Interaction between glycoproteins and lectins studied using AFM
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0,0 0,2 0,4 0,6 0,8 1,0 0 10 20 30 40 50 60 F adh [nN ] events F= 1 91 ± 4 pN F= 29 4 ± 7 pN F= 400 ± 13 pN -200 -100 0 100 0 1 Interaction between CaY and ConA 0.20 nN 0.29 nN F o rc e [n N] P o sitio n o f sam p le [n m ] -1000 -750 -500 -250 0 250 -5 0 5 Interaction between CaY and glutaraldehyde Fo rce [n N ] 4.8 nN P o sitio n o f sam p le [n m ] Interaction between glycoproteins and lectins studied Interaction between glycoproteins and lectins studied using AFM using AFM Kateryna Lebed, Joanna Gryboś, Grażyna Pyka–Fościak, Małgorzata Lekka, Jan Styczeń The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland ▪ ▪ Protein immobilization using microcontact Protein immobilization using microcontact printing method was performed with good printing method was performed with good reproducibility reproducibility ▪ ▪ The calculated unbinding forces between The calculated unbinding forces between concanavalin A and carboxypeptidase Y was about concanavalin A and carboxypeptidase Y was about 100 pN for both immobilization ways indicating 100 pN for both immobilization ways indicating that microcontact printing technique did not that microcontact printing technique did not change biological activity of proteins change biological activity of proteins ▪ ▪ Patterned substrate with different chemical Patterned substrate with different chemical domains can be identified using force domains can be identified using force spectroscopy spectroscopy 1. 1. PROTEIN PATTERNING PROTEIN PATTERNING PDMS - polydimethylsilane PDMS - polydimethylsilane stamps stamps 6. 6. CONCLUSIONS CONCLUSIONS 0,0 0,2 0,4 0,6 0,8 1,0 0 5 10 15 20 25 30 35 F adh [nN ] ev e n ts F= 221 ± 3 pN F= 330 ± 3 pN F= 437 ± 10 pN The unbinding force for single molecular pair: The calculated binding forces between The calculated binding forces between concanavalin A and carboxypeptidase Y was concanavalin A and carboxypeptidase Y was about 100 pN about 100 pN in both cases in both cases . . CASE a: CASE a: (Con A immobilized using polymeric (Con A immobilized using polymeric stamp) stamp) F a = 105 ± 2 pN F F b b = = 108 108 ± 2 pN CASE b: CASE b: (Con A immobilized without (Con A immobilized without patterning) patterning) Adhesion force me a surements 2. 2. MOTIVATION MOTIVATION The formation of protein arrays onto solid The formation of protein arrays onto solid surfaces using microcontact printing surfaces using microcontact printing technique as it has many potential technique as it has many potential applications including the development of applications including the development of advanced biosensors. advanced biosensors. Microcontact printing method as a method for Microcontact printing method as a method for firm firm protein protein anchor anchor ing ing onto a surface without onto a surface without a a ffecting their activity. ffecting their activity. AFM images PDMS stamps PDMS stamps depth of holes 1.5 depth of holes 1.5 µ µ m m diameter of circle 5 diameter of circle 5 µ µ m m height of height of protein layer protein layer ≈ ≈ 10 nm 10 nm width of stripes width of stripes ≈ ≈ 5.1 5.1 µ µ m m diameter of circle diameter of circle ≈ ≈ 5.1 5.1 µ µ m m depth of holes 1.5 depth of holes 1.5 µ µ m m width of stripe 5 width of stripe 5 µ µ m m Con A micropatterns Con A micropatterns 5 5 . . RESULTS RESULTS Patterned substrate with different Patterned substrate with different chemical domains can be identified using chemical domains can be identified using force spectroscopy: force spectroscopy: Adhesion maps topograp hy topograp hy adhesion map adhesion map 3. 3. ATOMIC FORCE MICROSCOPY ATOMIC FORCE MICROSCOPY The studied protein–carbohydrate interaction The studied protein–carbohydrate interaction was represented by concanavalin A ( was represented by concanavalin A ( C C on A) and on A) and carboxypeptidase Y (CaY) pair. carboxypeptidase Y (CaY) pair. Measurements were performed in liquid i.e. TBS Measurements were performed in liquid i.e. TBS buffer containing 1mM concentrations of Ca buffer containing 1mM concentrations of Ca ++ ++ and Mn and Mn ++ ++ , at room temperature. , at room temperature. Carboxypeptida s e Y (CaY) Concanavalin A (ConA) 4. 4. MATERIALS MATERIALS ConA – CaY force ConA – glutaraldehyde force Force distribution The unbinding force for single molecular pair:
description
Interaction between glycoproteins and lectins studied using AFM. Kateryna Lebed, Joanna Gryboś, Grażyna Pyka–Fościak, Małgorzata Lekka, Jan Styczeń The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland. - PowerPoint PPT Presentation
Transcript of Interaction between glycoproteins and lectins studied using AFM
0,0 0,2 0,4 0,6 0,8 1,00
10
20
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F adh [nN]
even
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F= 191 ± 4 pN
F= 294 ± 7 pN
F= 400 ± 13 pN
-200 -100 0 100
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1Interaction between
CaY and ConA
0.20 nN 0.29 nN
F
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nN
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Position of sample [nm]
-1000 -750 -500 -250 0 250-5
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CaY and glutaraldehyde
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rce
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4.8 nN
Position of sample [nm]
Interaction between glycoproteins and lectins studied using AFMInteraction between glycoproteins and lectins studied using AFM
Kateryna Lebed, Joanna Gryboś, Grażyna Pyka–Fościak, Małgorzata Lekka, Jan StyczeńThe Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences,
Radzikowskiego 152, 31-342 Kraków, Poland
▪▪ Protein immobilization using microcontact printing method Protein immobilization using microcontact printing method was performed with good reproducibility was performed with good reproducibility ▪▪ The calculated unbinding forces between concanavalin A and The calculated unbinding forces between concanavalin A and carboxypeptidase Y was about 100 pN for both immobilization ways carboxypeptidase Y was about 100 pN for both immobilization ways indicating that microcontact printing technique did not change indicating that microcontact printing technique did not change biological activity of proteinsbiological activity of proteins ▪▪ Patterned substrate with different chemical domains can be Patterned substrate with different chemical domains can be identified using force spectroscopyidentified using force spectroscopy
1.1. PROTEIN PATTERNINGPROTEIN PATTERNING
PDMS - polydimethylsilane stampsPDMS - polydimethylsilane stamps
6.6. CONCLUSIONSCONCLUSIONS
0,0 0,2 0,4 0,6 0,8 1,00
5
10
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25
30
35
F adh [nN]
even
ts
F= 221 ± 3 pN
F= 330 ± 3 pN
F= 437 ± 10 pN
The unbinding force for single molecular pair:
The calculated binding forces between concanavalin A The calculated binding forces between concanavalin A and carboxypeptidase Y was about 100 pN and carboxypeptidase Y was about 100 pN in both casesin both cases. .
CASE a:CASE a: (Con A immobilized using polymeric stamp)(Con A immobilized using polymeric stamp)
Fa = 105 ± 2 pN
FFbb == 108 108 ± 2 pN
CASE b:CASE b: (Con A immobilized without patterning)(Con A immobilized without patterning)
Adhesion force measurements
2.2. MOTIVATIONMOTIVATION
The formation of protein arrays onto solid surfaces using The formation of protein arrays onto solid surfaces using microcontact printing technique as it has many potential microcontact printing technique as it has many potential applications including the development of advanced applications including the development of advanced biosensors.biosensors.
Microcontact printing method as a method for Microcontact printing method as a method for firm firm protein protein anchoranchoring ing onto a surface without onto a surface without aaffecting their activity.ffecting their activity.
AFM images PDMS stampsPDMS stamps
depth of holes 1.5 depth of holes 1.5 µµmmdiameter of circle 5 diameter of circle 5 µµmm
height ofheight of protein layer protein layer ≈≈ 10 nm 10 nm
width of stripes width of stripes ≈≈ 5.1 5.1 µµmmdiameter of circle diameter of circle ≈ ≈ 5.1 5.1 µµmm
depth of holes 1.5 depth of holes 1.5 µµmmwidth of stripe 5 width of stripe 5 µµmm
Con A micropatternsCon A micropatterns
55.. RESULTSRESULTS
Patterned substrate with different chemical domains can Patterned substrate with different chemical domains can be identified using force spectroscopy:be identified using force spectroscopy:
Adhesion maps
topography topography
adhesion mapadhesion map
3.3. ATOMIC FORCE MICROSCOPYATOMIC FORCE MICROSCOPY
The studied protein–carbohydrate interaction was represented by The studied protein–carbohydrate interaction was represented by concanavalin A (concanavalin A (CCon A) and carboxypeptidase Y (CaY) pair. on A) and carboxypeptidase Y (CaY) pair. Measurements were performed in liquid i.e. TBS buffer Measurements were performed in liquid i.e. TBS buffer containing 1mM concentrations of Cacontaining 1mM concentrations of Ca++++ and Mn and Mn++++, at room , at room temperature.temperature.
Carboxypeptidase Y (CaY)
Concanavalin A (ConA)
4.4. MATERIALSMATERIALS
ConA – CaYforce
ConA – glutaraldehydeforce
Force distribution
The unbinding force for single molecular pair:
