Johnny Yin 18 April, 2014 Expert of yeast origin nutrients for “Microorganisms”
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Transcript of Johnny Yin 18 April, 2014 Expert of yeast origin nutrients for “Microorganisms”
Johnny Yin
18 April, 2014
Johnny Yin
18 April, 2014
Expert of yeast origin nutrients for “Microorganisms”
Contents
1
2
3
Introduction about Angel yeast Co.,Ltd.
Angel yeast extracts – Good nutrient-sources for microoganisms
Application of Angel yeast extract
1
1. Introduction of Angel Yeast Co.,Ltd.1.1 About us >1.2 Global net work > 1.3 Our history >1.4 Main business >1.5 Angel’s value chain >1.6 Employees >1.7 Advantages >
1.1 About us
• Angel yeast, founded in 1986, is a listed high-tech yeast company in China.
• It is one of the largest global yeast and yeast extract manufacturers with 12 factories in China and Egypt up to now.
1.2 Global network
12Factories 4Yeast extract plants
12Technical centers 140Countries
1989 2000 20031986
Project started Public company No.1 in Asia
First facilities First yeast extract facilities
Global supplier
Today2008
1.3 History
The fastest growing company in global yeast industry
• Angel yeast is estimated to grow at a CAGR of 20% 2011-2012, while the global yeast market’s CAGR is to be 8.6% (Foodnavigator.com, 2012-04-05).
CAGR 2011-2012
Main business
• Bakery• Yeast extract-savory• Yeast extract-fermentation• Beverage & ethanol• Human health• Enzymes• Animal nutrition
1.4 Main business
1.5 Angel’s Value Chain
MicroorganismMicroorganism
Industrial fermentationIndustrial fermentation
ProductProduct
BrewingBrewing FeedFeed PharmPharmEnvironmentEnvironment EnergyEnergyAgricultureAgricultureFoodFood ChemicalChemical
LabLab
Wine yeastWine yeast
EnzymeYeast extractYeast cell wall
Feed yeast
EnzymeYeast extractYeast cell wall
Feed yeast
EnzymeActive yeastYeast extractInactive yeast
EnzymeActive yeastYeast extractInactive yeast
Yeast extractYeast extract
Alcohol yeastAlcohol yeastYeast extractBio-fertilizer
Yeast extractBio-fertilizer
BakeryEnzyme
SeasoningInactive yeastYeast extract
BakeryEnzyme
SeasoningInactive yeastYeast extract
EnzymeEnzyme
Yeast extractYeast extract
Yeast extractInactive yeast
Technical supportExperiment
Yeast extractInactive yeast
Technical supportExperiment
PackageTrading
PackageTrading
Our global partners
1.6 Employees>• 1331 employees, 70% of which are professional people• Over 100 researchers work together with universities.• 7 international experts employed as senior technical consultants.
Rosenberg (Netherlands) He Chongyue
(Taiwan, China)
Yao Juan Li Xiao Kuang Jinbao Li Pei Li Zhijun
Tilak (U.S.)Jan Hille ((Netherlands)
1.7 Advantages
1.7.1 Strong production output >1.7.2 Professional production process>1.7.3 R&D strength >1.7.4 Quality, safety and consistency >1.7.5 Certification>1.7.6 Environment protection >1.7.7 Global professional service >
1.7.1 Strong production output >
Yichang Plant Yili Plant
Chongzuo Plant Binzhou Plant
Total output of
Yeast is 120.000 tons, Yeast Extract is 46.000 tons.
1.7.2 Professional production process
1.7.3 R&D strength >• 4 sub-headquarters with technical centers
• National yeast technology research and promotion center
• Post-doctoral scientific research workstation
• R & D team led by PhDs and experts
• National recognized laboratory CNAS
• Basic research and Application research laboratory
• Own the first-class test device and pilot plant.
1.7.4 Quality, safety and consistency >
• EU standard design production line
• Stable and consistent quality applies to all production plants
• Distributed control system
• Siemens PCS7 process control system
• Whole-course CIP cleaning
• SAP-ERP Management flat
1.7.5 Certifications >
BRC SEDEX
ISO22000
HALAL KOSHER(OU)
ISO14001ISO9001CNAS
1.7.6 Environment protection >
• First class environment protection facilities in each plant
• The high-concentration organic waste water was made into bio-organic fertilizer.
1.7.7 Global professional service >
• We do not only provide the best quality products, we also provide technical solutions for all our serving industries
• Set up global baking centers• Over 200 application engineers
2. Angel Yeast Extracts – Good nutrient-sources for microoganisms
2.1 What’s yeast extract >2.2 Why choose yeast extract for fermentation industry > 2.3 Range of Angel’s typical products >
2.1 What’s yeast extract?
Yeast extract is a kind of :
• 100% animal free natural ingredient that is made from yeast.
• High performance and stable sources of nutrients for microorganisms
• Clean and safe raw materials for industrial fermentation and culture media
Yeast extract is a kind of :
• 100% animal free natural ingredient that is made from yeast.
• High performance and stable sources of nutrients for microorganisms
• Clean and safe raw materials for industrial fermentation and culture media
Process of yeast extract production
Process of yeast extract production
2.1.1 Molecular weight distribution
2.1.2 Profile of Amino acids
Asp Thr Ser Glu Gly Ala Val Met Ile Leu Tyr Phe Lys His Arg Pro0
1
2
3
4
5
6
7
Con
cent
rati
on (
%)
Amino acids
A0128 A0316 A0418OX3098OX6919
2.1.3 Profile of free Amino acids and Total amino acids
Free Amino acids / Total Amino acids ×100Free Amino acids / Total Amino acids ×100
2.1.4 Trace elements
Name of elements Concentration ( mg/kg)Ca 246Mg 149Zn 86Fe 33Cr 1.26
Mn 0.08K 9172
Na 2022As 0.03Se 0.002Pb 0.19
2.1.5 Vitamins
Name of vitamins Concentration ( mg/kg )
Vitamin B1 2.7
Vitamin B2 56.9
Niacin 336
Vitamin B6 17.6
Pantothenic acid 29
Free D-Biotin 500 ( μg/100g )
2.1.6 Nucleic acid and Nucleotides
0.0
0.5
1.0
1.5
2.0
2.5
3.0
Con
cent
rati
on (
%)
RNA
A0128 A0316 A0418 OX3098 OX6919
UMP CMP GMP IMP AMP0.00
0.02
0.04
0.06
0.08
0.10
0.12
Con
cent
rati
on (
mg/
kg)
UMP, CMP, GMP,IMP and AMP
A0128 A0316 A0418 OX3098 OX6919
2.1.7 Functionality of yeast extract
• Increase biomass • Improve the accumulation of metabolites
2.1.8 Stability of yeast extract
Two key indicators: Total nitrogen and Amino nitrogen, For example: Stability of Yeast extract FM902
2.2 Why we choose yeast extract for fermentation industry? • Clean and Safe - Clean and safe natural material for culture media and industrial fermentation - Animal free, no risks of illness - GMO free, Non allergen - Kosher and Halal certified.
• Stable quality - Produced with strict quality control system. - Large industry scale production - No quality change in different seasons
• High performance - Advanced production technology
Beside yeast extract, Angel also produce yeast peptone and inactive yeast for fermentation industry
2.3 Range of Angel’s typical productsMicrofiltered yeast extract FM888 Powder Lab, Biopharmaceuticals
Nutrients-enriched yeast extractFM803
FM505
Powder
PowderProbiotics, Starter culture
Yeast peptoneFP101
LP101
Powder
PasteProbiotics, culture media
Standard yeast extract
FM808
FM902
LM800
LD00
Powder
Powder
Paste
Liquid
Industrial fermentation
Autolyzed & Inactive yeastFM801
YP101
Powder
PowderAntibiotics, APIs
Economical yeast extractFM701
LM815
Powder
PasteAgriculture and environment
Products
FM888 FP101
Which are the most popular products in lab research?
Which are the most popular products in lab research?
Angel Microfiltered yeast extract FM888
• Features and Advantages - High solubility- Easily assimilated by microorganisms and cells- Rich in peptides and growth factors- Low impurities
• Application- Lab research Dehydrated culture media, Cell culture, Diagnostic media- High value-added industrial fermentation Insulins, Hormones, Hyaluronic acids
Angel yeast peptone FP101
• Features and Advantages - Good replacement of Animal origin peptone- Could be combined with yeast extract- Rich in peptides
• Application- Lab research Microorganisms culture, small scale fermentation- Industrial fermentation Probiotics, Enzymes, Hormones, Organic acids
Products
FM808
FM902
LD00
LM800
Which are the most popular products in industrial
fermentation?
Which are the most popular products in industrial
fermentation?
Angel standard yeast extract FM808
• Features and Advantages - Good solubility- High nitrogen level- Rich in peptides- Light color
• Application- Lab research Dehydrated culture media- Industrial fermentation Insulins, Hormones, Hyaluronic acids, Probiotics
Angel standard yeast extract FM902
• Features and Advantages - Total soluble in water- High nitrogen level- Cost effective- High consistency
• Application- Industrial fermentation
For >60 types of fermentation-based products
Angel standard yeast extract FM902
Angel standard yeast extract FM902
Angel standard yeast extract FM902
Angel standard yeast extract FM902
FM902FM902
EnvironmentEnvironment
AgricultureAgriculture
Industrial fermentationIndustrial fermentation
Amino acidEnzymeHormoneOrganic acidProbioticNucleotides / NucleosidesPoly saccharideBiological preservativesAntibiotic
Amino acidEnzymeHormoneOrganic acidProbioticNucleotides / NucleosidesPoly saccharideBiological preservativesAntibiotic
MashroomRhizobiaBiological baitEffective MicroorganismsBio-fertilizer
MashroomRhizobiaBiological baitEffective MicroorganismsBio-fertilizer
Waste water treatment
Environmental microorganisms
Waste water treatment
Environmental microorganisms
Angel standard yeast extract LM800 and LD00
• Features and Advantages - Paste and Liquid form of yeast extract FM902- Rich in peptides- Cost effective- High consistency
• Application- Industrial fermentation
For >60 types of fermentation-based products
Better yeast extract perform better
solubilityDepends
Good solubility means readily assimilated nutrients,
but it depends on specific needs.
Better yeast extract perform lighter
colorDepends
Some fermentation need light color
Better yeast extract perform higher
amino nitrogenDepends
Higher amino nitrogen product is suitable for seed
culture and probiotics, not all the fermentation
Yeast extract should contain low level
active microbesTrue
High level active microbes cause contamination
Less biomass and productivity means
poor quality of yeast extractDepends
40% of this situation means unsuitable process,
condition or culture media formula
Baker’s yeast extract is better than
Brewer’s yeast extractTrue
Brewer’s yeast extract is not stable which come from
spent brewer’s yeast
Baker’s yeast extract must get
Kosher/Halal certificatesTrue
Clean and safe
Yeast extract contain rich protein Depends It depends on the customers specific needs
How to judge the quality?
3. Application of Angel Yeast Extract
3.1 Summary of Angel yeast extract application >3.2 Some experiments of Angel yeast extract >
3.1 Summary of Angel yeast extract application
Biomass products industry - Starter cultures and probiotics for human food products - Cultivation of mashroom and macrofungi Recommendation: FM808, FM803, FM505, FP101
Culture media industry and lab reagent - Bench scale research - Dehydrated culture media, Diagnostics, Cell cuture Recommendation: FM888, FM808
Bioactive molecules and chemicals industry - Amino acids and peptides - Organic acids, Vitamins, Nucleotides, Enzymes, Alcohol - Vitamins, Antibiotics, Hormones, Biological preservatives - Other types of large scale fermentation Recommendation: FM808, FM902, LM800, LD00, YP101
Agricultural and environmental industry - Sewage treatment, Bio-pesticide, Bio-plastic - Probiotics for agriculture and environment Recommendation: FM902, FM801, FM701, LM815, LP101
3.2 Some experiments of Angel yeast extract
3.2.1 Morphology of 4 types of typical microorganisms >3.2.2 Some experiments of Angel yeast extract >
3.2.1 Morphology of 4 types of typical microorganisms after using yeast extract and other type of organic nitrogen
A B
AA
A
BB
B
“A”—Another type of organic nitrogen “B”—Angel yeast extract
Streptomyces lincolnensis Lactobacillus bulgaricus
Bacillus subtilis Acetobacterium
3.2.2 Angel standard yeast extract FM808 for Lactobacillus thermophilus growth
0
20
40
60
80
100
12 16 20 24 28 32 36 40 44 48
安琪酵母浸粉 FM888
国外某试剂级酵母浸粉
0
2
4
6
8
10
12
14
16
18
12 16 20 24 28 32 36 40 44 48
安琪酵母浸粉 FM888
国外某试剂级酵母浸粉
Strain: Lactobasillus thermophilus
Medium: Yeast Extract FM808 0.3% , Glucose 2%, etc. pH: 6.5
Result: Yeast extract FM808 could improve the growth of Lactobacillus thermophilus well
Angel yeast extract FM808Angel yeast extract FM808
ControlControl
Angel yeast extract FM808Angel yeast extract FM808
ControlControl
3.2.3 Angel standard yeast extract FM902 for the growth of E.coli and Absidia coerulea
The growth curve of E.coli, comparison of FM902 and Brewer’s yeast extract
The conversion rate of HC(from RSA), Absidia coerulea, yeast extract FM902
3.2.4 Angel nutrients enriched yeast extract FM803 for probiotics
0
0.4
0.8
1.2
1.6
2
0 3 6 9 12 15 18 21 24 27 30
时间(h)
OD
600
FM803
国外某试剂级酵母浸粉
0
0.2
0.4
0.6
0.8
1
1.2
0 3 6 9 12 15 18 21 24 27 30时间(h)
OD
600
FM803
国外某试剂级酵母浸粉
Strain: Lactobacillus rhamnosusMedium: MRS
Strain: Lactobacillus helveticusMedium: MRS
Lactobacillus needs complicated nutritional condition for its growth and acid production. Yeast extract is rich in amino acids, small peptides and growth factors which Lactobacillus needs. Yeast extract FM803 is a kind of free amino acids enriched product and could improve the growth of Lactobacillus.
Angel yeast extract FM803Angel yeast extract FM803
ControlControl
Angel yeast extract FM803Angel yeast extract FM803
ControlControl
Fig.1 The effect on L-Tryptophan when different ratio of yeast extracts were used
Fermentation culture medium(%): yeast extract FM902, glucose 2 %, ammonium sulfate 0.4%, potassium dihydrogen phosphate 0.15%, etc.
Conclusion:FM902 is rich in small peptides and free amino acids, and it also contains appropriate polypeptides, vitamins and trace elements. Thus FM902 is suitable for the fermentation of L-Tryptophan, 2% is a proper level of yeast extract ratio.
3.2.5 Angel standard yeast extract FM902For L-Tryptophan
Department of Microbial Nutrition Research
• The research and development of new products
• The optimization of culture media formula
• The suggestion of fermentation process
• Development of tailor-made products
Technical support
Test and scale up platform
HPLC Atomic Absorption Spectrometer
GC Amino acid analyzer
GC-MS Powder Instrument Drawing Instrument Ultrasonic Processors
Pilot Plant Platform for fermentation control The membrane separation system Spray drying system
Any question, please feel free to contact me:
Johnny Yin
Angel Yeast Co.,Ltd.
Address: 168 Cheng Dong Avenue, Yichang, Hubei, China. 443003.
Email: [email protected];
Contact information
THANK YOU!