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Journal/book title Volume Issue Page

Scientia Horticulturae

Volume 88, Iss ue 4, 31 May 2001, Pages 277-287

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doi:10.1016/S0304-4238(00)00212-0

Copyright 2001 Els evier Science B.V. All rights res erved.

In vitro induction of tetraploid ginger

(Zingiber officinale Roscoe) and its pollen

fertility and germinability

Shinichi Adaniya , and Daisuke Shirai

College of Agriculture, University of the Ryukyus, Nishihara, Okinawa

903-0213, Japan

Accepted 10 August 2000 Available online 5 April 2001.

Abstract

In vitro induction of tetraploid

ginger (Zingiber officinale Roscoe)

and its pollen fertility and

germinabil ity were investigated.

The growth of shoot tip cultures on

agar MS medium containing 2.0 mg l1 BA was greater than that of

sim ilar cultures in liquid MS medium with the same BA concentration.

In liquid medium, the combinations of 0.5, 1.0, or 2.0 mg l1

BA with

0.05 mg l1 NAA tended to enhance the growth of the cultures . The

efficiency of tetraploid induction was assess ed by treating shoot tip

explants on agar or in liquid MS medium containing 2.0 mg l1 BA,

0.05 mg l1 NAA, and 0.2% (w/v) colchicine for 4, 8, 12, and 14 days.

The total number of tetraploids induced on solid m edium was 18, but

only five in liquid m edium. On both media, the colchicine treatment for 8

days gave the maximum level of tetraploid induction. Therefore, it was

found that the treatment of shoot tip explants on agar medium

containing 2.0 mg l1 BA, 0.05 mg l1 NAA, and 0.2% (w/v) colchicine for

8 days was the mos t efficient way of inducing tetraploid ginger. Induced

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9/20/13 ScienceDirect - Scientia Horticulturae : In vitro induction of tetraploid ginger (Zingiber officinale Roscoe) and its pollen fertili ty and germinability

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tetraploid s trains, 4 Kintoki, 4 Sanshu, and 4 Philippine cebu 1 ,

had higher pollen fertility and germinability than the diploid

counterparts, i.e., in the diploid strains, pollen fertility ranged from 0.3 to

6.2% and the germination rate from 0.0 to 0.1%, while in the tetraploid

strains, pollen fertility ranged from 27.4 to 74.2% and the germination

rate from 4.8 to 12.9%.

Author Keywords: Zingiber officinale; Tetraploids; In vitro culture;

Pollen fertility; Pollen germination

Article Outline

1. Introduction

2. Materials and methods

2.1. Shoot tip culture

2.2. In vitro induction of tetraploid ginger

2.3. Pollen fertility and germinability of tetraploid ginger

3. Results and discussion

3.1. In vitro growth of shoot tip cultures

3.2. In vitro induction of tetraploid ginger

3.3. Pollen fertility and germinability of tetraploid ginger

4. Conclusion

Acknowledgements

References

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Scientia Horticulturae

Volume 88, Iss ue 4, 31 May 2001, Pages 277-287

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