IPM IMPACT

ELO BRUSSELS APRIL 2013

IPM IMPACT LINTER

BELGIUM

IPM IMPACT

ACTIVITIES

Side-effect trials on bumblebees (several species – subspecies)

Side-effect trials on beneficial organisms

• Microbials

• Predators

• Parasitoids

• Nematodes

Efficacy trials (mainly microbials, botanicals, ….)

Problem solving :

• New pests e.g. Aculops, Tuta

• Rearing insects/mites

Resistance monitoring

Fytotox trials

EU dossiers microbials (renewal PreFeRal annex I)

Quality trials microbials

April 25, 2013

TELLING

Predators/Predatory mites

Parasitoids

HIK GEEL

SEMI-FIELD TRIALS

TESTING ORGANISMS (1)

• Predators

• Macrolophus caliginosus : Larvae

• Nesidiocoris tenuis : Larvae

• Orius insidiosus : Larvae

• Orius laevigatus : Larvae

• Anthocoris nemoralis : Larvae

• Delphastus catalinae : Larvae

• Adalia bipunctata : Larvae

• Coccinella septempunctata : Larvae

• Harmonia axyridis (nofly) : Adults

• Atheta coriaria : Larvae

• Aleochara bilineata : Larvae

• Forficula auricularia : Larvae

• Chrysoperla carnea : Larvae

• Aphidoletes aphidomyza : Larvae

• Feltiella acarisuga : Larvae

• Episyrphus balteatus : Larvae

TESTING ORGANISMS

(2)

Parasitoids: most sensitive stage: adults • Encarsia formosa : adults

• Encarsia formosa : larvae in whiteflies

• Eretmocerus eremicus : adults

• Eretmocerus eremicus : larvae in/under whiteflies

• Eretmocerus mundus : adults

• Eretmocerus mundus : larvae in/under whiteflies

• Aphidius colemani : adults

• Aphdius colemani : larvae in aphids

• Aphidius ervi : adults

• Aphdius ervii : larvae in aphids

• Lesiphlebus testaceipes : adults

• Lesiphlebus testaceipes : larvae in aphids

• Dacnusa sibirica : adult

• Dacnusa sibirica : larvae in leafminers

• Diglyphus iseae : adult

• Diglyphus iseae : larvae close to leafminers

• Trichogramma spp. : adult

• Trichogramma spp. : larvae in egg

TESTING ORGANISMS

(3)

Predatory mites: most sensitive stage: adults

• Amblyseius swirskii : mixed population (breeding system or

direct release)

• Amblyseius cucumeris : mixed population (breeding system or

direct release)

• Amblyseius degenerans : adults

• Amblyseius montdorensis: adults

• Amblyseius californicus : mixed population (breeding systyem)

• Amblydromalis limonicus : mixed population

• Phytoseiulus persimilis : adults

• Hypoaspis miles : mixed population

• Hypoaspis aculeifer : mixed population

Remark: Hypoaspis spp. can also be used as a test species for herbicide

trials

TRIGGERED TRIALS

Fungicides 2 bloc treatments, 2 assessments (FRAC)

Residual activity (0, 1, 3 , 7 and 14 days)

Sublethal effects (fecundity, behaviour,…)

Application method (irrigation, spraying, dusting, …..)

25 April 2013

SIDE EFFECTS TRIALS (1)

(WORST CASE SCENARIO)

Side Effects Trials

Direct toxicity

Release

testorganism

on dry residu

(IOBC/EPPO – Protocols)

Residual toxicity

Release test-organism

on residu

Application

directly

on existing population

( Practical conditions)

Application Application

assessment

Plants are set aside for:

0, 1, 3 days, 1 week, 2 weeks

25 April 2013

SIDE EFFECTS TRIALS (2)

(WORST CASE SCENARIO)

If triggered

Predator/prey ratio

Parasitoid/host ratio

Abbott value

Recovery

(class 5 – eradication)

Mortality (predators)

Parasitation and Hatching rate (parasitoids)

Mortality (predators)

Parasitation and

Hatching rate

(parasitoids)

Application Efficacy pest

Conclusion

Surviving beneficials are

collected and released on

non-sprayed plants

25 April 2013

SIDE EFFECTS TRIALS (3)

(PRACTICAL CONDITIONS)

If triggered (2)

Different concentrations

Different spraying volumes

Abbott value

Selectivity in time

Selectivity in place

Evaluation

Mortality (predators)

Parasitation and

Hatching rate

(parasitoids)

Biology beneficial Efficacy pest

End Conclusion

e.g. spot treatments

e.g. after diapauze

e.g. start or end season

e.g. before imigration

BIOBEST N.V. SIDE EFFECT LIST

SIDE-EFFECT LIST

SIDE-EFFECT LIST

SIDE-EFFECT LIST

THE WORLD MOST COMPREHENSIVE DATABASE ON

SIDE-EFFECTS ON BIOLOGICAL AGENTS AND POLLINATORS

• MORE THAN 13000 DATA FROM OUR OWN RESEARCH OR FROM

COOPERATION WITH RELIABLE SOURCES.

• RESULTS ON 100+ BENEFICIAL ORGANISMS.

• MORE THAN 500 ACTIVE INGREDIENTS.

• SEVERAL THOUSANDS OF TRADE NAMES.

• EFFECTS OF INSECTICIDES, ACARICIDES, FUNGICIDES,

HERBICIDES, PLANT GROWTH REGULATORS, MICROBIALS,

NUTRIENTS AND PHYSICAL COMPOUNDS ON ALL IMPORTANT

AND RELEVANT BENEFICIAL ORGANISMS, POLLINATORS

INCLUDED.

• UPDATED EVERY MONTH WITH NUMEROUS NEW RESULTS

COOPERATION

ENTOFILM

COOPERATION

ENTOFILM

CASE STUDY: IMIDACLOPRID AND

POLLINATORS

- Working group pollinators and pesticides NL

- 80’s-early 2000: several cases of toxicity of organophosphates, carbamates, etc. every

year to honey bees

- Since 2005: hardly a case of mortality of honeybees due to the use of pesticides despite

huge use of neonicotinoids

- Stop the group?

- 1 case in 2012: 1 illegal use of a non-neonicotinoid

- Australia : no abnormal mortality but no varroa mite

- Science article on bumblebees (Felix Wackers)

- Good experimental science

- Realistic dose rate: improvement

- But: far too long exposure with this dose rate: completely unrealistic!!!!!

- Typical ivory tower work from university scientists

- Conclusions to bumblebees in environment: not correct

- OPERA project: bee health in Europe - Fact and figures 2013

- No strong evidence that lethal or sublethal effects play a role in bee colony mortality

FLYING DOCTORS IN

MULTIHIVE