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Clean Water Healthy WorldBill Eggers, CEP, CWB, PWSVP Science & TechnologyMarch 29, 2016bill.eggers@aquafiber.com

Vision: Clean Water Healthy WorldMission:AquaFiber fulfills a promise to restore surface waters impaired by nutrients and other harmful pollutants. Photo: AquaFiber - Lake Apopka, 2009

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AQUAFIBERA Technology-based, clean-tech companyfocused on surface water remediationHarvesting NATIVE ALGAE AND SUSPENDED SOLIDSUsing aqualutions Dual-nutrient (TP/TN) technologyModifIED Dissolved Air Flotation (DAF)Coupled to a traditional and trade-secret treatment trainCapitalized Privately

Lake Apopka Effluent

A Brief History2000 incorporated to grow periphyton for alternative packaging material2002 awarded sfwmd contract to study nutrient removal2003 constructed pilot facility on lake Apopka (roper site)2004 demonstrated ability to remediate lake Apopka water to NELAC drinking water standards2004 Research begins on aqualutions2007 aquafiber rolls out aqualutions2007 awarded sjrwmd lake Jesup contract2008 awarded darpa contract with saicDeveloped mobile technology (AquaKnight )2009 operations begin at lake jesup2011 fdep acknowledges aqualutionsas effective and safe2013 Developed passive technology to also remove unconsolidated muck2014 first phase of Contract with SJRWMD completed successfully

AquaLutionsCustomized FOR RESTORATION TARGETSOptimized for large, hypereutrophic surface waters with heavy legacy loadsProven at Commercial ScaleEffective and efficientSmall Land FootprintReliableScalable SustainableEnvironmentally Safeleast expensive Life-cycle cost on market

Lake Jesup Biomass

Phosphorus removal and more

Aqualutions can:Reduce Total Phosphorus (TP) loadings by more than 75% on averageReduce Total Nitrogen (TN) loadings by more than 45% on averageReduce Total Suspended Solids (TSS) by more than 70% on averageRestore water clarityRemove harmful, toxin-producing cyanobacteriaReduce Chlorophyll aimprove Dissolved Oxygen concentrationRemove unconsolidated muck and legacy loadsProduce a beneficial biomass

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24 x 7, 350-day operation

Influent (raw) and effluent (treated) water sampled and analyzed flow rate and TSS recorded 600 times per hour

Autosamplers collected raw and treated water samples once per hour for nutrient testing

SJRWMD maintained its own set of samplers and data

Lake Jesup Results - Total operational PeriodApril 2009 December 2013WATER FLOWTotal Water Treated5.7 billion gallons

Mean Daily Water Flow3.7 million gallonsTOTAL PHOSPHORUSFDEP NNC for colored lakes0.050 mg/lNNC for lake Jesup bmap0.096mg/lMean INFLUENT CONCENTRATION0.163 mg/LMean effluent concentration0.033 mg/lMean removal efficiency75.1%Pounds TP removed6,449 lbsTOTAL NITROGENFDEP NNC for colored lakes1.27 2.23 mg/lNNC for lake Jesup bmap1.27 mg/lMean INFLUENT CONCENTRATION3.480 mg/LMean effluent concentration1.64 mg/lMean removal efficiency46.1%Pounds TP removed90,749 lbs

Lake Jesup Optimal RESULTS - Year 2DUAL NUTRIENT EFFICIENCY4/18/10 4/18/11Total Water Treated (350 days)1.7 billion gallonsMean Daily Flow4.9 million gallonsTotal TP Removed2,474.30 lbs(1.12 MT)

TP Removal Efficiency82.49% 15.88 SD

Total TN Removed33,659.91 lbs (15.27 MT)

TN Removal Efficiency59.08% 13.18 SD

FDEP verificationAquaFiber received a letter dated February 7, 2014 from FDEP stating:

The Department has reviewed the available information on the pilot AquaLutions Project in Lake Jesup. we believe that the aqualutions process is demonstrating success in removing phosphorus and nitrogen from the lake. Additionally, toxicity tests of the treated and untreated lake water have demonstrated that the treatment is not harmful to the lake.as a result, the department is allowing bmap credit for the phosphorus and nitrogen reduction resulting from the project on a 1:1 basis.

Lake Jesup ResultsTotal Contract PeriodApril 2009 December 2013Total Suspended Solids (TSS)Mean Influent CONCENTRATION46.1 mg/LMean Daily Influent Range8.9 mg/L min193.2 mg/L maxMean Effluent CONCENTRATION9.3 mg/LMean Removal Efficiency72.5%Total dewatered Biomass Removed13,402,584 pounds (6,079 MT)

INDIAN RIVER COUNTY mobile testing RESULTSSeptember 2014Total phosphorus0.574 mg/l to 0.002 mg/l (undetectable)removal efficiency 99%Total nitrogen3.26 mg/L to 1.22 mg/lremoval efficiency 63%

Potential Biomass partnershipsSoil AmendmentVitagOld CastleEvans AgricultureAlternative fuelsJP-8 Jet Fuel (and precursors)Hydrogen GasLiquid Fuel AlternativeAlternative energy Feed StockAnaerobic digestiongasificationGreen Building materialsPlastics alternativesNeutra/PharmaceuticalsCO2 Sequestration

Biomass-filled Geotubes

Indian river lagoonTARGET HEAVY LOADS SMARTLY aqualutions TO REMEDIATE SOURCE CANALSREMOVE MUCK FROM BACKWATERS

Comprehensive everglades restoration plan

Add aqualutions as polishing for stasExtend the lifetime and efficiencyallow stas to function as designed

In addition to the St johns river and lake jesup, this technology can be used for other major regional restoration projects

AquaLutions is the perfect solution for many of Floridas other large, impaired water bodies.

The image on the left is from ORCA and shows where nutrients are building up in the IRL; in those small canals and backwaters that feed the IRL where decades of septic tank and direct stormwater runoff have caused large accumulations of nutrients and muck. AquaFiber has tested its process successfully on the waters of the Indian River Lagoon where AquaLutions could be deployed to treat the hotspots.

The picture on the right depicts a major component of the states Everglades restoration plan. Several STAs will be built to remove nutrients. Those STAs will be fed with water from the FEBs. The water from these FEBs will be dirty and should be cleaned as much as possible so the STAs can function as they are designed, a final polishing before the water enters the Everglades. Without pre-treating the water, the STAs will reach their carrying capacity and require excavation more often than desired. AquaLutions has proven it could reduce the load and do much of the heavy lifting, so the STAs wont become overloaded.15

Photo: Sherry Brandt-WilliamsQuestions