INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP) … · 2013. 10. 9. · CWOOM9 codeine, morphine,...
Transcript of INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP) … · 2013. 10. 9. · CWOOM9 codeine, morphine,...
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INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)
INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
Summary ReportBIOLOGICAL SPECIMENS
2013/1
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Samples Substances Concentrations Comments on substancesSample 1 Morphine (Total) 2300 ng/ml
6-Monoacetylmorphine (6-MAM) 576 ng/ml
Codeine 1150 ng/ml
Sample 2 Nordazepam 1727 ng/ml
Oxazepam 457 ng/ml
Amfetamine 1143 ng/ml
Temazepam 464 ng/ml
Sample 3 gamma-Hydroxybutyric acid (GHB) 14427 ng/ml
Sample 4 [blank sample]
Test Samples Information
Samples Comments on samplesSample 1 To prepare BS-1, urine was spiked with Morphine sulphate (3057ng/ml, 2300ng base/ml), Codeine
(1150ng base/ml) and 6-Monoacetylmorphine (576 ng base/ml). The spiked urine was dispensed in 50ml aliquots and lyophilised.
Sample 2 To prepare BS-2, urine was spiked with Amfetamine sulfate (1562ng/ml, 1143ng base/ml), Nordazepam (1727ng/ml), Oxazepam (457ng/ml) and Temazepam (464ng/ml). The spiked urine was dispensed in 50ml aliquots and lyophilised.
Sample 3 To prepare BS-3, urine was spiked with Sodium gammahydroxybutyrate (14427ng GHB/ml, ). The spiked urine was dispensed in 50ml aliquots and lyophilised
Sample 4 BS-4 was a blank test sample prepared from urine containing no substance in the ICE menu. The sample contained urine spiked with Ephedrine hydrochloride (2700ng/ml, 2308ng base/ml). The spiked urine was dispensed in 50ml aliquots and lyophilised.
2013/1-BS Copyright (c) 2013 UNODC(2)
INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
Table of contents Introduction Page 3 Comments from the International Panel of Forensic Experts Page 3
Codes and Abbreviations Page 4 Sample 1 Analysis Page 5 Identified substances Page 5
Statement of findings Page 7 Identification methods Page 11
Summary Page 13 Z-Scores Page 14 Sample 2 Analysis Page 18 Identified substances Page 18
Statement of findings Page 20 Identification methods Page 24
Summary Page 26 Z-Scores Page 27
Sample 3 Analysis Page 33 Identified substances Page 33
Statement of findings Page 34 Identification methods Page 38 Summary Page 40
Z-Scores Page 41
Sample 4 Analysis Page 43 Identified substances Page 43
Statement of findings Page 44 Identification methods Page 48 Summary Page 50
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Introduction
An important element of the UNODC International Quality Assurance Programme (IQAP) is the implementation of the International Collaborative Exercises (ICE). The exercises allow laboratories, from both developing and developed countries, to continuously monitor their performance in drug testing on a truly global scale. Two rounds are offered per year with each round presenting participants with four different test samples for analysis.
This report provides information on the analytical results of laboratories participating in the Biological Specimens (BS) group. In order to maintain confidentiality, the participating laboratories have been assigned random “Web Codes”, which change every round. The analytical results returned by laboratories participating in ICE are evaluated by UNODC and a confidential report is provided to each laboratory on its own performance. The overall analytical results are reviewed by the UNODC’s International Panel of Forensic Experts which oversees the implementation of these exercises, and offers guidance and support in addressing relevant quality issues. The exercises provide an overview of the performance and capacity of participating laboratories and enable UNODC to tailor technical support in the laboratory sector for greatest impact.
Comments from the International Panel of Forensic Experts
Participation of laboratories
Samples within both the Seized materials (SM) and Biological Specimens (BS) test group were sent to 167 laboratories in 57 countries. 68 laboratories participated in the BS test group and 58 (85%) laboratories submitted the results of their analysis in time for inclusion in the summary report, with 96% using the ICE portal for return of results. The almost universal usage of the ICE portal continues to be a high endorsement of its utility.
Qualitative and Quantitative Analysis of the biological specimens test samples
Overall, the level of performance in identification of the test samples was good given the inherently higher level of difficulty in the analysis of biological specimens compared to seized materials. The complexity of the test samples in ICE 2013/1 was increased in order to challenge the participants. BS-1 contained morphine, 6-monoacetylmorphine and codeine, which were identified by 79%, 79% and 91% of participants respectively. It is encouraging to note that 71% of participants identified all three components in BS-1 and only five false positive results were reported. BS-2 contained amphetamine and a mixture of three benzodiazepines (nordazepam, oxazepam and temazepam). While only 42% of participants identified all four components, 86% identified at least one of the benzodiazepines and 65% identified the amphetamine present. There were eight false positive results including four for diazepam. While the benzodiazepines present are metabolites of diazepam, their presence alone does not represent use of diazepam. BS-3 contained gammahydroxybutyric acid (GHB), a new substance on the ICE menu for the BS test group and 22% of participants identified its presence, while 64% of participants did not carry out analysis for identification of GHB. It is recognized that while GHB may not be commonly analysed by all participants, it has been included in the ICE menu as it represents an example of a more recently abused substance and at the Commission on Narcotic Drugs in March 2013, GHB was transferred from schedule IV to schedule II of the Convention of Psychotropic Substances of 1971. BS-4 was a blank test sample containing no substances from the ICE menu. Although it did contain ephedrine, a precursor to methamphetamine. 19% of participants identified the ephedrine and among five false positives, there was one for methamphetamine.
The results for quantitative analysis within the BS test group were excellent. For BS-1 24 out of 25 (96%) laboratories quantified the morphine, 21 out of 22 (95%) quantified 6-MAM and 22 out of 23 (96%) quantified codeine with acceptable Z-scores. Within BS-2, the quantitation results were equally as good with 20 of 21 (95%) laboratories quantifying the amfetamine, 20 out of 22 (91%) the nordazepam, 13 of 16 (81%) the oxazepam and 14 out of 16 (88%) the temazepam with acceptable Z-scores. Within BS-3, 7 out of 9 (87%) participants quantified the GHB present with acceptable Z-scores. It is encouraging to note that of those laboratories who did carry out quantification, 52% performed quantification on at least seven of the eight substances in all test samples. The predominant analytical techniques used by participants for quantification within the BS test group were GC/MS (79%), followed by LC/MS (38%) and others at (17%).
Laboratories are encouraged to carry out quantification as it can improve the quality of laboratory analyses and can be helpful in assessing the significance of the results. Also, laboratories reporting false positive or false negative results should investigate the reasons for this and corrective actions should be taken in order to continuously improve performance. Participation in ICE also helps in monitoring the effect of corrective actions.
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Codes and Abbreviations
Tech Code Name100 Agglutination Techniques
110 Enzyme Immunoassay Techniques
120 Fluorescence Polarization Immunoassay
130 Radioimmunoassay
140 Colorimetric reactions
141 Marquis reagent (sulphuric acid, formaldehyde)
142 Cobalt thiocyanate
150 Thin Layer Chromatography
160 High/Ultra High Performance Liquid Chromatography
161 High Performance Liquid Chromatography with diode array detection
170 Gas Chromatography NPD
171 Gas Chromatography FID
172 Gas Chromatography ECD
180 Gas Chromatography/Mass Spectrometry
190 Fourier Transform Infrared Spectrometry
200 Spectrophotometry (visible, UV)
210 Others (specify)
211 NMR
220 Microcrystal test
230 Liquid chromatography/mass spectrometry
231 Liquid chromatography/tandem mass spectrometry
(+) “positive”: Indicates that the analyte is identified; for presumptive tests (e.g. colour reactions), indicates that a positive reaction was obtained.
(-) “negative”: Indicates that the analyte is not identified.
(ANP): Analysis not performed.
2013/1-BS Copyright (c) 2013 UNODC(4)
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Identified substances for Test Sample 1
Code Identified Substances0709WO 6-MAM, Morphine, Codeine
1C2WN2 opiates
1M39MI codeine, morphine, 6-monoacetylmorphine
2I1J7W codeine, 6 MAM and morphine
3ESBDT CODEINE, ACETYLMORPHINE
5A0LTW morphine, 6-MAM, codeine
5D8EIT Morphine, Heroin and Codeine
6H33U3 Codeine and 6-MAM
6ZZZCD morphine,codeine, 6-monoacethylmorphine
76JL37 morphine, 6-MAM, codeine
7HEM7O Codeine
98KDM8 Morphine, codeine and monoacetly-morphine
ABBR1D Morphine,O6-monoacetylmorphine,codeine
AKQ21O morphine, codeine and 6-acetyl-morphine
ANXWGY Codeine, morphine and 6-Monoacethylmorphine
BBRB0E Morphine
BJG4OO 6-MAM, codeine, morphine
BPL9ZY morphine, 6-MAM, codeine
BVABMB 6-MAM, codeine, morphine
CUH0QQ CODEINE, MORPHINE AND MORPHINE-6-ACETYL.
CWOOM9 codeine, morphine, 6-mam
CZJD27 morphine, codeine
D7TTWV OPIÁCEOS
DONO2Y 6-MAM, codeine, morphine
ERWOWW Codeine
FNSUKR 6-monoacetilmorfina, morfina, codeina
ILS9UP Codeine, Morphine, 6-Monoacetylmorphine
J2FAYZ opiates
J6WWIX Heroin abuse
JBUJJA Codeine
JLCQ3D CODEINE
JWS3VW morphine, codeine, 6-mam
JZ6JTH Amphetamine
KHMLHA Morphine, Codeine, 6-monoacetylmorphine.
KKPG9K 6-Monoacetylmorphine, Morphine and Codeine
LFLTTZ Opiates
M8E6T6 morphine, codeine, 6-monoacetylomorphine
NDS3R3 codeine, morphine, monoacetylmorphine
OOOQSO 6-monoacetylmorphine, morphine, codeine
PSXQYE Morphine, Codeine, 6-MAM
Sample 1 Analysis
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Code Identified SubstancesQHGM5Q Morphine, Codeine, 6-MAM
RFGGQG codeine, morphine, 6-monoacetylmorphine
SAV6NX 6MAM, Morphine and codeine
TTAVXV Morphine, codeine and 6-monoacetyl-morphine
UM0M1S Codeine, Morphine, 6-MAM
VHDEKU Codeine+morphine+6MAM
WIYO85 Opiates
WWSTWW morphine, codeine and 6-monoacetylmorphine
XA9AAA Morfine - Codeine - 6-MAM
XDXVS9 Codeine and 6-Monoacetylmorphine
XJZEEE Codeine, Morphine, 6-MA
XLEICI CODEINE
XTLXKX Morphine, Codeine, 6-Monoacetylmorphine
XXHQNV Morphine and 6-MAM
YCEET4 cideine,MAM, morphine
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Code 6-Monoacetylmorphine (6-MAM)
Codeine Morphine (Total)
0709WO + + +-
1C2WN2 + + +morphinecodeine6-monoacetylmorphinecaffeine
1M39MI + + +sample positive for opiates
2I1J7W + + +
3ESBDT + + ANPCodeine and Acetylmorphine were detected.
5A0LTW + + +
5D8EIT + + -The sample contain: 2560 ng/mL of morphine, 1200 ng/ml of coedine. Heroin and caffeine was also detected.
6H33U3 + + ANPCodeine and 6-MAM was detected in sample BS1 but quantification analysis was not performed.
6ZZZCD + + +2013/1/BS-1 urine sample contain morphine,codine,6-monoacethylmorphine(heroine user)
76JL37 + + +Findings indicate that the person consumed heroin.
7HEM7O - + -
98KDM8 + + +The urine was found to contain morphine, codeine and monoacetyl-morphine.
ABBR1D + + +
AKQ21O + + +The presence of morphine, codeine and 6-acetyl-morphine indicates the use of heroin.
ANXWGY + + +Codeine, morphine and 6-Monoacethylmorphine detected
Statement of findings for Test Sample 1
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Code 6-Monoacetylmorphine (6-MAM)
Codeine Morphine (Total)
BBRB0E ANP + +we found morphine in BS-1 as a controlled substance
BJG4OO + + +There was heroine consumption
BPL9ZY + + +We also found caffeine in BS-1.
BVABMB + + +sample positive to 6 MAM: 425.2 ng/ml (cut-off 100 ng/ml)sample positive to codeine:591.3 ng/ml (cut-off 100 ng/ml)sample positive to total morphine:1845.3 ng/ml (cut-off 100 ng/ml)
CUH0QQ + + +IN THE ROUND AS LABELED "ROUND ICE 2013/1 SAMPLE BS-1" DETECTED CODEINE, MORPHINE AND MORPHINE-6-ACETYL.
CUUFFQ + + +
CWOOM9 + + +Compatible with consum of heroin
CZJD27 ANP + +Test sample contains morphine and codeine at 2739.84 ng/mL and 1199.05 ng/mL respectively using GCMS
D7TTWV ANP ANP ANPPOSITIVO (PRESUNTIVO)
DONO2Y + + +6-MAM was detected in urine. Results indicate the previous use of heroin, a controlled narcotic (illegal).
The concentration of GHB reflect the physiological concentrations.ERWOWW - + -
Codeine was identified by GC/MS
FNSUKR + + +La coincidencia entre los tiempos de retención y fragmentogramas de una muestra biológica analizada en las mismas condiciones de un estandar indica la presencia de 6 monoacetilmorfina, morfina y codeína en esta.
FZZZZQ ANP ANP ANP
ILS9UP + + +Codeine, Morphine, 6-Monoacetylmorphine
J2FAYZ + + +we have not received standards
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Code 6-Monoacetylmorphine (6-MAM)
Codeine Morphine (Total)
J6WWIX + + +Presence of 6-MAM
JBUJJA - + +free
JLCQ3D - + -The sample BS-1 contain CODEINE
JWS3VW + + +
JZ6JTH - - -
KHMLHA + + +In the urine sample analyzed, was detected and confirmed the presence of Morphine, Codeine, 6-monoacetylmorphine.
KKPG9K + + +The sample contains 6-Monoacetylmorphine, morphine and codeine.
LFLTTZ - + +Codeine, Morphine and metabolites
M8E6T6 + + +use of opiates
MDKW7J + + +
NDS3R3 + + +consumption of diacetylmorphine
OOOQSO + + +
PSXQYE + + +Sample is positive for Morphine, Codeine and 6-MAM
QHGM5Q + + +
RFGGQG + + +sample positive for opiates
SAV6NX + + +Morphine and codeine are metabolites of 6MAM. Presence of 6MAM indicates a heroin consumption.6MAM, Morphine and codeine are controlled narcotics in France
2013/1-BS Copyright (c) 2013 UNODC(9)
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Code 6-Monoacetylmorphine (6-MAM)
Codeine Morphine (Total)
TTAVXV + + +The 6-monoacetylmorphine detection indicates heroin consumption, codeine could bepresent as heroin impurity or could come from the intake of the drug itself. Finally,morphine could come from the heroin or codeine consumption or could come from theintake of the drug itself.
UM0M1S + + +Screening by immunoassay technich : PositiveConformation by GC-MS : Positive
VHDEKU + + +Sample containing morphine+codeine+6MAM which indivates consumation of heroine
WIYO85 + - +BS1positive for: Morphine & 6MAM
WWSTWW + + +
XA9AAA + + +Codeine was consumed, Morphine indicates an opiate was consumed and 6-MAM marker of Heroin indicate this opiate was consumed.
XDXVS9 + + ANPOn analysis, the sample of 2013/1/BS-1 found to contain Codeine and 6-Monoacetylmorphine.
XJZEEE ANP ANP ANPFree morphine was measured.
XLEICI - + -Codeine was found in BS-1. Quantitation was not performed.
XTLXKX + + +Heroine abuse.
XXHQNV + + +Morphine and 6-MAM are metabolites of Heroin
YCEET4 + + +
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Identification methods for Test Sample 1
Code 110 120 150 161 170 171 180 230 231 210
0709WO SI
1C2WN2 S I I
1M39MI S SI
2I1J7W S I I
3ESBDT S I I
5A0LTW S I I
5D8EIT S I
6H33U3 I
6ZZZCD S I
76JL37 S I I
7HEM7O S I I
98KDM8 S I
ABBR1D S I I
AKQ21O S I SI I
ANXWGY S I
BBRB0E S I
BJG4OO S I
BPL9ZY S SI SI
BVABMB SI
CUH0QQ S I
CUUFFQ S I
CWOOM9 S I I
CZJD27 I I S DT kits
D7TTWV S
DONO2Y S I I
ERWOWW
S I
FNSUKR S I
FZZZZQ SI
ILS9UP S I I S Kinetic Interactions of microparticles
J2FAYZ I I immunochem
J6WWIX S I I
JBUJJA S I I I
JLCQ3D SI I
Analytical Technique Codes
Legend: S ... used for Screening I ... used for Identification SI ... used for both
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Code 110 120 150 161 170 171 180 230 231 210
JWS3VW S I
JZ6JTH S I I
KHMLHA S I
KKPG9K SI S Multi-Drug Screen Test Panel
LFLTTZ I S Dipromed Drulab screen
M8E6T6 S I
MDKW7J SI I
NDS3R3 S S I
OOOQSO S I
PSXQYE S S I I
QHGM5Q I I SI I
RFGGQG S SI
SAV6NX S I I
TTAVXV S I I I I
UM0M1S S I
VHDEKU SI I
WIYO85 S I
WWSTWW
S I I
XA9AAA S I S Lateral Flow Immunoassay
XDXVS9 I SI
XLEICI S I
XTLXKX S I I
XXHQNV S I
YCEET4 I
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DONO2Y gamma-Hydroxybutyric acid 180
J6WWIX Methadone 180
JZ6JTH Amfetamine
2013/1-BS Copyright (c) 2013 UNODC(13)
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110 120
Response Summary Participants: 54
Frequency of use of screening and identification techniques:
H
Summary for test sample 1The response summary of all 54 participating laboratories for the identification of BS-1, frequency of use of screening andidentification techniques and false positives and negatives:
Identification of test sample 1:
False positives for Test Sample 1
Response SummaryParticipants: 58
6-Monoacetylmorphine (6-MAM): 46
Codeine: 53
Morphine (Total): 46
Code Name Technique
230
5D8EIT Morphine-3-glucuronide 180
J2FAYZ Methadone 180
Section 110 120 150 161 170 171 180 230 231 210
Screening 36 8 4 0 0 0 9 1 1 4
62.1% 13.8% 6.9% 0.0% 0.0% 0.0% 15.5% 1.7% 1.7% 6.9%
Identification 4 0 3 11 2 2 49 5 12 2
6.9% 0.0% 5.2% 19.0% 3.4% 3.4% 84.5% 8.6% 20.7% 3.4%
# labs using this technique
36 8 7 11 2 2 49 6 12 662.1% 13.8% 12.1% 19.0% 3.4% 3.4% 84.5% 10.3% 20.7% 10.3%
Response Summary Participants: 58
False negatives for BS-1(techinque(s) used or ANP) . Within BS-1, there were: 12 false negatives for Morphine 5 false negatives for Codeine 12 false negatives for 6-Monoacetylmorphine
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Z-Scores for Test Sample 1
CODE
6-Monoacetylmorphine (6-MAM)MAD: 100.0
CodeineMAD: 119.5
Morphine (Total)MAD: 440.4
Conc. * AbsDev ZScore Conc. * AbsDev ZScore Conc. * AbsDev ZScore
0709WO 500.0 52.5 0.4 1100.0 34.0 0.2 2750.0 477.5 0.7
5A0LTW 690.0 137.5 0.9 1020.0 46.0 0.3 2540.0 267.5 0.4
5D8EIT 1200.0 134.0 0.8 2560.0 287.5 0.4
76JL37 436.0 116.5 0.8 1268.0 202.0 1.1 2300.0 27.5 0.0
ABBR1D 480.0 72.5 0.5 960.0 106.0 0.6 2239.0 33.5 0.1
BJG4OO 4119.0 3566.5 24.1 21619.0 20553.0 116.1 34982.0 32709.5 50.1
BPL9ZY 626.0 73.5 0.5 1270.0 204.0 1.2 2406.7 134.2 0.2
BVABMB 425.2 127.3 0.9 591.3 474.7 2.7 1845.3 427.2 0.7
CWOOM9 420.0 132.5 0.9 990.0 76.0 0.4 1950.0 322.5 0.5
CZJD27 1199.1 133.1 0.8 2739.8 467.3 0.7
DONO2Y 556.0 3.5 0.0 1121.0 55.0 0.3 1722.0 550.5 0.8
ILS9UP 647.0 94.5 0.6 1063.0 3.0 0.0 2472.0 199.5 0.3
J2FAYZ 575.0 22.5 0.2 1311.0 245.0 1.4 1555.0 717.5 1.1
J6WWIX 510.0 42.5 0.3 930.0 136.0 0.8 1818.0 454.5 0.7
KKPG9K 549.0 3.5 0.0 1069.0 3.0 0.0 2878.0 605.5 0.9
LFLTTZ 1000.0 1272.5 2.0
M8E6T6 650.0 97.5 0.7 1545.0 479.0 2.7 2245.0 27.5 0.0
MDKW7J 590.0 37.5 0.3 1300.0 234.0 1.3 2000.0 272.5 0.4
PSXQYE 705.0 152.5 1.0 1063.0 3.0 0.0 2508.0 235.5 0.4
QHGM5Q 450.0 102.5 0.7 900.0 166.0 0.9 1980.0 292.5 0.4
SAV6NX 730.0 177.5 1.2 1321.0 255.0 1.4 3319.0 1046.5 1.6
WIYO85 578.0 25.5 0.2 1510.0 762.5 1.2
WWSTWW 500.0 52.5 0.4 1220.0 154.0 0.9 2240.0 32.5 0.0
XJZEEE 769.0 216.5 1.5 984.0 82.0 0.5 2629.0 356.5 0.5
XTLXKX 580.0 27.5 0.2 974.0 92.0 0.5 2726.0 453.5 0.7
*) Concentration: ng/ml
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
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Substance: BS-1/6-Monoacetylmorphine (6-MAM) 100MAD =Median = 552.5
Round: 2013/1
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
Z-Score Report per Sample per Substance
UNODC value = 576
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Substance: BS-1/Codeine 120MAD =Median = 1066
Round: 2013/1
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
Z-Score Report per Sample per Substance
UNODC value = 1150
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Substance: BS-1/Morphine (Total) 440.4MAD =Median = 2272.5
Round: 2013/1
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
Z-Score Report per Sample per Substance
UNODC value = 2300
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Identified substances for Test Sample 2
Code Identified Substances0709WO Amfetamine, Nordiazepam, Oxazepam, Temazepam
1C2WN2 amphetamines
1M39MI oxazepam, nordiazepam, temazepam and amphetamine
2I1J7W nordazepam, oxazeam, temazepam and amfetamine
3ESBDT Nordazepam
5A0LTW nordazepam, temazepam, oxazepam, amfetamine
5D8EIT Nordazepam, oxazepam, temazepam, theobromine
6H33U3 Nordazepam and Temazepam
6ZZZCD nordazepam,methamphetamine(traces)
76JL37 amfetamine, nordazepam, temazepam, oxazepam
7HEM7O Nordazepam
98KDM8 Nordiazepam and amphetmaine
ABBR1D Amphetamine,Nordazepam,Oxazepam,Temazepam
AKQ21O amphetamine, nordiazepam, temazepam and oxazepam
ANXWGY Nordazepam and temazepam
BBRB0E amphetamine
BJG4OO temazepam, nordazepam
BPL9ZY diazepam, nordazepam,oxazepam, temazepam
BVABMB amphetamine, oxazepam, nordiazepam, temazepam
CUH0QQ AMPHETAMINE, NORDIAZEPAM, OXAZEPAM AND DIAZEPAM
CWOOM9 Amfetamine, nordazepam
CZJD27 amphetamine, nordazepam, oxazepam
D7TTWV ANFETAMINAS + BENZODIACEPINAS
DONO2Y amfetamine
ERWOWW Amphetamine and Nordazepam
FNSUKR Temazepam, nordiazepam
ILS9UP Amphetamine
J2FAYZ amphetamine, benzodizepines
J6WWIX Amphetamine, benzos
JLCQ3D DESMETHYLDIAZEPAM
JWS3VW diazepam, nordazepam, temazepam, amphetamine
JZ6JTH THC
KHMLHA Temazepam, Amphetamine.
KKPG9K Amfetamine, Nordazepam and Temazepam.
LFLTTZ Amphetamine groups, benzodiazepines
M8E6T6 nordazepam, temazepam, amphetamines
MDKW7J codeine, morphine and 6acetylmorphine
NDS3R3 amphetamine, temazepam, nordazepam, oxazepam
OOOQSO amphetamine, diazepam, nordazepam, oxazepam, temazepam
PSXQYE Amfetamine, Nordazepam, Oxazepam, Temazepam
Sample 2 Analysis
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Code Identified SubstancesQHGM5Q Amfetamine, Nordazepam, Oxazepam, Temazepam
RFGGQG oxazepam, nordiazepam, temazepam and amphetamine
SAV6NX Amfetamine, Nordazepam, oxazepam and Temazepam
TTAVXV Amphetamine, oxazepam, nordiazepam and temazepam.
UM0M1S Nordazepam, Amphetamine
VHDEKU Amphetamine+nordazepam+Temazepam
WIYO85 AMPHETAMINE & BENZO
WWSTWW amphetamine, nordazepam, oxazepam and temazepam
XA9AAA Amfetamine - Nordazepam
XDXVS9 Nordazepam and Temazepam
XJZEEE Amphetamine, Temazepam, Nordiazepam
XLEICI Nordazepam
XTLXKX Amphetamine, Nordazepam, Oxazepam, Temazepam
XXHQNV Nordazepamand Oxazepam
YCEET4 amfetamine
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Code Amfetamine Nordazepam Oxazepam Temazepam0709WO + + + +
-
1C2WN2 + + + +amphetamineoxazepamnordiazepamtemazepamcaffeine
1M39MI + + + +sample positive for benzodiazepines and amphetamine
2I1J7W + + + +
3ESBDT - + - -Nordazepam was detected.
5A0LTW + + + +
5D8EIT - + + +The sample contain 2105.43 ng/mL of nordazepam. Oxazepam, temazepam, theobromine and caffeine was detected.
6H33U3 ANP + ANP +Nordazepam and Temazepam was detected in sample BS2 but quantification analysis was not performed.
6ZZZCD - + ANP -2013/1/BS-2 urine contain methamphetamine(traces),nordazepam(diazepam, methamphetamine user)
76JL37 + + + +Findings indicate that the person consumed amfetamine and one of or the combination of 1,4 - benzodiazepines.
7HEM7O - + - -
98KDM8 + + - -The urine was found to contain nordiazepam and amphetamine.
ABBR1D + + + +
AKQ21O + + + +The presence of amphetamine indicates the use of amphetamine. The presence of nordiazepam, temazepam and oxazepam indicates the use of diazepam.
ANXWGY ANP + ANP +Nordazepam and temazepam detected
Statement of findings for Test Sample 2
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Code Amfetamine Nordazepam Oxazepam TemazepamBBRB0E + ANP ANP ANP
we found amphetamine in BS-2 as a controlled substance
BJG4OO - + ANP +There was temazepam consumption
BPL9ZY - + + +We also found caffeine and demoxepam in BS-2.
BVABMB + + + +sample positive to amphetamine:1197.2 (cut-off 250 ng/ml)sample positive to oxazepam:213.6 (cut-off 100 ng/ml)sample positive to nordiazepam :805.3 ng/ml (cut-off 100 ng/ml)
sample positive to temazepam: no quantification performed due to lack of certified standardCUH0QQ + + + -
IN THE ROUND AS LABELED "ROUND ICE 2013/1 SAMPLE BS-2" DETECTED DIAZEPAM, OXAZEPAM, NORDIAZEPAM AND AMPHETAMINE.
CUUFFQ + + + +
CWOOM9 + + - -
CZJD27 + + + ANPTest sample contains amphetamine (1066.26 ng/mL) using GCMS and nordazepam(1652.28 ng/mL) and oxazepam (1035.11ng/mL) using LCMSMS
D7TTWV ANP ANP ANP ANPPOSITIVO (PRESUNTIVO)
DONO2Y + + + +Amfetamine was detected in urine. Results indicate the previous consumption of controlled narcotic (illegal), amfetamine or metamfetamine.The concentration of GHB reflect the physiological concentrations.
ERWOWW + + - -Amphetamine and Nordazepam were identified by GC/MS
FNSUKR ANP + - +La coincidencia entre los tiempos de retención y fragmentogramas de un material de referencia analizado en las mismas condiciones de una muestra biológica indica la presencia de temazepam y nordiazepam en esta.
FZZZZQ - ANP ANP ANP
ILS9UP + + + +Amphetamine, Nordazepam, Temazepam, Oxazepam
J2FAYZ + + + +we have not received standards
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Code Amfetamine Nordazepam Oxazepam TemazepamJ6WWIX + + + +
Presence of metabolites
JBUJJA - - - ANP
JLCQ3D - + - -The sample BS-2 contain DESMETHYLDIAZEPAM
JWS3VW + + - +
JZ6JTH - ANP ANP ANP
KHMLHA + ANP - +In the urine sample analyzed, was detected and confirmed the presence of Temazepam, Amphetamine.
KKPG9K + + - +The sample contains amfetamine, nordazepam and temazepam.
LFLTTZ + - - -Amphetamin
M8E6T6 ANP + - +use of benzodiazepines and amphetamines
MDKW7J + + + +
NDS3R3 + + + +consumption of amphetamine and diazepam or medazepam
OOOQSO + + + +
PSXQYE + + + +Sample is positive for Amfetamine, Nordazepam, Oxazepam and Temazepam
QHGM5Q + + + +
RFGGQG + + + +sample positive for benzodiazepines and amphetamine
SAV6NX + + + +Amfetamine is used for recreationnal purposes. It's a controlled narcotic in France. Nordazepam, oxazepam and temazepam are benzodiazepines, used for their anxiolytic and sedativ properties and also to treat epilepsy. Are also 3 metabolites of diazepam and their presence may indicates a diazepam consumption.
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Code Amfetamine Nordazepam Oxazepam TemazepamTTAVXV + + + +
The oxazepam, nordiazepam and temazepam detection indicates most likely: diazepamconsumption, nevertheless the presence of these substances could come from the intake ofthe drugs itself or come from the intake of any other benzodiazepine that producesdiazepam as a metabolite.The amphetamine detection indicates the intake of the drug itself or could come from themetabolism of other drugs such as methamphetamine.
UM0M1S + + - -Screening by immunoassay technich : PositiveConformation by GC-MS : Positive
VHDEKU + + - +Sample containing Amphetamine+Nordazepam+Temazepam, substances under control
WIYO85 + + + +BS2 positive for:amphetamine, nordazepam, oxazepam & temazepam
WWSTWW + + + +
XA9AAA + + - -Amfetamine was detected, no other drugs as methamfetamine or drugs metabolizing to amfetamine were identified, this indicate that amfetamine was consumedIn the sample was detected Nordazepam, this finding indicate that Nordazepam was consumed
XDXVS9 - + - +On analysis, the sample of 2013/1/BS-2 found to contain Nordazepam and Temazepam.
XJZEEE ANP ANP ANP ANP
XLEICI - + - -Nordazepam was found in BS-2. Quantitation was not performed.
XTLXKX + + + +1. "Speed" (amphetamine) abuse. 2. Consumption of nordazepam and temazepam is most likely. However, use of other benzodiazepine type drug (e.g. diazepam) can not be excluded.
XXHQNV ANP + + -Nordazepam and Oxazepam are common metabolites of 1-4, Benzodiazepines groups of drugs
YCEET4 + ANP ANP ANP
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Identification methods for Test Sample 2
Code 110 120 150 161 170 171 180 230 231 210
0709WO SI
1C2WN2 S I I
1M39MI S SI
2I1J7W S I I
3ESBDT S I I
5A0LTW S I I
5D8EIT S I
6H33U3 I
6ZZZCD S I
76JL37 S I I
7HEM7O S I I
98KDM8 S I
ABBR1D S I I
AKQ21O S I SI I
ANXWGY S I
BBRB0E S I
BJG4OO S I
BPL9ZY S SI SI
BVABMB SI
CUH0QQ S I
CUUFFQ S I
CWOOM9 S I I
CZJD27 I I S DT kits
D7TTWV S
DONO2Y S I I
ERWOWW
S I
FNSUKR S I
FZZZZQ SI
ILS9UP S I I S Kinetic Interactions of microparticles
J2FAYZ I I immunochem
J6WWIX S I I
JBUJJA S I I I
JLCQ3D SI I
Analytical Technique Codes
Legend: S ... used for Screening I ... used for Identification SI ... used for both
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Code 110 120 150 161 170 171 180 230 231 210
JWS3VW S I
JZ6JTH S I I
KHMLHA S I
KKPG9K SI S Multi-Drug Screen Test Panel
LFLTTZ I S Dipromed Drulab screen
M8E6T6 S I
MDKW7J SI I
NDS3R3 S S I
OOOQSO S I
PSXQYE S S I I
QHGM5Q I I SI I
RFGGQG S SI
SAV6NX S I I
TTAVXV S I I I I
UM0M1S S I
VHDEKU SI I
WIYO85 S I
WWSTWW
S I I
XA9AAA S I S Lateral Flow Immunoassay
XDXVS9 I SI
XLEICI S I
XTLXKX S I I
XXHQNV S I
YCEET4 I
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Section 110 120
Response Summary Participants: 54
Frequency of use of screening and identification techniques:
H
Summary for test sample 2The response summary of all 54 participating laboratories for the identification of BS-2, frequency of use of screening andidentification techniques and false positives and negatives:
Identification of test sample 2:
False positives for Test Sample 2
2013/1-BS Copyright (c) 2013 UNODC(14)
Response SummaryParticipants: 58
Amfetamine: 39
Nordazepam: 49
Oxazepam: 30
Temazepam: 37
False negatives for BS-2 (techinque(s) used or ANP) . Within BS-2, there were: 19 false negatives for Amfetamine 9 false negatives for Nordazepam 28 false negatives for Oxazepam 21 false negatives for Temazepam
Code
Name TechniqueMDKW75 Codeine, Morphine, 6-MAM in report
JWS3VW Diazepam 180
Name6ZZZCD Metamfetamine 150
BBRB0E Diazepam 150
BPL9ZY Diazepam, Demoxazepam 231
CUH0QQ Diazepam 180
DONO2Y gamma-Hydroxybutyric acid (GHB) 180
JZ6JTH 180
Section 110 120 150 161 170 171 180 230 231 210
Screening 36 8 4 0 0 0 9 1 1 4
62.1% 13.8% 6.9% 0.0% 0.0% 0.0% 15.5% 1.7% 1.7% 6.9%
Identification 4 0 3 11 2 2 49 5 12 2
6.9% 0.0% 5.2% 19.0% 3.4% 3.4% 84.5% 8.6% 20.7% 3.4%
# labs using this technique
36 8 7 11 2 2 49 6 12 662.1% 13.8% 12.1% 19.0% 3.4% 3.4% 84.5% 10.3% 20.7% 10.3%
Response Summary Participants: 58
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Z-Scores for Test Sample 2
CODEAmfetamineMAD: 138.0
NordazepamMAD: 241.9
OxazepamMAD: 48.0
Conc. * AbsDev ZScore Conc. * AbsDev ZScore Conc. * AbsDev ZScore
0709WO 1130.0 11.5 0.1 1500.0 152.1 0.4 350.0 120.0 1.7
5A0LTW 1740.0 621.5 3.0 1652.0 0.1 0.0 410.0 60.0 0.8
5D8EIT 2105.4 453.3 1.3
76JL37 1271.0 152.5 0.7 1888.0 235.9 0.7
ABBR1D 1320.0 201.5 1.0 1830.0 177.9 0.5 450.0 20.0 0.3
BJG4OO 33383.0 31730.9 88.6
BPL9ZY 1253.3 398.8 1.1 370.1 99.9 1.4
BVABMB 1197.2 78.7 0.4 805.3 846.8 2.4 213.6 256.4 3.6
CWOOM9 950.0 168.5 0.8 1240.0 412.1 1.2
CZJD27 1066.3 52.2 0.3 1652.3 0.1 0.0 1035.1 565.1 7.9
DONO2Y 1205.0 86.5 0.4 2146.0 493.9 1.4 518.0 48.0 0.7
ILS9UP 1106.0 12.5 0.1
J2FAYZ 242.0 876.5 4.3 765.0 887.1 2.5 113.0 357.0 5.0
J6WWIX 1107.0 11.5 0.1 612.0 1040.1 2.9 645.0 175.0 2.5
KKPG9K 890.0 228.5 1.1
LFLTTZ 1642.0 523.5 2.6
M8E6T6 1900.0 247.9 0.7
MDKW7J 1100.0 18.5 0.1 1600.0 52.1 0.1 420.0 50.0 0.7
PSXQYE 806.0 312.5 1.5 3500.0 1847.9 5.2 470.0 0.0 0.0
QHGM5Q 995.0 123.5 0.6 1350.0 302.1 0.8 460.0 10.0 0.1
SAV6NX 1101.0 17.5 0.1 2004.0 351.9 1.0 497.2 27.2 0.4
WIYO85 960.0 158.5 0.8 1570.0 82.1 0.2 481.0 11.0 0.2
WWSTWW 1231.0 112.5 0.6 1580.0 72.1 0.2 550.0 80.0 1.1
XJZEEE 1606.0 487.5 2.4 1888.0 235.9 0.7
XTLXKX 1027.0 91.5 0.4 1523.0 129.1 0.4 450.0 20.0 0.3
*) Concentration: ng/ml
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
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CODETemazepamMAD: 112.0
Conc. * AbsDev ZScore
0709WO 420.0 80.0 0.5
5A0LTW 1050.0 550.0 3.3
5D8EIT
76JL37
ABBR1D 460.0 40.0 0.2
BJG4OO 17160.0 16660.0 100.4
BPL9ZY 363.0 137.0 0.8
BVABMB
CWOOM9
CZJD27
DONO2Y
ILS9UP
J2FAYZ 138.0 362.0 2.2
J6WWIX 233.0 267.0 1.6
KKPG9K 360.0 140.0 0.8
LFLTTZ
M8E6T6
MDKW7J 460.0 40.0 0.2
PSXQYE 1900.0 1400.0 8.4
QHGM5Q 480.0 20.0 0.1
SAV6NX 612.0 112.0 0.7
WIYO85 517.0 17.0 0.1
WWSTWW 500.0 0.0 0.0
XJZEEE 961.0 461.0 2.8
XTLXKX 447.0 53.0 0.3
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(28)
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Substance: BS-2/Amfetamine 138.MAD =Median = 1118.5
Round: 2013/1
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
Z-Score Report per Sample per Substance
UNODC value = 1143
(29)
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Substance: BS-2/Nordazepam 241.9MAD =Median = 1652.1
Round: 2013/1
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
Z-Score Report per Sample per Substance
UNODC value = 1727
(30)
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Substance: BS-2/Oxazepam 48MAD =Median = 470
Round: 2013/1
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
Z-Score Report per Sample per Substance
UNODC value = 457
(31)
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Substance: BS-2/Temazepam 112MAD =Median = 500
Round: 2013/1
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
Z-Score Report per Sample per Substance
UNODC value = 464
(32)
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Identified substances for Test Sample 3
Code Identified Substances0709WO GHB
2I1J7W GHB
3ESBDT None
5A0LTW GHB
76JL37 GHB
ABBR1D GHB
AKQ21O GHB
BPL9ZY GHB, GBL
CUH0QQ NONE
DONO2Y GHB
J2FAYZ nordiazepam below cut-off
JLCQ3D ANY DRUGS
JZ6JTH Morphine
MDKW7J amphetamine, nodiazepam, oxazepam and temazepam
PSXQYE gamma-Hydroxybutyric acid (GHB)
SAV6NX GHB
WWSTWW GHB
XA9AAA GHB
XTLXKX gamma-Hydroxybutyric acid
Sample 3 Analysis
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Code gamma-Hydroxybutyric acid (GHB)
0709WO +-
1C2WN2 ANPcaffeine
1M39MI ANP
2I1J7W +
3ESBDT ANPNegative
5A0LTW +
5D8EIT ANPThe contain caffeine.
6H33U3 ANPNo drugs detected in sample BS3.
6ZZZCD ANP
76JL37 +Findings indicate that the person consumed GHB.
7HEM7O -
98KDM8 ANP
ABBR1D +
AKQ21O +The presence of GHB indicates the use of GHB or GBL.
ANXWGY ANPNo drugs was detected
BBRB0E ANPwe did not find any controlled drug in BS-3
Statement of findings for Test Sample 3
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Code gamma-Hydroxybutyric acid (GHB)
BJG4OO ANPnot detected any of the substances investigated
BPL9ZY +We also found caffeine in BS-3.
BVABMB ANP
CUH0QQ -IN THE ROUND AS LABELED "ROUND ICE 2013/1 SAMPLE BS-3" NOT DETECTED METABOLITES OF COCAINE, CANNABINOIDS, BENZODIAZEPINES, AMPHETAMINES, PHENOTHIAZINES AND TRICYCLIC ANTIDEPRESSANTS.
CUUFFQ ANP
CWOOM9 -
CZJD27 ANPNo drugs found based on test performed
D7TTWV ANPNO SE DETECTÓ
DONO2Y +The concentration of GHB (>10 mg/L) reflect a suspicion case of recreative use or drug-facilitated crime.
ERWOWW -
FNSUKR ANPEn inmunoensayo una concentración menor al punto de corte indica que el sistema analítico no detectó la presencia de cocaína, cannabinoides , anfetaminas, barbitúricos ni benzodiacepinas.
FZZZZQ ANP
ILS9UP ANP
J2FAYZ -we have not received standards
J6WWIX ANP
JBUJJA ANP
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Code gamma-Hydroxybutyric acid (GHB)
JLCQ3D ANPThe sample BS-3 no contain any drugs
JWS3VW ANP
JZ6JTH ANP
KHMLHA ANP
KKPG9K ANP
LFLTTZ ANP
M8E6T6 ANP
MDKW7J ANP
NDS3R3 ANP
OOOQSO ANP
PSXQYE +Sample is positive for Gamma-Hydroxybutyric acid (GHB)
QHGM5Q -
RFGGQG ANP
SAV6NX +GHB is a controlled substance since 1999 in France. GHB has been used in a medical setting as a general anesthesic. GHB is a central nervous system used as an intoxicant
TTAVXV ANP
UM0M1S -
VHDEKU ANP
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Code gamma-Hydroxybutyric acid (GHB)
WIYO85 -BS3 was negative for supplied standards
WWSTWW +
XA9AAA +GHB was detected, cuantitication was not performed, but the sample concentration was greater than a urine sample containing 10000 ng/ml
XDXVS9 ANPOn analysis, no drugs found in the sample of 2013/1/BS-3.
XJZEEE ANP
XLEICI ANP
XTLXKX +Abuse of GHB salt or GBL or 1,4-butanediol, if there is no succinic semialdehyde dehydrogenase deficiency (SSADH).
XXHQNV ANPNone Detected
YCEET4 ANP
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Identification methods for Test Sample 3
Code 110 120 150 161 170 171 180 230 231 210
0709WO SI
1C2WN2 S I I
1M39MI S SI
2I1J7W S I I
3ESBDT S I I
5A0LTW S I I
5D8EIT S I
6H33U3 I
6ZZZCD S I
76JL37 S I I
7HEM7O S I I
98KDM8 S I
ABBR1D S I I
AKQ21O S I SI I
ANXWGY S I
BBRB0E S I
BJG4OO S I
BPL9ZY S SI SI
BVABMB SI
CUH0QQ S I
CUUFFQ S I
CWOOM9 S I I
CZJD27 I I S DT kits
D7TTWV S
DONO2Y S I I
ERWOWW
S I
FNSUKR S I
FZZZZQ SI
ILS9UP S I I S Kinetic Interactions of microparticles
J2FAYZ I I immunochem
J6WWIX S I I
JBUJJA S I I I
JLCQ3D SI I
Analytical Technique Codes
Legend: S ... used for Screening I ... used for Identification SI ... used for both
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Code 110 120 150 161 170 171 180 230 231 210
JWS3VW S I
JZ6JTH S I I
KHMLHA S I
KKPG9K SI S Multi-Drug Screen Test Panel
LFLTTZ I S Dipromed Drulab screen
M8E6T6 S I
MDKW7J SI I
NDS3R3 S S I
OOOQSO S I
PSXQYE S S I I
QHGM5Q I I SI I
RFGGQG S SI
SAV6NX S I I
TTAVXV S I I I I
UM0M1S S I
VHDEKU SI I
WIYO85 S I
WWSTWW
S I I
XA9AAA S I S Lateral Flow Immunoassay
XDXVS9 I SI
XLEICI S I
XTLXKX S I I
XXHQNV S I
YCEET4 I
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INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
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2013/1-BS Copyright (c) 2013 UNODC(40)
INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
Section
Response Summary Participants: 54
R
Frequency of use of screening and identification techniques:
Summary for The response summary of all 54 participating laboratories for the identification of BS-3, frequency of use of screening andidentification techniques and false positives and negatives:
Identification of test sample 3:
False positives for Test Sample
2013/1-BS Copyright (c) 2013 UNODC(14)
Response SummaryParticipants: 58
gamma-Hydroxybutyric acid (GHB): 13
False negatives for BS-3 . Within BS-3, there were: 8 false negatives for GHB by participants using analytical techniques
CUHOQQ (180), QHGM5Q (171/180), UM0MIS (180), WIYO85 (231), ERWOWW (180) CWOOM9 (180), 7HEM7O (171/180), J2FAYZ (180) 37 false negatives by particpants who did not carry out analyses for GHB
Code
Name TechniqueMDKW75 Amfetamine, Nordazepam, in report
MDKW75 Oxazepam, Temazepam in report
NameJZ6JTH Morphine 230
J2FAYZ Nordazepam 180
Section 110 120 150 161 170 171 180 230 231 210
Screening 36 8 4 0 0 0 9 1 1 4
62.1% 13.8% 6.9% 0.0% 0.0% 0.0% 15.5% 1.7% 1.7% 6.9%
Identification 4 0 3 11 2 2 49 5 12 2
6.9% 0.0% 5.2% 19.0% 3.4% 3.4% 84.5% 8.6% 20.7% 3.4%
# labs using this technique
36 8 7 11 2 2 49 6 12 662.1% 13.8% 12.1% 19.0% 3.4% 3.4% 84.5% 10.3% 20.7% 10.3%
Response Summary Participants: 58
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Z-Scores for Test Sample 3
CODE
gamma-Hydroxybutyric acid (GHB)MAD: 1114.5
Conc. * AbsDev ZScore
0709WO 15500.0 575.0 0.3
5A0LTW 14350.0 575.0 0.3
ABBR1D 16500.0 1575.0 1.0
BPL9ZY 14300.0 625.0 0.4
DONO2Y 17.8 14907.2 9.0
PSXQYE 140100.0 125175.075.8
SAV6NX 14283.0 642.0 0.4
WWSTWW 15579.0 654.0 0.4
XTLXKX 10543.0 4382.0 2.7
*) Concentration: ng/ml
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
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Substance: BS-3/gamma-Hydroxybutyric acid (GHB) 1114.5MAD =Median = 14925
Round: 2013/1
Z-Score has been calculated as modified Z-Score as it is a robust method and therefore relatively insensitive to outliers.Modified Z-Score = 0.6745 * AbsDev/MADAbsDev = Absolute value of the difference between a laboratory value and the median of all the laboratory values for the given drug;MAD = median absolute deviation about the median.Modified Z-scores with an absolute value of greater than 3.5 should be considered as potential outliers.Reference:Iglewicz, B. and Hoaglin, D. C.: 1993 How to Detect and Handle Outliers, American Society for Quality Control, Milwaukee, WI
Z-Score Report per Sample per Substance
UNODC value = 14427
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Identified substances for Test Sample 4
Sample 4 Analysis
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INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
Code
Response Summary Participants: 58
Identified Substances76JL37 ephedrine
1M39MI ephedrine
BPL9ZY ephedrine
KKPG9K ephedrine
MDKW7J ephedrine
NDS3R3 ephedrine
WWSTWW ephedrine
OOOQSO ephedrine
RFGGQG ephedrine
UM0M1S ephedrine
5D8EIT ephedrine
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Code [blank sample]0709WO -
1C2WN2 caffeine
1M39MI sample positive for ephedrine
2I1J7W
3ESBDT Negative
5A0LTW
5D8EIT The sample contain pseudoephedrine / ephedrine and caffeine.
6H33U3 No drugs detected in sample BS4.
6ZZZCD
76JL37 Findings indicate that the person consumed ephedrine or pseudoephedrine.
7HEM7O
98KDM8
ABBR1D
AKQ21O No drugs found.
ANXWGY No drugs was detected
BBRB0E we did not find any controlled drug in BS-4
BJG4OO not detected any of the substances investigated
Statement of findings for Test Sample 4
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INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
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Code [blank sample]BPL9ZY We also found caffeine and ephedrine in BS-4.
BVABMB
CUH0QQ IN THE ROUND AS LABELED "ROUND ICE 2013/1 SAMPLE BS-4" NOT DETECTED METABOLITES OF COCAINE, CANNABINOIDS, BENZODIAZEPINES, AMPHETAMINES, PHENOTHIAZINES AND TRICYCLIC ANTIDEPRESSANTS.
CUUFFQ
CWOOM9
CZJD27 No drugs found based on test performed
D7TTWV NO SE DETECTÓ
DONO2Y The concentration of GHB reflect the physiological concentrations.
ERWOWW
FNSUKR En inmunoensayo una concentración menor al punto de corte indica que el sistema analítico no detectó la presencia de cocaína, cannabinoides , anfetaminas, barbitúricos ni benzodiacepinas.
FZZZZQ
ILS9UP
J2FAYZ we have not received standards
J6WWIX
JBUJJA
JLCQ3D The sample BS-4 no contain any drugs
JWS3VW
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INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
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Code [blank sample]JZ6JTH
KHMLHA
KKPG9K The sample contains ephedrine.
LFLTTZ
M8E6T6
MDKW7J sample 5 contains ephedrine (GC-MS)
NDS3R3 ephedrine was found in the sample
OOOQSO ephedrine was identified in sample 2013/1/BS-4 by LC/MS and its presence was confirmed by LC/MS/MS
PSXQYE Sample contains no substance from the ICE menu
QHGM5Q
RFGGQG sample positive for ephedrine
SAV6NX
TTAVXV
UM0M1S Screening and conformation by GC-MS : Positive
VHDEKU
WIYO85 BS4 was negative for supplied standards
WWSTWW Ephedrine is found in BS-4, but ephedrine is not the controlled drug.
2013/1-BS Copyright (c) 2013 UNODC(46)
INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
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Code [blank sample]XA9AAA
XDXVS9 On analysis, no drugs found in the sample of 2013/1/BS-4.
XJZEEE
XLEICI Phenmetrazine was found in BS-4.
XTLXKX
XXHQNV None Detected
YCEET4
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INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
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Identification methods for Test Sample 4
Code 110 120 150 161 170 171 180 230 231 210
0709WO SI
1C2WN2 S I I
1M39MI S SI
2I1J7W S I I
3ESBDT S I I
5A0LTW S I I
5D8EIT S I
6H33U3 I
6ZZZCD S I
76JL37 S I I
7HEM7O S I I
98KDM8 S I
ABBR1D S I I
AKQ21O S I SI I
ANXWGY S I
BBRB0E S I
BJG4OO S I
BPL9ZY S SI SI
BVABMB SI
CUH0QQ S I
CUUFFQ S I
CWOOM9 S I I
CZJD27 I I S DT kits
D7TTWV S
DONO2Y S I I
ERWOWW
S I
FNSUKR S I
FZZZZQ SI
ILS9UP S I I S Kinetic Interactions of microparticles
J2FAYZ I I immunochem
J6WWIX S I I
JBUJJA S I I I
JLCQ3D SI I
Analytical Technique Codes
Legend: S ... used for Screening I ... used for Identification SI ... used for both
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INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
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Code 110 120 150 161 170 171 180 230 231 210
JWS3VW S I
JZ6JTH S I I
KHMLHA S I
KKPG9K SI S Multi-Drug Screen Test Panel
LFLTTZ I S Dipromed Drulab screen
M8E6T6 S I
MDKW7J SI I
NDS3R3 S S I
OOOQSO S I
PSXQYE S S I I
QHGM5Q I I SI I
RFGGQG S SI
SAV6NX S I I
TTAVXV S I I I I
UM0M1S S I
VHDEKU SI I
WIYO85 S I
WWSTWW
S I I
XA9AAA S I S Lateral Flow Immunoassay
XDXVS9 I SI
XLEICI S I
XTLXKX S I I
XXHQNV S I
YCEET4 I
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2013/1-BS Copyright (c) 2013 UNODC
INTERNATIONAL QUALITY ASSURANCE PROGRAMME (IQAP)INTERNATIONAL COLLABORATIVE EXERCISES (ICE)
False positives for Test Sample 4Code
R
Frequency of use of screening and identification techniques:
Summary for The response summary of all 54 participating laboratories for the identification of BS-4, frequency of use of screening andidentification techniques and false positives and negatives:
2013/1-BS Copyright (c) 2013 UNODC(14)
Response SummaryParticipants: 54
Identified substances for Test Sample 4
Code
CodeCode
Name TechniqueJ2FAYZ Nordazepam 180
Name
Name
DONO2Y Gamma-Hydroxybutyric acid (GHB) 180
JZ6JTH Methylecgonine, Benzoylecgonine 230
XLEICI Phenmetrazine 180
YCEET4 Metamfetamine 180
Section 110 120 150 161 170 171 180 230 231 210
Screening 36 8 4 0 0 0 9 1 1 4
62.1% 13.8% 6.9% 0.0% 0.0% 0.0% 15.5% 1.7% 1.7% 6.9%
Identification 4 0 3 11 2 2 49 5 12 2
6.9% 0.0% 5.2% 19.0% 3.4% 3.4% 84.5% 8.6% 20.7% 3.4%
# labs using this technique
36 8 7 11 2 2 49 6 12 662.1% 13.8% 12.1% 19.0% 3.4% 3.4% 84.5% 10.3% 20.7% 10.3%
Response Summary Participants: 58
Identified Substances76JL37 ephedrine
1M39MI ephedrine
BPL9ZY ephedrine
KKPG9K ephedrine
MDKW7J ephedrine
NDS3R3 ephedrine
WWSTWW ephedrine
OOOQSO ephedrine
RFGGQG ephedrine
UM0M1S ephedrine
5D8EIT ephedrine
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The Laboratory and Scientific SectionUNODC, International CentreWagramer Strasse 5, P.O. Box 500A-1400, Vienna, AUSTRIAEmail: [email protected]
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