α-ketoglutarate signals N-deprivation in an

association between Nostoc punctiforme and Anthocerus

punctatus

Jeff Elhai

IntroductionBackgroundThe world population is the total number of living humans on Earth. As of today, it is estimated to number 7.055 billion by the United States Census Bureau (USCB).[1] The USCB estimates that the world population exceeded 7 billion on March 12, 2012.[2]

Green revolution plants are able to make use of more N fertilizer.

With advances in molecular genetics, the mutant genes responsible for Arabidopsis thaliana genes (GA 20-oxidase,[17] ga1,[18] ga1-3[19]), wheat reduced-height genes (Rht)[20] and a rice semidwarf gene (sd1)[21] were cloned.

Norman Borlaug is considered the Father of the Green Revolution.

To feed an evergrowing population, it is necessary to increase the availability of food. Green Revolution refers to a series of research, development, and technology transfer initiatives, occurring between the 1940s and the late 1970s, that increased agriculture production around the world, beginning most markedly in the late 1960s.

IntroductionAdditional Background

Nostoc punctiforme forms symbioses with many plants. It is a heterocystous cyanobacterium that fixes nitrogen in semiregularly spaced cells.

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An experiment

Nostoc passes fixed nitrogen to the plant

Materials and Methods

GltI was amplified from the genomic DNA by PCR and subcloned into pRSETB (Invitrogen). Truncation combinations were amplified with Phusion polymerase(NEB) and purified with 96-well PCR cleanup cartridges (Qiagen).

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Results

These are the kind of results I expect to get.

More results

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are needed to see this picture.

Discussion

World hunger is one of the gravest threats facing our world today. Every year 15 million children die of hunger. If this experiment helps even a small fraction of them it will be worth it.

References

Any Questions?