Isolation and Mass Cultivation of Pigment Producing Bacteria
Transcript of Isolation and Mass Cultivation of Pigment Producing Bacteria
ISOLATION AND MASS CULTIVATION OF PIGMENT PRODUCING BACTERIA
INTENT:y To Isolate the Organism from the Source y To Purify and Obtain Axenic Culture y Maintenance of Axenic Culture y Characterization of the Organism(Physiological&
Biochemical) y Mass Multiplication of the Organism y Pigment Extraction(Solvent Extraction)
ISOLATION:y Source(Rhizosphere-Soil-1gm) y Serial Dilution y 10 -5 and 10-6
Kings B base Medium y Streak and Pour Plate y RESULT: Orange Coloured Colonies(Round, Raised, Mucoid )
PURIFICATION:y Single Colony Transferred in to Nutrient Agar y Quadrant Streaking y Single Colony to NA Slant
MAINTENANCE:y Colonies were Transferred to New NA Slants Every Two
Weeks and Maintained
CHARACTERIZATION:y PHYSIOLOGICAL(Gram ve, Rod, Non Motile) y BIOCHEMICAL(Catalase ve, Oxidase ve,
TSI test-Pink Colonies, Citrate ve, Indole Test +ve)
MASS CULTIVATION:y Pure Colonies From NA Slant y Inoculated in 250 ml of Nutrient Broth y Incubated in Rotary Shaker for Five Days at 37 degree