Information Management

The old fashioned way!

Gary R. Skuse, Ph.D.Director of Bioinformatics

grssbi@rit.edu

Essential Cell Biology : An Introduction to the

Molecular Biology of the Cell

by Bruce Alberts, Dennis Bray, Alexander Johnson, Julian Lewis, Peter Walter, Keith Roberts, Martin Raff

Garland Pub; ISBN: 0815320450

All Illustrations taken from:

Molecular Biology is about Information

Alice

Information Flow within Cells

Spatial Flow of Information

DNA is Organized into Chromosomes

Painted Chromosomes

Electron Micrographs of Chromosomes

Chromosomes are Comprised of DNA

Packaged with Proteins

Chromosomes Contain Large Quantities of DNA

DNA can Replicate Itself

DNA Replication is Uncommonly Accurate

Information in DNA is Transcribed into RNA

There are Different Types of RNA

Gene Organization in Prokaryotes

Translation of Prokaryotic and Eukaryotic RNA

DNA Serves as a Template for Transcription

Eukaryotic Genes are Not Contiguous

RNA Splicing is Sequence Directed

Alternative Splicing Leads to Multiple Proteins from One Gene

RNA Processing, Like All Things Biological, is

Complex

Gene Expression is Regulated Differently for Different Genes

RNA is Translated into Protein

The Code Must be Parsed Accurately

Linear Strings of Amino Acids Interact

Proteins Fold to Form 3-D Structures

We can Depict Protein Structures in

Many Ways

We can Depict Protein Structures in

Many Ways

We can Depict Protein Structures in

Many Ways

We can Depict Protein Structures in

Many Ways

Protein Shape Leads to Function

Protein Shape Leads to Function

Proteins may Change Shape as they Function

Genes are Somewhat Modular

Protein Structure Reflects that Modularity

Preparation of DNA for Cloning

Cloning of DNA

The PCR