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Transcript of Jordan Garth portfolio
JORDAN GARTH UNDERGRADUATE PORTFOLIO
A summary of a few important projects done while in undergraduate at Georgia Institute of Technology
1
Table of Contents • Lab for Neuroengineering à Slide 3 • BMED 2300 Problems in Engineering II (Sophomore
Design) à Slide 13 • GTRI ELSYS Co-op II Summer 2014 à Slide 35 • GTRI EOSL Co-op III Spring 2015 à Slide 54
2
Lab for Neuroengineering • I worked in the Lab for Neuroengineering from January
2012-December 2012 • The following slides are a summary of my work from that
time
Lab for Neuroengineering
3
U87MG-PE-GFP CANCER CELL LINE IN VITRO
Jordan Garth Undergraduate Research Fall 2012
Georgia Institute of Technology Bellamkonda Lab for Neuroengineering
Mentor: Martha Betancur November 28, 2012
Lab for Neuroengineering
4
Research This Semester • DARPA Project
• Researched the failure mechanisms of neuronal electrodes • Sectioning • Staining • Imaging • Image Analysis
• Eureka Project • ‘Exvading’ inoperable brain tumors in the cerebellum • Goal à to engineer a path of least resistance in using PCL
nanofiber conduits for treatment
Lab for Neuroengineering
5
Using the U87MG-pe-GFP Cell Line
• Why do we use U87MG-pe-GFP cancer cell line? • Able to inoculate in vivo and measure the tumor that develops
• Common human glioblastoma cell line Tissue from an inoculated animal
Lab for Neuroengineering
6
Testing Ki67 and PCNA on U87 Cells • We cultured the U87 cells into the slides to test Ki-67 and
PCNA • Ki-67 is a protein used to measure proliferation of the cells
• Present in all cells life cycles except for G0 • PCNA is a protein present during DNA synthesis • Goal à calculate proliferation rate in the cells
Lab for Neuroengineering
7
Cell Culture Overview
• Incubate PS – 2 slides • Make complete media • Put frozen cells into the water broth • Cells adhere to the bottom • Washed with PBS, add trypsin to pick up the cells • Place in new media for an allotted time
Lab for Neuroengineering
8
Imaging
Ki67
GFP
DAPI
Merged image of Ki67 10X (48 hr) Merged image of PCNA 10X (48 hr)
Lab for Neuroengineering
9
Image Analysis
0
10
20
30
40
50
60
70
Cell Count
Dapi cell Count from Ki67
GFP cell Count from Ki67
Dapi cell Count from PCNA
GFP cell Count from PCNA
Average Cell Count
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
Index (%cells not in G0)
Ki67 index From Dapi
Ki67 index from GFP
PCNA index from Dapi
PCNA index from GFP
Percentage of Cells Proliferating
Index = (Cells with proteins)/(Total cells detected with DAPI or GFP)
N=9 images per staining
Lab for Neuroengineering
11
Future Studies
• It is now clear to use Ki-67 to detect proliferation in U87MG-pe-GFP
• Next step à use this stain in vivo sections • Get the proliferation rate using Ki-67 in U87MG-pe-
GFP in the empty versus the aligned nanofilm conduits
• I suspect that the proliferation rate of the cells is hypothesized to be less in vivo than in vitro • In vitro the cells have much more media to feed off of,
whereas in vivo, the tumor cells are more starved
Lab for Neuroengineering
12
BMED 2300: Problems in Biomedical Engineering II (Design Course) • The objective of the course was to choose a medical
device to redesign to better fit a user’s needs • This is a sophomore design course taken in a group,
which I took in Spring 2013
BMED 2300
13
Introduction • Football helmets prevent
the probability of sustaining brain injury • Designed to absorb forces
upon impact and slow down the reaction
• Strategically placed padding • Concussions are a
commonality amongst football players
• Significant metabolic derangement that occurs immediately after a concussion
BMED 2300
14
• High school football player with special conditions • I.e. mood disorders,
previous concussions, physical abnormalities
• Require specific needs • Easier access • Increased protection
Users
http://photos.pennlive.com/patriot-news/2012/08/2012_high_school_football_play_18.html
http://upload.wikimedia.org/wikipedia/commons/6/6e/MRI_of_human_brain_with_type-1_Arnold-Chiari_malformation_and_herniated_cerebellum.jpg
Individual with Arnold Chiari Malformation
BMED 2300
15
Problem Statement • With more than 7.6 million high school students play
football, with average player sustains an estimated 950 impacts to the head during a season
• Both athletic trainers and athletes feel significant pressure to return athletes to practice and play as soon as possible after injury
• Statement: Redesign the football helmet with increased protection and ease of access
BMED 2300
16
Objectives • Absorb the shock from hits, and prevent the most amount
of damage possible • Lower probability of concussions and brain injury • Meet NOCSAE regulations • Provide cost efficiency, durability, be aesthetically pleasing
and lightweight
BMED 2300
17
Iterations
Kevlar Layer
• Pros: Shock absorption, energy dissipation
• Cons: Cost inefficient, friction if it is too thick
Thicker Pads, Larger Helmet
• Pros: More protection, less impact
• Cons: Additional weight, decreased peripherals
BMED 2300
21
Iterations
Pull Apart Helmet
• Pros: Ease of Access • Cons: Cost, Could open
up during game play
Light Sensor
• Pros: Detects size, perfect fit
• Cons:Extra Cost
BMED 2300
22
Iterations
Hinged Sides
• Pros: Easy access, comfortable fit
• Cons: Added weight, could fall off with overuse
Hinged Facemask
• Pros: Easy Access • Cons: Extra Cost,
Facemask could come off
BMED 2300
23
Iterations
Rubber Stoppers with Internal Cap
• Pros: Added protection and firm placement
• Cons: Cost, less space for head inside of helmet
Rubber Stoppers
• Pros: Firm placement • Cons: Cost
BMED 2300
24
Iterations
Spiderweb padding system
• Pros: Distributes force evenly, provides better fit
• Cons: More material means more expensive
BMED 2300
25
Experienced User Interview • "As a former football player who experienced multiple concussions, I think the network of pads under a layer of Kevlar will significantly help reduce impact force and allow players whose conditions could be worsened by head injury to play like normal.“
-Taylor Nicholson
• "Being a trainer, I know that accessibility to an injured player is important. Though the hinged sides may make it too easy for the helmet to come off during play, the hinged face mask provides just the right amount of accesibility for a trainer to attend to player who needs help."
-Dana Domangue BMED 2300
27
P3 Final Design • Hinged Facemask • Spiderweb Padding System • Thin Layer of Kevlar • Rubber Stoppers
BMED 2300
28
Projects • Balance study in conjunction with Georgia Tech using
ballerinas and novices • ISSS working on patient status monitor
36
Project 1: Ballerinas • In this study:
• The subject wears markers that reflect from LED infrared lights to show position in space
• GTRI provides accelerometer to find orientation of the dancer and acceleration in the body frame
• Current contribution: • Installation of sensors, data collection, troubleshooting, and general
support
37
Project 1: Ballerinas cont. • Future contribution: data analysis
• Goal from the data is to get the overall body acceleration from the body frame angular rate and body frame angular acceleration
Using the laws of motion in a rotating reference frame
38
Project 2: ISSS PSM Feedback • Wearable devices on the wrist are popular (solar watches) • Charging strips are limited on deployment (lasting up to days
at a time), and therefore power could make some solutions impractical
• Adidas was through to be uncomfortable due to its wide form factor
• Overall, it was decided that preferences would lean towards a BASIS functionality in a Mio profile with a Mio band.
• Some concern exists that larger watch faces will snag of cuff buttons and be difficult to access
• Gloved operation was not an issue, although the BASIS was preferred because one could be less precise.
40
Project 2: ISSS PSM Testing • Basis versus Mio Alpha compared on pulse oximeter • Resting HR Procedure:
• Put both on approx at same time, Wait 1 min, Record • Elevated HR procedure:
• 20 jumping jacks, Wait 15 seconds, Record
42
Project 2: ISSS PSM conclusion 1 • Mio Alpha based on its superior accuracy is the best
watch • Now onto additional testing and redesigns
44
Final testing: Mio Alpha • Test that simulates soldier conditions:
• Resting HR for 30 minutes • 50% HR (for 20-30 year olds, this is between 100-130 bpm) • 80% HR (for 20-30 year olds, this is between 150-180)
45
Resting Heart Rate
0
10
20
30
40
50
60
70
80
90
100
10:3
6:51
10
:37:
41
10:3
9:11
10
:40:
01
10:4
1:21
10
:42:
51
10:4
4:01
10
:45:
21
10:4
6:31
10
:47:
51
10:4
9:11
10
:50:
21
10:5
1:31
10
:52:
21
10:5
4:41
10
:56:
21
10:5
7:51
10
:59:
11
11:0
0:01
11
:01:
31
11:0
2:51
11
:04:
41
11:0
6:01
11
:07:
01
Mio HR
GE HR
Kyle Land Resting HR
Average Error: 4.992092
0
10
20
30
40
50
60
70
80
90
100
14:4
4:43
14
:46:
03
14:4
7:03
14
:48:
13
14:4
9:03
14
:49:
53
14:5
1:03
14
:52:
13
14:5
3:13
14
:54:
23
14:5
5:13
14
:56:
23
14:5
7:33
14
:58:
33
15:0
0:04
15
:01:
03
15:0
2:13
15
:03:
13
15:0
4:04
15
:05:
33
15:0
6:33
15
:07:
34
15:0
8:33
15
:09:
33
15:1
0:33
15
:12:
23
15:1
3:14
15
:14:
13
15:1
5:13
John McClure Resting HR
mio ge
Average Error 4.621705
46
50% Heart Rate
0
20
40
60
80
100
120
140
11:1
2:59
11
:13:
39
11:1
3:59
11
:14:
29
11:1
4:49
11
:15:
09
11:1
5:29
11
:15:
49
11:1
6:09
11
:16:
49
11:1
7:19
11
:17:
39
11:1
7:59
11
:18:
19
11:1
8:39
11
:19:
09
11:1
9:29
11
:19:
49
11:2
0:19
11
:20:
39
11:2
0:59
11
:21:
29
11:2
1:49
11
:22:
09
11:2
2:29
11
:22:
59
Kyle Land Medium HR
mio ge
Average percent error: 1.418672
0
20
40
60
80
100
120
140
160
John McClure HR
Mio GE
2.139142 Average percent error:
47
80% Heart Rate
0
20
40
60
80
100
120
140
160
180
11:2
6:04
11
:26:
24
11:2
6:34
11
:26:
44
11:2
7:34
11
:27:
44
11:2
7:54
11
:28:
43
11:2
9:04
11
:29:
14
11:2
9:34
11
:29:
44
11:2
9:53
11
:30:
04
11:3
0:14
11
:30:
24
11:3
0:44
11
:30:
54
11:3
1:03
11
:31:
14
Kyle Land
MIO GE
Average percent error: 1.971294
0
20
40
60
80
100
120
140
160
180
200
11:0
0:47
11
:00:
57
11:0
1:06
11
:01:
17
11:0
1:27
11
:01:
36
11:0
1:57
11
:02:
06
11:0
2:37
11
:02:
46
11:0
3:07
11
:03:
16
11:0
3:26
11
:03:
37
11:0
3:47
11
:03:
56
11:0
4:26
11
:04:
36
11:0
4:47
11
:04:
57
11:0
5:06
11
:05:
17
11:0
5:27
11
:05:
36
11:0
5:46
John McClure
Average percent error: 2.692379
48
Future designs and contributions • May consider other designs after thinking about the
logistics • Incorporating better battery life • Smaller fuel cell • Soldier feedback
53
Wheelchair Redesign • Current project in conjunction with Children’s Healthcare
of Atlanta • Improving a wheelchair for children with special needs that are
mostly immobile, but not paralyzed • Design specifications include: ability to rise and fall to heights of
different tables, to be lightweight, affordable, and foldable
• The upcoming design is the first iteration of a project over the next coming months • Will be in production for the upcoming months
54
Solidworks of Redesign 2: First iteration 57
• Folds in half • 3 wheels for a sporty and youthful aesthetic • Raises from the back using concentric cylinders