Interview Questions - Set 2
Transcript of Interview Questions - Set 2
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Which enzyme is incharge ofsynthesizing themajority of theribosomal RNA?
DNA Polymerase RNA Polymerase I RNA Polymerase II Ribosome Polymerase 3
Which of thefollowing is NOT a
type of spontaneousmutation?
Tautomerism Depurination Slipped strand mispairing Base analog guidedmutation
4
Mis-sense mutationsare the ones which:
Code for a differentamino acid than theoriginal one.
Code for the sameamino acid as theoriginal one.
Code for same amino acidas the original one butwith a different function.
Code for a stop and cantruncate the protein.
1
Which of thefollowing is not a"wobble base pair"?
Guanine-Uracil Inosine-Uracil Inosine-Adenine Inosine-Guanine 4
In which of thefollowing DNAregions does thetranscriptioninitiation take place?
Operator Promoter Regulator Initiator 2
Which gene normally
codes for regulatoryproteins controllingcell growth and celldifferentiation?
Growth gene Proto-oncogene Hormone gene Immunoglobin gene 2
What are DNA,consisting of verylarge arrays ofcyclically repeating,non-coding DNAfragments known as?
Euchromatin Heterochromatin Telomere Satellite DNA 4
Which of thefollowing processescan be used todeterminetranscriptioninitiation site?
Foot Printing Northern Blotting Primer Extension Nick Translation 3
Which of thefollowing is notrequired whilemaking DNA probesthrough the Nick
Translation process?
DNA Polymerase DNase Deoxyribonucleotides Primers 4
Which of thefollowing techniquesdid Messelson andStahl use to showthat DNA replicationis semi conservativein nature?
Radioactivity Centrifugation Chromatography Dialysis 2
In Griffith'sexperiment, which of
the following areINCORRECT withrespect toStreptococcuspneumoniae for theprocess oftransformation?
The extract ofbacteria is treated
with lipases,proteases and RNasedid not jhave anyeffect on the abilityto infect the mouse.
The live R strain ofStreptococcus
pneumoniae waspotentially ineffectiveto infect the mouse.
The dead S strain ofStreptococcus pneumoniae
was potentially ineffectiveto infect the mouse evenwhen mixed with live Rstrain.
The extract ofStreptococcus
pneumoniae having liveR starin and dead Sstrain could infect themouse.
2
Which of thefollowing enzymes isproduced by lacoperon?
Beta-lactamase Alpha-amylase Beta-galactosidase Alpha-galactosidase 3
Which of thefollowing virusesdoes not have DNA
Tobacco MosaicVirus
T2 bacteriophage Small pox virus Herpes virus 1
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as genetic material?
Which of thefollowingchromosomes is aspecial type ofchromosome foundin amphibians, birdsand insects but notfound in mammals?
Lampbrushchromosome
Adrenal glandchromosome
Micro chromosome Lampbeadchromosome
1
What is the correctsequence of stagesof cell division inmeiosis?
Pachytene,Diplotene,Diakinesis,Leptotene, Zygotene
Leptotene, Zygotene,Pachytene,Diplotene, Diakinesis
Diplotene, Leptotene,Zygotene, Pachytene,Diakinesis
Diplotene, Leptotene,Diakinesis, Pachytene,Zygotene
2
Which of thefollowing enzymescauses negativesuperhelical turnsduring DNAreplication?
DNA Gyrase DNA Polymerase Helicase DNA Primase 1
Which of thefollowing is true forthe parts of DNAdouble helix that
tend to separateeasily?
They have high GCcontent and high ATcontent.
They have high GCcontent and low ATcontent.
They have low GC contentand high AT content.
They have low GCcontent and low ATcontent.
3
Which of thefollowing parts ofmessenger RNA hasthe start codon?
Leader sequence Trailer sequence Reading frame Promoter region 3
Which of thefollowing proteins isinvolved in folding ofProkaryotic genome?
Protein SR Protein HU Polymerase Helicase 2
With which of thefollowing enzymesdoes primase form acomplex in order toassist in DNAreplication?
DNA Helicase DNA Polymerase DNA Ligase DNA Nuclease 1
Which of thefollowing causestranscription to stop?
When RNAPolymerase meets astop codon.
When DNAPolymerase meets astop codon.
When DNA Polymerasemeets a terminatorsequence.
When Taq Polymerasemeets a stop codon.
4
Which of thefollowing enzymes isa part of primosomecomplex that causesDNA replication?
Ligase DNA Polymerase Helicase Nuclease 3
Which of thefollowing enzymescleaves the RNAprimer from the DNAmolecule duringDNA replication?
RNase H RNase A DNA Polymerase II DNA Polymerase I 4
Which of thefollowing is true forDNA replication?
It takes place in 5'-3'direction and is semiconservative innature.
It takes place in 5'-3'direction and isconservative innature.
It takes place in 3'-5'direction and isconservative in nature.
It takes place in 3'-5'direction and is semiconservative in nature.
1
Proof reading is a/an_______.
DNA Gyrase activity 3'-5' Exonucleaseactivity
5'-3' Exonuclease activity Endonuclease activity 2
Which of thefollowing is a featureof homologousgeneticrecombination?
Holliday intermediate Klenow fragments Transposition Replisome 1
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Which of the
following proteinsactivatestranscription in E.Coli?
cAMP receptor
protein
Transcription factor Humulin ATP-binding proteins 1
Which of thefollowing bases is
not found in RNAgenetic code?
Adenine Guanine Thymine Cytocine 3
Which of thefollowing maximizesthe number ofcodons that can beread by a particulartRNA molecule?
Inosine Xanthosine Thymidine Guanosine 1
Upstream Activation
Sequence (UAS) inyeast is also knownas _______.
Adaptor Enhancer Promoter Activator 2
Which of thefollowing groups inGuanine is
responsible forGuanine not beingable to form adouble bond withAdenine?
NH2 OH CH3 SH 1
What is the functionof photolyases?
It cleavesphosphodiesterbonds at the end of apolynucleotide chain.
It bindscomplementary DNAstrands and breakscertain types ofpyrimidine dimers.
It cleaves phosphodiesterbonds within apolynucleotide chain.
It repairs single-stranded discontinuitiesin double strandedDNA molecules.
2
Which of thefollowing enzymes isused to join DNAfragments together?
Nucleases Kinases Ligases DNA Polymerases 3
Which of thefollowing groups ofenzymes areresponsible for baseexcision repair in E.Coli, a mechanism
by which damagednucleotides in DNAare removed andreplaced?
DNA Glycosylase, APEndonuclease, DNALigase and DNAPolymerase.
AP Endonuclease,Photolyase, DNALigase and DNAPolymerase.
DNA Glycosylase, Kinase,DNA Ligase and DNAPolymerase.
Kinase, Photolyase,DNA Ligase and DNAPolymerase.
1
The lac repressorprotein is a
________.
Dimer Trimer Tetramer Hexamer 3
Which of thefollowing is a featureof 'Restriction
enzyme'?
(i) It cuts doublestranded DNA atspecific sites.(ii) It cuts themethylated DNA.(iii) It makes twoincisions, one oneach strand, in thesugar-phosphateback bone.
(i) and (ii) (i) and (iii) (ii) and (iii) (i), (ii) and (iii) 2
Beginning with 30template DNAmolecules, howmany amplicons willbe produced after 25
2x1010 molecules 1x109 molecules 1x1010 molecules 2x109 molecules 2
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cycles of Polymerase
Chain Reaction?
What are homingendonucleases?
They are DNaseencoded by intronsor inteins.
They are DNaseencoded by exons.
They are DNase found inbacteria.
They are DNAPolymerases.
1
Which of thefollowing is false for
restrictionendonucleases?
They are found inEukaryotes.
They were developedas defense
mechanism againstinvading viruses.
HindII was the firstrestriction endonuclease to
be isolated.
They do not requireany other enzyme
activity to cut thestrands of DNA.
4
Which of thefollowing enzymescuts the 3' hairpinloop during theconstruction ofcDNA?
S1 Nuclease DNA Polymerase RNA Polymerase Ribonuclease 1
Transformation doesnot work well forlinear DNA because:
DNA does not getintegrated into themain chromosome.
Exonuclease insidethe bacteria rapidlydegrades the DNA.
DNA gets degraded byheating the step at42C.
DNA is cleaved by DNAPolymerases.
2
Which of thefollowing principlespermits heating andcooling of blocks by
reversing the electriccurrent in a thermalcycler (PCRmachine)?
Chemiluminescence Peltier effect Doppler effect Electromagnetic effect 2
Which of thefollowing is an ionicdetergent used inPolymerase ChainReaction whichprevents formationof secondarystructure?
Tween-20 Dimethyl Sulfoxide(DMSO)
NonylPhenoxypolyethoxylethanol(NP-40)
Triton-x 100 2
Which of thefollowing classes ofrestriction enzymescleaves methylatedDNA?
Type-I Type-II Type-III Type-IV 4
Taq Polymerase usedin Polymerase ChainReaction is a heatstable _______.
RNA Polymerase DNA Polymerase mRNA Polymerase tRNA Polymerase 2
Which of thefollowing moleculesdoes pyrosequencingoperate on?
Protein DNA RNA Lipid 2
Which of thefollowing is best forDNA storage?
Normal saline Tris EDTA Distilled water Acetic acid 2
SmaI is a restrictionenzyme that
produces ______.
Blunt ends only Sticky ends only Both blunt and sticky ends Depends upon themolecule being cleaved.
1
Which of thefollowing is true for
Type I restrictionenzyme?
It has ligase activity. It has methyltransferase activityonly.
It has both restriction andmethyl transferase activity.
It has both ligase andmethyl transferaseactivity.
3
Which of thefollowing is true forM13 bacteriophageDNA replication?
It uses the negativestrand of ReplicatingForm (RF) of thephage as template toform new strands.
It uses the positivestrand of ReplicatingForm (RF) of thephage as template toform new strands.
The phage uses its ownproteins to synthesize thenew strands.
It can use eitherpositive or negativestrand of theReplicating Form (RF)of the phage astemplate to form newstrands.
1
INTERVIEW QUESTIONS - SET 2
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How many fold
amplifications wouldhappen if a targetsequence, whoseflanking sequence isunknown, undergoes5 cycles in PCR?
25 fold 125 fold 3125 fold 0 fold 4
According to theMenedelism, a typicalmonohybrid crossshows thephenotypic ratio of3:1. Whatpercentage of theoffsprings will behaving Bb genotypesif both of the parentsare having Bbgenotypes in amonohybrid cross?
0.25 1 0.5 0.75 3
What is a sepiasomiccondition?
It is a decrease inchromosomenumber (2n-2).
It is an increase inchromosome number(2n+5).
It is an increase inchromosome number (3n).
It is a decrease inchromosome number(2n-1).
2
Which of thefollowing has lost itsprotein codingability?
Pseudogenes Introns Exons Inteins 1
Which of thefollowing types ofgenome annotationsare ORFs related to?
Functionalannotation
Physical annotation Chemical annotation Structural annotation 4
What is percentrecombination in atest cross equal to?
Double cross over Single cross over Map-distance Map area 3
Mutation in SNC Agene that causesParkinson Diseasehas been mapped at
____________.
Chromosome 7 Chromosome 4 Chromosome 5 Chromosome 1 2
Which of thefollowing diseasesillustrates Mendel's
law?
Fragile X syndrome Huntington's disease Arthritis Sickle cell anemia 4
The differences ingenome sizes withina class of organismsare almost entirelydue to differences inthe amount of
_______.
Protein coding DNA Non protein codingDNA
Protein coding RNA Non protein codingRNA
2
Which of thefollowing enzymes
finds and removes
RNA primers duringDNA replication?
RNA Primase RNA Polymerase DNA Polymerase epsilon DNA Polymerase I 4
In direct reversalDNA repairmechanism, whattype of abnormalbond betweenadjacent pyrimidinebases is the result offormation ofpyrimidine dimersupon irradiation withUV light?
Phosphodiester bond Covalent bond Peptide bond Glycoside bond 2
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Which one of the
following is the moststable?
DNA-DNA Duplexes mRNA-rRNA
Duplexes
RNA-DNA Hybrid mRNA-tRNA Hybrid 3
Which of thefollowing paths doesthe 'proteintranslation from
mRNA' take?
N-terminus toC-terminus
C-terminus toN-terminus
N-terminus to N-terminus C-terminus toC-terminus
1
_____________ is adiverse group oflong primarytranscripts formed inthe eukaryoticnucleus, many ofwhich will beprocessed to mRNAmolecules by
splicing.
Heterogeneousnuclear RNA
Homogeneousnuclear RNA
Large nuclear RNA Small nuclear RNA 1
In genetictranscription, whichshort DNA sequenceis the most commontype of core
promoter ineukaryotes?
Transcription FactorII B
Transcription FactorII D
TATA Box Template Strand 3
Which of thefollowing anticodonsdoes the tRNAshave, that arecomplementary tothe codon in mRNAand can be chargedcovalently withamino acids?
3' terminal UGC ends 3' terminal AAU ends 3' terminal UGU ends 3' terminal CCA ends 4
Which of thefollowing mutationscauses Tay-Sachsdisease?
Point Mutation Missense Mutation Nonsense Mutation Frameshift Mutation 4
Which one of thesemethods can be usedfor analyzing thepattern ofmethylation in aspecific region ofDNA?
Methylation-specificPCR (MSP)
Pyrosequencing Southern Blotting Bisulfite Mapping 4
What do missensemutations code for?
Amino acid thatcodes for stop signal
Different amino acid Same amino acid Amino acid that codefor a change
2
Which type ofrestriction enzymestarget methylatedDNA?
Type I Type II Type III Type IV 4
In Cos sequences,
which site holds the
terminase while it isnicking andseparating thestrands?
Cos B Cos N Cos Q Cos X 1
What is a terminalDeoxynucleotidyl
Transfrerase?
A nuclease A ligase A polymerase A lipase 3
Which of thefollowing is SOLiDsequencingspecifically used for?
dNTPs ddNTPs Oligonucleotides Oligonucleosides 3
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Which technique is
used for producingtemplates forpyrosequencing?
Solid Phase
TemplatePreparation
Liquid Phase and
Enzymatic TemplatePreparation
Enzymatic Template
Preparation
Liquid Phase Template
Preparation
Solid Phase and
Enzymatic TemplatePreparation
5
Epistatis can becontrasted withdominance, which is
an interactionbetween alleles atthe:
Same gene locus Different gene locus Same allele locus Different allele locus 1
Inheritance of skincolour in humanbeings is an exampleof:
Sex linkedinheritance
Quantitativeinheritance
Sex limited inheritance Qualitative inheritance 2
In which phase ofcell cycle can
euchromatin andheterochromatin bedistinguished?
Interphase Metaphase Prophase Anaphase 1
Which of thefollowing is aconsequence of
overlapping genes?
Increased thetolerance formutation.
Reduced thetolerance formutation.
Trisomy Tetrasomy. 2
Turner syndrome isan example ofmonosomy where an
individual is bornwith:
X Chromosome Y Chromosome XXX Chromosome XXY Chromosome 1
Which of thefollowing is not acause of damage tobases of DNA?
Deamination Depurination Depyramidination Depolymerization 4
Which of thefollowing containsAT richAutonomouslyReplicatingSequences (ARS)?
Bacterial genome Fungal Genome Yeast genome Viral genome 3
In which of thefollowing forms doesDNA exists at arelative humidity of70%?
A-form B-form C-form Z-form 1
Which of thefollowing operonsrequire CataboliteActivator Protein(CAP) forrepression?(i) Lac operon(ii) Trp operon(iv) Arabinose
operon.
Both (ii) and (iii) (i) only (ii) only Both (i) and (iii) 4
Which of thefollowing RNApolymerase helps intranscription oft-RNA and smallRNA?
RNA Pol-I RNA Pol-II RNA Pol-III None of above 3
Which of thefollowing antibioticsinhibits thefunctioning ofprokaryoticribosomes?
Aminoglycosides Chloramphenicol Tetracycline Cycloheximide 1
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The ____________
have long terminalrepeats (LTR's)similar toretroviruses.
V iral Transposons Non-Viral
transposons
DNA transposons RNA transposons 1
Which of thefollowing is not an
intercalating agent?
Ethidium Bromide Daunomycin Doxorubicin Tris Chloride 4
During the synthesisof cDNA, 3'-OH isprovided byformation of a loopby the singlestranded DNA itself.
This loop is furtherreleased by
___________.
EcoRI S1 nuclease RNA polymerase Helicase 2
Which of thefollowing plasmidscontains partitiongenes that promotethe even distributionof plasmids after
bacterial celldivision?
F-plasmid Rec-plasmid GFP-plasmid Col-plasmids 1
Which of thefollowing polymeraseis produced instationery phase andconsidered to be anerror-pronepolymerase?
DNA Polymerase-I DNA Polymerase-II DNA Polymerase-III DNA Polymerase-IV 4
The process ofsequencing of wholegenome in which theDNA is broken intopieces, sequencedand finally, the readsare overlapped andcorrect reads areobtained is known as
___________.
Pyrosequencing Shot-gun method of sequencing
Chromosome walking Dideoxy method of sequencing
2
Which of thefollowing moleculesis not utilized  in pyrosequencingtechnique?
DNA Polymerase Luciferase Apyrase Dideoxynucleotides 4
Which of thefollowing is not asubstrate forglucuronidase?
5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid(X-Gluc)
p-nitrophenyl-beta-D-glucuronide
4-methylumbelliferyl-beta-D-glucuronide (MUG)
Isopropyl-beta-D-1-thiogalactopyranoside(IPTG)
4
Which of thefollowing is anapplication of cDNAlibraries?
Mendelian genetics Reverse genetics Entomology Inheritance studies 2
When conjugation isinitiated by a signal
_______ creates anick in one of thestrands of theconjugative plasmidat the oriT and helpsin transfer of DNA.
Helicase Relaxase Endonuclease Exonuclease 2
In which of thefollowing phasesdoes independentassortment of genesoccur in eukaryotes?
Metaphase-I Anaphase Prophase Telophase 1
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What do the densely
stained and lightlystained areas of anucleus denoteduring the process ofnuclear staining?
Heterochromatin and
Euchromatinrespectively
Euchromatin and
Nucleolusrespectively
Heterochromatin and
Cytoplasm respectively
Cytoplasm and
Nucleolus respectively
1
Which of the
following is true?(A) C rossing overhappens betweentwo non-sisterChromatids.(B) Thecomplementarity ofthe strands aftercrossing over is notpreserved.(C) Holliday Junctionis the trihedralstructure associatedwith crossing over.(D) Crossing overtakes place onlybetween
homologouschromosomes.
Both A and D A,B and C Both B and D B, C and D Both A and C 1
Which of thefollowing is NOT a
DNA damage?
Thymine dimers Pyrimidine dimers Cyclobutane PyrimidineDimers
UV-endonuclease-sensitive-sites
3
Transposition is alsocalled illegimaterecombinationbecause
________________.
It require homologybetween sequences.
It helps in formingnew recombinants.
It is not site specific. It results into death ofthe cell.
3
The meltingtemperature or Tm ishighest for thoseDNAs which containhighest proportion of
______________.
C+A A+T C+G A+T 3
Zinc finger motifcomprises two betastrands and onealpha helical region.Which of these is/areresponsible forinteraction with theDNA?
Beta strands One beta strand andthe alpha helicalregion
The alpha helical region Both the beta strandsand the alpha helixtogether
3
Which of thefollowing requires
TA TA BindingProtein (TBP) forproper initiation oftranscription?
RNA polymerase I RNA polymerase II RNA polymerase III All of the above 3
The relative distancebetween two genescan be calculated bytaking the offspringof an organismshowing two linkedgenetic traits, andfinding thepercentage of theoffsprings where thetwo traits do not runtogether. If thepercentage of thedescendants thatdoes not show bothtraits are higher it
The two genes arecloser to each otheron the chromosome.
The two genes arefar apart to eachother on thechromosome.
The two genes areoverlapping on each otheron the chromosome.
The two genes do notbelong to the samechromosome.
2
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means that:
Bacteriophages carryportions of DNAfrom one bacteria toanother in a processcalled:
Transformation Transfection Transduction Conjugation 3
Which of the
following is theinitiation codon?
AUG CUG AUC AGU 1
The complementaryDNA strand for5'-TACGATCATAT-3'would be:
3'-TA CGA TCA TA T-5' 3'-A TGCTA GTA TA -5' 3'-A UGCUA GUA UA -5' 3'-GCA TA TA CGCG-5' 3'-TA TA CTA GCA T-5' 2
Which of thefollowing is not truefor house keepinggenes?
Constitutiveeuchromatin that isalways turned on.
Constitutiveheterochromatin thatis always turned on.
Constitutive euchromatinthat is always turned off.
Constitutiveheterochromatin that isalways turned off.
1
The first amino acidincorporated in aprotein chain ofeukaryotes andprokaryotes is:
Histidine Alanine Cysteine Methionine 4
How does the stranddirected mismatchrepair systemdistinguish the newDNA strand from theold one?
Hydroxyl groups areadded to all Aresidues in thesequence GATCsome time after theA has beenincorporated into anewly synthesizedDNA chain.
Methyl groups areadded to all Aresidues in thesequence GATCsome time after theA has beenincorporated into anewly synthesizedDNA chain.
Phosphate groups areadded to all A residues inthe sequence GATC sometime after the A has beenincorporated into a newlysynthesized DNA chain.
Ethyl groups are addedto all A residues in thesequence GATC sometime after the A hasbeen incorporated intoa newly synthesizedDNA chain.
2
What is the function
of DNAtopoisomerasesduring DNAreplication?
They catalyze
nucleosidetriphosphatepolymerization.
They help in opening
up the DNA helix sothat it can be copied.
They seal together the
discontinuouslysynthesized lagging-strandDNA fragments.
They help to relieve
helical winding and toprevent DNA tangling.
4
Which of the
following is not acomponent of anucleosome?
DNA segment Histone protein Chromatin Ribosomes 3
What is the role ofthe cAMP-CAPcomplex in E. coli?
It binds to theoperator, inhibitingtranscriptioninitiation.
It binds to the CAPsite, where itinteracts with RNApolymerase to inhibittranscriptioninitiation.
It binds to the operator tostimulate the rate oftranscription initiation.
It binds to the CAP site,where it interacts withRNA polymerase tostimulate the rate oftranscription initiation.
4
The mechanism ofpre-mRNA splicingimplies that thesplicing machinerymust recognize
_______ of theprecursor RNAmolecule.
the 5' splice site andthe 3' splice site
the 5' splice site andthe branch point
the 3' splice site and thebranch point
the 5' splice site, the 3'splice site and thebranch point
4
Which of thefollowing statementsis true regardingeukaryotic mRNA?
Eukaryotic mRNA  is essentiallymature upontranscription and
requires noprocessing.
Eukaryotic translationis coupled totranscription andoccurs
co-transcriptionally.
Introns are   presentin the mRNA of alleukaryotes.
Both ends of eukaryoticmRNAs are modified bycapping on the 5' endand by polyadenylation
of the 3' end.
4
Which of thefollowing statementsis false regardingcodons?
Most amino acids arerepresented by morethan one codon.
Codons for the sameamino acid tend tocontain the samenucleotides at thefirst and secondpositions.
Three codons act asinitiation sites signaling thestart of the protein-codingsequence.
The tRNA moleculebinds to the codonduring translation.
3
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The two subunits of
the ribosome jointogether on the
________ , usuallynear its _________end, to ini tiate thesynthesis of aprotein.
mRNA molecule, 5' rRNA molecule, 5' mRNA molecule, 3' rRNA molecule, 3' 1
How doestetracycline inhibi tthe protein synthesisin bacteria?
It binds in the exitchannel of theribosome andthereby inhibitselongation of thepeptide chain.
It blocks the peptidyltransferase reactionon ribosomes.
It blocks binding ofaminoacyl-tRNA to A -siteof ribosome.
It blocks thetranslocation reactionon ribosomes.
3
Which of thefollowing statementsis false regarding the
use of transposableelements in geneticresearch?
They are naturallyoccurring mutagens.
They function asDNA mismatch repairsystems.
They act as cloning tags. They function asvehicles for introducingforeign DNA into model
organisms.
2
Insertions ordeletions withincoding regions may
lead to___________ andprematureterminationof___________.
Missense mutation;transcription
Frameshift mutation;translation
Missense mutation;translation
Frameshift mutation;transcription
2
What feature(s)is/are required for aDNA molecule toserve as a vector?(i) It must be able toindependentlyreplicate itself andthe fragment itcarries.(ii) It should carry aselectable marker
gene.(iii) It should containseveral recognitionsequences.(iv) It should be easy
to recover from thehost cell.
(i), (ii) and (iv) (ii), (iii), and (iv) Both (i) and (iii) Both (i) and (ii) All - (i), (ii), (iii) and(iv)
5
pUC18 plasmidcarries a fragment ofthe bacterial lacZgene, and thepolylinker is insertedinto this fragment. Ifa DNA fragment isinserted into thepolylinker, the
bacterial cell carryingthe plasmid forms________ colonieswhen grown onmedium containing
______ .
Blue, X-Gal White, X-Gal Blue, X-Lac White, X-Lac 2
Which of thefollowing statementsis true aboutcosmids?
They are also calledphagemids.
They can carry DNAinserts up to 15 kb inlength.
They are plasmid vectors. They contain asequence of phagelambda.
4
What is a knockoutmouse?
It contains multiplecopies of a humangene.
It lacks a knownnormal gene.
It contains one normal andone mutant copy of aparticular gene.
It contains a genewhich is over expressed
2
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How can a specific
clone be identified ina DNA library?
By gene targeting. By colony
hybridization.
By recombinant DNA
technology.
By DNA hybridization. 2
___________ is theprocess by which ahost organism cantake up DNA from its
environment. E. colican take up DNAafter treatment with
_________.
Transformation,sodium ions
Transformation,calcium ions
Transvection, sodium ions Transvection, calciumions
2
What is theprobability that aplant of genotypeAaBb will beproduced fromparental plants of the
genotypes AaBb andAabb if the two genepairs independentlyassort during gameteformation?
0.5 0.25 0.125 1 2
The following are
frequencies ofcrossing overbetween each pair ofthree genes: A and B0.5 %; B and C 34.5%, A and C 35.4 %.What is the order ofthe genes on thechromosome?
A-B-C B-A-C A-C-B B-C-A 1
How many possiblereading frames mustbe examined insearching for ORFs(Open ReadingFrames) in anunidentified DNA
sequence?
1 2 3 6 4
Which of thefollowing convertssingle stranded DNAto double strandedDNA during cDNAsynthesis?
DNA Polymerase Nuclease RNase Reverse Transcriptase 1
Infection of the samecell by two viruses,in which oneprovides a geneproduct which theother requires iscalled ___________.
Anabolism Canabolism Complementation Symbiosis 3
The endonucleases
which cut DNAmolecules at a  particular pointwithin a specificregion of six basepairs are called
______________.
Site specific
nucleases
Sequence specific
nucleases
Base specific Amino acid specific 2
The cofactorrequired for E.coliDNA ligase is:
NAD+ ATP NADPH AMP 2
The best example forprocessive enzyme inprokaryote DNAsynthesis is:
DNA Polymerase III DNA Polymerase IV DNA Polymerase II DNA Polymerase I 1
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7/30/2019 Interview Questions - Set 2
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Choose the correct option. / Fill in the blanks.
Question Ans 1 Ans 2 Ans 3 Ans 4 Ans 5 CorrectAnswer
A fragment of
synthetic DNAcontaining arestriction site thatmay be used forcloning of genes iscalled:
Adaptors Linkers Regulator Homopolymeric tail 2
If a mutation is fixedin the population,those genes arecalled:
Unitary pseudogenes Duplicated genes Processed genes Overlapping genes 1
__________ alterthe specificity of  RNApolymerase for agiven promoter inprokaryotic
transcription.
Gamma factors Sigma factors Delta factors Alpha factors 2
Enhancement ofinteraction betweenRNA polymerase & aparticular promoteris done by:
Activators Enhancers Repressors General Transcriptionfactors
1
The large fraction ofthe C-value ineukaryotic cell ismade by:
Tranposons Insertinal elements Nucleases Mobile genetic elements 1
The recombinantfrequency of 1% isequivalent to:
1 centimorgan 10 centimorgans 5 centimorgans 100 centimorgans 1
A genetic map is amap based on thefrequencies ofrecombinationbetween markersduring:
Meiosis Mitosis Crossing over Replication 1
___________disease is anexample forMendelian trait.
Mulberry HeartDisease
Sickle-cell anemia Xerophthalmia Scurvy 2
Mating the F1 hybrid
with one of theparent is called
__________.
Test cross Back cross Three-point cross Two-point cross 2
Misalignment ofbases in DNAreplication is notcaused by:
Acridine molecules Alkylating agents Base analogs Nitrous acid 1
Mueller inducedlethal mutation inDrosophila by
treating with:
Gamma rays X-rays IR rays Alpha rays 2
Maxam-Gilbertmethod involves
_________modification of DNA .
Chemical Enzymatic Radioactive Flourescent 1
In which of thefollowing sequencingmethodreversible-dyeterminators areused?
Solid Illumina Ion semiconductor Pyrosequencing 2
In which of thefollowing methods ofsequencing theenzyme luciferase is
Automatedsequencing
Pyrosequencing Sangers dideoxy method Maxam-Gilbert method 2
INTERVIEW QUESTIONS - SET 2
13 of 15
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7/30/2019 Interview Questions - Set 2
14/15
Choose the correct option. / Fill in the blanks.
Question Ans 1 Ans 2 Ans 3 Ans 4 Ans 5 CorrectAnswer
used?
In which of thefollowing sequencingmethods thetechnique ofchromosome walkingis observed?
Shotgun Sequencing Maxam-Gilbertmethod
Automated sequencing Sangers dideoxymethod
1
The reporter gene,which causes cellsthat express it toglow green underblue light, is called:
GUS GFP Luciferin YFP 2
A tRNA attached toits amino acid iscalled
_____________.
Uncharged tRNA Charged tRNA Neutralized tRNA Close ended tRNA 2
Which amino aciddoes the IUBMBsymbol "L" indicate?
Phenylalanine Histidine Leucine Lysine 3
The enzyme involvedin the attachment ofcorrect amino acid to
each type of tRNA is___________.
Aminoacyl-tRNAsynthetase
DNA Polymerase RNA Polymerase Aminoacyl-tRNA ligase 1
Which of thefollowing is stopcodon amber?
UAA UGA UAG UGG 3
Which of thefollowing is a featureof Prokaryoticgenome?
It is usually circularand tangled duringthe cell growthphase.
It is found in thenucleoid region.
It is highly extended andtangled during celldivision.
It is compressed andfolded.
3
What is a ribozyme? RNA without sugarwhich is foundprokaryoticorganisms.
RNA with extraphosphate.
RNA with enzymaticproperty.
RNA with catalyticproperty which is foundonly in the nucleus ofeukaryotic organisms.
3
Which of the
following reactions isNOT a posttranslationalmodification?
Hydroxylation Glycosylation Phosphorylation Polyadenylation 4
What would IPTG doif it is added to aculture of bacteriagrowing on poorlyutilized carbonsource?
It would bringconformationalchange in thestructure of inducermolecule.
It would induce thelac operon enzymes.
It would bringconformational change inthe structure of repressormolecule.
It would repress the lacoperon enzymes.
3
GACNNN/NNGTC isthe restriction site forAspEI and AhdI.
Therefore, the twoenzymes can beknown as:
Neoschizomers Isoschizomers Isocaudomer Blunt-end isomers 2
Aat II cuts at(GACGT/C) and Zra Icuts at (GAC/GTC).
Therefore, they canbe known as:
Isocaudomers Neoschizomers Isoschizomers Blunt-end isomers 2
Which of thefollowing is used asa tag for bases inautomated DNAsequencing?
Radioactive tag Fluorescent tag Antibodies Tracer dyes 2
What areneoschizomers?
Same restrictionenzymes thatrecognize differentsequences.
Different restrictionenzymes thatrecognize differentsequences.
Different restrictionenzymes that recognize thesame sequence.
Same restrictionenzymes that recognizesame sequences.
3
INTERVIEW QUESTIONS - SET 2
14 of 15
-
7/30/2019 Interview Questions - Set 2
15/15
Choose the correct option. / Fill in the blanks.
Question Ans 1 Ans 2 Ans 3 Ans 4 Ans 5 CorrectAnswer
At which of the
following positionsdoes doublestranded LambdaDNA has (a) smallsingle strandedregion(s)?
3' end 5' end It does not have a single
stranded region.
In the middle of the
DNA sequence.
2
Which of thefollowing is true forpreproteins?
They needposttranslationalmodifications tobecome active.
They are proteinswithout signalpeptide.
They need posttranscriptionalmodifications.
They need bothpost-translational andpost-transcriptionalmodifications.
1
Which of thefollowing is NOT agene family?
Homeobox Achaete-scutecomplex
Major histocompatibilitycomplex (MHC)
All of the above belongto gene family.
4
Which of thefollowing is true for
Rifampicin?
It inhibits DNApolymerase.
It preventstranscription of DNA
to RNA.
It is lipophobic in nature. It allows transcriptionbut inhibits at the
translation.
2
Which among thefollowing screeningprocedure is highlysuited to findadjacent genomic
clones from alibrary?
Hybrid arrest Colony and plaquehybridization
Chromosome walking Primer walking 3
Transgenicorganisms are:
The ones thatcontain a foreign
gene and extracopies ofendogenous gene.
Geneticallymanipulated
organisms thatcontain multipleforeign genes.
Genetically manipulatedorganisms that contain
multiple foreign genes,extra copies ofendogenous gene.
The ones that contain acopy of endogenous
gene and multiplecopies of foreign genes.
3
Which of thefollowing is true fora retrotransposon?
It has a structuresimilar to retroviralgenome.
It encodes proteinssimilar to retroviralreverse transcriptase.
It is flanked by longterminal repeatsequences(LTR).
It is a genetic elementwhich can amplify itselfon a genome.
4
Which of thefollowing statementsis false regardingsatellite DNA?
Prokaryotes aredevoid of it.
It forms a variableproportion of thetotal DNA,depending on thespecies.
It is composed of shortrepetitive sequences.
It is found in theeuchromatic regions ofchromosomes.
4
Which of thefollowing statementsis true about pairedend sequencing?(i) Paired endsequencing is alsocalled double barrelshotgun sequencing.(ii) Paired endsequencing is usedto overcome thedifficulty of havingrepetitive DNAsequences.(iii) Paired endsequencing permitsthe assembly of largegenome scaffolds.
(i) and (ii) (ii) and (iii) (iii) only (i) only (i), (ii) and (iii) 5
_____________ isthe process ofsecondary antibodylinked to an enzyme.
Biotransformation Bioconjugation Biotransduction Biotransfusion 2
The proteinbeta-galactosidase isencoded by a genecalled
____________.
lacI lacY lacZ lacA 3
INTERVIEW QUESTIONS - SET 2