Influence of hyperactivity treatment at the collagenous tisue
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Transcript of Influence of hyperactivity treatment at the collagenous tisue
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INFLUENCE OF HYPERACTIVITY
TREATMENT AT THE COLLAGENOUS
TISSUE DENSITY OF THE MUSCLEATTACHMENT OF LONG BONES
DURING GROWTH
NAME : ARIE PRADIPTA
NIM : 030.07.031
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TOPIC
Collagen is the major structural protein of the body
Approximately 33 percent of protein in the body is collagen
In fact, bone is also composed of collagen combined with certain minerals such as
calcium and phosphorus
Bone is a tissue composed of cells and is dominated by collagen extracellular
matrix (type I collagen) are known as osteoid.
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METHODS
A method for this study building on experimental animal with cross-sectional
approach
During the migration process, there are changes in the density collagen
tissue
As experimentals animal used 120 male rats
- Age 6 weeks- Weighing 70 grams
Doing the experiment in hyperactivity treatment during 2 until 6 months, may
be used to explain the results of primary research
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METHODS
Preparations for this experiment taken from musculus pectineus and
musculus rectus femoris from experimental animal, that is the muscle
attachment of the long bone during growth being checked under microscope
Preparations workable by the method of paraffin and made cuts nearly
parallel to the axis bone length and preparations 6 microns thick slices and
polished with Azan Mallory staining
On microscopic preparations of m. pectineus and m. rectus femoris
attachment assessed the level density of collagen tissue is qualitatively by
comparing the color of the collagen tissue with the guidelines previously
determined color density values with degree of color intensity values, ie
less dense (+) with a score of 1quite dense (++) with a score of 2
the densely (+++) with a score of 3, drawn from the entire preparation.
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METHODS
Three example of the level density can be seen in the picture below:
Figure 1: Microscopic images of longitudinal cross section of the
muscle tendon attachment on the bone density that show fibraecollagenous less dense. Mallory azan staining. Information: 1.Fibraecollagenouse 2. Cartilage cells
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METHODS
Three example of the level density can be seen in the picture below:
Figure 2: Microscopic images of longitudinal cross section of the
muscle tendon attachment on the bone density that show fibraecollagenous pretty solid. Mallory azan staining. Information:1.Fibrae collagenouse 2. Cartilage cells
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METHODS
Three example of the level density can be seen in the picture below:
Figure 3: Microscopic images of longitudinal cross section of the
muscle tendon attachment on the bone density showed a hiperdensity fibrae collagenous. Mallory azan staining. Information:1.Fibrae collagenouse 2. Cartilage cells
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METHODS
The Implementation Of The Research
All of the experimental animals are placed to live inside the cage with 90cm
long and 30cm height of size, for 10 rats in about 2 until 6 months after
treatment
Rats are fed with food 521 and added with 100gr mashed corns each day for
every 10 rats and also drink ad libitum
Experimental animals are divided in to two groups: 1. Controls (60 rats) 2.
Hiperactivity (60 rats)
The value of the collagen tissue density is taken at the M.pectineusattachment on femur proximal part and M.rectus femoris on tibia proximal
part, on the control group and hyperactivity group, each after 2 months and
6 months of treatment on the both group mentioned before
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METHODS
Measurement Result Of The Collagen Tissue Density On The Muscle
Attachment To The Bone
Table XIIa: The average of the collagen tissue density on control group 2months and 6 months
Time of
Treatment
The Average of Density Collagen Tissue
2 months 6 months
Muscles
Attachment
X S.B X S.B
M.pectineus 1,567 0,496 1,9330,249
M.rectus
femoris
2,600 0,554 2,8330,373
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METHODS
Table XIIb: The average of the collagen tissue density on hyperactivitygroup 2 months and 6 months
Time of
Treatment
The Average of Density Collagen Tissue
2 months 6 months
Muscles
Attachment
X S.B X S.B
M.pectineus 1,967 0,180 2,000 0,000
M.rectus
femoris
2,700 0,458 2,767 0,423
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METHODS
Table XIIc: Result of Fisher Exact test from the differences of the collagentissue density between 2 months and 6 months
Group Control Hyperactivity
Muscles Attachment
M.pectineus p>0,05*
*the differences not to shown
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METHODS
Table XIIc: Result of Chi-kuadrat test from the differences of the collagentissue density between 2 months and 6 months
Muscles
Attachment
Group
Control Hyperactivity
M.pectineus X2=10,755
p0,05*** p>0,05***
*the differences shown
**the differences very shown
***the differences not to shown
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RESULT
Hyperactivity treatment causes collagenous tissue density show fibrae
collagenous less dense, pretty solid, and hiper density
The addition of muscle activity during the development will increase the
growth of tendon.
In hyperactivity treatment (physical exercise) there is a correlation between
collagenous tissue density and the muscle attachment of long bones wassignificantly with the degree of migration of muscle attachment on the bone
during growth.
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RESULT
The treatment makes collagenous tissue density show fibrae collagenous:
Less dense
Pretty solid
Hiper density
Assessed the level density of collagen tissue is qualitatively by comparing
the color of the collagen tissue with the guidelines previously determined
color density values with degree of color intensity values, ie1. less dense (+) with a score of 1
2. quite dense (++) with a score of 2
3. the densely (+++) with a score of 3, drawn from the entire preparation.
In the hyperactivity treatment was found that the absolute color densityvalues with degree of color intensity values of the collagen tissue density at
the muscle attachment
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CONCLUSION
The influence of hyperactivity treatment for the experimental animal to get
the changes of collagenous tissue density of the muscle attachment of long
bones during growth.
It was found that in the hyperactivity rats, a change of collagenous tissue
density was detected, whereas none in the control group.
The change of the collagenous tissue density show fibrae collagenous lessdense, pretty solid, and hiper density.
In conclusion, there was a convincing the influence of hyperactivity
treatment at the collagenous tissue density of the muscle attachment of long
bones during growth. That influence to the muscules gain mass and will addthe sacroplasma. Hyperactivety treatment causes muscle hyperplasia or
hypertrophy that increases muscle volume and bone length change.
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REFERENCES
.
1. Boer A 1980 a. Pertumbuhan Femur dan Tibia Mencit akibat Perlakuan
Bipedal. Pertemuan Nasional Anatomi V Semarang. Boer A 1980 b.
Pertumbuhan dan Perkembangan Beberapa Otot Tungkai Belakang
Mencit akibat Perlakuan Bipedal. Pertemuan Nasional Anatomi V
Semarang.
2. Chaui R et al. 2004. A Little Exercise Goes A long Way. Child Health
Alert. Vol: 22, p: 2-3. National Library ofMedicines Midline.
3. Cheema TA et al. 2006. Measurement of Rotation of the FirstMetacarpal during Opposition using Comperted Tomigraph. Journal of
Hand Surgery. Vol: 31, Iss: 1, p: 76-9.
4. Cooper RR and Misol S 1970. Tendon and Ligament Insertion. A Light
and Electron Microscopic Study. J. Bone Joint Surgery 52A (1) : 1-20.
5. Dill DB et al. 1964. Handbook of Physiology, Adaptation to theEnvirontment. Section 4, pp.75. American Physiological Society.
Washington, D.C.