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DOI: 10.1177/00220345640430032501

1964 43: 462J DENT RESA Simplified Technique for the Preparation of Ground Sections

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A S i m p l i f i e d T e c h n i q u e f o r t h e P r e p a r a t i o no f G r o u n d S e c t i o n sD . F . WEBERD e p a r t e n t o f H i s t o l o g y , . l f a r q l e / l e U n i 7 ! c r s i t s , S c / h o o l o f D e n t i s t r y , 1 1 i l w o r k e r , 1 1 ' i c o n s ( i nI n c r e a s e d I u s e o f m i i c r o r a d l i g r a p h y d l u r i l l g t h e p ~ a s t ( l e c a d l e h a s g i v e n u I S much v x a l u a l - i l e i n f o r m a t i o n c o n -c e l r n i n g t h e n i u n e r a l i z e t i d l e n t a l t i s s u e s . C o n s i d e r a b l e r e f i n e n i e n t i n t h e m i e t h o ( d h a s t a k e n p l a c e , b u t t h ep r o b l e m o f p r e p a r i n g s u i t a l I l e g r o u n t i s e c t i o n s i s s t i l l a p e r t i n e n t o n e . M e c h a n i c a l s e c t i o n i n g m a c h i n e sa r e e x c e l l e n t ) l t t h e i r i n i t i a l c o s t i s h i g h . , a n d i t i s i m l p o s s i l l e c o n s i s t e n t l y t o c u t s e c t i o n s b e l o w 3 0 P i nt h i c k n e s s . S e c t i o n p i r e p a r a t t i o n b y h a n d g r i n d i n g t e c h n i q u e s i l e s c r i l e t l i n l t h e l i t e r a t u r e i s a t l e s t v e r yt i m e C o n s u m i n g . T 1 h l m i c t h o d t ol i e s l e s c r i b e e d h e r e e m b o d i e s s o n i c o f t h e t e c hn ic a l p r o c e d u r e s u s e d i nt h e h a n d g r i n d i n g m e t h o d s l i u t h a s a m o d i f i c a t i o n t h a t a p p i r e c i a l i l y r e d u c e s t h e ) r e p a r a t i o n t i m e .T I ' h e t i s s u e t o b e s e c t i o n e d i s i m b e d d e d i l l a p l a s t i c m e d i u m . M e t h y l m e t h a c r y l a t e i s u s e d r o u t i n e l y .' F h e b l o c k c o n t a i n i n g t h e t i s s u e i s t h e n c u t t o t h e d l e s i r e d p l a n u s i n g i t c o n v e t i t i o t l d d e n t a l m o d e lt r i m m n i e r . T h e g r i n d l i n g w h e e l o f t h i s m a c h i n e l e a v e s a r e m i a r k a l b l y s m o o t h s u r f a c e . I f C i c s i r e c h o w e v e r ,t h i s s u r f a c e mav l i e p o l i s h e d f i x a n y o f t h e m e t h o d s p r e v i o u s l y d e s c r i b e d i n t h e l i t e r a t u r e . F r e m l i n ,M t a t h i e s o n , a n dl - F a r d I \ i c k ( A r / i . r a i B i o t l . , 5 : 5 5 - 6 0 , 1 9 6 1 ) h a v e r e p o r t e d l y a i n e x c e l l e n t m e t h o d . N o . 4 / 0e m e r y p o l i s h i n g p a p e r f o l l o w e d b v y j e w e l e r s ' r o u g e N x a s f o u n d t o i e q u i t e s a t i s f a c t o r y T h i s s u r f a c e i st h e n g l u e d t o a p l i e c e o f g l a s s I i n i c h s q u a r e w i t h a c o m m e r c i a l a d h e s i v e . * T F h e s e g l a s s s q u a r e s a r e c u t f r o mt l l i c r o s c o l e s l i d e s . \ c o r k i s g l u e d l t o t h e o p i ) o s i t e s i d e o f t h e s l i d e w i t h t h e c e n t r a l p l o r t i o n o f t h e s e c t i o no v e r t h e c e n t e r o f t h e c o r k . ' I h i s a c t s i t s a h a n d l l e f o r m r a n u a l l y a t l v a n c i n g t h e s p e c i m e n i n t o t h e b l a c l eo f t h e m o d e l t r i m i m n e r . U s i n g t h e a r e a o f t h e w h e e l n e a r e s t t h e h u b r e ( l u c e s t h e a m o u n t o f v i b r a t i o n a n dl ) r e v e n i t s f r a c t u r e o f t h e s p e c i m e n . S e c t i o n s o f 2 0 - - 3 0 g can b ) e r o u t i n e l y p r e p ) a t r e ( l l s x t h i s t e c h n i q u e . I ft h i n n e r s e c t i o n s a r e r e q u i r e d , t h e s p e c i m e n c a n l i e f u r t h e r r e d u c e d b y h a n d g r i d l i n g t h e s p e c i m e n ( w h i l es t i l l on t h e g l a s s s l i d e ) x i t h c a r b o r u l u n d u m a b r a s i v e e p a p e r f o l l o w e d l y e n i e r x p a p e r . S e c t i o n s i r e r e m o v e df r o m t t h e s l i d e b y i m m e r s i o n i n w a r m i . w a t e r f o r s e v e r a l h o u r sT h i s m e t h o d r e d u c e s t h e t i m e r e q u i r e d t o p r e p a r e s e c t i o n s i n a i ( l e r l u x g e o f t h i c k n e s s e s a n d ( u t i l i z e si n e x p e n s i v e c l u i p i m e n t t h a t i s r e a d i l y ' a v a i l a b l e ( c t m e n t a l l i n s t i t u t i o n s . T h e a c c o n i p l a n > i n g p h o t o g r a p h so f i t g r o u n d s e c t i o n o f d e n t i n ( 2 t j u ) a n d ( r a l d i o g r a p h o f a s i m i l a r a r e a i n ( l i c a t e t h e e l l I c a c y o f t h e t e c h

n l t t i c .

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M ' r . W i ~ l l i i o n M i n n i s r e s p o n s i b l e f o r t h e e x c e l l e n t p h l o t o m n i c r o g r a p h s .A d d f i t - i o n a l l i n f o r m a t i o n i s a v a i l a b l e o n r e q u e s t t o t h e a u t t h o r s .R 4 e c e i v e d l f o r p u b l i c a t i o n l O c t o b l e r 3 0 , 1 9 6 3 .* E a s t m a n 1 9 1 0 a d h e s i v e , T e n n e c s s e e E 1 a s t m l a n ( C o . , K i n g s p o r t , ' L e o n i .

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