Interlaboratory study animal proteins in feed 2013 v2
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IAG ring test animal proteins 2013
L. van Raamsdonk, V. Pinckaers, J. Vliege
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Goals
● Quality support of participants
● Method performance
Ring test animal proteins
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Ring test animal proteins
Procedure
● Preparation of samples; send 5 March
● Examination; return 12 April
● Preparation of report; draft ready 6 June
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Ring test animal proteins
Materials, based on an artificial feed
● Blank sample
● Sample with fish meal 2%
● Sample with 0.05% ruminant MBM (Uruguay)
● Sample with 0.1% of tricalcium phosphate (TCP)
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Ring test animal proteins
Microscopic method
● New amended version of R (EC) 152/2009
● Effective from 12 February, but no official SOPs at the time of the ring test
● New elements in procedure
● Implemented in ring test: examination of flotate or raw material
● Not implemented: repetition of examination
● Not implemented: official report texts
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Ring test animal proteins
microscopy 53 participants; 53 sets
PCR 7 participants; 10 targets
Immunochemistry none
Response
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Ring test animal proteins
Results
● Background of materials
● Accuracy fish
● Accuracy terrestrial animals
● Accuracy flotate or raw material
● Quantification
● Application of method parameters
● Results of PCR
● Evaluation
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Ring test animal proteins
0.96
0.98
0.92
A: blank
B: 2 % fish
C: 0.05 % MBM
D: 0.1 % TCP
specificity sensitivity
0.98
Results fish
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Ring test animal proteins
0.94
0.98
specificity sensitivity
1.0
0.92 *
Results terrestrial animals
A: blank
B: 2 % fish
C: 0.05 % MBM
D: 0.1 % TCP
*: EU member states only: 0.98
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Ring test animal proteins
TCP identification
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Ring test animal proteins
0.91
specificity sensitivity
0.83
0.34
0.68
Results flotate or raw sample material (target: animal material in general)
A: blank
B: 2 % fish
C: 0.05 % MBM
D: 0.1 % TCP
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Ring test animal proteins
Method application
● Tendency to make smaller slides (small cover glass: n=38)
● Increasing use of special glass with cock
● Decreasing use of staining of sediment
● Unchanged frequency of using a binocular
● Amount of sediment used: 1 – 100 %
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Ring test animal proteins
10 gram of material
200 milligram sediment
20 mg
Examination:
20 mg
1
gr
Example: 0.05% 20 particles
20 p
Result: positive
2 p
Result: suspect
Amount of sediment used
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Ring test animal proteins
1.0
1.0
0.81
< 50% >= 50%
n=14 n=24
1.0
1.0
1.0
A: blank
B: 2 % fish
C: 0.05 % MBM
D: 0.1 % TCP *
*: p = 0.053
Amount of sediment used: sensitivity
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Ring test animal proteins
Recommendations
● Improvement of examination of flotate or raw
material
● Method: full application
● Harmonisation of the method
● Further attention to detection of TCP
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Ring test animal proteins
Thanks to:
● All participants of the study
● IAG board for cooperation
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Thank you for
your attention
Questions?