Iron-Reducing Bacteria
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Transcript of Iron-Reducing Bacteria
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Iron-Reducing Bacteria
Hopkins Microbiology Course
Presented by Ying Lei22 July 2010
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Reduction Process
1. Fe3+ only– ferrihydrite (FeOOH · 0.4H2O)– orange/red solid
2. Fe3+/Fe2+
– magnetite (Fe3O4)– black, ferromagnetic solid
3. Fe2+ only– ferrous iron– black, non-magnetic solid
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Redox TowerReduced Species Oxidized Species Redox Potential (E0’)
[CH2O] CO2 -470 mV
Fe2+ αFe2O3 -280 mV
Fe2+ αFeOOH -280 mV
CH4 CO2 -250 mV
H2S SO42- -220 mV
Fe2+ γFeOOH -100 mV
Fe2+ Fe(OH)3 0 mV
Fe2+ Fe3+ +770 mV
H2O O2 +820 mV
CH3CH(OH)COO- + 12 FeOOH + 4 H2O 3 HCO3- + 12 Fe2+ + 22 OH-
lactateC0
iron (III)Fe3+
CO2
C4+
iron (II)Fe2+
Canfield et al, Aquatic Geomicrobiology (2005)
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Enrichment MediumComponent Amount Purpose
basal marine mineral medium 1 L basic salts
sodium lactate 4.5 g (30 mM) carbon source
NaHCO3 30 mL (30 mM) pH buffer
iron (III) hydroxide 30 mL (40 mM) ferric iron source
7-vitamin solution 30 mL cofactors
SL 10 1 mL trace elements
Se-Wo solution 1 mL trace elements
Adjust to pH 7.2 in Pfennig bottles and incubate under anoxic conditions.
Hopkins Microbiology Course (2010)
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Sample Collection
Canfield et al, Aquatic Geomicrobiology (2005)
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Sample Collection
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Successful Enrichments
Marcus
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Thank You
All Instructors and Students
ChrisMarcusBlanca
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Additional Slides
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Iron Solubility
OzCoasts (www.ozcoasts.org.au)