Intracellular potentials - Cornell University

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Intracellular potentials

Transcript of Intracellular potentials - Cornell University

Page 1: Intracellular potentials - Cornell University

Intracellular potentials!

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Extracellular! Intracellular!

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Intracellular microelectrotrode!

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Replacing air with KCl in electrodes!

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Intracellular microelectrotrode!

0.1 micron!

Electrode resistance (10 to 30 Mohms)!

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RC response of electrode to current injection!

Time constant?!

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Electrode capacitance!

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AgCl! Ag+ + Cl-!

Ag!

+e-! -e-!Conduction in metal!

Conduction in saline solution!

DC charge transfer on electrode wire!

Extracellular!

capacitative charge transfer!

Intracellular!

direct charge transfer!

Very low solubility!

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Current injection into cells!

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What do these have in common?!

! “rubbing salt in a wound”!

! Lethal injection cocktail!

! Dr. Kevorkian’s suicide machine!

! Hyperkalemia!

! Hypokalemia!

Resting Potential!

O mV!

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Membrane ion distributions!

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Ionic basis of the resting potential!

Unequal distribution of ion species across membrane!

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Selective permeability of ion species through membrane!

Loss of K??- Q = CV 10-5 M K for -58 mV!

Donnan Equilibrium!

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Squid giant axon- a model prep!

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40,000 APs can fire!!

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At Rest: INa = IK?!

! IK = gK (Vm-EK) INa = gNa (Vm-ENa)!

gK (Vm-EK) = gNa (Vm-ENa) !

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Other contributions to RP?!

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Changes in “ RP” by changing ionic conductances!


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