription termination, 1334

R plasmidsstreptococci groups C and G, 1102

Saccharomyces cerevisiaearginine metabolism, 1359compartmentation in control of converging path-ways for proline and arginine metabolism, 1359

kynurenine hydroxylase, 1325membrane fluidity, 1325mitochondria, 1325PH082-pho4, 221phosphatase regulation, 221plasmid 2,u DNA, 1421proline metabolism, 1359ribosome synthesis during ascosporogenesis, 1342sinefungin, 106sterol composition, 1325sterol transmethylation, 106

Saccharomycopsis lipolyticaalkaline extracellular protease, 404XPR2, 404

Salmonellachemotaxis, 1209cytoplasmic pH, 1209

J. BACTERIOL.

Salmonella montevideoO-hapten, 624

Salmonella spp.plasmid detection and isolation procedure, 1365

Salmonella typhimuriumaerotaxis, 990arylsulfatase, 796carbamyl aspartate, 1095coliphage Mu dl (Ap' lac) infection, 299electron transport, 9905-fluorouracil resistance, 1095globomycin, 657hut genes, 664ilvGEDA operon, 984mannitol enzyme II, 1106murein lipoprotein, 657NAD glycohydrolase, 1002NAD pyrophosphatase, 1002nicotinamide mononucleotide glycohydrolase, 1002passive rotation of flagella on paralyzed (mot) mu-

tants, 1082pyridine nucleotide cycle, 1002pyr.:lac fusions, 299tyramine oxidase, 796ubiquinone biosynthesis, 1095

Selenomonas ruminantiumpeptidoglycan, 122

Septal murein synthesisE. coli, 333penicillin-binding protein, 333

sfrA and sfrB products'E. coli, 840transcriptional control factors, 840

SinefunginS. cerevisiae, 106sterol transmethylation, 106

Spermidine poolN. crassa, 14polyamine synthesis, 14

SpiralinS. citri membrane, 61

Spiroplasma citrimembrane proteins, 61spiralin, 61

Spongelectin, 548symbiosis with P. insolita, 548

Spore coat proteinB. cereus, 541

SporesB. cereus, 613pili, 613

SporogenB. subtilis, 489inosine, 489

SporulationB. sphaericus, 675B. subtilis, 489, 1281inosine, 489nucleosides, 489LL-oligopeptidase, 675phage PMB12, 1281sporogen, 489

Staphylococcus aureus

VOL. 145, 1981

plasmid transfer, 74protoplast fusion, 74recombination, 74teichoic acid biosynthesis, 1222transposon Tn551 insertion sites, 479

Steroid hormonesN. gonorrhoeae, 946oxygen uptake, 946

Sterol compositionS. cerevisiae mitochondria, 1325

Sterol transmethylationS-adenosylhomocysteine analogs, 106S. cerevisiae, 106

Stigmatella aurantiacagenome size, 1257

Streptococciphage lysin, 696

Streptococci groups C and GR plasmids, 1102

Streptococcus faecalistransposon Tn916, 494

Streptococcus group C phage lysin, 661Streptomyces aureofaciens

restriction endonuclease, site-specific, 873SauI, 873

Streptomyces chartreusisendo-a-1,3-glucanase, 729

Streptomycinmembrane proteins, 417P. aeruginosa, 417

Succinic semialdehyde dehydrogenasesE. coli grown on y-aminobutyrate, 1425

Sulfate reductionDesulfotomaculum spp., 966Desulfovibrio spp., 966pyrophosphate, 966

Suppressor efficiencyE. coli, 704tRNA 'n Su+2 mutants, 704

Symbiosisbacteria and sponges, 548lectin, 548

Teichoic acid biosynthesisB. subtilis, 1222main chain attachment, 1222M. varians, 1222S. aureus, 1222

Temperature-sensitive suppressionE. coli amber mutations, 459

Tetracyclinemembrane proteins, 417P. aeruginosa, 417

Tetracycline resistanceE. coli, 1110medium for selection of clones, 1110S. faecalis, 494

Tetracycline resistance genesplasmid pAB124, 1417tetracycline accumulation in B. subtilis and E. coli,

1417Threonine

carbon source for E. coli, 1150Threonine deaminase

SUBJECT INDEX xv

cysteine, 1031E. coli, 1031growth inhibition, 1031

tmsl2B. subtilis, 1042

TOL genes xylB and xylEcloning, 1137E. coli, 1137

toxDNA sequences, repeated, 850E. coli, 850

Transcriptional controlE. coli 840sfrA and sfrB products, 840

Transcription terminationE. coli, 1334rifampin resistance mutations, 1334tryptophan operon attenuator, 1334

Transfer ribonucleic acid mutantsamber suppression, 704

TransformationB. subtilis L-form, 878B. thuringiensis protoplasts, 980DNA binding, 780E. coli, 780H. influenzae, 1099phage 425 and 029 DNA, 878plasmid pC194, 980

Transforming deoxyribonucleic acidB. subtilis, 434postreplication repair, 434UV irradiation, 434

TransphosphorylationE. coli, 391enzymes II, 391

Transposon Tn916"conjugal" transfer, 494S. faecalis, 494

Transposon Tn551 insertion sitesS. aureus, 479

trgchemotaxis protein III, methyl-accepting, 43E. coli, 43

4,5',8-Trimethylpsoralen monoadductsB. subtilis, 832transforming DNA, 832

Tris(hydroxymethyl)aminomethaneE. coli 08, 1386, 1397outer membrane permeability, 1397outer membrane release, 1386

Tryptophan metabolismaromatic amino acid regulation, 257K. aerogenes, 257

Tryptophan operon attenuatorE. coli rpoB mutations, 1334

tslB. subtilis, 1042

tsl2B. subtilis, 1042

Tyramine oxidasearylsulfatase derepressed synthesis, S. typhimu-

rium, 796

Ubiquinone biosynthesis

xvi SUBJECT INDEX

carbamyl aspartate, 10955-fluorouracil resistance, 1095ubiquinone biosynthesis, 1095

Ultraviolet irradiationB. subtilis, 434C. albicans, 896postreplication repair, 434transforming DNA, 434

uncBadenosine triphosphate complex, Fo portion, 200E. coli, 200

uncEadenosine triphosphatase complex, Fo portion, 200E. coli, 200

uncFadenosine triphosphate complex, Fo portion, 200E. coli, 200

Uracil-containing deoxyribonucleic acidE. coli, 687

Uracil incorporationphage lambda DNA, 306

uxu operonE. coli, 211regulation, 211

uxuR gene productE. coli, 211uxu operon regulation, 211

Vi antigen expression

J. BACTERIOL.

C. freundii, 1010genetic regulation, 1010

Vibrio choleraeouter membrane protein, 1018

Vitamin B12Bacteroides species, 466

Wall turnoverB. subtilis, 50

Xanthomonas spp.plasmid detection and isolation procedure, 1365

XPR2alkaline extracellular protease, 404S. lipolytica, 404

xylBcloning, 1137E. coli, 1137

xylEcloning, 1137E. coli, 1137

Yersinia pestisisocitrate lyase, multiple forms of, 452

Yersinia speciesglyoxylate bypass enzymes, 452isocitrate lyase, 452malate synthase, 452

JOURNAL OF BACTERIOLOGYVOLUME 145 0 1981

EDITORIAL BOARD

Stanley C. Holt, Editor (1982)University of Massachusetts, Amherst

Samuel Kaplan, Editor (1983)University of Illinois, Urbana

June J. Lascelles, Editor (1984)University of Cal!fornia, Los Angeles

Mark Achtman (1982)James Akagl (1982)David Apirion (1982)Arthur I. Aronson (1982)Gad Avigad (1983)Stephen D. Barbour (1982)Manfred E. Bayer (1982)Claire M. Berg (1983)Robert W. Bernobr (1982)Terry J. Beveridge (1982)Dale C. Birdsell (1981)Edwin Boatman (1983)Winfried Boos (1982)H. D. Braymer (1982)Jean Brenchley (1983)Patrick J. Brennan (1981)Robert P. Burchard (1982)1. D. J. Burdett (1982)Richard 0. Burns (1983)Ercole Canale-Parola (1982)A. M. Chakrabarty (1983)Murray S. Cohn (1982)John W. Costerton (1983)Antbony R. Crofts (1982)John E. Cronan, Jr. (1983)Lolita Daneo-Moore (1981)Daniel V. Der Vartanian (1982)Walter J. Dobrogosz (1982)W. D. Donachie (1982)W. Ford Doolittle (1982)J. Allan Downie (1982)Gerhart Drews (1982)Charles Earhart (1981)A. Elsenstark (1982)David P. Fan (1981)Walton L. Faogman (1981)John D. Foulds (1982)

Simon Silver, Editor-in-Chief (1982)Washington University, St. Louis, Mo.

Elizabeth McFall, Editor (1985)New York University, New York, N. Y.Donald P. Nierlich, Editor (1982)

University of Cal!fornia, Los AngelesAllen T. Philips, Editor (1985)Pennsylvania State University,

University Park

Ann Ganesan (1982)J. F. Gardner (1981)Robert Gennis (1982)Bijan K. Ghosh (1981)David T. Gibson (1981)Harry E. Gilleland, Jr. (1982)Patricia L. Grilione (1982)Walter R. GuiLd (1981)Tadayo Hashimoto (1982)Gerald L. Hazelbauer (1981)Charles E. Helmstetter (1982)Ulf Henning (1982)Peter Hirsch (1982)Bruce Holloway (1982)Philip Hylemon (1982)Karin Ihler (1981)Joseph Inselburg (1981)Robert J. Kadner (1982)Eva R. Kashket (1981)Donald L. Keister (1983)Edward Keilenberger (1982)David E. Kennel (1983)Jordan Konisky (1981)K. W. Knox (1982)Thomas G. Lessie (1982)Donald R. Lueking (1981)Ben J. J. Lugtenberg (1983)Carolyn MacGregor (1981)Manley Mandel (1981)Barry Marrs (1981)Ann Matthysse (1982)Neil H. Mendelson (1981)Sally Ann Meyer (1981)Robert V. Miller (1981)David Mount (1982)R. G. E. Murray (1983)Walter G. Niehaus, Jr. (1981)Hiroshi Nikaido (1981)

Robert Rownd, Editor (1985)Northwestern Medical School

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JOURNAL

OF

BACTERIOLOGY

VOLUME 145

WASHINGTON, DC 20006

1981

Volume 145 Contents for January 1981Morphology and Ultrastructure

Structure of the Heptose Region of Lipopolysaccharides from Rho-dospirillum tenue. JOANNA RADZIEJEWSKA-LEBRECHT, U.FEIGE, H. MAYER,* AND J. WECKESSER .... ................ 138-144

Regulation ofPolar Morphogenesis in Caulobacter crescentus. AKIoFUKUDA,* MAKOTO ASADA, SHIGEO KOYASU, HIDEYA YOSHIDA,KATSUYUKI YAGINUMA, AND YOSHI OKADA .... ............. 559-572

Isolation and Electron Microscopic Observations of Intracyto-plasmic Inclusions Containing Chlamydia psittaci. AKIRAMATSUMOTO ............................................. 605-612

Isolation and Properties of Pili from Spores ofBacillus cereus. JOHNP. DEsRoSIER AND J. CANO LARA* .... ..................... 613-619

General MicrobiologyLectin, a Possible Basis for Symbiosis Between Bacteria and

Sponges. WERNER E. G. MULLER,* RUDOLF K. ZAHN, BRANDOKURELEC, CEDOMIL Lucu, ISABEL MULLER, AND GERDUHLENBRUCK............................................ 548-558

Quantitation of Bacillus subtilis L-Form Growth Parameters inBatch Culture. RICHARD W. GILPIN,* SUZANNE K. PATTER-SON, AND RALPH A. KNIGHT ............................... 651-653

Plant MicrobiologyElaboration of Cellulose Fibrils by Agrobacterium tumefaciens Dur-

ing Attachment to Carrot CelLs. ANN G. MATTHYSSE,* KATH-RYN V. HOLMES, AND ROBIN H. G. GURLITZ .... ............. 583-595

Genetics and Molecular BiologyMethyl-Accepting Chemotaxis Protein III and Transducer Gene

trg. GERALD L. HAZELBAUER,* PETER ENGSTROM, AND SHI-GEAKi HARAYAMA ..................... 43-49

Stringent Response of Bacillus stearothermophilus: Evidence forthe Existence of Two Distinct Guanosine 3',5'-PolyphosphateSynthetases. SUSANNE FEHR AND DIETMAR RICHTER* ...... 68-73

Plasmid Transfer and Genetic Recombination by Protoplast Fusionin Staphylococci. F. GOTZ, S. AHRNE, AND M. LINDBERG* ... 74-81

Naturally Occurring Macrolide-Lincosamide-Streptogramin B Re-sistance in Bacillus licheniformis. A. DOCHERTY, G. GRANDI,R. GRANDI, T. J. GRYCZAN, A. G. SHIVAKUMAR, AND D.DUBNAU* .............................. ....... ........ 129-137

Genetic Circularity of the Pseudomonas aeruginosa PAO Chromo-some. P. L. ROYLE, H. MATSUMOTO, AND B. W. HOLLOWAY* 145-155

Molecular Cloning of Escherichia coli K-12 Hexuronate SystemGenes: exu Region. PAUL RITZENTHALER,* MIREILLE MATA-GILSINGER, AND FRANVOIS STOEBER .... ................... 181-190

Three Genes Coding for Subunits of the Membrane Sector (F0) ofthe Escherichia coli Adenosine Triphosphatase Complex. J.ALLAN DOWNIE,* GRAEME B. Cox, LYNDALL LANGMAN, GREGASH, MICHAEL BECKER, AND FRANK GIBSON ... ............. 200-210

Regulation of Hexuronate System Genes in Escherichia coli K-12:Multiple Regulation of the uxu Operon by exuR and uxuR GeneProducts. JANINE ROBERT-BAUDOUY,* RAYMOND PORTALIER,AND FRANCOIS STOEBER

.................................. 211-220

* Asterisk refers to person to whom inquiries regarding the paper should be addressed.

Number 1

CONTENTS

Deletion Map of the Escherichia coli Structural Gene for AlkalinePhosphatase, phoA. APARNA SARTHY, SUSAN MICHAELIS,AND JON BECKWITH* ............ ......................... 288-292

Use of Gene Fusions to Determine the Orientation of Gene phoA onthe Escherichia coli Chromosome. APARNA SARTHY, SUSANMICHAELIS, AND JON BECKWITH* ....... ................... 293-298

Infection of Salmonella typhimurium with Coliphage Mu dl (Aprlac): Construction of pyr::lac Gene Fusions. LAszL6 N.CSONKA, MARTHA M. HOWE, JOHN L. INGRAHAM, LELAND S.PIERSON III, AND CHARLES L. TURNBOUGH, JR.* .... ........ 299-305

Recombination ofUracil-Containing Lambda Bacteriophages. JOHNB. HAYS,* BRUCE K. DUNCAN, AND SIEGHILD BOEHMER ..... 306-320

Menaquinone Biosynthesis in Bacillus subtilis: Isolation of menMutants and Evidence for Clustering ofmen Genes. HARRY W.TABER,* ELIZABETH A. DELLERS, AND LINDA RIVOIRELOMBARDO .............................................. 321-327

Regulation of Nitrogen Fixation in Klebsiellapneumoniae: Isolationand Characterization of Strains with nif-lac Fusions. DOUGLASMACNEIL, JIABI ZHU, AND WINSTON J. BRILL* .... .......... 348-357

Genetic Variability in Halobacteriupz halobium. F. PFEIFER,* G.WEIDINGER, AND W. GOEBEL ........ ...................... 375-381

In Vivo Role of the reLA + Gene in Regulation of the lacOperon. PAUL PRIMAKOFF ........ ....................... 410-416

Role of Peptide Chain Elongation Factor G in Guanosine 5'-Diphos-phate 3'-Diphosphate Synthesis. CHIA C. PAO, * JIM FLECK-ENSTEIN, AND BARBARA T. DYESS ....... .................. 429-433

Postreplication Repair of Ultraviolet-Irradiated Transforming De-oxyribonucleic Acid in Bacillus subtilis. CHARLES T.HADDEN ................................................. 434-441

High-Efficiency, Temperature-Sensitive Suppression of Amber Mu-tations in Escherichia coli. JANICE M. ZENGEL* AND LASSELINDAHL ................................................ 459-465

Identity of the B56.5 Protein, the A-Protein, and the groE GeneProduct ofEscherichia coli. FREDERICK C. NEIDHARDT,* TER-ESA A. PHILLIPS, RUTH A. VANBOGELEN, MARTIN W. SMITH,YANNIS GEORGALIS, AND ALAP R. SUBRAMANIAN .... ........ 513-520

Genetic Analysis of the RecE Pathway of Genetic Recombination inEscherichia coli K-12. JANE R. GILLEN, DAVID K. WILLIS,AND ALVIN J. CLARK* .................................... 521-532

Biological Consequences of Infection of Escherichia coli B by Al-kylated T7 Bacteriophage. BARBARA KARSKA-WYSOCKi* ANDMARGARET D. MAMET-BRATLEY ...... ..................... 573-582

Entry of Double-Stranded Deoxyribonucleic Acid During Transfor-mation of Neisseria gonorrhoeae. GOUR D. BISWAS* AND P.FREDERICK SPARLING ........... .......................... 638-640

Mechanism of the rel Defect in fi-Galactosidase Synthesis. DIANEFOLEY, PATRICK DENNIS, AND JONATHAN GALLANT* .... ..... 641-643

Escherichia coli K-12 Clones That Overproduce dam Methylase AreHypermutable. GAIL E. HERMAN AND PAUL MODRICH* ..... 644-646

Use of cir-lac Operon Fusions to Study Transcriptional Regulationof the Colicin Ia Receptor in Escherichia coli K-12. PATRICIAL. WORSHAM AND JORDAN KONISKY* ...... ................. 647-650

CONTENTS

Physical Maps of Klebsiella aerogenes and Salmonella typhimuriumhut genes. MIROsLAv BLUMENBERG AND BORIS MAGASANIK* 664-667

Plasmids and TransposonsStructure of a Naturally Occurring Plasmid with Genes for Entero-

toxin Production and Drug Resistance. ANTHONY J. MAZAITIS,RENATA MAAS, AND WERNER K. MAAS* .................... 97-105

Determination of the Functions of Hemolytic Plasmid pHlyl52 ofEscherichia coli. ANGELIKA NOEGEL, URSULA RDEST, ANDWERNER GOEBEL* ....................................... 233-247

Introduction of Bacteriophage Mu into Bacteria of Various Generaand Intergeneric Gene Transfer by RP4::Mu. YOSHIKATSU MU-ROOKA,* NOBORU TAKZAWA, AND TOKUYA HARADA .... ..... 358-368

Characterization of Plasmids in Halobacteria. F. PFEIFER,* G. WEI-DINGER, AND W. GOEBEL ................. . .... .. .... .... 369-374

Molecular Cloning and Expression of Bacillus licheniformis /3-Lac-tamase Gene in Escherichia coli and Bacillus subtilis. OANHGRAY AND SHING CHANG* ................................ 422-428

Distribution of Tn551 Insertion Sites Responsible for Auxotrophy on

the Staphylococcus aureus Chromosome. PETER A. PATTEE 479-488Evidence for a Chromosome-Borne Resistance Transposon (Tn916)

in Streptococcus faecalis That Is Capable of "Conjugal" Trans-fer in the Absence of a Conjugative Plasmid. ARTHUR E.FRANKE AND DON B. CLEWELL*.......... 494-502

Eucaryotic CellsRegulation of Polyamine Synthesis in Relation to Putrescine and

Spermidine Pools in Neurospora crassa. THOMAS J. PAULUSAND ROWLAND H. DAVIS* .............. ....... ... 14-20

Genetics of Ribosomal Components in Podospora spp. as Revealedby Interspecific Hybridizatin. MICHEL PERROT* AND JOELBEGUERET ............................................... 82-87

Inhibition of Sterol Transmethylation by S-AdenosylhomocysteineAnalogs. MARK T. MCCAMMON AND L. W. PARKS* .... ..... 106-112

Genetic and Biochemical Characterization of D-Arabinose Dehydro-genase from Neurospora crassa. ALICIA CARRASCO,* GUIDOPINCHEIRA, AND TITO URETA .............................. 164-170

Effect of Glucose on ci-Glucan Degradation in Polyporus circina-tus. MARIA B. M. OLIVEIRA AND GLACI T. ZANCAN* ........ 171-174

Structure and Function of the PH082-pho4 Locus Controlling theSynthesis of Repressible Acid Phosphatase of Saccharomycescerevisiae. AKIO TOH-E,* S. INOUYE, AND Y. OSHIMA 221-232

Role of Membranes of Mycelial Mucor rouxii in Synthesis andSecretion of Cell Wall Matrix Polymers. J. MAXWELL Dow,ROBERTO R. CARREON, AND VICENTE D. VILLA* .... ........ 272-279

Isolation and Characterization of Linear Deoxyribonucleic Acid Plas-mids from Kluyveromyces lactis and the Plasmid-AssociatedKiller Character. NORIo GUNGE,* ATSUKO TAMARU, FUMIKOOZAWA, AND KENJI SAKAGUCHI ....... ..................... 382-390

Structural Gene for the ALkaline Extracellular Protease of Sacchar-omycopsis lipolytica. PAULA C. SIMMS AND DIVID M.OGRYDZIAK*............................................. 404-409

CONTENTS

MembranesMultiple Forms of Methyl-Accepting Chemotaxis Proteins Distin-

guished by a Factor in Addition to Multiple Methylation.GERALD L. HAZELBAUER* AND PETER ENGSTROM .... ....... 35-42

Electrophoretic Analysis of the Arrangement of Spiralin and OtherMajor Proteins in Isolated Spiroplasma citri Cell Membranes.HENRI WR6BLEWSKI ..................................... 61-67

Regulatory Mutations Conferring Constitutive Synthesis of MajorOuter Membrane Proteins (OmpC and OmpF) in Escherichiacoli. TAKASHI SATO AND TAKASHI YURA* ..... ............ 88-96

Two Interacting Mutations Causing Temperature-Sensitive Phos-phatidylglycerol Synthesis in Escherichia coli Membranes.MASAHIRO NISHIJIMA, CHRISTINE E. BULAWA, AND CHRISTIANR. H. RAETZ* ............................................. 113-121

Cadaverine Is Covalently Linked to Peptidoglycan in Selenomonasruminantium. YOSHIYUKI KAMIO,* YOSHIFUMI ITOH, YOSHIROTERAWAKI, AND TOMONOBU KUSANO ...... ................ 122-128

Ferric Citrate Transport in Escherichia coli Requires Outer Mem-brane Receptor Protein FecA. WILHELM WAGEGG AND VOLK-MAR BRAUN* ............................................ 156-163

Increased Binding of a Hydrophobic, Photolabile Probe to Esche-richia coli Inversely Correlates to Membrane Potential but NotAdenosine 5'-Triphosphate Levels. MARCIA K. WOLF AND JOR-DAN KONISKY* ......... ............... 341-347

Vectorial and Nonvectorial Transphosphorylation Catalyzed by En-zymes II of the Bacterial Phosphotransferase System. MILTONH. SAIER, JR.,* AND MARY R. SCHMIDT ...... ............... 391-397

Biosynthesis of Membrane Proteins of Pseudomonas aeruginosa:Effects of Various Antibiotics. MITSUE YASUMURA, KENZONAKAMURA, DAVID MERNOFF, AND MASAYORI INOUYE* ...... 417-421

Isolation and Partial Characterization ofOuter and Inner Membranesfrom Encapsulated Haemophilus influenzae Type b. MARILYNR. LOEB,* ARTHUR L. ZACHARY, AND DAVID H. SMITH ....... 596-604

Reducing Terminus of O-Hapten Accumulated in a Salmonellamontevideo galE Mutant. FRANKLIN A. HEASLEY .... ...... 624-627

Pseudomonas aeruginosa Outer Membrane: Peptidoglycan-Associ-ated Proteins. ROBERT E. W. HANCOCK,* RANDALL T. IRVIN,J. WILLIAM COSTERTON, AND ALICE M. CAREY .... .......... 628-631

Preferential Selection of Deletion Mutations of the Outer MembraneLipoprotein Gene of Escherichia coli by Globomycin.LAURENCE J. ZWIEBEL, MASATOSKI INUKAI, KENZO NAKAMURA,AND MASAYORI INOUYE* ......... ......................... 654-656

Globomycin Sensitivity of Escherichia coli and Salmonella typhi-murium: Effects of Mutations Affecting Structures of MureinLipoprotein. JIUNU-SHYONG LAI, WILLIAM M. PHILBRICK,SHIGERU HAYASHI, MASATOSHI INUKAI, MAMORU ARAI, YUKI-NORI HIROTA, AND HENRY C. WU* ....... .................. 657-660

Cleavage of Colicin Ia by the Escherichia coli K-12 Outer MembraneIs Not Mediated by the Colicin Ia Receptor. L. K. BOWLESAND JORDAN KONISKY .......... ......................... 668-671

Physiology and MetabolismEffect of Oxygen on Liquid Holding Recovery in Bacteroides fra-

gilis. DAVID T. JONES AND DAVID R. WOODS* ..... .... 1-7

CONTENTS

Incorporation of Leucine into Phospholipids of Bacteroides thetaio-taomicron. RICHARD D. SMITH AND ABIGAIL A. SALYERS* .. 8-13

Coenzyme M Derivatives and Their Effects on Methane Formationfrom Carbon Dioxide and Methanol by Cell Extracts of Meth-anosarcina barkeri. THEO J. HUTTEN, M. HENK DE JONG,BEN P. H. PETERS, CHRIS VAN DER DRIFT, AND GODFRIED D.VOGELS* ...................... 27-34

Cell Wall Turnover in Batch and Chemostat Cultures of Bacillussubtilis. WIM R. DE BOER, FRED J. KRUYSSEN, AND JAN T. M.WOUTERS* ............................................... 50-60

Regulation of Nitrate Reductase Levels in the Cyanobacteria Ana-cystis nidulans, Anabaena sp. Strain 7119, and Nostoc sp. Strain6719. ANTONIA HERRERO, ENRIQUE FLORES, AND MIGUEL G.GUERRERO* ...... ......... ........... 175-180

Microcalorimetric Studies of the Growth of Sulfate-Reducing Bac-teria: Energetics of Desulfovibrio vulgaris Growth. ALFRED S.TRAORE,* CLAUDE E. HATCHIKIAN, JEAN-PIERRE BELAICH, ANDJEAN LE GALL ........................................... 191-199

Tryptophan Metabolism in Klebsiella aerogenes: Regulation of theUtilization of Aromatic Amino Acids as Sources of Nitrogen. C.GREGORY PARIS AND BORIS MAGASANIK* ............... .... 257-265

Identification of Bacillus subtilis men Mutants Which Lack o-Suc-cinyl Benzoyl-Coenzyme A Synthetase and Dihydroxynaph-thoate Synthase. R. MEGANATHAN, RONALD BENTLEY,* ANDHARRY W. TABER ........................................ 328-332

Evidence for Involvement of Penicillin-Binding Protein 3 in MureinSynthesis During Septation but Not During Cell Elongation.GIUSEPPE A. BOTTA AND JAMES T. PARK* ..... ........ 333-340

Purification of Penicillin-Binding Protein 2 of Escherichia coli.SUSAN J. CURTIS AND JACK L. STROMINGER* .... 398-403

Manganese and Defenses Against Oxygen Toxicity in Lacto-bacillus plantarum. FREDERICK S. ARCHIBALD AND IRWINFRIDOVICH* ......... .... .. 442-451

Influence of Heme and Vitamin B12 on Growth and Fermentations ofBacteroides Species. MIN CHEN* AND M. J. WOLIN ... 466-471

Lipid Synthesis During the Escherichia coli Cell Cycle. C. E.CARTY AND L. 0. INGRAM* ................................ 472-478

Induction of Bacillus subtilis Sporulation by Nucleosides: InosineAppears to be Sporogen. VAITHILINGAN SEKAR, SELMA P.WILSON, AND JAMES H. HAGEMAN* ........................ 489-493

Rhizobium japonicum Mutant Strains Unable to Grow Chemoau-totrophically with H2. ROBERT J. MAIER ....... ........... 533-540

Synthesis of Bacillus cereus Spore Coat Protein. ARTHUR I.ARONSON........... 541-547

Divergent Effects of Cyclic Adenosine 3',5'-Monophosphate on For-mation of Type 1 Fimbriae in Different K-12 Strains of Esche-richia coli. BARRY I. EISENSTEIN,* EDWIN H. BEACHEY, ANDSOLOMON S. SOLOMON .................................... 620-623

Effects of Furazlocillin, a fl-Lactam Antibiotic WVhich Binds Selec-tively to Penicillin-Binding Protein 3, on Escherichia coli Mu-tants Deficient in Other Penicillin-Binding Proteins. LAURA S.SCHMIDT, GIUSEPPE BOTTA, AND JAMES T. PARK* ... 632-637

CONTENTS

EnzymologyPurification and Properties of Deoxyribonucleic Acid Polymerase

from Bacillus stearothermophilus. 0. K. KABOEV, L. A.LUCHKINA, A. T. AKHMEDOV, AND M. L. BEKKER* ........... 21-26

In Vitro Activation of Inactive Nitrogenase Component I with Mo-lybdate. PHILIP T. PIENKOS, STEPHEN KLEVICKIS, AND WIN-STON J. BRILL*.248-256

Purification and Properties of Aromatic Amino Acid Aminotrans-ferase from Klebsiella aerogenes. C. GREGORY PARIS ANDBORIS MAGASANIK* ...................................... 266-271

L-Cysteine Oxidase Activity in the Membrane of Neisseria menin-gitidis. E. K. C. YU AND I. W. DEVOE*.. ...... . 280-287

Glyoxylate Bypass Enzymesm' Yersinia Species and Multiple Formsof Isocitrate Lyase in Yersinia pestis. SHARON HILLIER ANDW. T. CHARNETZKY*.452-458

Sequence Homology in the Amino-Terminal and Active-Site Regionsof Thermolabile Glyceraldehyde-3-Phosphate Dehydrogenasefrom a Thermophile. JOHN W. CRABB, ARCHIE L. MURDOCK,TSUNEO SUZUKI, JAMES W. HAMILTON, JAMES H. McLINDEN,AND REMI E. AMELUNXEN .503-512

Purification of Streptococcus Group C Bacteriophage Lysin.JAWAHAR L. RAINA.661-663

ErrataSpontaneous Emergence of an Hfr Strain with a Cit Plasmid from

Natural Isolates of Citrate-Positive Escherichia coli of BovineOrigin. NAOTAKA ISHIGURO, CHIAKI OKA, MASAYOSHI ASAGI,GIHEI SATO, AND MASANOSUKE YOSHIKAWA.672

Regulation of ilvEDA Expression Occurs Upstream of ilvG in Esch-erichia coli: Additional Evidence for an ilvGEDA Operon. C.S. SUBRAHMANYAM, JOHN D. NOTI, AND H. E. UMBARGER ... 673

In Vitro Formation of 8-Galactosidase with a Template Containingthe lac Genes Fused to Gene ilvD. JOHN D. NOTI AND H. E.UMBARGER.673

Morphogenesis of Candida albicans and Cytoplasmic Proteins As-sociated with Differences in Morphology, Strain, or Tempera-tUre. MARCIA MANNING AND THOMAS G. MITCHELL 673

Volume l45 Contents for February 1981 Number 2Morphology and Ultrastructure

Primary Structure of the Wall Peptidoglycan of Leprosy-DerivedCorynebacteria. E. JANCZURA, M. LEYH-BOUILLE, C. COCITO,*AND J. M. GHUYSEN.775-779

Incomplete Flagellar Structures in Escherichia coli Mutants.TAKAHITO SUZUKI AND YOSHIBuMI KOMEDA*.1036-1041

Passive Rotation of Flagella on Paralyzed Salmonella typhimurium(mot) Mutants by External Rotatory Driving Force. AKTRAISHIHARA, SHIGERU YAMAGUCHI, AND HIROKAzu HOTANI* ... 1082-1084

General MicrobiologyOccurrence of Pili on and Adhesive Properties of Haemophilus

parainfluenzae. MARC E. KAHN* AND ROSA GROMKOVA .... 1075-1078

CONTENTS

Plant MicrobiologyLocalization and Characterization of Soybean Lectin-Binding Poly-

saccharide of Rhizobium japonicum. H. C. TSIEN* AND E. L.SCHMIDT .................................... 1063-1074

Genetics and Molecular BiologytRNA2GhI Su+2 Mutants That Increase Amber Suppression. DOUG

BRADLEY,* JAMES V. PARK, AND LARRY SoLL... 704-712Catabolite Repression of Escherichia coli Heat-Stable Enterotoxin

Activity. MARfA GUADALUPE MARTfNEZ-CADENA, Luz MARIAGUZMAN-VERDUZCO, HEATHER STIEGLITZ, AND YANKEL M. Ku-PERSZTOCH-PORTNOY* .. ................ ....... 722-728

Transformation of Escherichia coli with Plasmid DeoxyribonucleicAcid: Calcium-Induced Binding of Deoxyribonucleic Acid toWhole Cells and to Isolated Membrane Fractions. A. WESTON,M. G. M. BROWN, H. R. PERKINS, J. R. SAUNDERS, AND G. 0.HUMPHREYS* ........................... ........ 780-787

Deoxyribonucleic Acid Modifications and Restriction EndonucleaseProduction in Neisseria gonorrhoeae. LENA NORLANDER,*JOHN K. DAVIES, PER HIAGBLOM, AND STAFFAN NORMARK ... 788-795

Inactivation of Transforming Bacillus subtilis DeoxyribonucleicAcid by Monoadducts of 4,5',8-Trimethylpsoralen. HEIN P. J.TE RIELE, PAUL VAN SLUIS, AND GERARD VENEMA* .832-839

Escherichia coli Heat-Labile Enterotoxin Genes Are Flanked byRepeated Deoxyribonucleic Acid Sequences. TATSUO YAMA-MOTO* AND TAKESHI YOKOTA ............................. 850-860

Isolation and Characterization of Multicopy Extragenic Suppressorsof dnaA Mutations. STEVEN J. PROJAN AND JAMES A.WECHSLER* ............... 861-866

Transformation of a Bacillus subtilis L-Form with BacteriophageDeoxyribonucleic Acid. TERRY B. WHITE, RONALD J. DOYLE,AND ULDIS N. STREIPS*.878-883

Isolation and Characterization of Amber Mutations in Gene dnaA ofEscherichia coli K-12. NANCY SCHAUS, KATHY O'DAY,WENDY PETERS, AND ANDREW WRIGHT* .................. . 904-913

Transductional Mapping of ksgB and a New Tn5-Induced Kasuga-mycin Resistance Gene, ksgD, in Escherichia coli K-12. KENE. FOUTS AND STEPHEN D. BARBOUR* ..................... 914-919

Identification and Cloning of the Genetic Determinant That Encodesfor the K88ac Adherence Antigen. PATRICIA L. SHIPLEY,* GOR-DON DOUGAN, AND STANLEY FALKOW ...................... 920-925

Purification of Polypeptide P6 with a Molecular Weight of 6,000 fromthe Vegetative Chromosomes of Bacillus subtilis. TATSUO NA-KAYAMA,* YUMIKO KUROGI, AND MAKI IRIKURA ............. 953-957

Replication of M13 oriC Bacteriophages in an Escherichia coli repMutant Is Dependent on the Cloned Escherichia coli Replica-tion Origin. LAURIE SIMON KAGUNI, JON M. KAGUNI, ANDDAN S. RAY* ............................................ 974-979

Transfornation of Bacillus thuringiensis Protoplasts by PlasmidDeoxyribonucleic Acid. PHYLLIS A. W. MARTIN,* JOHN R.LOHR, AND DONALD H. DEAN ............................. 980-983

Organization and Regulation of the ilvGEDA Operon in Salmonellatyphimurium LT2. CLAIRE M. BERG* AND KAREN J. SHAW 984-989

CONTENTS

Genetic Regulation of Variable Vi Antigen Expression in a Strain ofCitrobacter freundii. NORMA J. SNELLINGS,* E. M. JOHNSON,DENNIS J. KOPECKO, HUGH H. COLLINS, AND L. S. BARON ... 1010-1017

Characterization and Mapping of Temperature-Sensitive DivisionInitiation Mutations of Bacillus subtilis. HEATHER CALLISTERAND R. G. WAKE* .............. .......................... 1042-1051

Specialized Transducing Bacteriophage Lambda Carrying the Struc-tural Gene for a Major Outer Membrane Matrix Protein ofEscherichia coli K-12. NORIHIRO MUTOH, TADAKO NAGA-SAWA, AND SHOJI MIZUSHIMA* .......... ................... 1085-1090

Selection for Loss of Tetracycline Resistance by Escherichiacoli. STANLEY R. MALOY* AND WILLIAM D. NUNN ......... 1110-1112

Host Requirements for Growth of a A-P22 Hybrid in Escherichiacoli. KENT D. TAYLOR AND HIROAKI SHIZUYA* ..... ....... 1113-1115

Temperature Sensitivity of the Regulation of Nitrogenase Synthesisby Klebsiella pneumoniae. JIABI ZHU AND WINSTON J.BRILL* .......................... 1116-1118

Plasmids and TransposonsProperties of Six Pesticide Degradation Plasmids Isolated from Al-

caligenes paradoxus and Alcaligenes eutrophus. R. H. DONAND J. M. PEMBERTON* .................................... 681-686

Fornation ofEscherichia coli Hfr Strains by Integrative Suppressionwith P Group Plasmid RP1. RONALD R. MARTIN, CYDNEY L.THORLTON, AND LEON UNGER* ............................ 713-721

Transposition of the Oxacillin-Hydrolyzing Penicillinase Gene.ToMOKO YAMAMOTO,* MICHIYASu TANAKA, CHIEKO NOHARA,YUKIKO FUKUNAGA, AND SABURO YAMAGISHI ..... .......... 808-813

sfrA and sfrB Products of Escherichia coli K-12 Are TranscriptionalControl Factors. LOTHAR BEUTIN, PAUL A. MANNING, MARKACHTMAN,* AND NEIL WILLETTS ........................... 840-844

Physical Characterization of Bacteroides fragilis R PlasmidpBF4. RODNEY A. WELCH AND FRANCIS L. MACRINA* ...... 867-872

Plasmid Transfer Within and Between Serologically Distinct Strainsof Rhizobium japonicum, Using Antibiotic Resistance Mutantsand Auxotrophs. W. P. PILACINSKI AND E. L. SCHMIDT* .... 1025-1030

Plasmid Transformation in Haemophilus influenzae. WILLIAM L.ALBRITTON,* JOHN W. BENDLER, AND JANE K. SETLOW ...... 1099-1101

Conjugative R Plasmids in Group C and G Streptococci. LYDIEBOUGUELERET, GILDA BIETH, AND THEA HORODNICEANU ... 1102-1105

Eucaryotic CellsEffect of the gln-lb Mutation on Nitrogen Metabolite Repression in

Neurospora crassa. NIGEL S. DUNN-COLEMAN AND REGINALDH. GARRETT* ............................................ 884-888

Natural Heterozygosity in Candida albicans. WILLIAM L. WHELANAND PAUL T. MAGEE* ......................... 896-903

MembranesStructural Comparison of Neisseria gonorrhoeae Outer Membrane

Proteins. JOHN E. HECKELS .............................. 736-742Lactoperoxidase-'25I Localization of Salt-Extractable Alkaline Phos-

phatase on the Cytoplasmic Membrane of Bacillus lichenifor-mis. DONALD B. SPENCER AND F. MARION HULErr* 934-945

CONTENTS

Neisseria gonorrhoeae Cell Envelope: Permeability to HydrophobicMolecules. PAUL G. LYSKO AND STEPHEN A. MORSE* ....... 946-952

Identification and Preliminary Characterization of Vibrio choleraeOuter Membrane Proteins. JENNY T. KELLEY AND CHAR-LOTTE D. PARKER* ............... ........................ 1018-1024

Genetic Dissection of Catalytic Activities of the Salmonella typhi-murium Mannitol Enzyme II. JOHN E. LEONARD AND MILTONH. SAIER, JR.* ........................................... 1106-1109

Physiology and MetaboismCharacterization and Properties of an LL-Oligopeptidase from Spor-

ulating Cells of Bacillus sphaericus. MARIE-JEANNE VACH-ERON, MICHELINE GUINAND, AND GEORGES MICHEL* ........ 675-680

Synthesis and Metabolism of Uracil-Containing DeoxyribonucleicAcid in Escherichia coli. HUBER R. WARNER,* BRUCE K.DUNCAN, CHARLES GARRETT, AND JAN NEUHARD .... ....... 687-695

Molybdenum Accumulation and Storage in Klebsiella pneumoniaeand Azotobacter vinelandii. PHILIP T. PIENKOS AND WINSTONJ. BRILL* ................................................ 743-751

Isolation and Properties of a Bacillus subtilis Mutant Unable toProduce Fructose-Bisphosphatase. YASUTARO FUJITA ANDERNST FREESE* .................. ...... 760-767

Effect of Glutamine on Enzymes of Nitrogen Metabolism in Bacillussubtilis. KATHRYN L. DESHPANDE, JON R. KATZE, AND JAMESF. KANE* ................................................ 768-774

Regulation of Derepressed Synthesis of Arylsulfatase by TyramneOxidase in Salmonella typhimurium. YOSHIKATSU MUROOKA*AND TOKUYA HARADA ............ ........................ 796-802

Effect of Arsenate on Chemotactic Behavior of Escherichiacoli. TAKAO ARAI ................. ....................... 803-807

Inhibition of Nitrogenase' Activity by NH' in Rhodospirillum rub-rum. WILLIAM J. SWEET AND ROBERT H. BURRIS* ......... 824-831

Regulation Of D-Alamine Carboxypeptidase and Peptidoglycan Cross-Linkage in Amino Acid-Deprived Escherichia coli. ROBIN E.HARKNESS, DAVID MIRELMAN, AND EDWARD E. ISHIGURO* ... 845-849

Lipid Metabolism During Encystment ofAzotobacter vinelandii. R.N. REUSCH AND H. L. SADOFF* ........ .................... 889-895

Effect of Cobalt on Synthesis and Activation of Bacillus lichenifor-mis Alkaline Phosphatase. DONALD B. SPENCER, CHAI-PAOCHEN, AND F. MARION HULETT* ....... .................... 926-933

In Vivo and In Vitro Chemotactic Methylation in Bacillussubtilis. ABUL H. J. ULLAH AND GEORGE W. ORDAL* ....... 958-965

Comparative Bioenergetics of Sulfate Reduction in Desulfovibrioand Desulfotomaculum spp. CHI-LI LIU AND H. D. PECK,JR.* ...................................... ............... 966-973

Aerotaxis in Salmonella typhimurium: Role of Electron Transport.DANIEL J. LASZLO AND BARRY L. TAYLOR* ..... ............ 990-1001

Pyridine Nucleotide Cycle of Salmonella typhimurium: In VitroDemonstration of Nicotinamide Adenine Dinucleotide Glyco-hydrolase, Nicotinamide Mononucleotide Glycohydrolase, andNicotinamide Adenine Dinucleotide Pyrophosphatase Activi-ties. JOHN W. FOSTER.1002-1009

CONTENTS

Cysteine and Growth Inhibition of Escherichia coli: Threonine De-aninase as the Target Enzyme. CHARLES L. HARRIS ........ 1031-1035

Comparative Biochemistry of Entomocidal Parasporal Crystals ofSelected Bacillus thuringiensis Strains. DANA J. TYRELL, LEEA. BULLA, JR.,* ROBERT E. ANDREWS, JR., KARL J. KRAMER,LOREN I. DAVIDSON, AND PHILIP NORDIN .............. ..... 1052-1062

Effect of a New Pyrimidine Analog on Bacillus subtilis Growth. G.RAUGEI, M. BAzzIcALuPO, G. FEDERICI, E. GALLORI, R. PEPINO,AND M. POLSINELLI* ..................................... 1079-1081

Escherichia coli Growth Studied by Dual-Parameter Flow Cytopho-tometry. HARALD B. STEEN* AND ERIK BOYE.1091-1094

A Salmonella typhimurium Mutant Dependent upon Carbamyl As-partate for Resistance to 5-Fluorouracil Is Specifically Affectedin Ubiquinone Biosynthesis. VICTOR L. ZAK AND ROD A.KELLN* ................................................. 1095-1098

EnzymologyIdentification of a Lysin Associated with a Bacteriophage (A25)

Virulent for Group A Streptococci. JOHN E. HILL* AND LEWISW. WANNAMAKER.696-703

Purification and Properties of Endo-a-1,3-Glucanase from a Strep-tomyces chartreusis Strain. TADAMICHI TAKEHARA, MASA-KAZU INOUE,* ToSHIO MORIOKA, AND KANAE YOKOGAWA .... 729-735

Comparative Allostery of3-Deoxy-D-arabino-Heptulosonate 7-Phos-phate Synthetase as an Indicator of Taxonomic Relatedness inPseudomonad Genera. ROBERT J. WHITAKER, GRAHAM S.BYNG, ROBERT L. GHERNA, AND Roy A. JENSEN* .752-759

D-Fructose Dehydrogenase of Gluconobacter industrius: Purifica-tion, Characterization, and Application to Enzymatic Microde-termination of D-Fructose. MINORU AMEYAMA,* EMIKO SHIN-AGAWA, KAZUNOBU MATSUSHITA, AND OSAo ADACHI. 814-823

Characterization of a Site-Specific Restriction Endonuclease fromStreptomyces aureofaciens. JOZEF TIMKO, ARNOLD H. HOR-WITZ, JAN ZELINKA, AND GARY WLCOX...................x.873-877

ErratumEvidence for Isofunctional Enzymes Used in m-Cresol and 2,5-Xy-

lenol Degradation via the Gentisate Pathway in Pseudomonasalcaligenes. CHIT LAA POH AND RONALD C. BAYLY . 1119

Volume 145 Contents for March 1981 Number3Morphology and Ultrastructure

Nature of the Carbohydrate and Phosphate Associated with ColB2and EDP208 Pilin. G. D. ARMSTRONG, L. S. FROST, H. J.VOGEL, AND W. PARANCHYCH*.1167-1176

tJltrastructure of the Septal Complex in Hyphae of Cryptococcuslaurentii. JUDITH C. RHODES,* KYUNG J. KWON-CHUNG, ANDTERRY J. POPKIN ............. ................... 1410-1412

Genetics and Molecular BiologyMolecular Cloning of TOL Genes xylB and xylE in Escherichia

coli. SACHIYE INOUYE, ATSUSHI NAKAZAWA, AND TERUKONAKAZAWA* ............................................. 1137-1143

CONTENTS

Assimilation of Single-Stranded Donor Deoxyribonucleic Acid Frag-ments by Nucleoids of Competent Cultures of Bacillus sub-tilis. JAN VAN RANDEN* AND GERARD VENEMA .... ........ 1177-1188

Effect of Ethylenediaminetetraacetic Acid on Deoxyribonucleic AcidEntry and Recombination in Transformation of a Wild-TypeStrain and a rec-1 Mutant of Haemophilus influenzae.MATTHIEU NOTEBORN, GERARD VENEMA, AND JAN KooISTRA* 1189-1195

Growth Rate-Dependent Control of Chromosome Replication Initi-ation in Escherichia coli. GORDON CHURCHWARD, EVANGE-LINE ESTIVA, AND HANS BREMER* ......................... 1232-1238

Genetic and Physiological Characterization of a Spontaneous Mutantof Escherichia coli B/r with Aberrant Control of Deoxyribonu-cleic Acid Replication. KYUNG-KOO CHOUNG, EVANGELINEESTIVA, AND HANS BREMER* ...................... .. 1239-1248

Genetic and Physiological Tests of Three Phosphate-Specific Trans-port Mutants of Escherichia coli. GERALD ZUCKIER AND AN-NAMARIA TORRIANI* ...................................... 1249-1256

Reexamination of the Genome Size of Myxobacteria, Including theUse of a New Method for Genome Size Analysis. THOMAS YEEAND MASAYORI INOUYE* ................. .......... 1257-1265

Analysis of Bacillus subtilis Sporulation with Spore-Converting Bac-teriophage PMB12. DENNIS M. KINNEY AND MICHAEL G.BRAMUCCI* .............................................. 1281-1285

Rifampin Resistance Mutations That Alter the Efficiency of Tran-scription Termination at the Tryptophan Operon Attenuator.CHARLES YANOFSKY* AND VIRGINIA HoRN ................. 1334-1341

Deoxyribonucleic Acid Replication in Permeable and Fully ViableEscherichia coli Cells. ERIK BOYE,* STEIN ALVER, AND KIR-STEN SKARSTAD .......................................... 1413-1416

Complementation Tests Between Alkaline Phosphatase-ConstitutiveMutants (phoS andphoT) of Escherichia coli. RUTH LEVITZ,RAYA BITTAN, AND EZRA YAGIL* ...... .................... 1432-1435

Inhibition of Deoxyribonucleic Acid Replication in Bacillus brevisby Ribonucleic Acid Polymerase Inhibitors. SUDHA BHATTA-CHARYA AND NILIMA SARKAR* ....................... 1442-1444

Regulation of Ribosomal Protein Synthesis in an Escherichia coliMutant Missing Ribosomal Protein Ll. SUE JINKS-ROBERT-SON AND MASAYASU NOMURA* ............................ 1445-1447

Versatile Cloning Vector for Pseudomonas aeruginosa. DAVID 0.WOOD,* M. FLYNN HOLLINGER, AND MICHAEL B. TINDOL .... 1448-1451

Plasmids and TransposonsLarge Plasmids of Fast-Growing Rhizobia: Homology Studies and

Location of Structural Nitrogen Fixation (nif) Genes.RAMESWARAM K. PRAKASH, ROB A. SCHILPEROORT,* AND

MARCO P. NUTI ........................................ 1129-1136Homology Between Escherichia coli Plasmids ColEl and p15A.

ROBERT E. BIRD ............... .......................... 1305-1309Functional Organization of Plasmid pKM1Ol. PAMELA J. LANGER,

WILLIAM G. SHANABRUCH, AND GRAHAM C. WALKER* ....... 1310-1316

Rapid Procedure for Detection and Isolation of Large and SmallPlasmids. C. I. KADO* AND S.-T. LIU .................... 1365-1373

CONTENTS

Tetracycline Resistance Genes from Bacillus Plasmid pAB124 Con-fer Decreased Accumulation ofthe Antibiotic in Bacillus subtilisbut Not in Escherichia coli. SAuRH ECCLES, ANDREW DOCH-ERTY, IAN CHOPRA,* STUART SHALES, AND PETER BALL ...... 1417-1420

Intrastrand Base Pairing in Single-Stranded Deoxyribonucleic Acidfrom ColEl-Derived Plasmid pCR1. THOMAS D. EDLIND ANDGARRET M. IHLER* ............. ....... ... .. ... .......... 1436-1441

Eucaryotic CellsTurnover of Ammonium-Inducible Glutamate Dehydrogenase Dur-

ing Induction and Its Rapid Inactivation After Removal ofInducer from Chlorella sorokiniana Cells. NEwELL F. BAS-COMB, ANTHONY T. YEUNG, KATHERINE J. TURNER, AND ROB-ERT R. SCHMIDT* .......... ..................... ... 1266-1272

Corresponding Changes in Kynurenine Hydroxylase Activity, Mem-brane Fluidity, and Sterol Composition in Saccharomyces cere-visiae Mitochondria. -COLLEEN A. MCLEAN-BOWEN AND LEOW. PARKS* .............................................. 1325-1333

Timing of Ribosome Synthesis During Ascosporogenesis of YeastCells: Evidence for Early Function of Haploid Daughter Ge-nomes. JANET R. EMANUEL AND P. T. MAGEE* ... 1342-1350

Subcellular Compartmentation in Control of Converging Pathwaysfor Proline and Arginine Metabolism in Saccharomyces cerevi-siae. MARJORIE C. BRANDRISS* AND BORIS MAGASANIK .... 1359-1364

Curing of the 21, DNA Plasmid from Saccharomyces cerevisiae.AKIo TOH-E* AND REED B. WICKNER ...................... 1421-1424

Regulation of Dimorphism in Histoplasma capsulatum by CyclicAdenosine 3',5'-Monophosphate. JUDITH MEDOFF, ERIC JA-COBSON, AND GERALD MEDOFF* .......................... 1452-1455

MembranesComparative Studies of Two Membrane Fractions Isolated from

Chemotrophically and Phototrophically Grown Cells of Rhodo-pseudomonas capsulata. AUGUSTO F. GARCIA, GERHARTDREWS,* AND HORST H. REIDL ................... ......... 1121-1128

In Vivo Intermembrane Transfer of Phospholipids in the Photosyn-thetic Bacterium Rhodopseudomonas sphaeroides. BRIAN D.CAIN, CAROLYN D. DEAL, ROBERT T. FRALEY, AND SAMUELKAPLAN* ... ...................1154-1166

Topographical Distribution of Penicillin-Binding Proteins in theEscherichia coli Membrane. CHRISTINE E. BUCHANAN 1293-1298

Proton Motive Force Across the Membrane ofMycoplasma gallisep-ticum and Its Possible Role in Cell Volume Regulation. SHLOMOROTTEM, CAREY LINKER, AND T. HASTINGS WILSON* .1299-1304

Genetic and Biochemical Characterization of Periplasmic-Leaky Mu-tants of Escherichia coli. JEAN-CLAUDE LAZZARONI* ANDRAYMOND C. PORTALIER ....................... 1351-1358

Citrate-Tris(hydroxymethyl)aminomethane-Mediated Release ofOuter Membrane Sections from the Cell Envelope of a Deep-Rough (Heptose-Deficient Lipopolysaccharide) Strain of Esch-erichia coli 08. R. T. IRVIN,* T. J. MACALISTER, R. CHAN,AND J. W. COSTERTON .................................... 1386-1396

Tris(hydroxymethyl)aminomethane Buffer Modification of Esche-richia coli Outer Membrane. R. T. IRVIN,* T. J. MACALISTER,AND J. W. COSTERTON ........................ 1397-1403

CONTENTS

Phospholipid Biosynthesis Is Required for Stalk Elongation in Cau-lobacter crescentus. JAMES D. MANSOUR, SUSAN HENRY, ANDLUCILLE SHAPIRO* ....... .......... ..... ..... ... . 1404-1409

Physiology and MetabolismNickel Requirement for Active Hydrogenase Formation in Alcali-

genes eutrophus. BARBEL FRIEDRICH,* ELKE HEINE, AN-NETTE FINCK, AND CORNELIUS G. FRIEDRICH.... 1144-1149

Threonine as a Carbon Source for Escherichia coli. T. T. K. CHANAND E. B. NEWMAN* ................................ .. 1150-1153

Change in Intracellular pH of Escherichia coli Mediates the Chem-otactic Response to Certain Attractants and Repellents. DAVIDR. RAPASKE AND JULIUS ADLER* ................. . 1196-1208

Cytoplasmic pH Mediates pH Tais and Weak-Acid Repellent Taxisof Bacteria. MAY KIHARA AND ROBERT M. MACNAB*. 1209-1221

Attachment of the Main Chain to the Linkage Unit in Biosynthesisof Teichoic Acid. HAMISH A. I. MCARTHUR, IAN C. HANCOCK,AND JAMES BADDILEY*.1222-1231

Cyclic Adenosine 3',5'-Monophosphate Levels in Pseudomonas pu-tida and Pseudomonas aeruginosa During Induction and Car-bon Catabolite Repression of Histidase Synthesis. ALLEN T.PHILLIPS* AND LORRAINE M. MULFINGER.1286-1292

Two Succinic Semialdehyde Dehydrogenases Are Induced WhenEscherichia coli K-12 Is Grown on y-Aminobutyrate. MARK I.DONNELLY AND RONALD A. COOPER* ................... ... 1425-1427

Repair of Thermal Damage to the Escherichia coli Nucleoid. JOSER. PELLON AND REINALDO F. GOMEZ* ................... ... 1456-1458

Properties of the Lactose Transport System in Klebsiella sp. StrainCT-1. Ko IMAI AND BmARY G. HALL*.1459-1462

Conditions That Trigger Guanosine Tetraphosphate Accumulationin Caulobacter crescentus. TERESA A. CHIAVEROTTI, GARYPARKER, JONATHAN GALLANT, AND NINA AGABIAN* ........ . 1463-1465

EnzymologyPurification and Characterization of Cytidine 5'-Triphosphate:Cyti-

dine Monophosphate-3-Deoxy-D-manno-Octulosonate Cytidyl-yltransferase. PAUL H. RAY,* CHARLES D. BENEDICT, ANDHANS GRASMUK ................................. 1273-1280

Resolution of Distinct Selenium-Containing Formate Dehydrogen-ases from Escherichia coli. JOHN C. Cox, ELLEN S. EDWARDS,AND JOHN A. DEMOSS*.1317-1324

Adenylate Energy Charge in Escherichia coli CR341T28 and Prop-erties of a Heat-Sensitive Adenylate Kinase. CHRISTOPHER C.GLEMBOTSKI, ASTRID G. CHAPMAN, AND DANIEL E.ATKINSON* .......................... 1374-1385

Arylsulfatase from Pseudomonas sp. Strain C12B: Purification toHomogeneity, Immunological Analysis, and Physical Proper-ties. JAMES R. GEORGE AND JOHN W. FITZGERALD* ........ 1428-1431

Month Date of Issue Pages

January 22 January 1981 1-673February 25 February 1981 675-1119March 25 March 1981 1121-1465