Effect of climate on plant growth and level of adaptogenic compounds in Rhodiola rosea, Leuzea chartamoides and Serratula coronata Inger Martinussen, Vladimir Volodin, Gunnlaug Røthe, Kirsten Jakobsen and Hermod Nilsen

BARENTSHERBS

Controlled experiment in a phytotrone

24 h 12 h

9°C

15°C

21°C 21°C

21°C day9°C

night

Temperature effects on growth of Rhodiola

TEMP CSHOOTS

P<0.0001D.W. (g) ROOT

P<0.007**

9 28.3 B 17.6 BA

15 30.3 B 21.9 BA

21/9 40.3 A 24.1 A

21 12 h 12.3 C 13.2 B

21 24 h 22.5 B 15.4 BA

N = 30 pr treatment1 clone Rovaniemi1 clone Akershus1 clone Tromsø

Temperature effects on adaptogens in Rhodiola

TEMP C

Cinnamic alcohol

P<0.796

RosavinP<0.873

TyrosolP<0.001**

Salidroside

P<0.830

Total rosavinesP<0.833

9 0.75

A 3.02 A 0.15 A 2.42 A 5.59

A

15 0.67

A 2.98 A 0.11 AB 3.34 A 4.96

A

21/9 0.72

A 2.66 A 0.09 B 2.85 A 4.66

A

21 12 h

0.61

A 3.29 A 0.06 B 3.27 A 4.97

A

21 24 h

0.66

A 2.94 A 0.06 B 2.98 A 4.75

A

Clonal differences!

•The northernmost clone best•The trends are the same – the levels different

Temperature effects on Leuzea

chartamoides

TEMP C HIGHT (cm) P<0.0001***

FLOWERING (NUMBER)

P<0.0001***

D.W. (g) ROOT P<0.0001***

9 53.0 C 0 C 1.7 C

15 68.5 A 0.4 B 5.5 A

21/9 69.8 A 0.5 B 4.2 B

21 12 h 30.2 D 0 C 1.4 C

21 24 h 59.7 B 1.3 A 2.4 C

N = ca. 40 pr treatment1 clone from Mikkili1 seed population from Mikkili

Ecdysteroids in Leuzea chartamoides

•N = 8 pr treatment – combined from 5 samples

•Low at 9 ◦C•Highest at 15 ◦C and decreases at 21 ◦C

•Negative effect of long days at higher temperatures?

Temperature effects on growth of Serratula coronata

TEMP C HIGHT (cm) P<0.0001***

FLOWERING P<0.0001***

D.W. (g) LEAF P<0.0001***

9 20.3 C 0 C 0.8 D

15 93.1 A 1.4 BC 11.4 B

21/9 80.0 B 2.9 A 8.2 C

21 12 h 17.2 C 0 C 0.9 D

21 24 h 81.8 B 2.0 BA 16.0 A

N = 40 pr. treatment2 seed populations

Ecdysteroids in Serratula coronata

•N = 8 pr treatment – combined from 5 samples

• Short days as negative

•Increase in concentration from 9◦C to 15 ◦C

Summary & conclusions

• Long days necessary for growth and flowering• Short days reduced the concentration of

ecdysteroids in Serratula (NB – tested only at high temperature)

• Long days reduced the concentration of ecdysteroids in Leuzea (NB – tested only at high temperature)

• Low temperature significantly increased the amount of tyrosol in Rhodiola and results shows a higher concentration of the other adaptogens also at low temperature (9◦C & 15◦C), however, not significant

• The northernmost clone had the highest content of adaptogens at all treatments

• The intermediate temperature of 15◦C gave the highest amount of ecdysteroids in both Leuzea and Serratula

THANKS!• Institute of Biology, Komi Science Centre, Russia• MTT Rovaniemi, Finland• MTT Mikkili, Finland• Bioforsk – Svanhovd, Norway• Bioforsk – Kise• Bioforsk – Holt• UiTø

The Norwegian Barents SecretaryInterreg IIIAAgronomy County offices in Troms and Finnmark