2018INTERNATIONAL

POULTRY SCIENTIFIC

FORUMJANUARY 29 – 30

ABSTRACTS2018 International Poultry Scientific Forum

Georgia World Congress Center, Atlanta, GeorgiaJanuary 29-30, 2018

Table of Contents

SYMPOSIA AND ORAL SESSIONSMonday, January 29, 2018Milton Y Dendy Keynote Address ....................................................................................................................................................... 1Physiology, Endocrinology & Reproduction ....................................................................................................................................... 2Processing & Products ......................................................................................................................................................................... 4Metabolism & Nutrition - Enzymes ..................................................................................................................................................... 6Pathology ............................................................................................................................................................................................. 9SCAD ................................................................................................................................................................................................ 12Metabolism & Nutrition - Amino Acids ............................................................................................................................................ 15Metabolism & Nutrition - Vitamins & Minerals ................................................................................................................................ 18Metabolism & Nutrition - Feed Additives & General Nutrition ........................................................................................................ 22Environment, Management & Animal Well-Being - Behavior & Incubation ................................................................................... 29Environment, Management & Animal Well-Being - Stress Responses ............................................................................................. 32Physiology.......................................................................................................................................................................................... 35Processing and Products .................................................................................................................................................................... 36

Tuesday, January 30, 2018Environment, Management & Animal Well-Being - Stress Responses ............................................................................................. 37Environment, Management & Animal Well-Being - Behavior & Incubation ................................................................................... 38SCAD ................................................................................................................................................................................................. 40Metabolism & Nutrition - Amino Acids ............................................................................................................................................ 42Metabolism & Nutrition - Enzymes ................................................................................................................................................... 43Metabolism & Nutrition - Feed Additives ......................................................................................................................................... 47

POSTER PRESENTATIONS ............................................................................................................................................... 55Author Index ...................................................................................................................................................................................... 99

82 ABSTRACTS OF PAPERS

meat yield of broilers. Dietary treatments included: T1, control (CON), T2, 5 mg/bird/day of PA (LowPA), T3, 10 mg/bird/day of PA (MidPA), and T4, 15 mg/bird/day of PA (HighPA). All birds were weighed on d 14, 28, 42 and 49 to obtain average pen weights and feed conversion ratios. On day 49, eight birds per pen were processed and carcass and breast yield were determined. Breast filets were evaluated for the presence and sever-ity of woody breast and white striping. Differences (P < 0.05) in live bird weights between the control birds (1.659kg) and all PA treatments (pooled mean: 1.731kg) began at 28 days; however, only the LowPA carried that effect (P = 0.05) through the conclusion of the trial (3.553 vs 3.818 kg). Overall, LowPA (1.649) and MidPA (1.694) had lower (P < 0.05) FCR than the CON treatment (1.741). Increased growth observed in live bird weights in the LowPA translated to increased (P < 0.05) overall carcass weights (2.783 vs 2.991 kg) and specifically breast filet weights (0.693 vs 0.769 kg). Yields did not differ (P > 0.05) but with the increased weight feeding LowPA resulted in more total breast meat. None of the doses of PA affected (P > 0.05) woody breast (pooled mean: 1.23) or white stripping scores (pooled mean: 1.06). In conclusion utilizing dietary PA increased live bird weights, improved FCR, and increased breast filet weight. This improvement in BW and breast weight was obtained without increasing woody breast, or white stripping. These data indicate that dietary PA may increase production efficiency in broilers.Key Words: broiler, phosphatidicacid, growth, Breastyield

P279 Supplementation of broiler diets with All-G-Rich™: Effects on productivity, blood parameters and meat DHA content Colm Moran*1, Jason Keegan2, Doug Currie3, Anne Knox3 1Alltech SARL; 2Alltech Inc; 3Roslin Nutrition LtdThe nutritional value of chicken meat and eggs can be improved through dietary supplementation with omega-3 fatty acid (n-3 FA) rich microalgae. Aurantiochytrium limacinum (CCAP 4087/2, All-G-Rich™, Alltech Inc) is a docosahexaenoic acid (DHA) rich microalgae that can be produced heterotrophically in a sustainable manner. The purpose of this experiment was to determine the effect of dietary supplementation of broilers with A. limacinum over a 21 day period, on productivity, blood parameters and meat DHA content. Healthy day-old male Ross 308 chicks (n=2,240) were randomly allocated to one of four treatment groups. From day 0-21 all chicks were provided with a starter diet containing no algae. The experimental diets were provided ad libitum from day 21. Algae was add-ed as a percentage of the diet to provide a no algae control (T1=0%), and algae included at a level of 0.5 (T2=0.25%), 1 (T3=0.5%) and 5 (T4=1%) times the recommended intake. The study was conducted using 64 pens, providing 16 replicates for each treatment with 35 birds per pen. On days 0, 21 and 42, the birds were weighed and the amount of feed provided/removed was recorded per pen. On day 42, blood was taken from 1 bird per pen for haematological and biochemical analysis. One bird from each pen was sacrificed and breast and thigh samples were taken for DHA con-tent analysis. No differences in average weight gain, average feed intake or feed conversion ratio were observed between the control and supple-mented groups during any period of the trial. Overall, mortality was low (1.74%), and no differences between the groups in terms of mortality or blood haematology and biochemistry were observed, indicating that the algae was well tolerated. Each increase in dose corresponded to a signifi-cantly higher DHA content for both the thigh and breast tissue (thigh: 4.6, 19.0, 32.7 and 50.7 mg /100 g; breast tissue: 4.4, 17.6, 31.7 and 47.9 mg DHA /100 g tissue for T1, T2, T3 and T4 respectively, P<0.001). These results indicate that supplementation with All-G-Rich™ for 21 days under floor pen conditions can enrich chicken breast and thigh meat with DHA in proportion to the level of supplementation, without negatively impact-ing productivity.Key Words: Algae, Omega-3, DHA, Broiler

P280 Tolerance of broilers to diets supplemented with All-G-Rich™ Colm Moran*1, Jason Keegan2, Doug Currie3, Anne Knox3 1Alltech SARL; 2Alltech Inc; 3Roslin Nutrition LtdOmega-3 Fatty Acid (n-3 FA) rich microalgae can be sustainably pro-duced heterotrophically and added to chicken diets to increase the n-3 FA content of chicken meat and eggs, improving the nutritional value of these products. The purpose of this study was to investigate the tolerance of broilers to supplementation with a docosahexaenoic acid (DHA) rich microalgae (Aurantiochytrium limacinum, CCAP 4087/2, All-G-Rich™, Alltech Inc) to ensure its safe use. Healthy day-old male Ross 308 chicks (n=1,120) were randomly allocated to one of four diets which were provided ad libitum. Algae was included as a percentage of the diet to provide a no algae control (T1=0%), algae at the authorised maximum level (T2=0.5%), and algae at 5 (T3=2.5%) and 10 times (T4=5%) the maximum authorised intake. The study was conducted in a house of 32 pens, providing 8 replicates for each treatment with 35 birds per pen. Body weight and the amount of feed consumed was measured per pen on days 0, 21 and 42. Mortality was recorded daily. On day 42, blood was taken from 1 bird per pen for haematological and biochemical analysis. Two birds from each pen were sacrificed and breast and thigh samples were taken for DHA content analysis. No significant differences were observed in terms of performance between the control and treatment groups. The results indicated that supplementation with algae was well tolerated by all treatment groups. Mortality was less than 5% and no significant dif-ferences in mortality were observed between the 4 groups (P = 0.15). In addition, no significant differences were observed between the groups in terms of blood haematology and biochemistry with the exception of cholesterol which was lower in the T4 than the T1 group (2.93 vs 3.69 nmol/l, P = 0.02), and glutathione peroxidase which was higher in the T4 than the T1 group (119.96 vs 95.33 u/ml RBC, P = 0.02). Each increase in dose corresponded to a significantly higher DHA content for both the thigh and breast tissue (thigh: 12.1, 34.4, 89.0 and 139.5 mg /100 g; breast: 14.1, 42.5, 114.5 and 179.8 mg DHA /100 g tissue for T1, T2, T3 and T4 respectively, P<0.001). These results indicate that supplementation with up to 10 times the maximum recommended dose of All-G-Rich™ is well tolerated by broilers.Key Words: Algae, Omega-3, DHA, Broiler

P281 Effect of protected sodium butyrate in pullet performance Oscar Vazquez*1, Monica Puyalto2, Cinta Sol2, Juan Mallo2, Paulina Vazquez3 1Norel México S.A. De C.V.; 2Norel S.a.; 3Universidad Autonoma De GuerreroThe effect of protected sodium butyrate was evaluated in a pullet rearing trial under field conditions. A total of 110,027 one-day-old pullets (Lohm-ann lsl-lite) were alloted in four poultry yards and randomly distributed to two treatments: standard diet with antibiotic growth promoters (AGPs: Bacitracin Methylene Disalicylate and Colistin at 55 and 125 g/t of feed respectively) (Control), and the Control diet with AGPs plus protected sodium butyrate (Gustor N´RGY) at 1 kg/t of feed (PSB). Pullets were fed with starter and grower diets based on corn-soybean meal and nutri-ent levels according to Lohmann manual recommendations. Enzymes and coccidiostats were included in both treatments. All data were analyzed by GLM procedure (SAS 9). Body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR), flock uniformity (FU) and mortality were recorded at the end of the trial (15 weeks). There were no differences be-tween treatments for BWG, FI and FCR at seven weeks. However, at 15 weeks PSB group tended to increase BWG (1060 vs 1080 g; P=0.106) by 20 g. Mortality was not affected (P=0.406) by butyrate inclusion in the diet. Nevertheless, FU was higher in PSB treatment (83.5% vs 90%; P=0.049). We can conclude that the inclusion of protected sodium butyrate at the doses used in this test allowed to achieve a better flock uniformity and tended to improve growth performance, even if AGPs were used in the diet.Key Words: protected, sodium, butyrate, flock, uniformity

P282 Comparison of a feed or drinking water application of butyric acid in broilers challenged with necrotic enteritis Haci Bayir*GS1, Jundi Liu1, Greg Mathis2, Brett Lumpkins2, Justin Fowler1 1University of Georgia; 2southern poultry research inc.Butyric acid has been identified as a potential feeding strategy for manag-ing necrotic enteritis (NE) in broilers raised without antibiotics. This study was conducted to evaluate the effect of butyric acid (SmartFeedsUSA) when added in either the feed or the water on growth performance and the alleviation of NE. A basal diet was formulated and fed to a challenged and non-challenged control treatment. Two additional treatments received butyrate either in the feed or the drinking water. A total of 160 Cobb-Cobb male chicks were obtained on the day of hatch and randomly distributed among treatment pens (8 birds/pen). Birds from challenged treatments were orally gavaged with ~5000 Eimeria maxima oocysts on day 14. On day 19, 20 and 21, the challenged birds received 108 cfu/ml Clostridium perfingens. Body weight and feed intake were recorded on day 14, 21 and 28, and mortality was recorded daily. On day 21, 3 birds per pen were randomly selected and scored for the incidence and severity of intesti-nal lesions. Data were analyzed using one-way ANOVA via SPSS, with significant differences (P ≤ 0.05) separated by Duncan’s Multiple Range Tests. Results showed no significant differences in BW among treatments prior to the challenge on day 14. On day 21, all challenged treatments showed lower BW and higher FCR compared to the non-challenged con-trol. For BW gain post-challenge, the water-added butyrate was higher than the challenged control, and both forms of butyrate showed improved FCR. When butyrate was added in the water, birds ended with BW and FCR comparable to the non-challenged treatment on day 28. On both day 21 and 28, in treatments with butyrate in either the feed or water, mortality was lower than the challenged control. In this study, butyrate offered ben-efits to growth and gut health in NE-challenged broilers over the control. Further, a drinking water application showed a greater effect than when added in the feed.Key Words: butyric acid, necrotic enteritis, broilers, gut health

P283 Use of protected sodium butyrate in broilers under antibiotic free production system, a field trial Oscar Vazquez*1, David Arteaga1, Monica Puyalto2, Cinta Sol2, Juan Mallo2 1Norel México S.A. DE C.V.; 2Norel S.AThe current need to produce chickens without antibiotic growth promot-ers (AGPs) is a major challenge for producers; organic acids have been considered a viable alternative for several years. The aim of this trial was to compare the effect of protected sodium butyrate (Gustor N’RGY) and AGPs on productive parameters in broilers, under field conditions. Ten thousand one-day-old male broilers Ross were divided in two pens and randomly distributed in two treatments: Control treatment (C), a standard diet with AGP combination (Bacitracin Methylene Disalicylate, Colistin, Halquinol) and experimental treatment (PSB), the same standard diet without AGP and protected sodium butyrate (Gustor N’RGY) added at 2 kg/t. Live body weight (BW), feed intake (FI), feed conversion ratio (FCR), flock uniformity (FU) and mortality were recorded at the end of the trial. The BW was 21 g higher at the end of the trial for PSB (2.955 kg vs 2.976 kg), while fed intake was not different (5.44 kg), and as a result the FCR was 0.6% lower in PSB (1.84 vs 1.83). The FU was not different between treatments (9.25%), although the mortality was 1.2% lower in C group (5.5 vs 6.7%). Finally, the productivity index was similar between treatments (320 vs 325). It can be concluded that under the conditions of this trial the use of protected sodium butyrate in broiler diets allows to get similar productive performance than AGPs.Key Words: sodium, butyrate, antibiotic, growth, promotors