Innovación en establecido concepto Agilent, extendido ... · Innovación en Analizadores: Un...

Innovación en

Analizadores: Un

establecido concepto

Agilent,

extendido ahora a todas

las necesidades de su

laboratorio

Juan Aybar

GC & GCMS

Marketing Program Manager

Agilent Technologies

Today’s Challenges Expecting More with Less

• Pressure to increase productivity

• Limited time for method development

Operating Expenses

• Resource limitations

• Reduced technical skills

Human Resources

• Meeting regulatory compliance

• Trace analysis in complex matrices Technology

2 Agilent Solutions

Solutions from the Past

La “solución” al problema tiene un significado

diferente en función del usuario y su entorno

TRATAR DE DAR RESPUESTA A TODO ELLO NO SIEMPRE ES SENCILLO

Solutions

Level 1 Level 2 Level 3 Level 4 Level 5

Instrument

installed

Checkout

Application

specific

column Application

specific

checkout

3rd party

support

Validated

method

Agilent

Support

√ √ √ √ √

√ √ √ √ √

√ √ √ √

√ √ √

√

√

√ Agilent GC and

GCMS Analyzers

Portfolio

The missing element

Application expertise

Partner Labs

The SOP of a Level 5 Solution

Agilent GC and GC/MS Solutions

Minimizes Method Development Requirements

Standard, pre-configured analyzers

Multi-residue, target list analyzers

HW only custom solutions

8 Agilent Solutions

Agilent GC, GC/MS Analyzer Concept Easy to Order, Specific to Required Application

9 Agilent Solutions

App. optimized

consumables Application

report

System with

application setup

w/o Analyzer

Analyzer trained

CE for I&F

Std. HW, SW

Configured for specific method with factory setup,

and chemically tested then field verified system

Custom

er CD

http://www.agilent.com/about/newsroom/lsca/imagelibrary/images/lsca_98.jpg

http://www.sisweb.com/art/sge/gccol.jpg

Analyzers Pre-configured to Compliance Methods Fast RGA: Configured per ASTM D1945, D1946, UOP 539

G3445B Agilent 7890 Series GC for Analyzer

• G3445B Option 1 x 112 (Capillary S/Sl inlet with EPC)

• G3445B Option 1 x 210 (FID w/EPC)

• G3445B Option 1 x 220 (TCD with EPC)

• G3445B Option 1 x 221 (TCD mounted on left side)

• G3445B Option 1 x 529 (Three Channel Fast RGA)

G3440A Option 2 x 309 (Pneumatics Control Module)

G3440A Option 702 (6-Port Valve - Column Isolation)

G3440A Option 708 ( 6-Port Valve - Sequence Reversal)

G3440A Option 754 (Four automated valves)

G3440A Option 2 x 801 (10-Port Valve - Gas Sampling)

G3440A Option 861 (Install GSV or LSV on left side of GC)

G3440A Option 701 Unheated (6-Port Valve - GSV)

G3440A Option 860 (Interface a GSV or LSV to S/Sl inlet)

G3440A Option 3 x 868 (Substitute Hastelloy-C valve - 6 port)

G3440A Option 2 x 869 (Substitute Hastelloy-C valve - 10 port)

G3440A Option 5 x 873 (Zero Volume Union to 0.32mm Column)

G3440A Option 874 (Install valve downstream)

19091P-S12 25 m x 0.32mm x 8um HP-PLOT/Al2O3 column

G1580-90200 Customer data CD

1460-1914 Capillary column hanger

19251-60540 Liner,split,straight,glasswool,non-deact

G3440-20008 Inlet filter in Hastelloy

G3440-20007 Spare Hastelloy Frits

G3440-20004 Sample Loop 100uL, Hastelloy,SL100CW-HC

G3440-20005 Sample Loop 0.5mL, Hastelloy,SL500CW-HC

G3440-20006 Sample Loop 25uL, in Nickel,SL25CW-Ni

G3591-82022 8Ft MolSieve 5A 60/80 in nickel

G3591-82020 3Ft HayeSep Q 80/100, Ni

123-1015 15m x 0.32mm x 5.00um DB-1 capillary column

G3182-60580 CPM Union, Inert

G1580-61160 NiCat HTR/Sensor

G3435-60503 FPD Electronic Cover

G3435-80500 Single FPD Detector Top Cover

5190-0519 RGA Checkout Sample with Oxygen

PC16218 1.7m HayeSep A 80/100 micro-packed column

PC16211 2Ft HayeSep A 80/100 micro-packed column

PC16219 1.5 m Mol Sieve 5A 80/100 micro-packed column

HCE1 Heated Column Enclosure

System pluming and setup according to application

Factory Application Checkout and Testing

Analyzer Manuel

CE specialist onsite I&F

Analytical Confidence: analyzer option

includes required hardware (valves with

control and method specific consumables),

analytical method, and custom report

10 Agilent Solutions

Column1, 2, 3

are wound

around the

column mandrel

Isothermal Column

Enclosure

Fast RGA -- G3445B #529 Factory Configured and Chemically Tested

optional


Fast RGA – G3445B #529 Chromatograms

Hydrocarbon Channel

Permanent and Light Gases Channel

Hydrogen Channel

7.5 min analysis


Pre-configured standard method analyzers Per Compliance Methods, e.g., ASTM, EN, UOP, GPA

Searchable, 139 pages, Agilent HPI Solution Guide PN: 5991-1561EN






Exclusive analyzer configurations



• Facilitates system-to-system method transfer and column replacement

Retention Time Locking

• Large-volume injection enhances trace-level detection Multimode Inlet (MMI)

• Improves uptime and shortens analysis times

Capillary Flow Technology (CFT) and

Backflush

• Comprehensive and optimized MRM database for 1000+ compounds

Comprehensive MS/MS MRM Database

• Factory setup and check out with pesticide mixture Pre-config. and factory

setup / tested

New

Multi-Residue and Target List Analyzer Pesticide & Environmental Pollutants 2.0 Analyzer


Benefit of Backflushing It ensures the highest data quality with GC/MS/MS!

16

Increased Productivity:

• Selectivity of MS/MS eliminates “dirty matrix” - not seen but exists

• Demanding LOD necessitates users inject larger sample volumes

• Many late eluting peaks are not “chromatographically ideal” and leave a

residue throughout the column

• Heavy matrices contaminate the source faster – performance is LOST!

Slight decrease in sensitivity as a trade-off due to the additional flow

introduced from the Aux EPC.

Agilent Solutions

1075 Pesticides & Environmental Pollutants

Average of 8 MRM transitions for each compound

-- provides alternatives to minimize matrix interferences

-- includes relative intensities for all transitions

Retention times (RT) and indexes (RI) for three GC methods

-- Fast method (20’) Full screening method (40’ CF) and Flexible method: (40’ CP)

Comprehensive and Flexible MRM Database Pesticide & Environmental Pollutants 2.0 Analyzer

Compound Classification Total Number

Pesticides (fungicides, herbicides, insecticides, rodenticides and others) 675

Breakdown Products 42

Deuterated Compounds 6

Polybrominated Diphenyl Ether (PBDE) 4

Polybrominated Biphenyl (PBB) 1

Polychlorinated Biphenyl (PCB) 209

Polycyclic Aromatic Hydrocarbon (PAH) 26

Phthalates 17

Additional Semi-volatile Pollutants 94


Multi-residual, target list analyzer example 1000+ Pesticide and Environmental Pollutants

Ordering Structure

G3445B Agilent 7890 Series:

• G3445B Option # 002 or 003 (Fast oven)

• G3445B Option # 150 or 151 (MMI)

• G3445B Option # 411 GC/MS/MS multiresidue

Analyzer w. CP method)

Agilent 7693 Series:

• G4513A 7693A Autoinjector Module

• G4514A 7693A Autosampler tray module

Agilent 7000 Series:

• G7010AA 7000B Triple Quad GC/MS/MS EI bundle

• G7010AA Option # SP1 System indicator ($0)

Note:

For GC/MS/MS P&EP analyzer option: includes 1000+

compd MRM DBL

But in GC/MSD analyzer option: it doesn’t include DRS

SW or DRS DBL, need to be ordered separately

G3440A Option # 301 (Three channels of auxiliary EPC)

G3440A Option # 305 (Factory plumbing For quick installation)

1x19091S-433UIHP-5MS Ultra Inert 30m, 0.25mm, 0.25um

1xG3186-60580 Purged Ultimate Union Assy, Inert

2xG3182-00100 CPM Union, Oven Wall Clip

4xG2855-20530 Internal nut for micro fluidic product

1xG2855-90025 CD - CFT Accessories Info & Calculators

1x0100-0680 Tee-Union, 1/16inch Brass

1xG3430-60550 Tubing Block Assembly

1x0100-1329 Ferrule, 1/16" Vespel 10/PK

1x5080-8853 Ferrule Graphite 320um 0.5mm id 10/PK

1x5188-5365 Liner O-Ring, Non-Stick 10PK

1x5183-4759 Agilent Advanced Green 11mm septa, 50/pk

1x5188-5361 Siltite Ferrules,0.25mm column,10 pack

1xG2855-20555 MSD,Ultimate Union and QuickSwap swaging nu

1xG1099-20030 Column Installation tool for 5973/5975

1xG2855-60200 Ferrule Pre-Swage Tool,Capillary Flow

1x5190-0494 Pesticide Checkout std 100ug/L 3x1ml

1xG3186-00201 Standoff Bracket for CFT Unions

1xG1580-90200 Customer data CD

2xG2855-60570 Plug for microfluidic manifold or unions

1xG2855-40001 Magnifier, 3x, 6x, paddle, plastic

1xG3430-20011 Aux Frit Case - Zero Restriction

1x160-7625-5 Silconert treated Inert Fused Silica 5m, 0.15mm

1x0100-2354 1/16"od x .010" id x 1m Tube, 316 SST

1x5190-2293 Liner,UI,splitless,sngle taper,glasswool

1xG9250-64000 Pesticide & Envirn Pollutant MRM DB

System pluming and setup according to application

Analyzer Manuel CE specialist onsite I&F

Confirm with Confidence:

analyzer option includes required

hardware, method specific

consumables and analytical

methods (3).

Plus 1000+ compound MRM DB


Benefit of having 8 transitions in the database Example: Analyze Atrazine in Different Matrices

Top 2

transitions

• Background interferences can vary significantly from matrix to matrix

• Top 2 MRM transitions (most responsive) may not be the most selective transitions

• Quantitation results can be inaccurate due to poor choice of transitions

19

Pepper Orange Pear Flour Spinach Strawberry

target analyte interference

from matrices

Agilent Solutions

No Need of extensive MRM transition development

Optimized MRM Transitions for Atrazine in DB


Evaluate Atrazine Top 4 MRMs in Different Matrices


Benefit of having 8 transitions in the database Example: Analyze Atrazine in Different Matrices

Optimized

transitions

• Alternative transitions eliminates the interference

• Minimum interference for improved analytical performance

22

Pepper Orange Pear Flour Spinach Strawberry

target analyte

Agilent Solutions

Other Multi-residue, Target List Analyzers Factory Configured and Chemically Tested

Facilitates utilization of Agilent’s state-of-art technologies

G3445B Opt. Analyzer Description

411 GC/MS/MS Pesticide Analyzer (CP-PC-BF)

412 GC/MS/MS Pesticide Analyzer (CF-MC-BF)

421 GC/MS/MS PAH Analyzer

451 GC/MSD Pesticide DRS Screening Analyzer

455 GC/MSD/FPD/ECD Pesticide Analyzer

461 GC/MSD Semi-Volatiles Analyzer

465 3 in 1 Environmental GC/MSD Analyzer

471 GC/MSD Toxicology DRS Analyzer w. NPD

475 GC/MSD(Diff) Toxicology Analyzer (CF)

481 HSS GCMSDFID Residual Solvents Analyzer

482 FAME in JetFuel Analyzer


Market Situation

Unreliable supply of helium worldwide and increasing prices have

customers seeking alternative carrier gas solutions


Agilent’s COMPREHENSIVE Solutions Carrier Gas Decision Tree

Consider migration to H2 Consider migration to N2 He Conservation

GC/MS specific H2

considerations

Are you willing to convert

to alternative gasses?

Is the Application based on

GC or GC/MS?

Does the current GC method

have more than enough resolution?

No

No

Yes

Yes

GC/MS GC


Introduce Programmable He Conservation Module Helping Your Facility Address Issues Related to Helium

Combines with 7890B Sleep-Wake feature

Automatically switches carrier gas supply to N2 during GC idle time

TWO Critical Components

• Carrier gas switching EPC module

• 7890B Sleep/Wake Feature

Plus …

• Chromatographic grade N2 gas


Carrier Gas Switch EPC Module Ensures Business Continuity

To Inlet EPCs Purge

He in

N2 in

Flow channel inside

the bridge block

Std. Aux EPC

28

• Built on 5th generation EPC

• Fully integrated and

controlled by Agilent CDS

• Purge channel prevents

cross contamination of gases

• Precise pressure control

between tank and GC

• In AWAKE mode, switches

between gases within 15-

30min for most detectors

Agilent Solutions

How it Works ? Helium Saving Mode (Nitrogen Carrier, or Sleep Mode)

29

Bridge Block

To GC Inlet

EPC

AUX EPC 1

Nitrogen

70 psig

AUX EPC 3

Purge Vent

10 psig

AUX EPC 2

Helium

0 psig

24.2 mL/min N2

(< 0.2 mL/min)

He 25.2 mL/min N2

1.0 mL/min (out)

Helium OFF, Nitrogen ON at 70 psig

Agilent Solutions

GC/FID Wake Method: 15-30 Min

GC/MS Wake Method: 15-30 Min

Some other detectors may need longer

30

How it Works ? Normal Operation Mode (Helium Carrier or Wake Mode)

To GC Inlet

EPC

AUX EPC 1

Nitrogen

0 psig

AUX EPC 3

Purge

1.0 psig

AUX EPC 2

Helium

80 psig

24.2 mL/min He

(< 0.2 mL/min) N2 25.2 mL/min He

1 mL/min (out)

Bridge Block

Helium ON at 80 psig, Nitrogen OFF

Agilent Solutions

How It Works: Configuring Sleep/Wake Operation Simple, Straight Forward Setup


Performance: No Change in Chromatography After

N2 Carrier Sleep Method. GC/FID Analysis

32

Day 1 - Original He carrier gas run

Day 2 – First He carrier gas run after overnight N2 Sleep.M method

Day 3 – First He carrier gas run after overnight N2 Sleep.M method

14 16 18 20 22 24 Min.

Agilent Solutions

Performance: Pass MS Tune Within 15min After

Switching From N2 To He As Carrier. GC/MSD

5000

50000

500000

5000000

0 5 10 15 20

Co

un

ts

Time (min)

Nitrogen Background

5 mL/min He

2 mL/min He

Counts of Nitrogen Ion

Time (min) 5 mL/min He

Relative to

Saturation 2 mL/min He

Relative to

Saturation

3 1735168 20.69% 8388096 100.00%

4 1033280 12.32% 4959232 59.12%

5 590080 7.03% 1618944 19.30%

6 354112 4.22% 722944 8.62%

7 228480 2.72% 333696 3.98%

10 56984 0.68% 102576 1.22%

15 9052 0.11% 17080 0.20%


Carrier Gas Savings Calculator Save Helium by > 90% compared to a typical use

Helium supplies last MUCH longer

Gas saver mode: 9 times longer

N2 standby: another 3.3 times more

Total save: last 30 times longer

For instruments routinely in stand-

by, savings are even greater

“Sleep more, Save more”

Carrier Gas Savings Calculator

Change values in gray boxes to calculate savings for your operating parameters

Method:

Column:

Gas Flow Conditions

He Carrier Flow (mL/min): 1.2

He Split flow (mL/min): 190

Gas Saver Flow (mL/min): 20

Gas Saver On (min): 3

Run Time(min.): 30

Gas Volume in Cylinder (L): 8000

Runs per Day: 15

He Cylinder Cost ($): 300

N2 Cylinder Cost ($): 60

Parameter No Gas Saver Gas Saver OnGas Saver +

N2 Standby

Daily He Usage (L) 275 30 9

He Cylinder Life (days) 29 265 873

Daily N2 Usage (L) 0 0 21

N2 Cylinder Life (days) 0 0 381

Yearly He Cost ($) 3,769 413 125

Yearly N2 Cost ($) 0 0 57

Yearly Total Gas Cost ($) 3,769 413 183

ASTM D7504 - Analysis of Trace Impurities in Monocyclic

HP-INNOWax, 30 m x 0.32mm ID x 0.25 um


Customer Benefits Ensuring Business Continuity

• Seamless integration

Fully integrated with 7890B and CDS (OpenLAB, Mustang, Mass Hunter)

Method includes carrier gas ID and set points for compliance and transfer

Easily implemented using new OpenLAB Sleep/Awake functions

• Greater reliability

Based on proven 5th generation AUX EPC

Warning from 7890 if set points are not reached

For hydrogen users, nitrogen substitution when GC idle

• Greater performance

Purge channel prevents cross contamination of gases

Acts as an intermediate pressure regulator from the tank to inlet EPC to ensure greater analytical precision

35

Thermo

PE

No more worries about He shortage,

No change to method, eliminates revalidation of analytical method !

Agilent Solutions


Juan Aybar

GC & GCMS Marketing Program Manager

Agilent Technologies

[email protected]

+34 626622673

Gracias por su

Atencion

?

mailto:[email protected]



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