Potpourri

FINAL JEOPARDY!500

400300200100

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Send in the clones Gel - e

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100 Cloning How could cloned stem cells or

diseased animals advance medicine?

Less time, energy and money in the long run to make exact copies of the diseased animal compared to inserting the diseased gene.Numerous stem cells could advance the possibility of creating organs that could replace unhealthy ones.

200

Discuss a positive and negative of cloning

endangered or extinct species

Positive: Maintain biodiversityNegative: Doesn’t address loss of habitat

300Explain how artificial twinning is different

than somatic cell nucleus transfer (SCNT).

Artificial twinning separates an embryo in the very early of stages of development to created two identical individuals. SCNT transfers the DNA from a body cell and transfers it to an enucleated egg cell. Both processes uses a surrogate mother.

400

What is a cloning vector?

It carries the donor DNA to a host cell where it can be replicated many times. For instance bacterial plasmid with a donor gene for insulin can be inserted into a bacteria.

500

If the surrogate mouse has brown hair

and the donor gene produces white hair, what color hair will the clone

have? Why?

White hair.

100

1 2 3 4 5 6

In this diagram which end, the top or bottom,

would be negatively

charged?Why?

Bottom

200

1 2 3 4

Sample 1 is from the mother and 2 is from the child. Samples 3

& 4 are from potential fathers. Who is most likely to be the father

and why?

4

300Describe the mechanics behind the way a gel electrophoresis works.

Opposite charges are on the ends of the gel. DNA, a negatively charged molecule, is pushed away from the negative pole it was placed by. The lighter DNA fragments will travel the farthest in the shortest time

400List three ways that DNA

fingerprinting can be used.

Paternity testing, tracing human origins, proving innocence or guilt.

500

What is polymerase chain reaction?

A method to make many copies of DNA. It is associated with degraded or damaged DNA.

Potpourri for 100

What are the positives and negatives of GMOs that are

used in agriculture?

Pro: less pesticide use, kills target species, creates more nutritious food, Cons: may contain unknown allergens, unsure of harm, disrupts natural selection

Potpourri for 200Why are stem cells used in

studying treatments for diseases?

They are undifferentiated cells that can be stimulated to develop into specific types of cells.

Potpourri for 300Why is DNA fingerprinting more accurate than blood typing for

paternity testing?

A child may not have the same blood type as either parent if it inherits two recessive alleles.

Potpourri for 400

Why is non-coding DNA used in DNA identification?

It is the .09% of DNA that signifies uniqueness among humans.

Potpourri for 500How are sticky ends created?

Restriction enzymes cut specific nucleotide sequences that leave the DNA with jagged ends. .

How are restrictions enzymes beneficial to

bacteria? They provide a means of defense against invaders.