Innova Biosciences Corporate Presentation_v20_inc Pi

© Innova Biosciences ltd. 2015. All rights reserved

Quality – Consistency – Expertise


What is Lightning-Link® technology?

The worlds fastest, easiest to use and most efficient conjugation technology!

• Only 30 seconds hands-on time! • Over 50 labels available including:

Enzymes, fluorescent proteins / dyes, tandems, biotin & streptavidin

Lightning-Link®

Antibodies – Proteins – Peptides

Fast – Easy-to-use – Reliable


What is Lightning-Link® technology?

• 100% antibody recovery

• Fully scalable from R&D (10µg<) to Production / Manufacture (>1g)

• Virtually eliminates batch to batch variability

• Coupling of the label to antibodies, proteins or other biomolecules

• Covalent conjugation ensures long term stability

• Available as traditional Lightning-Link® (3 hour incubation) or new Lightning-Link® RAPID (15 minute incubation)

Lightning-Link®


Lightning-Link®

Lightning-Link® Alkaline Phosphatase (AP)

Lightning-Link® Glucose Oxidase

Lightning-Link® Horseradish Peroxidase (HRP)

Enzyme labeling kits

Lightning-Link® Avidin

Lightning-Link® Biotin

Lightning-Link® Streptavidin

Biotin/Streptavidin labeling kits


RPE – blue = Ab supplier red = LL

Anti-human CD54 (HCD54) was purchased in pre-conjugated form and in unconjugated form for conjugation using Lightning-Link® kits

Leading Ab supplier Lightning-Link® Overlay

FITC – blue = Ab supplier red = LL

A mouse monoclonal antibody, clone HCD54, specific for human CD54 was purchased from a commercial source in both unconjugated and RPE or FITC conjugated formats. The unconjugated antibody was linked to RPE or FITC using a Lightning-Link® kit, and the two conjugates were compared in flow cytometry staining human peripheral blood lymphocytes, where the blue curve shows unstained cells. Lightning-Link® conjugated antibody is shown in the middle, and commercially conjugated antibody on the left hand side. The right hand histograms show an overlay of the positively stained cells.

Lightning-Link® Comparative Data


Anti-human CD20 (clone L26) was conjugated to HRP using Lightning-Link® kits, and used in IHC in comparison to a traditional indirect technique


Unconjugated L26, visualised with Dako EnVision HRP conjugated L26, 1/100

Samples shown are formalin-fixed, paraffin-embedded human bone marrow. CD20 is a B cell specific marker


Anti-human IgG monoclonal antibody was purchased pre-conjugated to alkaline phosphatase from a commercial supplier. The same antibody was purchased in un-conjugated form and then conjugated to alkaline phosphatase using a Lightning-Link® kit.

The Lightning-Link® conjugate demonstrates enhanced titre and sensitivity.


Alkaline Phosphatase anti-Human IgG(Fc) Conjugates Analysed By ELISA

100 1000 10000 100000 10000000.0

0.5

1.0

1.5

2.0

2.5

3.0 LL Kit generatedsecondary antibody

LL Kit generatedantibody with no antigen

Commercially availablesecondary antibody

Commercially availablesecondary antibodywith no antigen

Conjugate dilution

Abs

orba

nce

at 4

05nm

Alkaline Phosphatase anti-Human IgG(Fc) Conjugates Analysed By ELISA

(conjugate at 1:10000 dilution)

10 100 10000.0

0.5

1.0

1.5

2.0LL Kit generatedsecondary antibody

Commercially availablesecondary antibody

Antigen / well (ng)

Abs

orba

nce

at 4

05nm


Lightning-Link® Rapid Kits

Same Lightning-Link style conjugation kits, but incubation is reduced to just 15 minutes

Conjugates are equal or higher quality than the traditional Lightning-Link conjugates

Antibody or protein conjugates can be generated in just 20 minutes!

Lightning-Link® Rapid Biotin is also available


Reliable, high quality conjugations at optimal scale for manufacturers to enable you to focus on assay development, rather than conjugation chemistry.

Bespoke solutions and optimisation are available to maximise your benefits from our technology.

Immunoreagents partnershipBelgian Volition partnership

Conjugate preparation for manufacturers

http://www.innovabiosciences.com/latest-news/lightning-link-in-licensing-deal-enhances-immunoreagents-product-offering-to-the-diagnostic-industry.html

http://www.innovabiosciences.com/latest-news/volitionrx-to-use-innova-biosciences-biotin-technology-in-its-epigenetic-research-kits.html


Conjugated Antibody Library

Launched with an initial range of cardiac specific biomarkers, one of the fastest growing areas of IVD development.

• 28 specificities

• Comprising 209 different antibodies

• 24 different conjugated versions of each

• 5016 new products!


InnovaCoat® GOLD

• Proprietary surface coat

• Ultra-Stable

• Enhanced assay sensitivity

• Covalently link antibodies and analytes

• Simple conjugation processes

• Different surface chemistries

• Custom labelling service available


A comparison of antibody conjugation using InnovaCoat® GOLD against that using traditional passive conjugation techniques, with naked gold colloid, showing enhanced signal intensity with InnovaCoat® GOLD. Anti-IgA antibodies were labelled with 40nm gold particles, and used to measure IgA concentration in a competitive lateral flow inhibition assay: IgA in the sample competes with that bound to the nitrocellulose on the lateral flow strip and therefore as the concentration in the sample increases the signal intensity decreases.

The data in the graphs above demonstrates the enhanced signal intensity observed when using InnovaCoat® GOLD in comparison to passively conjugated naked gold, under identical conditions. The curve resulting from the use of InnovaCoat® GOLD provides significantly increased sensitivity for measuring the IgA concentration. Furthermore, the covalent attachment of the antibody to the InnovaCoat® GOLD provides enhanced stability and prevents aggregation of the conjugate.

InnovaCoat® GOLD


Surface chemistries for orientated labelling!

• InnovaCoat® GOLD-Hydrazide 20 OD 40nmFc directed labelling of IgG antibodies

• InnovaCoat® GOLD-Maleimide 20 OD 40nmOrientated labelling of Fab’ fragments

InnovaCoat® GOLD


InnovaCoat GOLD product range and applications

InnovaCoat® GOLD


InnovaGOLD®


InnovaGOLD®

Electron microscopy comparisons of InnovaGOLD® with two leading competitors, demonstrating improved consistency of both size and shape.


Thunder-Link®

New immunoassay techniques utilize oligonucleotide – antibody conjugates to combine the amplification power of PCR with the specificity of antibodies, resulting in increased assay sensitivity.

BUT traditional techniques for conjugating antibodies to oligonucleotides are costly, time consuming, require expert knowledge and often contain unbound material!

The solution:


• Unbound oligo removal step included• Unidirectional technology• Covalent bond• End user chooses oligo sequence and

antibody.• Oligo sizes (HPLC purified) between

10-120 bases.• Amine group positioned at the 5’ or 3’.• Positive control included.• All necessary components for a

successful conjugation supplied.

What is the Thunder-Link® technology?

It is the most cost effective and easiest to use Oligonucleotide-Antibody conjugation kit that exists!

Thunder-Link®


ELISA Oligo-linked immunosorbentassay

Detection Limits µg to pg pg to fg

Amount of starting material required

µl pl

Combines the specificity of antibodies with the amplification power of PCR

Thunder-Link®


Antibody, protein or peptide buffer considerations…

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, and azide) that interfere in labelling reactions.

We have developed a range of purification kits which allow for easy and rapid purification of antibodies from any buffer formulation, including:

• Tissue culture supernatant (TCS) • Serum and ascites fluid• Concentration of antibodies and proteins • Buffer exchanges

The AbSelect and AbPure ranges ensure that your antibody is 100% compatible with our conjugation technologies.

Purification and Clean Up Kits


AbSelect Antibody Purification Kits


AbPure Antibody Purification Kits


PiColorLock™ phosphate detection reagent changes colour in the presence of inorganic phosphate (Pi).

This can be exploited to measure any enzyme that generates Pi including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins.

What is the PiColorLock® detection reagent?

Phosphate Detection Reagent


Pi contamination

Non-enzymatic decay

Precipitation

Issues with traditional colorimetric assay dyes

PiColorLock®


Non enzymatic decay – (high background)Affect of incubating ATP (without any added enzyme) with three commercial dye based reagents.

PiColorLock ™ completely suppresses non-enzymatic background signal

PiColorLock ® overcomes:

Pi contamination of media – (high background) Removal of 1 mM P i from buffers

of varying pH with P iBindTM resin

Glycine

pH 2

.3

Aceta

te p

H 5

MES p

H 6

MOPS p

H 7

Hepes

pH 7

.5

Tris

pH 8

No re

sin

Blank

(wat

er)

0

1

2

3

A650

Dialysis of samples – fairly simple, however slow process

Desalting – can be difficult and limited to small volumes, but can be fairly quick

PiBind ™ resin – very simple process, and very fast

PiColorLock®


Precipitation

PiColorLock ™ has special additives that enhance the stability of dye complexes

PiColorLockALS

Competitor B

Competitor A

PiColorLock ™ offers much higher sensitivity

PiColorLock ® overcomes:

PiColorLock®


PiColorLock® product range:

High Throughput Colorimetric ATPase Assays (inc. PiColorLock)• This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included

in the PiColorlock kit and more) and is ideal for high throughput drug screening.

High Throughput Colorimetric GTPase Assays (inc. PiColorLock)• As above, this non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything

included in the PiColorlock kit and more) and is ideal for high throughput drug screening.

PiColorLock Phosphate Detection System• PiColorLock is a phosphate detection reagent for measuring phosphatases, ATPases, GTPases and other enzymes that release

inorganic phosphate (Pi). The PiColorLock Phosphate Detection System includes PiColorLock reagent (high sensitivity) as well as Accelerator, Stabiliser and Pi Standard.

10mM Lyophilized ATP• These lyophilized 10mM ATP vials contain ultra high quality ATP to ensure the lowest possible assay background. (Also supplied as

part of the ATPase assay kit).

PiColorLock®


• In-house conjugation• Conjugation optimisation• Bespoke formulations• Bespoke kit size• Batch validation• Batch reservation• Antibody fragmentation

Custom Products & Services


Innova Biosciences is committed to working with you to develop a long term mutually beneficial & commercially successful relationship.

We will identify your specific requirements, in all aspects of bioconjugation, and provide optimal technical and commercial solutions.

Partnering with Innova

Innova Biosciences Corporate Presentation_v20_inc Pi

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