Innovation and Development of Study Field Nanomaterials at ... · FRAP1 0 50 100 150 200 250...
Transcript of Innovation and Development of Study Field Nanomaterials at ... · FRAP1 0 50 100 150 200 250...
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These materials have been developed within the ESF project: Innovation and development of study field Nanomaterials at the Technical University of Liberec
Innovation and Development of Study Field Nanomaterials at the Technical University of Liberec
nano.tul.cz
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Our aim is to produce the smallest diamonds
in the world!
NANODIAM = nanodiamonds
in form of layers or powders
They are so small that it's possible to
see them only by Atomic Force
Microscope.
Very thin layer consists of tiny
crystals of diamond, so tiny that one
diamond crystal is of the size of
nanometers, one millionth of a
millimeter 2
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Nanodiamond Particles
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FRAP1
0
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Control Diamond D4 Diamond D5 Diamond M1 Graphite Chromium
mR
NA
copie
sper
1000
HP
RT
1m
RN
Acopie
s
JUN
0
100
200
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400
500
600
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Control Diamond D4 Diamond D5 Diamond M1 Graphite Chromium
mR
NA
copie
sper
1000
HP
RT
1m
RN
Acopie
s
Molecular level Determination of nanodiamonds bioactivity
Gene name Encoded protein PCR product
length
Main inducing stimulus
JUN Jun activation domain binding
protein
118 Cellular stress
FRAP1 FKBP-rapamycin associated
protein 1
141 Genotoxic stress
K.Mitura, Ł.Pułaski, K.Kania, S.Mitura, G.Bartosz: „Biocompatibility assay of nanocarbon preparations based on
transcriptomic stress biomarkers”, Int. Conf. 4-th Nanodiamond and Related Materials jointly with 6-th Diamond and
Related Films, Zakopane, Abstract Book, (2005), 165. 4
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Glioblastoma multiforme Glioblastoma multiforme cells were cultured on the
chorioallantoic membrane of chicken embryo and after 7 days of incubation, were treated with carbon nanoparticles administered in ovo to the tumor. Diamond nanoparticles significantly decreased tumor mass and volume and vessel area. Quantitative real-time polymerase chain reaction analysis showed downregulated fibroblast growth factor-2 and vascular endothelial growth factor expression at the messenger ribonucleic acid level. The present results demonstrate antiangiogenic activity of carbon nanoparticles, making them potential factors for anticancer therapy.
M.Grodzik, E.Sawosz, M.Wierzbicki, P.Orłowski, A.Hotowy, T.Niemiec, M.Szmidt, K.Mitura, A.Chwalibog, International Journal of Nanomedicine, 6, (2011), 3041 – 3048.
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Microorganisms &Nanodiamonds
Salmonella E
Listeria m. Staphylococcus a.
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Salmonella E.
Listeria m. Staphylococcus a.
Nanodiamonds
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Ag
Salmonella E.
Staphylococcus a.
Listeria m.
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Au
Salmonella E.
Staphylococcus a. 9
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In ovo examination
with nanodiamond particles
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6 day of chicken embryogenesis
eye brain heart
12 day of chicken embryogenesis
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E.Sawosz, T.Niemiec, K.Mitura: Chicken
embryon 12 day – vessel system nanodiamond
particle introduced to embryon in the second
day (300ul-50 ppm C ),
NanoSMat, Rome, 2009.
vessel
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CAM of 12-day old chicken embryos implanted with a sponge loaded with; (A) PBS (control),
(B) VEGF (proangiogenic control), (C) diamond nanoparticle suspended in water at a level of 50 ppm
and (D) with aglomerate of nanodiamond placed onto CAM after injection of diamond nanoparticles
(5000 ppm) in ovo
A B
C D
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14-day old chicken embryo’s chorioallantoic membrane (CAM) after
treatment with;
(A,D) control (PBS) and diamond nanoparticles suspended in PBS at a level:
(B,E) 500 ppm, (C,F) 5000 ppm.
A B C
D E F
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Distribution of diamond nanoparticles and their aglomeration in chicken embryo’s CAM after injection of diamond
nanoparticles hydrosol (5000 ppm) in ovo; (A) diamond nanoparticles flow inside blood vessels, (B) diamond
nanoparticles flow inside blood vessels and aglomerates placed outside the vessels, (C) diamond nanoparticles
aglomerates located arounded vessels to force them, (D) diamond nanoparticles force membrane
A B
C D 16
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Nanodiamond Particles and Nanospider
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Centre for Nanomaterials, Advanced Technology & Innovations,
Technical University of Liberec, Czech
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30 kV
Capillary
collector
Electrospinning-apparatus
Centre for Nanomaterials, Advanced Technology & Innovations
Needle electrospinning:
David Lukas
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E
Water solution of poly(vinyl alcohol)
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Centre for Nanomaterials, Advanced Technology & Innovations
Elmarco - Liberec
(WO/2005/024101) A METHOD OF NANOFIBRES PRODUCTION FROM A POLYMER SOLUTION USING ELECTROSTATIC SPINNING AND A DEVICE FOR CARRYING OUT THE METHOD
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20 m
Nanofibers
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Filtration Tisuue engineering Drug delivery Membrains Soundproofed m. Sorbents „Sponge” -Hydrogen container -Ions container etc.
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X-rays from Nanospace
DPP addition
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γ - aminobutyric acid
and
N-methyl-4-aminobutyric acid
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X-rays from Nanospace
DPP addition
colle
cto
r
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Few grams of nanospider has the same surface area as of the football field….
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Ideas:
• Sponge made from nanospider and modificated
ND inclusions as a containar for hydrogen
• Sponge made from nanospider and modificated
ND inclusion as a source of ions (with very
extended surface)
• After information about application of
detonation ND as solid lubricant - additive for
engine oil – we carry out research in this area.
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Centre of Excellence NANODIAM
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CENTRE OF EXCELLENCE NANODIAM
Thank you for your attention
68thIUVSTA WORKSHOP
„Multifunctional Surface Engineering for Advanced Energy Applications"
December 9-13, 2012. City University of Hong Kong
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Results of clinical research – patch test with Nanodiamond Cream
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Clinical research with Nanodiamond Cream
Patch tests on the skin of beck
in patient with allergic diseases 1. Irritation reactions (IR)
2. Allergic reactions
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Bioactivity of diamond
The most important is the bioactivity of diamond powder in human organism.
This mechanism is based on the influence of diamond on membrane cell and DNA in nucleus.
Diamond Powder is a potential medicine because in examinations in vitro and in clinical research has antioxidant, antiinflammatory, anticancerogenic and antiallergic prpperties.
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Application of Nanodiamond Cream
Antiallergic medicine in Atopic Contact Dermatits, Contact Allergy, Hypersensitivity on metals on human skin in external application.
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Application of Nanodiamond Cream
Paramedical substance in treatment of bacterial skin diseases (staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae).
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Application of Nanodiamond Cream
Cosmetic – cream with antioxidant property, anti-age, hypoallergic, to sensitive skin, for all types of face skin, anti-wrinkle.
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After Plasma modification – possibility of depositon
effectual substance on nanofibres
PCL befor
modification
PCL after plasma modification and
deposition effectual substance on its
surface
PLASMA MODIFICATION OF NANOFIBRES
PU befor
modification
PU after plasma modification and
deposition effectual substance on its
surface
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H
H
H
H
H
H
H
H
H
+
+
H3O+
H2O
H2O
H2O
OH¯
H2
H2O
CO2
O2
N2
Diamond Layer of water Air
Ristein J., Riedel M., Mantel B. F., Stammler M., Ley L.: „Surface doping: a special
feature of diamond”; Journal of Physics: Condensed Matter; 13(2001) 8979-8987
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