Institute of Microbiology Institute of Microbiology

Chinese Academy of Chinese Academy of

SciencesSciences•Microbial Resources

•Applied & Industrial Microbiology

•Pathogenic Microbiology

•Microbial Resources

•Applied & Industrial Microbiology

•Pathogenic Microbiology

Advisory Committee

International Advisory Panel

National Advisory Panel

Academic Committee

Academic Degree Committee

Development Committee

Culture Collect. Center

Microbial Analy. Center

High-throughputScreening Platform

Tech. Support Platform

Information Center

Administrative System

Center for Microbial Resources

State Key Lab of Microbial Resources

CAS Key Lab of Syst. Mycol. & Lichenol.

Center for Microbial Genome Research

Co-op with BeijingGenomics Inst., CAS

National Microbial Engineering Center

Center for Molecular Immunology

Center for Molecular Virology

Center for Agri. Biotech.Co-op with Inst. Genetics & Develop

. Biol., CAS

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Energy & Industrial Biotechnology

Developmental StrategyDevelopmental Strategy Construct Modern

Biotechnology Platforms

Human Resources

Co-op with Industry

International Co-op

Broad International Co-operationBroad International Co-operationBroad International Co-operationBroad International Co-operation

MultiGenome Access Technology

for Industrial Catalysts

(MGATech)

An EU - China Collaboration

European Commission Fifth Framework RTD ProgrammeQuality of Life and Management of Living ResourcesKey Action 3: The Cell FactoryRTD Priority 3.3.4: Identification and Sustainable Use of Metabolic and Genetic Diversity as a Source of New Valuable Products     

Review of FP5 Review of FP5 in IMCASin IMCAS

Review of FP5 Review of FP5 in IMCASin IMCAS

MultiGenome Access Technology for Industrial CatalystsAn EU – China Collaboration

Objectives• Obtain material from a range of unique Chinese hypersaline and

thermal sites• Survey microbial biodiversity, and establish and archive a

collection of novel microbes• Develop methods for the efficient isolation and recovery of

microbial genes from these sites • Identify and produce high added value new biomolecules derived

from these sites and assess these new biomolecules for commercial exploitation

Overview

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These Chinese extreme environments are of outstanding significance as microbial ecosystems with unique chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as sources of new and valuable enzymes.

Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the identification and production of valuable new enzymes for sustainable applications in biotechnological processes and consumer products. This special collaboration, which is the first microbial biotechnology collaboration between the EU and China, will provide substantial opportunities for the development of European and Chinese biotechnology.

European Commission Fifth Framework RTD Programme

Thematic Programme : Quality of Life and Management of Living Resources

Key Action 3 : The Cell Factory

RTD Priority 3.3.4 : Identification and Sustainable Use of Metabolic and Genetic Diversity as a Source of New Valuable Products

Project Number: QLK3-CT-2002-01972

Hydrothermal outbreak, upper site small poolRehai thermal area

Pearl Spring – Rehai thermal area

Sampling algal mat at large basinLangpu geothermal area

Deliverables and Achievements

• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province (Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take place in September 2003.

• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially processed on site . There has been good progress with the isolation of DNA from geothermal sites visited during the first sampling expedition and we have begun to generate bacterial gene libraries derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae and protozoans) has been constructed from a hot spring algal mat.

• Organism Acquisition: We will generate and archive culture collections of microbes from these sites. The first joint paper by Consortium members has been submitted for publishing approval.

• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic resources derived from these expeditions and technologies.

The Consortium

•Prof. Bill Grant- University of Leicester, UK

•Prof. Antonio Ventosa- University of Sevilla, Spain

•Prof. Don Cowan- University of the Western Cape, South Africa

•Prof. Ma Yanhe- Institute of Microbiology, Chinese Academy of Sciences

•Prof. Brian Jones- Genencor International BV, The Netherlands

极 端 微 生 物 实 验 室

Contacts Coordinator: Prof. Bill Grant, EU Scientific Officer: Dr. Indridi Benediktsson University of Leicester, UK Indridi.benediktsson@cec.eu.int wdg1@le.ac.uk

The Sampling Team

Hydrothermal outbreak poolRehai thermal area

Extremozyme genes

• DNA library• Metagenomic

DNA Libraries• cDNA Library

-Mannanase -Amylase -Xylanase Lipase Catalase …….

High thoughput screening

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating Water Management for the Valorisation of Agric

ultural Wastes in Paper Industry

An EU - China Collaboration

European Commission Fourth Framework RTD Programme

Thematic Programme:Basic Natural Resources

Project number:ERBIC18CT980284

     

Review of FP4 Review of FP4 in IMCASin IMCAS

Review of FP4 Review of FP4 in IMCASin IMCAS

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating W

ater Management for the Valorisation of Agricultural Wastes in Paper Industry

Activities:

To achieve these objectives, the proposed project has been constructed with an integrated multidisciplinary approach combining complementary Chinese and European industrial partners including small enterprises in close association with academics originating from both countries.

Objectives:

1.Water pollution prevention: Development of a fungal enzyme process using mainly laccases for improving annual plant kraft pulp bleachability.

2.Water pollution treatment and recycling: Design of a fungal process for effluent detoxification and decolourization in order to facilitate the subsequent biological treatment and water recycling.

European Commission Fourth Framework RTD Programme Thematic Programme : Basic Natural Resources Project Number: ERBIC18CT980284

Coordinator: Prof.Dr. Jean-Claude Sigoillot Institut National De La recherche Agronomique jcs@esil.univ-mrs.fr

The Consortium: -DR.GUIGAN FANG CHINESE ACADEMY OF FORESTRY -DR.HEINZ JELLER ANDRITZ AG, AUSTRIA -DR.LIN GUO

INSTITUTE OF MICROBIOLOGY, CAS

Ongoing International Cooperative ProjectsOngoing International Cooperative Projects

•Design new chromegens

Oxoid-Fisher Limited,UK

•Biodiversity and conservation of important bio-resources

Royal Botanic Gardens, Kew, UK

•Biodiversity of mycorrhyzal bacteria in forests of China and UK

Oxford University,UK

•Acidophilic actinomycetes as a source of new natural products

University of Newcastle, UK

IMCAS look forward to establishing the long-term cooperation with EU

An EU – China Collaboration

An EU – China Collaboration

Thank YouThank You