Journal of Clinical PathologyJanuary 1985 Vol 38 No 1ContentsReview articleFine needle aspiration cytology JV LEVER, PA TROTT, AJ WEBB page 1A monoclonal antibody that detects HLA-D region antigen in routinely fixed, wax embedded sections ofnormal and neoplastic lymphoid tissues AA EPENETOS, LG BOBROW, TE ADAMS, CM CCILLINS, PG ISAACSON,WF BODMER page 12An immunoperoxidase study of epithelial marker antigens in ulcerative colitis with dysplasia andcarcinoma DC ALLEN, JD BIGGART, JC ORCHIN, HEATHER FOSTER page 18Large bowel mucosal dysplasia and carcinoma in ulcerative colitis DC ALLEN, JD BIGGART, PC PYPERpage 30Morphometric assessment of reflux oesophagitis in fibreoptic biopsy specimens LR JARVIS, J DENT,R WHITEHEAD page 44Argentaffin and argyrophil reactions and serotonin content of endocrine tumours CA WELLS, SM TAYLOR,AC CUELLO page 49Normal values for the different classes of venous blood mononuclear cells defined by monoclonalantibodies LK GOFF, JA HABESHAW, ML ROSE, JA GRACIE, W GREGORY page 54Diminished creatinine clearance in anorexia nervosa: reversal with weight gain F BOAG, J WEERAKOON,J GINSBURG, CWH HAVARD, P DANDONA page 60Plasma fibronectin and thyroid function W GRANINGER, K PIRICH, K DERFLER, W WALDHAUSL page 6Sideroblastic colonies in erythroid cultures grown from normal human marrow S KAABA, A JACOBS,K BARNES page 68SS-A (Ro) antibody in random mother-infant pairs M CALMES, BA BARTHOLOMEW page 73Cryptosporidiosis in immunocompetent children D ISAACS, GH HUNT, AD PHILLIPS, EH PRICE, F RAAFAT,JA WALKER-SMITH page 76Gas chromatographic identification of Clostridium difficile and detection of cytotoxin from a modifiedselective medium PN LEVETr, KD PHILLIPS page 82Killing of Escherichia coli by human polymorphonuclear leucocytes in the presence of Bacteroidesfragilis WAC VEL, F NAMAVAR, AMJJ VERWEIJ-van VUGHT, ANB PUBBEN, DM MacLAREN page 86Clinical evaluation of a fluorescent antibody test for the serological diagnosis of streptococcalendocarditis DC SHANSON, NICOLA KIRK, R HUMPHREY page 92Study of precipitation reactions to Actinomyces israelii antigens in uterine secretions ELISABETH PERSSON,K HOLMBERG page 99Serodiagnosis of ocular toxocariasis: a comparison of two antigens LT GLICKMAN, RB GRIEVE, SS LAURIA,DEBRA L JONES page 103Technical methodsImproved gas-liquid chromatography method for the identification of Clostridium difficile JD BERG,RG MILLS, DJ COLEMAN page 108Demonstration of Mycobacterium tuberculosis in epoxy resin sections by the Ziehl-Neelsentechnique KE BRASSIL page 1 10Micromodified cytomegalovirus antibody screening test SM HENRY, AM RAMIREZ, DG WOODFIELDpage 111Letters to the Editor page 1 13Book review page 1 16Noticepage 116Some new titles page 1 16

ASTM CODEN: JCPAAK 38(1) 1-116 (1985) ISSN 0021-9746

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Fine needle aspiration cytology Jv LEVER, PA TROTT, AJ WEBB........................................................... 1A monoclonal antibody that detects HLA-D region antigen in routinely fixed, wax embedded sections of normal and neoplasticlymphoid tissues AA EPENETOS, LG BORROW, TE ADAMS, CM COLLINS, PG ISAACSON, WF BODMER ............................... 12An immunoperoxidase study of epithelial marker antigens in ulcerative colitis with dysplasia and carcinoma DC ALLEN, JD BIGGART,JC ORCHIN, HEATHER FOSTER..........................................................................................18Large bowel mucosal dysplasia and carcinoma in ulcerative colitis DC ALLEN, JD BIGGART, PC PYPER ..... .................... 30Morphometric assessment of reflux oesophagitis in fibreoptic biopsy specimens -LR JARVIS, J DENT, R WHITEHEAD............... 44Argentaffin and argyrophil reactions and serotonin content of endocrine tumours CA WELLS, SM TAYLOR, AC CUELLO ........... 49Normal values for the different classes of venous blood mononuclear cells defined by monoclonal antibodies LK GOFF, JA HABESHAW,ML ROSE, JA GRACIE, W GREGORY..................................................................................... 54Diminished creatinine clearance in anorexia nervosa: reversal with weight gain F BOAG, J WEERAKOON, J GINSBURG, CWH HAVARD,P DANDONA ....................................................................................................... 60Plasma fibronectin and thyroid function W GRANINGER, K, PIRICH, K DERFLER, W WALDHAUSL ............................... 64Sideroblastic colonies in erythroid cultures grown from normal human marrow S KAABA, A JACOBS, K BARNES ..... ............ 68SS-A (Ro) antibody in random mother-infant pairs M CALMES, BA BARTHOLOMEW ......................................... 73Cryptosporidiosis in immunocompetent children D ISAACS, GH HUNT, AD PHILLIPS, EH PRICE, F RAAFAT, JA WALKER-SMITH........ 76Gas chromatographic identification of Clostridiwn difficile and detection of cytotoxin from a modified selective medium PN LEVETT,KD PHILLIPS ...................................................................................................... 82Killing of Escherichia coli by human polymorphonuclear leucocytes in the presence of Bacteroides fragilis WAC VEL, F NAMAVAR,AMJJVERWEIJ-van VUGHT, ANB PUBBEN, DM MaCLAREN ................................................................... 86Clinical evaluation ofa fluorescent antibody test for the serological diagnosis of streptococcal endocarditis DC SHANSON, NICOLA KIRK,R HUMPHREY...................................................................................................... 92Study of precipitation reactions to Actinomyces israelii antigens in uterine secretions ELISABETH PERSSON, K HOLMBERG.......... 99Serodiagnosis of ocular toxocariasis: a comparison of two antigens LT GLICKMAN, RB GRIEVE, SS LAURIA, DEBRA L JONES ........ 103Technical methodsImproved gas-liquid chromatography method for the identification of Clostridium difficile 3D BERG, RG MILLS, DJ COLEMAN 108Demonstration of Mycobacterium tuberculosis in epoxy resin sections by the Ziehl-Neelsen technique KE BRASSIL .... .......... 110Micromodified cytomegalovirus antibody screening test SM HENRY, AM RAMIREZ, DG WOODFIELD ............................. IllLetters to the Editor............................................................................................... 113Book review............................................................................................... 116Notice............................................................................................... 116Some new titles ............................................................................................... 116

No 2 FEBRUARY 1985

Review articleBiochemical enzyme analysis in acute leukaemia HG DREXLER, G GAEDICKE, J MINOWADA ............................... . .... 117Kinetics, distribution, and sites of destruction of indium-I 11 oxine labelled red cells in haemolytic anaemia AduP HEYNS, MG LOTTER,HF KOTZE, P WESSELS, H PIETERS, PN BADENHORST ....................................................................... 128lCSH/ICTH recommendations for reporting prothrombin time in oral anticoagulant control INTERNATIONAL COMMITTEE FORSTANDARDIZATION IN HAEMATOLOGY AND INTERNATIONAL COMMITTEE ON THROMBOSIS AND HAEMOSTASIS ......................... 133Erythrocyte deformability in peripheral occlusive arterial disease D BAREFORD, GS LUCAS, NM CALDWELL, PCW STONE, S BAAR, J STUART 135Immunohistochemical localisation of tissue plasminogen activator and urokinase in the vessel wall A LARSSON, B ASTEDT........ 140Heart transplant pathology: the British experience ARIELA POMERANCE, PGI STOVIN......................................... 146Giant cell myocarditis: evidence for the macrophage origin of the giant cells JM THEAKER, KC GATTER, A HERYET, DJ EVANS, J O'D MCGEE 160Immunohistological diagnosis of central nervous system tumours using a monoclonal antibody panel HB COAKHAM, JA GARSON,PATRICIA M ALLAN, EDNA I HARPER, BETTY BROWNELL, JT KEMSHEAD, E BIRGITTE LANE ......................................... 165Assessment of dysplasia in colorectal adenomas; an observer variation and morphometric study LJR BROWN, NC SMEETON, MF DIXON 174Immunocytochemical reaction of Cal and HMFG2 monoclonal antibodies with cells from serous effusions S SINGER, MMBODDINGTON, EA HUDSON ........................................................................................... 180Liver aspiration in the diagnosis of hepatocellular carcinoma in the Gambia A AJDUKIEWICZ, A CROWDEN, ELIZABETH HUDSON ... 185Serial study of C reactive protein concentrations in cardiac allograft recipients GD HARKISS................................. 193Study of discrepancies in rubella haemagglutinin titrations and a reappraisal of diluents used in the rubella haemagglutinationinhibition technique HGS MURRAY, JUDITH STANTON, PS GARDNER ...................................................... 198Comparison of five different methods of rubella IgM antibody testing HEATHER CUBIE, ELIZABETH EDMOND.................... 203A study of workload units in five microbiology laboratories RY CARTWRIGHT, JOAN R DAVIES, C DULAKE, RJC HART, CA MORRIS, PJWILKINSON ..................................................................................................... 208Microcomputer system for multistep specimen processing and reporting in a microbiology laboratory R ASHLEY, B DOWNING 215Development and application of an enzyme linked immunosorbent assay for Clostridium perfringens type A enterotoxin BARBARAA BARTHOLOMEW, MF SRRINGER, GN WATSON, RJ GILBERT............................................................... 222Leucocyte esterase determination as a secondary procedure for urine screening PM LEIGHTON, JA LIrrLE ..... ................. 229

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PageTechnical methodEffect of antibiotic concentration in a selective medium on the isolation of Clostridium dificile from faecal specimens PN LEVETT 233Letters to the Editor............................................................................................... 235Book reviews ................................................................................................. 238Notices ................................................................................................. 240

No 3 MARCH 1985

Review articleThe pathobiology of the osteoclast TJ CHAMBERS...................................................................... 241Glomerular thrombi in renal allografts associated with cyclosporin treatment GH NEILD, ROWENA REUBEN, RB HARTLEY, JS CAMERON 253Incidence of intraglomerular platelets in steroid sensitive nephrotic syndrome JMC UTTLEY, RA ELTON, CM HILL, MK MACDONALD.. 259Functional and structural changes of the human proximal small intestine after cytotoxic therapy D CUNNINGHAM, RJ MORGAN, PRMILLS, LM NELSON, PG TONER, M SOUKOP, CS MCARDLE, RI RUSSELL ......................................................... 265The Cronkhite-Canada syndrome: an ultrastructural study of pathogenesis D JENKINS, PM STEPHENSON, BB SCOTT .... .......... 271Mucin producing microfollicular adenoma of the thyroid C RIGAUD, F PELTIER, WV BOGOMOLETZ ............................ 277Morphometric studies of age related changes in normal human breast and their significance for evolution of mammary cancer swHUTSON, PN COWEN, CC BIRD.......................... 281Subcutaneous phaeohyphomycosis: a histopathological study of nine cases from Malawi PJ O'DONNELL, MSR HUTT .... ......... 288Gastro-oesophageal candidiasis: diagnosis by brush cytology JENNIFER A YOUNG, E ELIAS ................................... 293Refractory anaemia terminating in a combined lymphoproliferative and myeloproliferative disorder L CAMBA, MV JOYNER....... 297Granulocyte and monocyte surface membrane markers in the myelodysplastic syndromes RE CLARK, TG HOY, A JACOBS......... 301Evaluation of the common conditions associated with eosinophilia CG TEO, M SINGH, WC TING, LC HO, YW ONG, LC SEET .... .... 305Postsplenectomy sepsis 10 years or more after operation DIK EVANS ..................................................... 309Acute phase response of serum amyloid A protein and C reactive protein to the common cold and influenza JT WHICHER, RECHAMBERS, J HIGGINSON, L NASHEF, PG HIGGINS......................................................................... 312Enhanced luminescence enzyme immunoassay for factor VIII related antigen HX WANG, J GEORGE, GHG THORPE, RA STOTT, LJ KRICKA,TP WHITEHEAD .................................................................................................... 317Blood group antigens A, B, H, Le', Leb, and I(Ma) in resting and tetragastrin stimulated gastric juice of patients with non-neoplasticdiseases of the stomach JEAN K PICARD, KYOKO HOTTA, TEN FEIZI ........................................................ 320Familial abnormalities of thyroxine binding proteins: some problems of recognition and interpretation JULIA E NEILD, PGH BYFIELD,MRA LALLOZ, DIANA TAIT, JH MARIGOLD, DN CROFT, BRENDA M SLAVIN ..................................... 327Simplified restriction endonuclease method for typing and subtyping adenoviruses JM DARVILLE ............................. 331Effects of media, working practice, and automation on the rapid detection of bacteraemia JE CORKILL ..... .................. 336Assessment of rapid methods of pneumococcal antigen detection in routine sputum bacteriology M WHITBY, KG KRISTINSSON, M BROWN 341Comparison of enzyme linked immunosorbent assay and enzyme linked fluorescence immunoassay for detection of antibodies againstChlamydia trachomatis K NUMAZAKI, S CHIBA, T MOROBOSHI, T KUDOH, T YAMANAKA, T NAKAO............................... 345Incidence of anti-intermediate filament antibody in serum samples of students with suspected glandular fever PK KATAAHA, EJHOLBOROW, JMB EDWARDS .......................................................................................... 351Letters to the Editor............................................................................................... 355Book reviews ................................................................................................. 356Notice................................................................................................. 360

No 4 APRIL 1985

Bronchopulmonary disease in coeliac patients C EDWARDS, A WILLIAMS, P ASQUITH......................................... 361Jejunal mucosal morphology in healthy north Indian subjects MK BENNETT, GK SACHDEV, DP JEWELL, BS ANAND................ 368Immunocytochemistry in the diagnosis of Whipple's disease DJ EVANS, MH ALI ............................................ 372Histochemical demonstration of chymotrypsin like serine esterases in mucosal mast cells in four species including man JF HUNTLEY,GFJ NEWLANDS, S GIBSON, ANNE FERGUSON, HRP MILLER .................................................................. 375Mucosal abnormalities at the anastomosis site in patients who have had intestinal resection for colonic cancer JP SUNTER, MJ HIGGS,WK COWAN........................................................................................................ 385Antral hypertrophic gastritis: a rare cause of iron deficiency GWH STAMP, K PALMER, nJ MISIEWICZ ..... ...................... 390Widespread presence of cytoplasmic HBcAg in hepatitis B infected liver detected by improved immunochemical methods EJ GOWANS,CJ BURRELL............................................................................. ...... I------ ....

393Investigation into the value of Papanicolaou stained cervical smears for the diagnosis of chlamydial cervical infection GE FORSTER,I COOKEY, FE MUNDAY, PI RICHMAN, R JHA, D COLEMAN, E THOMAS, DA HAWKINS, RT EVANS, D TAYLOR-ROBINSON .... ............ 399An assessment of the value of electron microscopy in tumour diagnosis C FISHER, AD RAMSAY, M GRIFFITHS, J MCDOUGALL ....... 403Effect of neuraminidase on the expression of the 3-fucosyl-N-acetyllactosamine antigen in human tissues AJ HOWlE, G BROWN... 409Histogenesis of haemangioblastomas: an immunocytochemical and ultrastructural study in a case ofvon Hippel-Lindau syndrome SMISMAIL, B JASANI, GILLIAN COLE ...................................................................................... 417A practical heparin reduction algorithm: execution and operational characteristics HE BRANSON, D van STRALEN, JOANNE SCHOTTINGER,W PETERS, Y ENDO .................................................................................................. 422A bleeding disorder due to deficiency of a2-antiplasmin P KETTLE, ELIZABETH E MAYNE ..................................... 428Effect of anticoagulants on the chromogenic Limulus lysate assay for endotoxin Is MCCONNELL, J COHEN ...... ............... 430Studies on the procurement of blood coagulation factor VIII: effects of plasma freezing rate and storage conditions on cryoprecipitatequality A FARRUGIA, C PROWSE.433

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Verotoxin and neuraminidase induced platelet aggregating activity in plasma: their possible role in the pathogenesis ot the haemolyticuraemic syndrome PE ROSE, JA ARMOUR, CE WILLIAMS, FGH HILL ......................................................... 438Uptake of ciprofloxacin by macrophages CSF EASMON, JP CRANE ........................................................ 442Clostridium difficile in haematological malignancy ANITA RAMPLING, RE WARREN, PC BEVAN, CE HOGGARTH, D SWIRSKY, FGJ HAYHOE 445Serological identification of Streptococcus sanguis and Str mitior LYN C BALL............................................. 452Rapid diagnosis of vaginal candidosis by latex particle agglutination VALERIE HOPWOOD, EGV EVANS, JA CARNEY ............... 455Serum C-reactive protein concentration in the management of infection in patients treated by continuous ambulatory peritonealdialysis CRK HIND, SP THOMSON, CG WINEARLS, MB PEPYS ............................................................... 459

Can the rapid semiquantitative estimation of serum C reactive protein be adapted for the management of bacterial infection? NPRICHARDS, TS ELLIOTT, RJ POWELL, C O'CALLAGHAN, P FRANKLIN ...... ...................... ............................. 464

Normal reference ranges for biochemical substances relating to renal, hepatic, and bone function in fetal and maternal plasmathroughout pregnancy CF MONIZ, KH NICOLAIDES, FJ BAMFORTH, CH RODECK............................................... 468Rapid falls in maternal serum a-fetoprotein concentrations MT BREDOW, DJ GOLDIE........................................ 473Technical methodFresh frozen cryosupernatant in place of fresh frozen plasma for broad spectrum coagulation factor replacement JAF NAPIER, H BASS,R PENGILLEY ...................................................................................................... 475Letters to the Editor............................................................................................... 477Book reviews ................................................................................................ 479Notice............................................................................................... 480Some new titles ............................................................................................... 480

No 5 MAY 1985

Immunocytochemistry of folliculo-stellate cells of normal and neoplastic human pituitary gland CHRISTINE S MORRIS, ER HITCHCOCK 481Quantitative morphometry of squamous cell hyperplasia of the larynx P OLDE KALTER, H LUBSEN, JFM DELEMARRE, CL ALONS, RWVELDHUIZEN, CJLM MEYER, GB SNOW .................................................................................. 489

Morphometric analysis of nuclei in epithelial structures from normal and neoplastic endometrium: a study using the Isaacs cell samplerand Endoscann instruments ELSA SKAARLAND........................................................................ 496Nuclear size and shape of epithelial cells from the endometrium: lack of value as a criterion for differentiation between normal,hyperplastic, and malignant conditions ELSA SKAARLAND .............................................................. 502Mesoblastic nephroma contains fibronectin but lacks laminin S KUMAR, HB MARSDEN, T CARR, R KODET....................... 507Localisation of Ca and HMFG2 antigens in breast tissue by immunoperoxidase, immunofluorescence, and immunoelectronmicroscopy URSZULA BECKFORD, CALYPSO BARBATIS, JE BEESLEY, JANE C LINSELL, SHIREEN M CY.NTLER......................... 512Expression in normal adult, fetal, and neoplastic tissues ofa carbohydrate differentiation antigen recognised by antigranulocyte mousemonoclonal antibodies NOIRIN C MCCARTHY, JRM SIMPSON, G COGHILL, MA KERR ........................................... 521Clinical and histological features of delta infection in chronic hepatitis B virus carriers ASP LOK, I LINDSAY, PJ SCHEUER, HC THOMAS 530United Kingdom scheme for external quality assessment in virology. Part I. General method of operation SYLVIA E REED, PS GARDNER,JJS SNELL, OPHELIA CHAI.................... ; 534United Kingdom scheme for external quality assessment in virology. Part II. Specimen distribution, performance assessment, andanalyses of participants' methods in detection of rubella antibody, hepatitis B markers, general virus serology, virus identification, andelectron microscopy SYLVIA E REED, PS GARDNER, JUDITH STANTON....................................................... 542Comparison of ELISA with virus isolation for the diagnosis of genital herpes I ALEXANDER, CR ASHLEY, KJ SMITH, J HARBOUR, APCHROOME, JM DARVILLE ............................................................................................... 554Meningitis due to Gemella haemolysans after radiofrequency trigeminal rhizotomy RG MITCHELL, PJ TEDDY.................... 558Evaluation of Bactigen latex agglutination and Phadebact coagglutination for detection ofbacterial antigens in cerebrospinal fluid YMCOOVADIA, KK NAIDU............................................................................................... 561Effect of the growth of anaerobic bacteria on the surface pH of solid media B WATT, FIONA V BROWN........................ 565Proficiency testing in immunohaematology in Ontario, Canada, and in the United Kingdom: a comparative study PH PINKERTON,ED ZUBER, DE WOOD, AM HOLBURN, D PRIOR ........................................................................... 570Biphenotypic leukaemia: a case ofmixed T lymphoblastic and myeloblastic leukaemia MAM MORGAN, CS SCOTr, J TAVARES DE CASTRO,HU LIMBERT, N POLLU, D CATOVSKY, RD PYRAH, BE ROBERTS .............5.................................................575Lymphocyte subpopulations in patients with hydroxocobalamin responsive megaloblastic anaemia MA WODZINSKI, MJ FORREST, DBARNETT, ACK LAWRENCE .......................................................................................... 82Adenine nucleotide concentrations in patients with erythrocyte autoantibodies VF STRONG, RU SOKOL, SA RODGERS, S HEW1TT ...... 585Relation between raised concentrations of fucose, sialic acid, and acute phase proteins in serum from patients with cancer: choosingsuitable serum glycoprotein markers GA TURNER, AW SKILLEN, P BUAMAH, D GUTHREE, J WELSH, J HARRISON, A KOWALSKI ........ 588Technical methodEarlier detection of bacteraemia using conventional microbiological techniques ELIZABETH R YOUNGS, C ROBERTS........593Letters to the Editor............................................................................................... 594

Book reviews ................................................................................................ 597

No 6 JUNE 1985

Light chains in Mediterranean lymphoma PG ISAACSON, SK PRICE ....................................................... 601Immunocytochemical staining of T and B lymphocytes in serous effusions AK GHOSH, Al SPRIGGS, DY MASON ..... ............. 608Intestinal metaplasia in endoscopic biopsy specimens of gastric mucosa GA ROTHERY, DW DAY .............................. 613

Morphological range of hyperplastic polyps and carcinomas arising in hyperplastic polyps of the stomach T HATTORI .... ...... 622

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Quantitation in inflammatory bowel disease using computerised interactive image analysis EM THOMPSON, AB PRICE, DG ALTMAN, CSOWTER, G SLAVIN ................................................................................................. 631Gastricsin in the benign and malignant prostate WA REID, CN LIDDLE, J SVASTI, J KAY ...................................... 639Endometrial lymphoid tissue: an immunohistological study H MORRIS, J EDWARDS, A TILTMAN, M EMMS ..... .................. 644Hepatic copper distribution in primary biliary cirrhosis shown by the scanning proton microprobe DJT VAUX, F WATT, GW GRIME,JTAKACS ........................653............................................................................653Vascular occlusion and infarction in sickle cell crisis and the sickle chest syndrome NA ATHANASOU, C HATTON, JO'D MCGEE, DJWEATHERALL...................................................................................................... 659Investigation of a kindred with a new autosomal dominantly inherited variant type von Willebrand's disease (possible type IID) FGHHILL, MS ENAYAT, AJ GEORGE ......... .............................................................................. 665

Modified immunocytochemical slide technique for demonstrating surface antigens on viable cells N FRICKHOFEN, KJ BROSS, W HEIT,H HEIMPEL............................................................. .......................................... 671Non-suppression of cortisol secretion by long term treatment with ketoconazole in patients with acute leukaemia P DANDONA, JMOHIUDDIN, HG PRENTICE ........................................................................................... 677Detection of specific IgG and IgM antibodies to Toxoplasma gondii with a commercially available enzyme immunoassay kitsystem AH BALFOUR, JP HARFORD ................................................................................... 679Evaluation of gas-liquid chromatography for the rapid diagnosis of Clostridium difficile associated disease PAOLA GIANFRILLI,ANNALISA PANTOSTI, IDA LUZZI ..................................................................................... 690Detection of rotavirus by a latex agglutination test, Rotalex: comparison with electron microscopy, immunofluorescence,polyacrylamide gel electrophoresis, and enzyme linked immunosorbent assay RB MOOSAI, R ALCOCK, TM BELL, FR LAIDLER, JSM PEIRIS,AP WYN-JONES, CR MADELEY ......................................................................................... 694Use of immunoblotting to identify antigenic differences between the yeast and mycelial phases of Candida albicans JP BURNIE, RUTHC MATTHEWS, A FOX, SOAD TABAQCHALI .............................................. ................................ 701Acute placentitis and spontaneous abortion caused by Chiamydia psittaci of sheep origin: a histological and ultrastructural study syWONG, ES GRAY, D BUXTON, J FINLAYSON, FWA JOHNSON ................................................................. 707Technical methodDirect immunoperoxidase method for demonstrating Chiamydia psittaci in tissue sections 1 FINLAYSON, D BUXTON, IE ANDERSON, KMDONALD.......................................................................................................... 712Letters to the Editor............................................................................................... 715Book reviews ................................................................................................. 717Notices ................................................................................................. 720Some new titles ................................................................................................. 720

No 7 JULY 1985

Infections in British clinical laboratories 1982-83 NR GRIST, JAN EMSLIE ................................................... 721Chlamydial endometritis J PAAVONEN, R AINE, K TEISALA, P HEINONEN, R PUNNONEN, M LEHTINEN, A MEETTINEN, P GRONROOS ...... 726Detection of IgM antibodies against Chlamydia trachomatis by enzyme linked fluorescence immunoassay K NUMAZAKI, S CHIBA, TYAMANAKA, T MOROBOSHI, K AOKI, T NAKAO .....................733Enzyme immunoassay for the detection of Chlamydia trachomatis antigen in urethral and endocervical swabs GILANFAR MUMTAZ, BJMELLARS, GL RIDGWAY, JD ORIEL ..................................................................................... 740Changes in circulating immune complex concentrations and antibody titres during treatment in Q fever endocarditis PV COYLE, i

THOMPSON, AAJ ADGEY, DA RUTTER, A FAY, TA MCNEILL, JH CONNOLLY...................................................... 743Phagocytosis and killing of Gardnerella vaginalis by human neutrophils CSF EASMON, L CLARK, JP CRANE, RGREEN.............. 747Distribution ofbiotypes of Haemophilus influenzae and Hparainfluenzae in patients with cystic fibrosis KC WATSON, EJC KERR, CA MINKS 750Histopathological classification and malignant change in gastric polyps T NAKAMURA, G-I NAKANO........................... 754Quantitative assessment of the mucosal architecture of jejunal biopsy specimens: a comparison between linear measurement,stereology, and computer aided microscopy GR CORAZZA, M FR4ZZONI, HF DIXON, G GASBARRINI ............................. 765Density of mucosal mast cells in the lamina propria of the colon and terminal ileum of children IR SANDERSON, G SLAVIN, JAWALKER-SMITH .................................................................................................... 771A quantitative study of immunoglobulin containing cells in the differential diagnosis of acute colitis JP VAN SPREEUWEL, J LINDEMAN,CJLM MEIJER ...................................................................................................... 774Histological features of sclerosing cholangitis in patients with chronic ulcerative colitis C BARBATIS, P GRASES, HA SHEPHERD, RWCHAPMAN, JOAN TROWELL, DPJ JEWELL, JO'D MCGEE ...... ............................................................... 778An immunohistochemical study of branchial cysts JCROC KER,RACHEL JENKINS.............................................784Factors influencing the survival of patients with testicular teratoma JOAN M ALDERDICE, JD MERRETT ..... .................... 791Isolated arteritis of the epididymis C WOMACK, ID ANSELL .............................................................. 797Immunomorphological characterisation of antinuclear antibodies in chronic liver disease F CASSANI, FB BIANCHI, M LENZI, U VOLTA,E PISI ............................................................................................................ 801Demonstration of serum monoclonal immunoglobulin in a case of non-secretory myeloma by immunoisoelectric focusing T SHEEHAN,D SINCLAIR, P TANSEY, JR O'DONNELL ................................................................................. 806Thalassaemia trait and pregnancy JM WHITE, R RICHARDS, M BYRNE, T BUCHANAN, YS WHITE, G JELENSKI ..... ................. 810Pleural fluid ferritin concentrations in human disease M KLOCKARS, T WEBER, P TANNER, P-E HELLSTROM, T PETTERSSON .... ...... 818Platelet release protein which inhibits plasminogen activators NUALA A BOOTH, JUDITH A ANDERSON, B BENNETT .... ............ 825Extramedullary transformation of chronic myeloid leukaemia with a unique chromosome pattern NG FLANAGAN, CJ HARRISON, DSHARRY, JC RIDWAY................................................................................................. 831

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Technical methodSimplified procedure for tissue culture in routine detection of cytotoxins AR WELCH, SP BORRIELLO, FIONA E BARCLAY .... ....... 835Letters to the Editor............................................................................................... 838Book reviews ................................................................................................ 844Notices ................................................................................................ 844

No 8 AUGUST 1985

Role of immunohistochemistry in diagnosis of nasopharyngeal tumours J BOSQ, KC GATTER, C MICHEAU, DY MASON.............. 845Distribution of fibronectin and laminin in normal and pathological lymphoid tissue JTREILLY, JRNASH, MJ MACKIE, BA MCVERRY.. 849Comparison of prevalence ofhuman papillomavirus antigen in biopsies from women with cervical intraepithelial neoplasia A SINGER,JWILTERS, P WALKER, D JENKINS, G SLAVIN, H COWDELL, A TO, 0 HUSAIN ..................................................... 855Retrospective study of histological features of acute rejection in renal allografts and comparison with circulating T cellpopulations J WYATT, SR APARICIO, PGUILLOU ........................................................................ 858Acquired cystic disease of kidney with multiple renal and urothelial neoplasms SS BANERJEE, M HARRIS, EW LUPTON, P ACKRILL .... 864Clinicopathological importance of deposits of amyloid in the femoral head NRB CARY...................................... 868Aortic valvular tophus: identification by X-ray diffraction of urate and calcium phosphates JMGAWOSKI, K BALOGH, WJLANDIS .. 873Hepatic pseudolipoma PJ KARHUNEN................................................................................ 877Clear cell carcinoma of the lung C EDWARDS, A CARLILE ................................................................ 880Automated screening of cervical smears using immunocytochemical staining: a possible approach BILYANA VALKOVA, DJR LAURENCE 886Lymphoid and epithelial markers in small cell anaplastic thyroid tumours AD BURT, DJ KERR, IL BROWN, P BOYLE .... ........... 893Intracytoplasmic inclusions in B prolymphocytic leukaemia; ultrastructural, cytochemical, and immunological studies DSF ROBINSON,JV MELO, C ANDREWS, SA SCHEY, D CATOVSKY ............................................................................ 897Morphometry of blood monocytes in malignant lymphoma RJ SOKOL, G HUDSON, J WALES, NT JAMES ..... ..................... 904Immune abnormalities in myelodysplastic syndromes T ECONOMOPOULOS, J ECONOMIDOU, G GIANNOPOULOS, C TERZOGLOU,E PAPAGEORGIOU, J DERVENOULAS, P ARSENI, J HADJIOANNOU, S RAPTIS ....................................................... 908Enzyme linked direct antiglobulin tests in patients with autoimmune haemolysis RI SOKOL, S HEWITT, DJi BOOKER, R STAMPS....... 912Electron microscopic studies of human haemosiderin and ferritin MP WEIR, GA SHARP, TJ PETERS.............................. 915Detection of fetomaternal haemorrhage by an immunofluorescence technique AC MCWILLIAM, SALLY C DAVIES ..... ............. 919Automation and limitation of 17 f oestradiol dehydrogenase in monitoring ovarian function DL HAY, GF IBRAHIM, IHORACEK .... 921Evaluation of Malthus 1 28H microbiological growth analyser for detecting significant bacteriuria TK SMITH, R EGGINGTON, AA PEASE,DM HARRIS, RC SPENCER ............................................................................................ 926Comparative sensitivity of different serological tests for detecting chlamydial antibodies in perihepatitis MIRJA PUOLAKKAINEN,PEKKA SAIKKU, MAUA LEINONEN, MARJATTA NURMINEN, PERTTI VAANANEN, P HELENA MAKELA .................................... 929Analysis by gas liquid chromatography of production of volatile fatty acids by anaerobic bacteria grown on solid medium RI WIGGINS,M WILKS, S TABAQCHALI ............................................................................................ 933Misuse and interlaboratory test reproducibility of API 20E system B HOLMES, CA DAWSON................................... 937Indifferent streptococci in normal and purulent eyes of neonates JC REEDER, AJ WESTWELL, DN HUTCHlNSON ..... ............... 942Microbial and metabolic profile of achlorhydric stomach: comparison of pernicious anaemia and hypogammaglobulinaemiaSP BORRIELLO, PJ REED, JM DOLBY, FE BARCLAY, ADB WEBSTER .............................................................. 946Technical methodMicrocomputer program for cell counting I CLARK, OHB GYDE, D HOLTOM................................................. 954Letters to the Editor............................................................................................... 959Book reviews ................................................................................................ 962Notices ................................................................................................ 963

No 9 SEPTEMBER 1985

Review articleErythrocyte rheology J STUART ..................................................................................... 965Haematological and haemorheological changes associated with cigarette smoking G GALEA, RJL DAVIDSON ..... ................ 978Splenic pathology in immune thrombocytopenia MM HAYES, P JACOBS, LUCILLE WOOD, DM DENT ............................. 985Double staining immunofluorescence procedure for enumeration of cells containing cytoplasmic immunoglobulin in human bonemarrow: 40 selected cases MJ AYLIFFE............................................................................... 989Membrane phenotypic studies in B cell lymphoproliferative disorders CS SCOTT, HJ LIMBERT, ID MACKARILL, BE ROBERTS......... 995Expression of the common acute lymphoblastic leukaemia antigen (CALLA gp 100) in the brush border of normal jejunum andjejunum of patients with coeliac disease LK TREJDOSIEWICZ, G MALIZIA, JANE OAKES, MS LOSOWSKY, G JANOSSY.................. 1002Primary biliary cirrhosis and chronic active hepatitis: an examination of clinical, biochemical, and histopathological features indifferential diagnosis JMS WILLIAMSON, DM CHALMERS, AD CLAYDEN, MF DIXON, WSJ RUDDELL, MS LOSOWSKY.................... 1007Structural aspects of the liver in patients with biliary disease and portal hypertension K WEINBREN, NS HADJIS, LH BLUMGART .... 1013Cells which contain S-100 protein in Hodgkin's disease: a quantitative study G SANGSTER, J CROCKER, RACHEL JENKINS, Mi LEYLAND 1021Morphometric analysis of atrophic changes in human lingual epithelium in iron deficiency anaemia i SCOTT, JA VALENTINE, CA ST HILL,CR WEST ......................................................................................................... 1025Small vessel disease in progressive diabetic neuropathy associated with good metabolic control WR TIMPERLEY, AJM BOULTON, GABDAVIES-JONES, JA JARRATT, JD WARD .................................................................................. 1030

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Disseminated zygomycosis associated with erythroleukaemia: confirmation by lectin stains EW BENBOW, IW DELAMORE, RW STODDART,H REID ........................................................................................................... 1039IgG enzyme linked immunosorbent assay for diagnosis of invasive aspergillosis: retrospective study over 15 years of transplantrecipients AK TRULL, J PARKER, RE WARREN ....... ................................................................... 1045Improved method for isolation and growth of Chlamydia trachomatis in McCoy cells treated with cycloheximide using polyethyleneglycol NOORIA RS MOHAMMED, IRENE B HILLARY ....... ................................................................ 1052

Effect of temperature on antimicrobial susceptibilities of Pseudomonas maltophilia PF WHEAT, TG WINSTANLEY, RC SPENCER...... 1055Screening tests for antibodies to cytomegalovirus: an evaluation of five commercial products PCA GRINT, CJ RONALDS, HO KANGRO,A CAMPBELL-BENZIE, F WARD, AE HARDIMAN, RB HEATH .................................................................. 1059

Particulate fraction in amniotic fluid at second trimester H JALANKO, J RAPOLA, E LEHTONEN ................................ 1065Technical methodUse of microwaves for acid and alcohol fast staining S HAFIZ, RC SPENCER, MARGARET LEE, HILARY GOOCH, BI DUERDEN .... ..... 1073Letters to the Editor............................................................................................... 1076Book reviews ................................................................................................. 1083Notices ................................................................................................. 1084

No 10 OCTOBER 1985

Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells J BURNS, VTWCHAN, JA JONASSON, KA FLEMING, SUSAN TAYLOR, JO'D MCGEE ............................................................. 1085Recombinant DNA studies on stored necropsy brain samples from patients with Huntington's chorea MEENA UPADHYAYA, GPREYNOLDS, PS HARPER .............................................................................................. 1093

Comparison of DNA flow cytometry from fresh and paraffin embedded samples of non-Hodgkin's lymphoma RS CAMPLEJOHN, JCMACARTNEY...................................................................................................... 1096Follicular thyroid tumours: a study of laminin and type IV collagen in basement membrane and endothelium CH KENDALL,PR SANDERSON, JANET COPE, IC TALBOT ............... ................................................................... 1100

Microtome: histopathology day book based on a microcomputer system D ROBERTS-JONES, J MCCLURE........................ 1106Parathyroid carcinoma associated with chronic renal failure and previous radiotherapy to the neck JP IRELAND, SJ FLEMING, DALEVISON, WR CATTELL, LRI BAKER .................................................................................... 1114

Defining duodenitis: quantitative histological study of mucosal responses and their correlations D JENKINS, A GOODALL, FR GILLET,BB SCOTT .......................................................................................................... 1119

Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastrnc mucosa CS GOODWIN, EDBLINCOW, JR WARREN, TE WATERS, CR SANDERSON, L EASTON.............................................................. 1127Four hour identification of Enterobacteriaceae with the API Rapid 20E and Micro-ID systems B HOLMES, PS HUMPHRY......... 1132Enzyme amplified immunoassay: a novel technique applied to direct detection of Chiamydia trachomatis in clinical specimens SFPUGH, RCB SLACK, EO CAUL, ID PAUL, PN APPLETON, S GATLEY ............................................................ 1139

Evaluation of a direct immunofluorescence test for diagnosing gonorrhoea CA ISON, K MCLEAN, J GEDNEY, PE MUNDAY, D COGHILL,R SMITH, JRW HARRIS, CSF EASMON.................................................................................... 1142More rapid identification of bacteraemia by manual rather than radiometric method LEELA A GANGULI, MAEVE GL KEANEY, WA HYDE,SUSAN B FRASER .................................................................................................. 1146

Public Health Laboratory Service IgM antibody capture enzyme linked immunosorbent assay for detecting rubella specificIgM KATHRYN BELLAMY, J HODGSON, PS GARDNER, P MORGAN-CAPNER .................................................... 1150

Alkaline phosphatase immunoenzymatic staining for detection of antigens induced by cytomegalovirus MONICA MUSIANI, MARIALUISAZERBINI, M LA PLACA .............................................................................................. 1155Comparison of an avidin-biotin immunoassay with three commercially available immunofluorescence kits for typing of herpes simplexvirus DL BARNARD, FB JOHNSON, DF RICHARDS ....................................................................... 1158

Unusually high incidence of tuberculosis among boys with haemophilia during an outbreak of the disease in hospital AC BEDDALL,FGH HILL, RH GEORGE, MD WILLIAMS, K AL-RUBEI ....................................................................... 1163Fibrinogen mediated activation of platelet aggregation and thromboxane A2 release: pathological implications in vascular disease DPMIKHAILIDIS, MA BARRADAS, A MARIS, JY JEREMY, P DANDONA ............................................................ 1166Size of spleen rather than amount of platelet sequestration may determine long term responses to splenectomy in adult idiopathicthrombocytopenic purpura BJ BOUGHTON, P SMITH, J FIELDING, R HAWKER, I WILSON, S CHANDLER, A HOWIE.................... 1172Defective activation of neutrophils after splenectomy PN FOSTER, RP BOLTON, KL COTTER, MS LOSOWSKY....................... 1175Importance of low serum vitamin B12 and red cell folate concentrations in elderly hospital inpatients EL BLUNDELL, JH MATTHEWS,SM ALLEN, AM MIDDLETON, JE MORRIS, SN WICKRAMASINGHE............................................................... 1179Changes in plasma fibronectin during allogeneic bone marrow transplantation DR NORFOLK, M BOWEN ..... .................. 1185Zinc deficiency in senile purpura NY HABOUBI, NUHA AA HABOUBI, OHB GYDE, NA SMALL, AV BARFORD ..... .................... 1189Safety of intravenous immunoglobulin treatment HI ATRAH, RJ CRAWFORD, BC DOW, RUTHVEN MITCHELL ...................... 1192Technical methodsCell suspension culture for studying development of macrophages RJ SOKOL, J WALES, G HUDSON ............................ 1194Donor screening for antibody to hepatitis B surface antigen SUSAN M LAKE, AY PUCKETT, CC ENTWISTLE ..... ................. 1195Letters to the Editor................................................................................................ 1196Book reviews ................................................................................................. 1199Correction.................................................................................................. 1200

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No It NOVEMBER 1985

Review articleMyelodysplastic syndromes: pathogenesis, functional abnormalities, and clinical implications A JACOBS....................... 1201Aplastic anaemia and the hypocellular myelodysplastic syndrome: histomorphological, diagnostic, and prognostic features INGRIDFOHLMEISTER, R FISCHER, B MODDER, M RISTER, H-E SCHAEFER.............................................................. 1218Presence of epidermal growth factor receptor as an indicator of poor prognosis in patients with breast cancer JRC SAINSBURY, AJMALCOLM, DR APPLETON, JR FARNDON, AL HARRIS....................................................................... 1225Monitoring serum CEA in women with primary breast tumours positive for oestrogen receptor and with spread to lymph nodes JCVAN DER LINDEN, JPA BAAK, T POSTMA, J LINDEMAN, CJLM MEYER .......................................................... 1229Frozen section diagnosis: an audit EK DANKWA, JD DAVIES ............................................................. 1235Granulocyte and HLA-D region specific monoclonal antibodies in the diagnosis of Hodgkin's disease AJ NORTON, PG ISAACSON 1241Fertility in young men and women after treatment for lymphona: a study of a population DJ KING, MA RATCLIFFE, AA DAWSON, BBENNETT, JE MACGREGOR, Al KLOPPER ................................................................................. 1247Necrotising lymphadenitis without granulocytic infiltration (Kikuchi's disease) MH ALI, LWL HORTON......................... 1252Comparison of # enolase and myoglobin as histological markers of rhabdomyosarcoma JANICE A ROYDS, S VARIEND, WR TIMPERLEY,CB TAYLOR ....................................................................................................... 1258Ploidy studies in adenomatous polyps of the colon F WHITEHEAD, LR JARVIS, JM SKINNER, R WHITEHEAD....................... 1261Oesophageal histology in reflux oesophagitis BJ COLLINS, H ELLIOTT, JM SLOAN, RJ MCFARLAND, AHG LOVE .... ................. 1265Basal cell carcinoma of the skin with areas of squamous cell carcinoma: a basosquamous cell carcinoma? LJ LOPES DE FARIA ... 1273Solitary "necrotic nodule" of the liver: a probable pathogenesis CL BERRY ............................................... 1278Antibodies specific for measles virus envelope antigens and autoantibodies in patients with chronic active hepatitis PV SHIRODARIA,SA MCMILLAN, ME CALLENDER, MARGARET HAIRE, ELIZABETH FLEMING, JD MERRETT, TT FULTON.................................. 1281Rapid identification of thermophilic Naegleria, including Naegleria fowleri using API ZYM system S KILVINGTON, DG WHITE .... 1289"Agrobacterium yellow group" and Pseudomonas paucimobilis causing peritonitis in patients receiving continuous ambulatoryperitoneal dialysis RA SWANN, SJ FOULKES, B HOLMES, JB YOUNG, RG MITCHELL, ST REEDERS................................... 1293Immunoblot analysis of serological responses in invasive aspergillosis RUTH MATTHEWS, JP BURNIE, A FOX, S TABAQCHALI ........ 1300Rapid enzyme analysis of amniotic fluid phospholipids containing choline: a comparison with the lecithin to sphingomyelin ratio inprenatal assessment of fetal lung maturity SH TENG, AG ANDREWS, I HORACEK ............................................. 1304Increased total serum IgE concentration in patients who have undergone splenectomy after trauma G CHELAZZI, G PINOTTI, C NICORA,D ROSSI, G SENALDI................................................................................................. 1309Technical methodsEconomic cultivation of "thermophilic" Campylobacter spp CD RIBEIRO, J MARKS, AD GRIMSHAW............................. 1311An easy way to orientate small muscle biopsy tissue PC so ............................................................. 1312Letters to the Editor............................................................................................... 1313Book reviews .................................................................................................... 1319Notices ..................................................................................................... 1319Some new titles ................................................................................................... 1320

No 12 DECEMBER 1985

Review artic leCryptosporidium species a "new" human pathogen DP CASEMORE, RL SANDS, A CURRY ..................................... 1321Laboratory diagnosis of cryptosporidiosis DP CASEMORE, M ARMSTRONG, RL SANDS ......................................... 1337Leucocyte esterase-nitrite screening method for pyuria and bacteriuria EGL WILKINS, JG RATCLIFFE, C ROBERTS .... ............. 1342Improving detection of Viridans streptococcus bacteraemia by adding sodium polyanethol sulphonate to blood cultures DC SHANSON,FD THOMAS, D JOHNSTONE ........................................................................................... 1346

Role of folate dependent transformylases in synthesis of purine in bone marrow of man and in bone marrow and liver ofrats ROSEMARY DEACON, I CHANARIN, M LUMB, JANET PERRY ............................................................. 1349An immunocytochemical study of malignant melanoma and its differential diagnosis from other malignant tumours KC GATTER,E RALFKIAER, J SKINNER, D BROWN, A HERYET, KAF PULFORD, K HOU-JENSEN, DY MASON ....................................... 1353Observer variability in reporting of breast histopathology J SWANSON BECK AND MEMBERS OF THE MEDICAL RESEARCH COUNCIL BREASTTUMOUR PATHOLOGY PANEL ASSOCIATED WITH THE UNITED KINGDOM TRIAL OF EARLY DETECTION OF BREAST CANCER................ 1358Biopsy findings in cases of rejection of liver allograft SG HUBSCHER, D CLEMENTS, E ELIAS, P MCMASTER ......................... 1366New method for measuring intimal component of pulmonary arteries JUNE M FERNIE, D LAMB ................................ 1374Method for maximising measurements of muscular pulmonary arteries JUNE M FERNIE, D LAMB ............................... 1380Growth of crypt cell nodules in duodenal mucosa in man during organ culture in vitro DN CHALLACOMBE, EE WHEELER.......... 1388Production of necropsy reports by medical staff using a computer system AG BROWN, CL BERRY.............................. 1394Comparative study of three methods of plastic embedding in diagnostic dermatopathology MARY MASON, RONA M MACKIE........ 1397Technical methodTechnique for removal of the vertebral arteries A BROMILOW, J BURNS .................................................... 1400Letters to the Editor............................................................................................... 1403Book reviews .................................................................................................... 1408List of Assessors .................................................................................................. 1410Annual Author Index to Volume 38.................................................................................. 1411Annual Subject Index to Volume 38.................................................................................. 1417Annual Contents to Volume 38..................................................................................... 1431

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