Ion Exchange worksheet

Due at the beginning of lecture on Thursday, April 7th, 2011

Ion exchange worksheetdue April 7th

• Please hand in a neatly presented (typed):

1. Table with absorbance and corresponding concentration values

2. A graph of your GFP standard curve, be sure to label the axis

3. The concentration of your purified GFP (mg/ml)

4. The total amount of GFP purified (mg)

5. The volume (ml) of your purified GFP

6. Percent recovery: assuming your initial sample mixture (0.5ml) contained 1.0mg/ml GFP

Note: If you diluted your purified GFP remember to use the dilution factor in your calculations