Curriculum Vitae

Jane McHowat

Department of Pathology Saint Louis University School of Medicine

Doisy Research Center 1100 S. Grand Blvd. St. Louis, MO 63104

Tel: (314) 977-9295 Fax: (314) 977-8499 Email: mchowaj@slu.edu

EDUCATION: 1980 - 1983 De Montfort University

B. Sc. (First Class Honors) Pharmacy 1983 - 1984 Leicestershire Area Health Authority

Pharmacy Pre-Registration Training Year 1984 - 1988 University of Bath

Ph. D. Pharmacology Cardiovascular Control by Central Beta-Adrenoceptors in the Rat

ACADEMIC POSITIONS: 1987 - 1988 Leicester General Hospital

Resident Pharmacist 1988 - 1989 Lewisham Hospital

Pharmacist Grade D Residency & Research 1989 - 1993 Washington University School of Medicine

Department of Cardiology, Postdoctoral Research Fellow 1993 - 1997 University of Arkansas for Medical Sciences

Department of Pathology, Research Assistant Professor 1997-2002 Saint Louis University School of Medicine

Department of Pathology, Assistant Professor 2002-2007 Saint Louis University School of Medicine

Department of Pathology, Associate Professor 2007-Present Saint Louis University School of Medicine

Department of Pathology, Professor SOCIETY MEMBERSHIPS: American Heart Association American Physiological Society

PUBLICATIONS: Manuscripts, Reviews and Book Chapters: 1. Wu, J., McHowat, J., Saffitz, J.E., Yamada, K.A., Corr, P.B. Inhibition of gap junctional conductance by long-chain acylcarnitines and their preferential accumulation in junctional sarcolemma during hypoxia. Circ. Res. 1993; 72, 879-889 PMID: 84438742. Yan, G-X., Yamada, K.A., Kleber, A.G., McHowat, J., Corr, P.B. Dissociation between cellular K+ loss, reduction in repolarization time and tissue ATP levels during myocardial hypoxia and ischemia. Circ. Res. 1993; 72, 560-570 PMID: 8431084 3. McHowat, J., Yamada, K.A., Saffitz, J.E., Corr, P.B. Subcellular distribution of endogenous long chain acylcarnitines during hypoxia in adult canine myocytes. Cardiovasc. Res. 1993; 27, 1237-1243 PMID: 8252583 4. McHowat, J., Corr, P.B. Thrombin-induced release of lysophosphatidylcholine from endothelial cells. J. Biol. Chem. 1993; 268, 15605-15610 PMID: 8393449 5. McHowat, J., Yamada, K.A., Wu, J., Yan, G-X., Corr, P.B. Recent insights pertaining to sarcolemmal phospholipid alterations underlying arrhythmogenesis in the ischemic heart. J. Cardiovasc. Electrophys. 1993; 4, 288-310 PMID: 8269301 6. Yamada, K.A., McHowat, J., Yan, G-X., Donahue, K., Peirick, J., Kleber, A.G., Corr, P.B. Cellular uncoupling induced by accumulation of long-chain acylcarnitine during ischemia. Circ. Res. 1994; 74, 83-95 PMID: 8261598 7. McHowat, J., Corr, P.B. Biochemical membrane mechanisms underlying arrhythmias during myocardial ischemia and their role in sudden cardiac death. In: Sudden Cardiac Death, Akhtar, Myerburg & Ruskin eds., Williams & Wilkins, Philadelphia, 1994; 82-101 8. Ido, Y., McHowat, J., Chang, K.C., Arrigoni-Martelli, E., Orfalian, Z., Kilo, C., Corr, P.B., Williamson, J.R. Neural dysfunction and metabolic imbalances in diabetic rats: Prevention by acteyl-l-carnitine, Diabetes 1994; 43, 1469-1477 PMID: 7958501 9. Corr, P.B., Yamada, K.A., Creer, M.H., Wu, J., McHowat, J., Yan, G-X. Amphipathic lipid metabolites and arrythmias during ischemia. In: Cardiac Electrophysiology, From Cell To Bedside, Zipes & Jalife eds., Saunders, Philadelphia, 1994; 182-203 10. Park, T.H., McHowat, J., Wolf, R.A., Corr, P.B. Increased lysophosphatidylcholine content induced by thrombin receptor stimulation in adult cardiac ventricular myocytes, Cardiovasc. Res. 1994; 28, 1263-1268 PMID: 7954631 11. Corr, P.B., McHowat, J., Yan, G-X., Yamada, K.A. Lipid derived amphiphiles and their contribution to arrhythmogenesis during ischemia. In: Physiology and Pathophysiology of the Heart, Sperelakis ed., Kluwer Academic, 1995, 527-545 12. McHowat, J., Creer, M.H. High-molecular weight intracellular calcium-independent phospholipase A2 (cPLA2), in: Phospholipase A2 in Clinical Inflammation: Endogenous Regulation and Pathophysiologic Actions. CRC Series on Pharmacology and Toxicology: Basic and Clinical Aspects, K.B. Glaser and P. Vadas, editors, 1995; 75-92 13. McHowat, J., Jones, J.H., Creer, M.H. Quantitation of individual phospholipid molecular species by UV absorption measurements. J Lipid Res. 1996; 37, 2450-2460 PMID: 8978496 14. McHowat, J., Liu, S. Interleukin-1β stimulates phospholipase A2 activity in adult rat ventricular myocytes, Am. J. Physiol. 1997; 272, C450-C456 PMID: 9124287 15. McHowat, J., Jones, J.H., Creer, M.H. A gradient-elution, reverse phase high performance liquid chromatographic technique for the separation of individual phospholipid molecular species, J. Chromatog. B. 1997; 702, 21-32 PMID: 9449552 16. McHowat, J., Creer, M.H. Lysophosphatidylcholine accumulation in cardiomyocytes requires thrombin activation of Ca2+-independent PLA2, Am. J. Physiol. 1997; 272, H1972-H1980 PMID: 9139985

17. McHowat, J., Creer, M.H. Thrombin activates a membrane-associated calcium-independent PLA2 in ventricular myocytes, Am. J. Physiol. 1998; 274, C447-C454 PMID: 9486135 18. McHowat, J., Liu, S., Creer, M.H. Selective hydrolysis of plasmalogen phospholipids by Ca2+-independent PLA2 in hypoxic ventricular myocytes. Am. J. Physiol. 1998; 274, C1727-C1737 PMID: 961113919. Liu, S., McHowat, J. Stimulation of different phospholipases A2 by tumor necrosis factor-α and interleukin-1β in adult ventricular myocytes. Am. J. Physiol. 1998; 275, H1462-H1472 PMID: 9746498 20. McHowat, J., Creer, M.H. Calcium-independent phospholipase A2 in isolated rabbit ventricular myocytes. Lipids, 1998; 33, 1203-1212 PMID: 9930406 21. Creer, M.H., McHowat, J. Selective hydrolysis of plasmalogens in endothelial cells following thrombin stimulation. Am. J. Physiol. 1998; 275, C1498-C1507 PMID: 9843711 22. McHowat, J., Creer, M.H. Selective plasmalogen substrate utilization by thrombin-stimulated Ca2+- independent PLA2 in cardiomyocytes. Am. J. Physiol.2000; 278, H1933-H1940 PMID: 10843891 23. McHowat, J., Creer, M.H., Hicks, K.K., Jones, J.H., McCrory, R.D., Kennedy, R.H. Induction of Ca2+-independent PLA2 and conservation of plasmalogen polyunsaturated fatty acids in diabetic heart. Am. J. Physiol. 2000; 279, E25-E32 PMID: 108933319 24. Cummings, B.S., McHowat, J., Schnellmann, R.G. Phospholipase A2s in cell injury and death. J. Pharmacol. Exp. Ther.2000; 294, 793-799 PMID: 10945826 25. McHowat, J., Creer, M.H. Biologic and mechanistic diversity of mammalian phospholipase A2 (PLA2). In: Recent Research Developments in Lipids, 2000; 4, 13-24 26. McHowat, J., Tappia, P.S., Liu, S-Y, McCrory, R.D., Panagia, V. Redistribution and abnormal activity of cardiac phospholipase A2 isoenzymes in post-infarct congestive heart failure. Am. J. Physiol., 2001; 280, C573-C580 27. McHowat, J., Creer, M.H., Rickard, A. Stimulation of protease-activated receptors on RT4 cells mediates arachidonic acid release via Ca2+-independent PLA2 (iPLA2). J. Urol., 2001; 165, 2063-2067 PMID: 11371929 28. McHowat, J., Swift, L.M., Arutunyan, A., Sarvazyan, N. Clinical concentrations of doxorubicin inhibit activity of myocardial membrane-associated calcium-independent phospholipase A2. Cancer Res., 2001; 61, 4024-4029 PMID: 11358821 29. McHowat, J., Kell, P.J., O’Neill, H.B., Creer, M.H. Endothelial cell PAF synthesis following thrombin stimulation utilizes Ca2+-independent phospholipase A2. Biochemistry, 2001;40, 14921-14931 PMID: 11732912 30. McHowat, J., Creer, M.H. Comparative roles of phospholipase A2 isoforms in cardiovascular pathophysiology. Cardiovasc. Toxicol. 2001; 1, 253-265 PMID: 12189273 31. McHowat, J., Swift, L.M., Sarvazyan, N. Oxidant-induced inhibition of myocardial calcium-independent phospholipase A2. Cardiovasc. Toxicol. 2001; 1, 309-316 PMID: 12213969 32. McHowat, J., Creer, M.H. Myocardial phospholipase A2 (PLA2) exhibits unique catalytic features optimized for regulation of cardiovascular function. In: Recent Research Developments in Lipids, 2002; 2, 19-30 33. Cummings, B.S., McHowat, J., Schnellmann, R.G. Role of an endoplasmic reticulum Ca2+-independent phospholipase A2 in oxidant-induced renal cell death. Am. J. Physiol., 2002; 283, F492-F498 PMID: 12167600 34. Maggi, L.B., Moran, J.M., Scarim, A.L., Ford, D.A., Yoon, J-W., McHowat, J., Buller, M.L., Corbett, J.A. Novel role for calcium-independent Phospholipase A2 in the macrophage antiviral response of inducible nitric-oxide synthase expression. J. Biol. Chem. 2002; 277, 38449-38455 PMID: 12167650 35. Rickard, A., McHowat, J. Production of phospholipid metabolites following cleavage of protease-activated receptors on human urothelial cells. Am. J. Physiol. 2002; 283, F944-F951 PMID: 12372769

36. Steer, S.A., Wirsig, K.C., Creer, M.H., Ford, D.A., McHowat, J. Regulation of membrane-associated iPLA2 activity by a novel PKC in ventricular myocytes. Am. J. Physiol. 2002; 283, C1621-C1626 PMID: 12419708 37. Swift, L., McHowat, J., Sarvazyan, N. Inhibition of membrane-associated calcium-independent phospholipase A2 as a potential culprit of anthracycline cardiotoxicity. Cancer Res. 2003; 63, 5992-5998 PMID: 14522926 38. Liu, S.J., Creer, M.H., Kennedy, R.H., McHowat, J. Alterations in Ca2+ cycling by lysoplasmenylcholine in adult rabbit ventricular myocytes. Am. J. Physiol. 2003; 284, C826-C838 PMID: 12456398 39. Thukkani, A.K., McHowat, J., Hsu, F.-F., Hazen, S.L., Ford, D.A. Identification of α-chloro fatty aldehydes and unsaturated lysophosphatidylcholine in human atherosclerotic lesions: P-selectin activation by unsaturated lysophosphatidylcholine. Circulation 2003; 108, 3128-3133 PMID; 14638540 40. McHowat, J. The cardiovascular consequences of calcium-independent phospholipase A2 activation. In: Recent Research Developments in Physiology, 2003; 1, 521-538 41. Kell, P.J., Creer, M.H., Crown, K.N., Wirsig, K., McHowat, J. Inhibition of platelet-activating factor (PAF) acetylhydrolase by methyl arachidonyl fluorophosphonate potentiates PAF synthesis in thrombin-stimulated human coronary artery endothelial cells. J. Pharmacol. Exp. Ther. 2003; 307, 1163-1170 PMID: 14560038 42. Cummings, B.S., McHowat, J., Schnellmann, R.G. Role of an endoplasmic reticulum Ca2+- independent phospholipase A2 in cisplatin- induced renal cell apoptosis. J Pharmacol Exp Ther. 2004; 308, 921-928 PMID: 14634037 43. Ray, R.B., Basu, A., Steele, R., Beyene, A., McHowat, J., Meyer, K., Ghosh, A.K., Ray, R. Ebola virus glycoprotein mediated anoikis of primary human cardiac microvascular endothelial cells. Virology, 2004; 321, 181-188 PMID: 15051379 44. McHowat, J., Creer, M.H. Catalytic features, regulation and function of myocardial phospholipase A2. Current Med. Chem. 2004; 2, 209-218 PMID: 15320787 45. Meyer, M., McHowat, J. The role of platelet-activating factor in the adherence of circulating cells to the endothelium. Recent Research Developments in Physiology 2004; 2, 129-147 46. Cummings, B.S., Gelasco, A.K., Kinsey, G.R., McHowat, J., Schnellmann, R.G. Inactivation of endoplasmic reticulum bound Ca2+- independent phospholipase A2 in renal cells during oxidative stress. J. Am. Soc. Nephrol. 2004;15, 1441-51. PMID: 15153555 47. McHowat, J., Swift, L., Crown, K.N., Sarvazyan, N. Changes in phospholipid content and myocardial calcium-independent phospholipase A2 activity during chronic anthracycline administration. J Pharmacol. Exp. Ther. 2004; 311, 736-741 PMID: 15295018 49. Meyer, M.C., Kell, P.J., Creer, M.H., McHowat, J. Calcium-independent Phospholipase A2 is regulated by a novel protein kinase C in human coronary artery endothelial cells. Am. J. Physiol. 2005; 288, C475-C482 PMID: 15643055 50. Kinsey, G.R., Cummings, B.S., Beckett, C.S., Saavedra, G, Zhang, W., McHowat, J., Schnellman, R.G. Identification and distribution of endoplasmic reticulum iPLA2 Biochem. Biophys. Res. Comm. 2005; 327, 287-293 PMID: 15629460 51. Rickard, A., Portell, C., Kell, P.J., Vinson, S.M., McHowat J. Protease activated receptor stimulation activates a calcium-independent phospholipase A2 in bladder microvascular endothelial cells. Am. J. Physiol. 2005; 288, F714-F721 PMID: 15561975 52. Meyer, M.C., Rastogi, P., Beckett, C.S., McHowat, J. Phospholipase A2 inhibitors as potential anti-inflammatory agents. Current Pharmaceutical Design 2005: 11, 1301-1312 PMID: 15853686 53. Patrick, C.B., Goldstein, D.S., McHowat, J., Rosenberger, T.A., Rapoport, S.I., Murphy, E.J. Arachidonic acid incorporation and turnover is decreased in sympathetically denervated rat heart. Am. J. Physiol. 2005; 288, H2611-H2619 PMID: 15681700 54. Moran, J.M., Buller, R.M., McHowat, J., Turk, J., Wohltmann, M., Gross, R.W., Corbett, J.A. Genetic and pharmacologic evidence that iPLA2β regulates virus-induced iNOS expression by

macrophages J. Biol. Chem., 2005; 280, 28162 - 28168 PMID: 15946940 55. Vinson, S.M., Rickard, A., Ryerse, J.S., McHowat, J. Neutrophil adherence to bladder microvascular endothelial cells following platelet-activating factor acetylhydrolase inhibition. J. Pharm. Exp. Ther. 2005; 314, 1241-1247 PMID: 15937147 56. Meyer, M.C., Creer, M.H.., McHowat, J. A potential role for mast cell tryptase in the recruitment of inflammatory cells to the endothelium. Am. J. Physiol. 2005; 289, C1485-C1491 PMID: 16079184 57. Beckett, C.S., Pennington, K., McHowat, J. Activation of MAP kinases in thrombin-stimulated ventricular myocytes is dependent upon calcium-independent phospholipase A2. Am. J. Physiol., 2006; 290, C1350-C1354 PMID: 16338969 58. Portell, C., Rickard, A., Vinson, S.M., McHowat, J. Prostacyclin production in tryptase and thrombin stimulated human bladder endothelial cells: Effect of pretreatment with phospholipase A2 and cyclooxygenase inhibitors. J. Urol. 2006; 176, 1661-1665 PMID: 16952710 59. Rastogi, P., Mchowat, J. Eicosanoids as Therapeutic Targets in Asthma. Letters in Drug Design and Discovery. 2006; 3, 668-674 60. Rastogi, P., Beckett, C.S., McHowat, J. Prostaglandin production in human coronary artery endothelial cells is modulated differentially by selective phospholipase A2 inhibitors. Prostaglandins, Leukotrienes and Essential Fatty Acids. 2007; 76, 205-212 PMID: 17374479 61. Meyer, M.C., McHowat, J. Calcium-independent phospholipase A2-catalyzed plasmalogen hydrolysis in hypoxic human coronary artery endothelial cells. Am J Physiol Cell Physiol. 2007; 292, 251-258 PMID: 16943248 62. Beckett, C.S., Kell, P.J., Creer, M.H., McHowat, J. Phospholipase A2-catalyzed hydrolysis of plasmalogen phospholipids in thrombin-stimulated platelets. Thromb. Res. 2007; 120, 259-268 PMID: 17055038 PMCID: PMC2204082 63. Kinsey, G.R., McHowat, J., Schnellmann, R.G. Identification of calcium-independent phospholipase A2γ in mitochondria and its role in mitochondrial oxidative stress. Am. J. Physiol. 2007; 292, 853-860 PMID: 17047165 64. White, M.C., McHowat, J. The therapeutic potential of phospholipase A2 inhibitors in cardiovascular disease. Cardiovascular & Hematological Agents in Medicinal Chemistry 2007; 5, 91-95 PMID: 17266551 65. White, M.C., McHowat, J. Protease activation of calcium-independent phospholipase A2 leads to neutrophil recruitment to coronary artery endothelial cells. Thromb. Res. 2007; 120, 597-605 PMC2170458 66. Kinsey, G.R., McHowat, J., Patrick, K.S., Schnellmann, R.G. Role of Ca2+-independent phospholipase A2γ in Ca2+-induced mitochondrial permeability transition. J. Pharmacol. Exp. Ther. 2007; 321, 707-15 PMID: 17312185 68. Swift, L., McHowat, J., Sarvazyan, N. Anthracycline-induced phospholipase A2 inhibition. Cardiovasc. Toxicol., 2007; 7, 86-91 17652810 69. White, M.C., Rastogi, P., McHowat, J. Lysoplasmenylcholine increases neutrophil adherence to human coronary artery endothelial cells. Am. J. Physiol. 2007; 293, C1467-C1471 PMID: 17728394 70. Messner, M.C., Albert, C.J., Ford, D.A., McHowat, J. Identification of lysophosphatidylcholine-chlorohydrin in human atherosclerotic lesions. Lipids. 2008; 43, 243-249. PMC2741177 71. Rastogi, P., Rickard, A., Dorokhov, N., Klumpp, D.J., McHowat, J. Loss of prostaglandin E2 release from urothelial cells isolated from an interstitial cystitis patient, Am. J. Physiol. 2008; 294, F1129-F1135 NIHMSID: 180212 72. Kinsey, G. R., Blum, J.L., Covington, M.D., Cummings, B.S., McHowat, J., Schnellmann, R.G. Decreased iPLA2γ expression induces lipid peroxidation, cell death, and sensitizes cells to oxidant-induced apoptosis. J. Lipid Res. 2008; 49, 1477-1487 PMC2431104 73. Rastogi, P., White, M.C., Rickard, A., McHowat, J. Potential mechanism for recruitment and migration of CD133 positive cells to areas of vascular inflammation. Thromb. Res. 2008; 123, 258-

266 PMC2678553 74. Beckett, C.S., McHowat, J. Calcium-independent phospholipase A2 in rabbit ventricular myocytes. Lipids. 2008; 43, 775-782 PMC2831212 75. Rickard, A., Dorokhov, N., Ryerse, J., Klumpp, D., McHowat J. Characterization of Tight Junction Proteins in Cultured Human Urothelial Cells, In Vitro Cell. Dev. Biol. Anim. 2008; 44, 261-267 NIHMSID: 180380 76. Rastogi, P., Young, D.M., McHowat, J. Tryptase activates calcium independent phospholipase A2, releasing PGE2 in airway epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 2008; 295, L925 - L932 PMC2584891 77. Rastogi, P., McHowat, J. Inhibition of calcium independent phospholipase A2 prevents inflammatory mediator production in pulmonary microvascular endothelium. Resp. Physiol. Neurobiol. 2009; 165, 167-174 PMC2845306 78. Sharma, J., Rastogi, P., Creer, M.H., McHowat, J. Polymorphonuclear leukocytes isolated from umbilical cord blood are a useful research tool to study adherence to cell monolayers, J. Immunol. Methods 2009; 351, 30-35 PMC2783264 79. Rastogi, P., Sharma, J., McHowat, J. Methods for measuring the activity and expression of phospholipases A2. In: Methods in Lipid-Mediated Signaling. Murphy E & Rosenberger T (eds) Taylor & Francis. 2010; pages 4-26 80. Sharma, J., Turk, J., McHowat, J. Endothelial cell prostaglandin I2 and platelet-activating factor production are markedly attenuated in the calcium-independent phospholipase A2β knockout mouse. Biochemistry, 2010; 49, 5473-5481 PMC2938187 81. Rastogi, P., Rickard, A., Klumpp, D.J., McHowat, J. Urothelial cell platelet-activating factor production is mediated via calcium-independent phospholipase A2γ. Urology. 2011; 77, 248.e1-7. 82. Sharma, J., Turk, J., Mancuso D.J., Sims, H.F., Gross, R.W., McHowat, J. Activation of group VI phospholipase A2 isoforms in cardiac endothelial cells of wild type and knockout mice. Am J Physiol Cell Physiol. 2011; 300, C872-C879. PMID: 21191104 83. McHowat, J., Gullickson G., Hoover, R.G., Sharma J., Turk, J., Kornbluth J. Platelet-activating factor and metastasis: Calcium-independent phospholipase A2β deficiency protects against breast cancer metastasis to the lung. Am J Physiol Cell Physiol. 2011; 300,C825-C832 PMID:21228317 84. Sharma, J., McHowat, J. PGE2 release from tryptase-stimulated rabbit ventricular myocytes is mediated by calcium-independent phospholipase A2γ, Lipids, 2011; 46, 391-397. PMID: 21461868 85. Blum, J., Kinsey, G.R., Monian, P., Sun, B., Cummings, B.S., McHowat, J., Schnellmann, R.G. Profiling of Fatty Acids Released During Calcium-induced Mitochondrial Permeability Transition in Isolated Rabbit Kidney Cortex Mitochondria. Toxicol In Vitro. 2011; 25. 1001-6. PMID: 21443943 86. Sharma, J., Young, D.M., Marentette, J.O., Rastogi, P., Turk, J., McHowat, J. Lung endothelial cell platelet-activating factor production and inflammatory cell adherence are increased in response to cigarette smoke component exposure. Biochemistry, submitted 5/20/2011 87. Marentette, J., Klumpp, D.J., Rickard, A., McHowat, J. Tryptase activation of urothelial cell mitogen-activated protein kinase. Urology, submitted & under review 88. Marentette J., Rickard, A., McHowat, J. Release of prostaglandin E2 from human urothelial cells during differentiation. J. Urol., in preparation Abstracts: 1. Draper, A.J., Redfern, P.H., McHowat, J. Hypotension induced by central injection of isoprenaline and clenbuterol: Modification by propranolol. Br. J. Pharmacol. 1986; 88, 454P 2. Draper, A.J., Redfern, P.H., McHowat, J. Cardiovascular changes following administration of adrenaline and isoprenaline to the hypothalamus of the anaesthetised rat. Br. J. Pharmacol. 1987; 90, 385P 3. McHowat, J., Corr, P.B. Thrombin induced increases in lysophosphatidylcholine derived from

endothelial cells. Circulation 1991; 84, II-274 4. Wu, J., McHowat, J., Saffitz, J.E., Corr, P.B. Long-chain acylcarnitines induce reversible uncoupling in adult canine myocytes. Circulation 1991; 84, II-325 5. McHowat, J., Yamada, K.A., Saffitz, J.E., Corr, P.B. Rapid and selective accumulation of long-chain acylcarnitines in the sarcolemma of adult myocytes. Circulation 1991; 84, II-495 6. McHowat, J., Corr, P.B. Mechanisms underlying the thrombin induced increase in lysophosphatidylcholine in endothelial cells. Circulation 1992; 86, I-740 7. McHowat, J., Corr, P.B. Thrombin induced increases in lysophosphatidylcholine in adult ventricular myocytes. Circulation 1992; 86, I-821 8. Yan, G-X., Yamada, K.A., Kleber, A.G., McHowat, J., Corr, P.B. Dissociation between cellular K+ loss, reduction in repolarization time and tissue ATP levels during myocardial hypoxia and ischemia. Circulation 1992; 86, I-215 9. Yamada, K.A., Yan, G-X., McHowat, J., Kleber, A.G., Corr, P.B. Sulfonylureas partially inhibit cellular K+ loss during myocardial hypoxia without influencing action potential shortening. Circulation 1992; 86, I-4 10. McHowat, J., Yan, G-X., Yamada, K.A., Donahue, K., Kleber, A.G., Corr, P.B. Cellular uncoupling induced by long-chain acylcarnitine during ischemia. Circulation 1992; 86, I-752 11. Park, T.H., McHowat, J., Corr, P.B. Mechanisms underlying the increase in lysophosphatidylcholine induced by thrombin in adult ventricular myocytes. Circulation 1993; 88, I-326 12. Schnellmann, R.G., Blum, S.M., Miller, G.W., Creer, M.H., McHowat, J. Novel roles of phospholipase A2 in cellular injury. J. Am. Soc. Nephrol. 1994; 5, 931 13. McHowat, J., Miller, G.W., Creer, M.H., Schnellmann, R.G. The role of calcium-independent plasmalogen-selective phospholipase A2 (CIPS-PLA2) in oxidant injury. Toxicologist 1995; 15, 277 14. McHowat, J., Creer, M.H. Thrombin stimulates a calcium-independent, plasmalogen-selective phospholipase A2 (PLA2) in isolated myocytes. FASEB J. 1995; 9, A621 15. Creer, M.H., McHowat, J. Stimulation of a calcium-independent, plasmalogen-selective phospholipase A2 (PLA2) in hypoxic myocytes. FASEB J. 1995; 9 A49 16. Liu, S., McHowat, J. Interleukin-1β increases phospholipase A2 activity in adult rat ventricular myocytes, J. Mol. Cell. Cardiol. 1996; 28, A197 17. McHowat, J., Creer, M.H. Thrombin activates a unique calcium-independent, membrane-associated PLA2 in endothelial cells, J. Mol. Cell. Cardiol. 1996; 28, A148 18. McHowat, J., Creer, M.H. Activation of PLA2 is required for lysophosphatidylcholine (LPC) production following thrombin stimulation of hypoxic cardiac myocytes, J. Mol. Cell. Cardiol. 1996; 28, A198 19. McHowat, J., Creer, M.H. Isolated cardiac myocytes possess a novel, membrane-associated PLA2 activated by thrombin, J. Mol. Cell. Cardiol. 1996; 28, A197 20. McHowat, J., Creer, M.H. Thrombin activates a unique membrane-associated calcium-independent ether-phospholipid selective PLA2 in rabbit ventricular myocytes, Circulation 1996; 94, I-726 21. McHowat, J., Creer, M.H. Lysophosphatidylcholine (LPC) accumulation during ischemia is dependent on phospholipase A2 activation and augmented by net inhibition of LPC catabolism, Circulation 1996; 94, I-726 22. McHowat, J., Creer, M.H. Thrombin stimulation of endothelial cells activates a unique calcium-independent, integral membrane-associated PLA2, Circulation 1996; 94, I-587 23. McHowat, J., Tappia, P.S., Liu, S-Y., Panagia, V. Redistribution of cardiac phospholipase A2 isoforms in post-infarct congestive heart failure. J. Mol. Cell. Cardiol. 1999; 31, A29 24. McHowat, J., Creer, M.H. Selective plasmalogen catabolism by PLA2 in thrombin-stimulated myocytes. J. Mol. Cell. Cardiol. 1999; 31, A27 25. Liu, S.J., McHowat, J., Creer, M.H. Effects of lysoplasmenylcholine on membrane currents in ventricular myocytes. J. Mol. Cell. Cardiol. 1999; 31, A27

26. Kennedy, R.H., McHowat, J., Liu, S.J. The role of PLA2 and PKC isoforms in the cardiac effects of TNF-α and IL-1β. Shock, 1999 27. McHowat, J., Creer, M.H. Phospholipase A2-catalyzed hydrolysis of plasmalogen phospholipids in thrombin-stimulated platelets. Circulation 1999; 100, I-403 28. McHowat, J., O’Neill, H.B., Rohrback, J.M., Creer, M.H. Phospholipase A2-mediated phospholipid metabolism in thrombin-stimulated endothelial cells. Circulation 1999; 100, I-415 29. McHowat, J., Creer, M.H. Phospholipid metabolism in hypoxic rabbit ventricular myocytes. Circulation 1999; 100, I-274 30. Cummings, B.S., McHowat, J., Schnellmann, R.G. Inhibition of a microsomal Ca2+-independent phospholipase A2 decreases cisplatin-induced apoptosis in renal proximal tubular cells. J. Am. Soc. Nephrol. 2000; 11, 599A 31. Cummings, B.S., McHowat, J., Schnellmann, R.G. Inhibition of a microsomal Ca2+-independent phospholipase A2 increases oxidant-induced apoptosis in renal proximal tubular cells. Toxicol. Sci. 2000; 54 (Suppl), 404 32. J.McHowat, L. Swift, A. Arutunyan, N.Sarvazyan. Inhibition of cardiomyocyte phospholipase A2 activity by clinical concentrations of doxorubicin. Free Radical Biology and Medicine, 2000; 29 (S1), 135. 33. Cummings, B.S., McHowat, J., Schnellmann, R.G. Oxidant-induced oncosis and lipid peroxidation are increased by inhibition of a microsomal Ca2+-independent phospholipase A2 in renal cells. Toxicol. Sci. 2001; 60 (Suppl), 306 34. Cummings, B.S., McHowat, J., Schnellmann, R.G. Role of novel endoplasmic reticulum Ca2+-independent phospholipase A2 and 5-lipoxygenase in cisplatin-induced renal cell apoptosis. J. Am. Soc. Nephrol. 2001; 12, 799A 35. Sarvazyan, N., Swift, L.M., Kell, P.J., McHowat, J. Oxidant-induced inhibition of phospholipase A2 activity and its role in potentiation of oxidative stress., J. Mol. Cell. Cardiol. 2001; 33, A105 36. McHowat, J., and Rickard, A. Tryptase stimulation of human urothelial cells stimulates Ca-in-dependent Phospholipase A2. FASEB J. 2002; 16, A805 37. McHowat, J., and Steer, S.A. Protein kinase C-ε mediates increased Ca-independent Phospholipase A2 activity in thrombin-stimulated rabbit ventricular myocyte microsomes. FASEB J. 2002; 16, A161 38. McHowat, J., Creer, Meyer, M. Characteristics of polymorphonuclear leukocytes isolated from umbilical cord blood. FASEB J. 2003; 17, A119 39. McHowat, J., Beckett, C. Identification of thrombin-stimulated PLA2 isoforms in cardiac cells. FASEB J. 2003; 17, A119 40. McHowat, J., Wirsig, K. Activation of MAP kinases in thrombin-stimulated ventricular myocytes. FASEB J. 2003; 17, A119 41. McHowat, J., Creer, M.H., Gorges, L., Kell, P.J., Wirsig, K. Inhibition of PAF acetylhydrolase by methyl arachidonyl fluorophosphonate. FASEB J. 2003; 17, A118 42. Liu, S.J., Liu, M-Y.G., Kennedy, R.H., Creer, M.H., McHowat, J. Increases in Ca2+ cycling by lysoplasmenylcholine in adult rabbit ventricular myocytes. FASEB J., 2003; 17, A1276 43. Swift, L., McHowat, J., Arutunyan, A., Sarvazyan, N. Anthracycline analogues and inhibition of myocardial iPLA2. FASEB J. 2003; 17, A652 44. Cummings, B.S., Gelasco, A.K., McHowat, J., Kinsey, G.R., Schnellmann, R.G. Evidence for the direct inactivation of endoplasmic reticulum bound Ca2+-independent phospholipase A2 in renal cells during oxidative stress. J. Am. Soc. Nephrol. 2003 45. Meyer, M., Kell, P.J., Rickard, A., McHowat, J. Activation of calcium-independent phospholipase A2 in tryptase-stimulated human bladder endothelial cells. Cardiovasc. Pathol. 2004; 13, S114 46. Meyer, M., Kell, P.J., McHowat, J. Protein kinase C regulated calcium-independent phospholipase A2 activity in human coronary artery endothelial cells. Cardiovasc. Pathol. 2004; 13, S51

47. Crown, K.N., Kell, P.J., McHowat, J. Calcium-independent Phospholipase A2-catalyzed hydrolysis of membrane phospholipids is accelerated in human coronary artery endothelial cells exposed to hypoxia. ATVB 2004 48. Kell, P.J., McHowat, J. Release of choline lysophospholipids from endothelial cells. ATVB 2004 49. Brian S. Cummings, Andrew K. Gelasco, Gilbert R. Kinsey, Jane McHowat and Rick G. Schnellmann. Evidence for the direct inactivation of endoplasmic reticulum bound Ca2+-independent phospholipase A2 in renal cells during oxidative stress. Toxicol. Sci. 78(S-1): 93, 2004. 50. Gilbert R. Kinsey, Brian S. Cummings, Jane McHowat, Rick G. Schnellmann. Localization and Intracellular Targeting of iPLA2γ to the Endoplasmic Reticulum and Mitochondria. J. Am. Soc. Nephrol. F-P0275, 2005. 51. Gilbert R. Kinsey, Jane McHowat, Rick G. Schnellmann. Identification and characterization of iPLA2γ in mitochondria. Toxicol. Sci . 84(S-1): 2125, 2005. 52. McHowat, J., Meyer, M.C. Role of platelet-activating factor in the recruitment of mast cell precursors to atherosclerotic plaques. Experimental Biology 2005 53. Rickard, A., McHowat, J. Characterization and reepithelialization of urothelium. Experimental Biology 2005 54. Vinson, S.M., McHowat, J., Rickard, A. Inhibition of PAF acetylhydrolase by MAFP in bladder microvascular endothelial cells Experimental Biology 2005 55. Kinsey, G.R., Cummings, B.S., McHowat, J., Schnellmann, R.G. Localization and intracellular targeting of iPLA2γ to the endoplasmic reticulum and mitochondria. J. Am. Soc. Nephrol. 2005; F-P0275 56. Kinsey, G.R., McHowat, J., Schnellmann, R.G. Divergant roles for iPLA2γ in mitochondrial dysfunction. FASEB J. 599; 3, 2006 57. Gilbert R. Kinsey, Jane McHowat, Rick G. Schnellmann. iPLA2γ is Required for Mitochondrial Function and Viability in Renal Proximal Tubular Cells. J. Am. Soc. Nephrol. F-P0906, 2006. 58. Meyer, M.C., McHowat, J. Potential mechanism for mast cell progenitor transendothelial migration Atherosclerosis 2006; 7, S223 59. White, M.C., McHowat, J. A potential mechanism for mast cell progenitor transendothelial migration FASEB J. 2007; 21, A 903 60. Rastogi, P., McHowat, J. Mast cell tryptase may play a protective role in early inflammation in human small airway epithelial cells FASEB J. 2007; 21, A958 61. McHowat, J., Rickard, A., Dorokhov, N., Klumpp, D. Loss of prostaglandin E2 release from urothelial cells isolated from an interstitial cystitis patient. FASEB J. 2007; 21, A765 62. McHowat, J., Rickard, A. Expression of ZO-1, ZO-2, and ZO-3 proteins in a urothelial cell culture system. FASEB J. 2007; 21, A763 63. Rastogi, P., McHowat, J. Inhibition of calcium-independent phospholipase A2 in pulmonary microvascular endothelium prevents inflammatory mediator production FASEB J. 2007; 21, A862 64. Gilbert R. Kinsey, Jane McHowat, Rick G. Schnellmann. Knock-down of iPLA2γ Results in Lipid Peroxidation, Mitochondrial Dysfunction and Death in Renal Cells. Bioactive Lipids in the Lipidomics Era Keystone Conference 2007, Poster #124. 65. Gilbert R. Kinsey, Jane McHowat, K. S. Patrick, Rick G. Schnellmann. Role of iPLA2γ in renal cell mitochondrial permeability transition. Toxicol. Sci. 96(S-1): 1274, 2007. 66. Rastogi, P., Young, D.M., White, M.C., McHowat, J. Transendothelial migration of CD133+ hematopoietic progenitor cells isolated from human umbilical cord blood. Experimental Biology 2008 67. Rastogi, P., Young, D.M., McHowat, J. Thrombin induces calcium independent phospholipase A2 (iPLA2) activity and neutrophil adherence in human small airways epithelial cells. Experimental Biology 2008 68. Rastogi, P., McHowat. Thrombin activates calcium independent phospholipase A2 (iPLA2) activity in lung microvascular endothelial cells. Experimental Biology 2008

69. Rickard, A., Marentette, J., Mchowat, J. Characterization of stratification and tight junction formation in cultured human urothelial cells. Experimental Biology 2008 70. Rastogi, P., Dorokhov, N., Rickard, A., McHowat, J. Impaired prostaglandin E2 (PGE2) production in urothelial cells from an interstitial cystitis patient. Experimental Biology 2008 71. McHowat, J., Young, D.M. Recruitment of inflammatory cells to the lung is dependent upon platelet activating factor production in smokers. Experimental Biology 2010 72. Sharma J., McHowat, J. PGE2 release from tryptase-stimulated rabbit ventricular myocytes is mediated by calcium-independent phospholipase A2γ. Experimental Biology 2010 73. McHowat, J., Rickard A., Marentette, J. Impaired expression of prostaglandin E2 synthesis and degradation enzymes in urothelial cells from patients with interstitial cystitis. Experimental Biology 2010 74. Sharma, J., McHowat, J. Activation of calcium-independent phospholipase A2β increases platelet activating factor and PGI2 production in cardiac endothelial cells. Experimental Biology 2010 75. McHowat, J., Marentette, J., Klumpp, D.J., Rickard, A. Tryptase activation of urothelial cell mitogen-activated protein kinase, FASEB J March 17, 2011 25:791.6 76. McHowat, J., Gullickson, G., Hoover, R.G., Sharma, J., Turk, J., Kornbluth, J. Calcium-independent phospholipase A2β protects against breast cancer metastasis to the lung, FASEB J March 17, 2011 25:820.1 77. Sharma, J., Hoft, D.F., McHowat, J. Increased platelet activating factor production in T. cruzi infected endothelial cells, FASEB J March 17, 2011 25:638.10 78. Sharma J., McHowat, J. Activation of calcium-independent phospholipase A2 following protease-activated receptor cleavage in mouse cardiomyocytes, FASEB J March 17, 2011 25:1112.6 79. Sharma, J., Eickhoff, C.S., Hoft, D.F., McHowat, J. Absence of iPLA2β does not affect recruitment but impairs function of inflammatory cells in T. cruzi-induced myocarditis. Submitted to American Heart Association Scientific Sessions, November 2011 80. McHowat, J., Sharma, J., Kornbluth, J. Role of iPLA2β in breast cancer metastasis, 12th International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases September 18-21, 2011, Seattle, WA